Population genetics of Chrysoperla externa (Neuroptera: Chrysopidae) and implications for biological control / Genética de populações de Chrysoperla externa (Neuroptera: Chrysopidae) e implicações para o controle biológico

Green lacewings are insects with great potential to be use in the biological control of agricultural pests, but relatively few studies have attempted to understand the genetic structure of these agents, especially those of predatory insects. The purpose of this study was to characterize genetically populations of C. externa using sequences of subunit I of the cytochrome oxidase, a mitochondrial gene, and examine the population structure of this species in sampled areas in São Paulo state. The results indicate high genetic diversity but no genetic structure, detected by AMOVA analysis, and high levels of haplotype sharing in the network. These genetic patterns could be a consequence of environmental homogeneity provided by agroecosystem (citrus orchard), allowing gene flow among populations. Probably there is a unique population in the area sampled that could be used as a population (genetic) source for mass-reared and posterior release in these farms.(AU)

Crisopídeos são insetos com grande potencial para uso em controle biológico de pragas agrícolas, mas relativamente poucos estudos têm tentado compreender a estrutura genética destes agentes, especialmente no caso de insetos predadores. O objetivo deste trabalho foi caracterizar geneticamente populações de C. externa utilizando sequências da subunidade I do gene mitocondrial citocromo oxidase e avaliar a estruturação populacional desta espécie em áreas amostras no estado de São Paulo. Os resultados indicaram elevada diversidade genética e nenhuma estruturação genética, detectada pela AMOVA, além de elevado compartilhamento na rede haplotípica. Este padrão genético poderia ser uma consequência da homogeneidade ambiental favorecida pelos agroecossistemas (citricultura), permitindo fluxo gênico entre as populações. Provavelmente há uma única população, do ponto de vista genético, na área amostrada que poderia ser utilizada em criações massais e em liberações nas fazendas desta região.(AU)

Primary intraocular chondrosarcoma in a dog / Condrossarcoma intraocular primário em cão

A five-year-old male Cocker Spaniel was presented for evaluation of the right eye due to discomfort, abundant purulent discharge and progressive enlargement of the eyeball. The owner revealed that the right eye has appeared to be inflamed and smaller then the left eye for years. Ophthalmic examination revealed corneal perforation, buphthalmia and conjuctival hyperemia. Enucleating was performed due to signs of endophthalmitis and ocular discomfort. Histopathology revealed a multilobulated proliferation of chondrocytes producing hyaline cartilage with occasional pleomorphism and binucleate cells. A diagnosis of primary intraocular chondrosarcoma was done.(AU)

Foi atendido um cão, da raça Cocker Spaniel, de cinco anos de idade, com desconforto ocular, secreção purulenta abundante e aumento progressivo do bulbo ocular. Ao exame oftálmico, evidenciaram-se perfuração corneana, buftalmia e hiperemia conjuntival. Foi realizada enucleação em decorrência do desconforto ocular intenso e dos sinais de endoftalmite. Exame histopatológico revelou proliferação multilobulada de condrócitos produzindo cartilagem hialina com pleomorfismo ocasional e células binucleadas. Foi diagnosticado condrossarcoma intraocular primário.(AU)

Sperm cryopreservation of freshwater fish bocachico ( Prochilodus magdalenae ) in DMSO and glucose and its effects on fertilization and hatching efficiency

Internal cryoprotectants (dimethyl sulfoxide - DMSO), as well as exter nal ones (glucose) have been of great importance for sperm cryopreservation in freshwater fish. The aim of this study was to evaluate both the fertilization and hatching rates of eggs fertilized with bocachico ( Prochilodus magdalenae ) spermatozoa cryoprese rved in different combinations of DMSO and glucose. Nine treatments were evaluated by a combination of three concentrations of DMSO: 5% (701 mM), 10% (1402 mM), 15% (v /v ; 2103 mM) and three concentr ations of glucose: 5.5% (305 mM), 6% (333 mM), 6.5% (w /v ; 361 mM) . Semen from males obtained by abdominal stripping 6 h after hormonal induction with carp pituitary extract was submitted to each treatment. The semen was frozen in 0.5 m l straws in a nitrogen vapor dry shipper for 30 m in and then in liquid nitrogen ( - 1 96°C). Five days later they were placed in water with a temperature of 60 °C for 8 sec and analyzed. A high total motility (71 .0 ± 7.0%) was observed when DMSO concentration was 10% and glucose was 6%, and a high linearity displacement (62.8 ± 6. 3 %) was observed when DMSO concentration was 5% and glucose was 5.5%. In conclusion, we found that for the purposes of cryopreservation of bocachico spermatozoa, the combination s of 10% DMSO + 5.5 or 6% glucose and 5% DMSO + 5.5 or 6% glucose produced the best re sults in terms of fertilization and hatching rates. This becomes the first report to successfully demonstrate the fertilizing capacity and larvae obtaining capabilities of cryopreserved bocachico semen.

Sperm cryopreservation of freshwater fish bocachico ( Prochilodus magdalenae ) in DMSO and glucose and its effects on fertilization and hatching efficiency

Internal cryoprotectants (dimethyl sulfoxide - DMSO), as well as exter nal ones (glucose) have been of great importance for sperm cryopreservation in freshwater fish. The aim of this study was to evaluate both the fertilization and hatching rates of eggs fertilized with bocachico ( Prochilodus magdalenae ) spermatozoa cryoprese rved in different combinations of DMSO and glucose. Nine treatments were evaluated by a combination of three concentrations of DMSO: 5% (701 mM), 10% (1402 mM), 15% (v /v ; 2103 mM) and three concentr ations of glucose: 5.5% (305 mM), 6% (333 mM), 6.5% (w /v ; 361 mM) . Semen from males obtained by abdominal stripping 6 h after hormonal induction with carp pituitary extract was submitted to each treatment. The semen was frozen in 0.5 m l straws in a nitrogen vapor dry shipper for 30 m in and then in liquid nitrogen ( - 1 96°C). Five days later they were placed in water with a temperature of 60 °C for 8 sec and analyzed. A high total motility (71 .0 ± 7.0%) was observed when DMSO concentration was 10% and glucose was 6%, and a high linearity displacement (62.8 ± 6. 3 %) was observed when DMSO concentration was 5% and glucose was 5.5%. In conclusion, we found that for the purposes of cryopreservation of bocachico spermatozoa, the combination s of 10% DMSO + 5.5 or 6% glucose and 5% DMSO + 5.5 or 6% glucose produced the best re sults in terms of fertilization and hatching rates. This becomes the first report to successfully demonstrate the fertilizing capacity and larvae obtaining capabilities of cryopreserved bocachico semen.(AU)

Síndrome úveo-dermatológica do Akita com comprometimento neurológico. Relato de 4 casos

O artigo não apresenta resumo.

Síndrome úveo-dermatológica do Akita com comprometimento neurológico. Relato de 4 casos

O artigo não apresenta resumo.