Estimation of carbon stock in the soil plant system of the main phytophysiognomies of the cerrado of west Bahia, Brazil / Estimativa do estoque de carbono no sistema solo planta das principais fitofisionomias do cerrado do oeste da Bahia, Brasil

The work was carried out in the western region of Bahia in five phytophysiognomies of cerrado. The regional climate is of the Aw type with rainy summers and dry winters. The main soils are Red-Yellow Latosol, Quartzarenic Neosol and Dystrophic Haplic Gleysol with texture varying from sandy to sandy loam. Soil samples were taken up to 0.40 m to determine the soil carbon and for plant biomass models already established by other authors were chosen. The total soil carbon content followed the decreasing order: VRD>CRD>CSS>CCS>CCL. This may reflect the difference in density of plants in these phytophysiognomic compartments, although there is no significant difference between the soils under the phytophysiognomies of CCS and CCL. The soils under the five phytophysiognomies, on average, presented a carbon stock of 50.63 Mg ha-1. The studied phytophysiognomies in aboveground vegetation presented carbon stocks ranging from 0.05 to 23.36 Mg ha-1.This variation is explained by the plant diversity of each phytophysiognomy. Therefore, the accumulation of carbon depends on the richness and size of species of the area. In general, the upper soil layers stored greater amounts of carbon. Above ground biomass and in roots stand out for higher carbon production in biomass, the phytophysiognomies of CRD and CSS.

O trabalho foi desenvolvido na região Oeste da Bahia em cinco fitofisionomias de cerrado. O clima regional é do tipo Aw, com verão chuvoso e invernos seco. Os principais solos são Latossolo Vermelho-Amarelo e Neossolo Quartzarênicos, com textura variando de arenosa a franco arenosa. As amostras de solo foram retiradas até 0,40 m, para determinar o carbono do solo, para a biomassa vegetal, foram escolhidos modelos já consagrados por outros autores. O teor carbono total do solo obedeceu a seguinte ordem decrescente: VRD>CRD>CSS>CCS>CCL, fato que pode refletir a diferença de adensamento de plantas nesses compartimentos fitofisionômicos, apesar da não haver diferença significativa entre os solos sob as fitofisionomias de CCS e CCL. Em média, os solos sob as cinco fitofisionomias apresentaram média de estoque de carbono de 50,63 Mg ha-1. As fitofisionomias estudas, na vegetação acima do solo, apresentaram estoque de carbono variando 0,05 a 23,36 Mg ha-1. Essa variação é explicada pela diversidade vegetal de cada fitofisionomia. Portanto, o acumulo de carbono depende da riqueza e porte das espécies da área. De modo geral, as camadas superficiais do solo armazenaram maiores quantidades de carbono. A biomassa acima do solo e nas raízes, destacam-se por maiores produção de carbono na biomassa, as fitofisionomias de CRD e CSS.

Forage performance and cattle production as a function of the seasonality of a Brazilian tropical region

This study aimed to evaluate forage yield, tiller population density, canopy height, nutritional value as well as cattle performance in alternate stocking from three tropical forage cultivars. The experiment followed a randomized block design, in a 3x3 factorial scheme with three replications. Three forage cultivars (Marandu, Massai and Xaraés) distributed in nine hectares were evaluated in three dry season periods (transition wet/dry, dry,and end of dry season), with repeated measuresover time. The following variables were evaluated: canopyheight, leaf: stem ratio, forage mass, leaf mass, stem mass and dead material mass. Total digestible nutrients, crude protein and in vitrodry mass digestibility were evaluated regarding forage nutrition. Animal performance, using 36 eleven-month-old animals (half-blood Nelore),was evaluated by the daily average gain and weight gain by area. During pre-grazing, canopy height presented interaction among forage cultivars and the evaluated periods. The average daily gain showed no interaction amongcultivarsand period of the year the similarity in animal performance amongthe cultivars Marandu and Massai show that the Massai grass can be used as an alternative for forage diversification under an alternate management system.(AU)

Forage performance and cattle production as a function of the seasonality of a Brazilian tropical region

This study aimed to evaluate forage yield, tiller population density, canopy height, nutritional value as well as cattle performance in alternate stocking from three tropical forage cultivars. The experiment followed a randomized block design, in a 3 x 3 factorial scheme with three replications. Three forage cultivars (Marandu, Massai and Xaraés) distributed in nine hectares were evaluated in three dry season periods (transition wet/dry, dry, and end of dry season), with repeated measures over time. The following variables were evaluated: canopy height, leaf: stem ratio, forage mass, leaf mass, stem mass and dead material mass. Total digestible nutrients, crude protein and in vitro dry mass digestibility were evaluated regarding forage nutrition. Animal performance, using 36 eleven-month-old animals (half-blood Nelore), was evaluated by the daily average gain and weight gain by area. During pre-grazing, canopy height presented interaction among forage cultivars and the evaluated periods. The average daily gain showed no interaction among cultivars and period of the year the similarity in animal performance among the cultivars Marandu and Massai show that the Massai grass can be used as an alternative for forage diversification under an alternate management system.(AU)

Forage performance and cattle production as a function of the seasonality of a Brazilian tropical region / Forage performance and cattle production as a function of the seasonality of a Brazilian tropical region

This study aimed to evaluate forage yield, tiller population density, canopy height, nutritional value as well as cattle performance in alternate stocking from three tropical forage cultivars. The experiment followed a randomized block design, in a 3 x 3 factorial scheme with three replications. Three forage cultivars (Marandu, Massai and Xaraés) distributed in nine hectares were evaluated in three dry season periods (transition wet/dry, dry, and end of dry season), with repeated measures over time. The following variables were evaluated: canopy height, leaf: stem ratio, forage mass, leaf mass, stem mass and dead material mass. Total digestible nutrients, crude protein and in vitro dry mass digestibility were evaluated regarding forage nutrition. Animal performance, using 36 eleven-month-old animals (half-blood Nelore), was evaluated by the daily average gain and weight gain by area. During pre-grazing, canopy height presented interaction among forage cultivars and the evaluated periods. The average daily gain showed no interaction among cultivars and period of the year the similarity in animal performance among the cultivars Marandu and Massai show that the Massai grass can be used as an alternative for forage diversification under an alternate management system.

This study aimed to evaluate forage yield, tiller population density, canopy height, nutritional value as well as cattle performance in alternate stocking from three tropical forage cultivars. The experiment followed a randomized block design, in a 3 x 3 factorial scheme with three replications. Three forage cultivars (Marandu, Massai and Xaraés) distributed in nine hectares were evaluated in three dry season periods (transition wet/dry, dry, and end of dry season), with repeated measures over time. The following variables were evaluated: canopy height, leaf: stem ratio, forage mass, leaf mass, stem mass and dead material mass. Total digestible nutrients, crude protein and in vitro dry mass digestibility were evaluated regarding forage nutrition. Animal performance, using 36 eleven-month-old animals (half-blood Nelore), was evaluated by the daily average gain and weight gain by area. During pre-grazing, canopy height presented interaction among forage cultivars and the evaluated periods. The average daily gain showed no interaction among cultivars and period of the year the similarity in animal performance among the cultivars Marandu and Massai show that the Massai grass can be used as an alternative for forage diversification under an alternate management system.

Tracheal post-vaccinal reaction to different strains of Newcastle disease vírus / Reação traqueal pós-vacinal à diferentes cepas do vírus da doença de Newcastle

The aim of the present study was to evaluate the post-vaccinal reaction to two lentogenic vaccine strains of Newcatle disease virus (NDV) and a recombinant turkey herpesvirus (rHVT) vaccine expressing the fusion glycoprotein of NDV in broiler chickens through histomorphometric and histopathologic analyses of the trachea. The experiment involved 245 chicks housed in randomized blocks with three different enclosures under controlled conditions of temperature, light and ventilation. Each enclosure represented a vaccine strain and was divided into groups according to the administration route. Each block also had its own control group composed of unvaccinated birds. The vaccine strains PHY.LMV.42 (PL42) and La Sota (LS) were selected according to the Intracerebral Pathogenicity Index (ICPI) and the rHVT-NDV Serotype 3 strain (ST3) was selected for representing non-NDV infection. At two, four, seven, 14 and 21 days post vaccination, fragments from the middle third of the trachea were collected and submitted to routine histological processing. For the histomorphometric analysis, the slides were photographed, and the thickness of the tracheal mucosa was measured. Statistical analysis involved two-way ANOVA and Tukey's post-hoc test with a 5% significance level. For the histopathological evaluation, lesions were described as to the degree of intensity and distribution. At four and 14 days post vaccination with the LS strain administered by the ocular route, the means of thickening of the tracheal mucosa (20.85±7.31µm and 26.97±5.50µm, respectively) were significantly higher (p<0.05) than for all other strains, which was related to the severe histopathological lesions found in this group, characterized by hyperemia, hyperplasia of the mucous glands, moderate deciliation and multifocal lymphohistiocytic inflammatory infiltrate. At 21 days, broiler chickens vaccinated with the ST3 strain showed more discrete lesions and less thickening of the tracheal mucosa (23.23±7.62µm; p<0.05) in comparison with other studied strains. The lesions found in this group were only hemorrhage, deciliation and mild focal lymphocytic inflammatory infiltrate. The results of the histomorphometry and histopathology of the trachea indicated that vaccination with rHVT-NDV Serotype 3 strain induced lower degree post-vaccine tracheal lesions compared to other vaccine strains analyzed in this study.

Objetivou-se avaliar a reação pós-vacinal de duas estirpes lentogênicas do vírus da doença de Newcastle (VDN) e uma vacina recombinante de herpesvirus de perus (rHVT) que expressa a glicoproteína de fusão de VDN em frangos de corte por meio da histomorfometria e histopatologia da traqueia. Foram utilizados 245 pintos alojados em blocos ao acaso, sendo três galpões distintos em condições controladas de temperatura, luz e ventilação. Cada galpão representou uma cepa vacinal, onde foram divididos por grupos de acordo com a via de administração. Todos os blocos possuíam um grupo controle composto por aves não vacinadas. As cepas vacinais PHY.LMV.42 (PL42) e La Sota (LS) utilizadas foram selecionadas de acordo com o Índice de Patogenicidade Intracerebral (IPIC) e a cepa Sorotipo 3 (ST3), da vacina rHVT-VDN foi selecionada por não representar infecção do VDN. Aos dois, quarto, sete, 14 e 21 dias pós-vacinação, fragmentos do terço médio da traqueia foram coletados e posteriormente processados conforme rotina histológica. Para análise histomorfométrica da mucosa traqueal, as lâminas foram fotografadas e realizadas as mensurações da espessura da mucosa traqueal sendo aplicado teste de análise de variância a dois critérios (ANOVA) e utilizando o post-hoc de Tukey com nível de significância de 5%. Para a avaliação histopatológica foram observadas a presença de lesões microscópicas e estas foram descritas quanto ao grau de intensidade e distribuição. Aos quatro e quatorze dias pós-vacinação com a cepa LS administrada por via ocular, as médias do espessamento da mucosa traqueal (20,85±7,31µm e 26,97±5,50µm, respectivamente) foram significativamente maiores (p<0,05) quando comparada a todas as demais cepas utilizadas, isto se deve às severas lesões histopatológicas encontrados neste grupo, caracterizadas por hiperemia, hiperplasia das glândulas mucosas, deciliação moderada e infiltrado inflamatório linfohistiocitário multifocal moderado. Já aos 21 dias as aves vacinadas com a cepa ST3 apresentaram lesões mais discretas e menor espessamento da mucosa da traqueia (23,23±7,62µm; p<0,05) em comparação às demais cepas estudadas. As lesões encontradas neste grupo foram apenas hemorragia, deciliação e infiltrado inflamatório linfocitário focal discreto. Os resultados da histomorfometria e da histopatologia da traqueia indicou que a vacinação com a rHVT-NDV, cepa Sorotipo 3 induziu menor grau de lesões pós-vacinais na traqueia comparada a outras cepas vacinais analisadas nesse estudo.

Copper deficiency in dairy goats and kids / Deficiência de cobre em cabras leiteiras e cabritos

The clinical, pathological and reproductive aspects of an outbreak of copper deficiency in dairy goats and kids from the semiarid region of Pernambuco, Brazil are described. Ten adult dairy goats with clinical signs of deficiency and four kids presenting enzootic ataxia born from copper deficient does were separated from the herd, and examined. In the dairy goats, the average serum concentration of copper was 6.1±2.8mmol/L and iron was 39.5±8.2mmol/L. In kids, the average serum concentration of copper was 3.8±0.9mmol/L and iron was 38.5±4.1mmol/L. Clinical signs in dairy goats consisted of pale mucous membranes, anemia, emaciation, diarrhea, achromotrichia, brittle hair and alopecia. The main reproductive alterations consisted of prolonged anestrus, embryonic resorption and high indices of retained placenta. The kids born from copper deficient dairy goats were weak, and presented neonatal or late ataxia until 70 days of life. Six dairy goats and four kids were necropsied. Most ovaries examined were small, firm and did not present viable follicles on their surface. Microscopically, there was reduction of viable follicles in addition to disorganization of follicular and stromal structures, with marked follicular atresia. Microscopically, changes in kids with enzootic ataxia consisted of neuronal chromatolysis and axonal degeneration, mainly in neurons of the spinal cord. In this study, the source of high iron was not identified, but it is known that outbreaks of copper deficiency can occur due to excess iron intake, mainly when adequate mineral supplementation is not provided for the goat herds.

Descrevem-se os aspectos clínicos, patológicos e reprodutivos de um surto de deficiência de cobre em cabras leiteiras e em cabritos da região semiárida de Pernambuco, Brasil. Dez cabras leiteiras adultas com sinais clínicos de deficiência de cobre e quatro cabritos com ataxia enzoótica nascidos dessas cabras foram separados do rebanho e examinados. Nas cabras leiteiras, a concentração média de cobre sérico foi de 6,1±2,8mmol/L e a concentração média de ferro sérico foi de 39,5±8,2mmol/L. Nos cabritos, a concentração média de cobre sérico foi de 3,8±0,9mmol/L e a concentração média de ferro sérico foi de 38,5±4,1mmol/L. Os sinais clínicos nas cabras consistiram em mucosas pálidas, anemia, diarreia, acromotriquia, pelos opacos e quebradiços e alopecia. As principais alterações reprodutivas consistiram em anestro prolongado, reabsorção embrionária e aumento da taxa de retenção de placenta. Os cabritos nascidos vivos dessas cabras nasceram fracos, com ataxia neonatal ou desenvolveram ataxia tardia em até 70 dias de vida. Seis cabras leiteiras e quatro cabritos foram necropsiados. Nas cabras, a maioria dos ovários examinados macroscopicamente eram pequenos, firmes e não apresentavam folículos viáveis em sua superfície. Microscopicamente, houve redução de folículos viáveis, além de desorganização das estruturas foliculares, com marcada atresia folicular. Macroscopicamente não foram observadas alterações nos cabritos com ataxia enzoótica. As lesões histológicas observadas nesses cabritos consistiram em cromatólise neuronal e degeneração axonal, principalmente em neurônios da medula espinhal. Neste estudo a fonte dos altos teores de ferro no soro não foi identificada, mas sabe-se que surtos de deficiência de cobre podem ocorrer devido ao excesso de ingestão de ferro, principalmente quando não é fornecida suplementação mineral adequada para os rebanhos caprinos.

Effects of the healing activity of rosemary-of-Chapada (Lippia gracilis Schauer) on cutaneous lesions in rats

Purpose: To evaluate the effects of rosemary leaf essential oil-based ointments on the healing of rat skin lesions. Methods: Sixty adult male rats, with dorsal excisional skin wounds made surgically under anesthesia, were divided into three groups (n = 20): Sham group (untreated wounds); control group (CG, wounds treated with vehicle); and essential oil (EO) treated group (wounds treated with essential oil-based ointments), administered topically once daily. Skin wounds were evaluated at 4, 7, 14, and 21 days after EO or vehicle treatments. Lesions were analyzed macroscopically for the contraction degree. Formalin-fixed paraffin-embedded sections of skin wounds were used for histopathological evaluation. Results: Macroscopic evaluation showed wounds edges with thin crust without firmness and yellowish color, along with an improvement in wound contraction in EO group when compared to the other groups. A reduced inflammatory reaction, along with newly formed small diameter capillaries and more organized and elongated collagen fibers, were more frequently observed in EO group than in the other groups. Moreover, blood vessel number and collagen fibers density were significantly higher in EO group. Conclusions: Skin lesion treatment with rosemary leaf essential oil-based ointments accelerates the initial stages of healing, reduces inflammation, and increases angiogenesis, collagen fibers density, and wound contraction in rats.

Equine coital exanthema caused by equid alphaherpesvirus 3: a report of an outbreak in northeastern Brazil

ABSTRACT: Equine coital exanthema is a venereal infectious disease poorly reported in horses in Brazil and was never described in the northeastern region of the country. This work aims to describe the clinical and pathological aspects of an outbreak of equine coital exanthema caused by equid alphaherpesvirus 3, occurred in a herd of horses at the semiarid region of the State of Rio Grande do Norte. Main clinical signs consisted of anorexia, hiporexia, fibrinous or purulent secretion in the penis mucosa and vagina. Two mares presented mild to minimal lesions that consisted of scars in the mucosa of the vagina and in the perivulvar region. In a stallion the disease consisted of severe, multifocal, umbilicated-exanthematous ulcers of approximately 1cm in diameter on the penis mucosa. Other areas where ulcers and crusts were focally observed included the skin of the scrotum and on the lips and mucocutaneous junctions of the oral cavity. Histologically, the main lesion consisted of multifocal severe ulcerative and fibrinous necrotizing balanoposthitis and mild multifocal necrotizing, lymphocytic dermatitis in the lips and scrotum. The equide alphaherpesvirus 3 DNA was amplified in blood samples and penis mucosa using the PCR technique. This is the first report of molecular diagnosis of equine coital exanthema affecting horses in northeastern Brazil. Further studies should be carried out in order to investigate the epidemiology and the importance of this herpetic disease in the country.

RESUMO: O exantema coital equino é uma doença infecciosa venérea pouco relatada em equinos no Brasil e nunca descrita na região Nordeste do país. Este trabalho tem como objetivo descrever os aspectos clínicos e patológicos de um surto de exantema coital equino causado pelo alphaherpesvirus equídeo 3, que ocorreu em um haras na região semiárida do Estado do Rio Grande do Norte. Os principais sinais clínicos consistiram em anorexia, hiporexia, secreção fibrinosa ou purulenta na mucosa do pênis e vagina. Duas éguas apresentavam lesões discretas que consistiam em cicatrizes na mucosa da vagina e na região perivulvar. Em um garanhão, a doença consistia em úlceras umbilicadas-exantematosas severas, multifocais, de aproximadamente 1 cm de diâmetro na mucosa do pênis. Outras áreas onde úlceras e crostas foram observadas focalmente incluíram a pele do escroto, lábios e junções mucocutâneas da cavidade oral. Histologicamente, as principais lesões consistiam em balanopostite multifocal ulcerativa e necrosante fibrinosa grave e dermatite linfocítica necrosante multifocal leve nos lábios e escroto. O DNA do alphaherpesvirus equídeo tipo 3 foi amplificado em amostras de sangue e mucosa do pênis pela técnica de PCR. Este é o primeiro relato de diagnóstico molecular de exantema coital equino afetando cavalos no nordeste do Brasil. Novos estudos devem ser realizados a fim de investigar a epidemiologia e a importância dessa doença herpética no país.

Equine coital exanthema caused by equid alphaherpesvirus 3: a report of an outbreak in northeastern Brazil / Exantema coital equino causado pelo alphaherpesvirus equídeo tipo 3: relato de um surto no nordeste do Brasil

Equine coital exanthema is a venereal infectious disease poorly reported in horses in Brazil and was never described in the northeastern region of the country. This work aims to describe the clinical and pathological aspects of an outbreak of equine coital exanthema caused by equid alphaherpesvirus 3, occurred in a herd of horses at the semiarid region of the State of Rio Grande do Norte. Main clinical signs consisted of anorexia, hiporexia, fibrinous or purulent secretion in the penis mucosa and vagina. Two mares presented mild to minimal lesions that consisted of scars in the mucosa of the vagina and in the perivulvar region. In a stallion the disease consisted of severe, multifocal, umbilicated-exanthematous ulcers of approximately 1cm in diameter on the penis mucosa. Other areas where ulcers and crusts were focally observed included the skin of the scrotum and on the lips and mucocutaneous junctions of the oral cavity. Histologically, the main lesion consisted of multifocal severe ulcerative and fibrinous necrotizing balanoposthitis and mild multifocal necrotizing, lymphocytic dermatitis in the lips and scrotum. The equide alphaherpesvirus 3 DNA was amplified in blood samples and penis mucosa using the PCR technique. This is the first report of molecular diagnosis of equine coital exanthema affecting horses in northeastern Brazil. Further studies should be carried out in order to investigate the epidemiology and the importance of this herpetic disease in the country.(AU)

O exantema coital equino é uma doença infecciosa venérea pouco relatada em equinos no Brasil e nunca descrita na região Nordeste do país. Este trabalho tem como objetivo descrever os aspectos clínicos e patológicos de um surto de exantema coital equino causado pelo alphaherpesvirus equídeo 3, que ocorreu em um haras na região semiárida do Estado do Rio Grande do Norte. Os principais sinais clínicos consistiram em anorexia, hiporexia, secreção fibrinosa ou purulenta na mucosa do pênis e vagina. Duas éguas apresentavam lesões discretas que consistiam em cicatrizes na mucosa da vagina e na região perivulvar. Em um garanhão, a doença consistia em úlceras umbilicadas-exantematosas severas, multifocais, de aproximadamente 1 cm de diâmetro na mucosa do pênis. Outras áreas onde úlceras e crostas foram observadas focalmente incluíram a pele do escroto, lábios e junções mucocutâneas da cavidade oral. Histologicamente, as principais lesões consistiam em balanopostite multifocal ulcerativa e necrosante fibrinosa grave e dermatite linfocítica necrosante multifocal leve nos lábios e escroto. O DNA do alphaherpesvirus equídeo tipo 3 foi amplificado em amostras de sangue e mucosa do pênis pela técnica de PCR. Este é o primeiro relato de diagnóstico molecular de exantema coital equino afetando cavalos no nordeste do Brasil. Novos estudos devem ser realizados a fim de investigar a epidemiologia e a importância dessa doença herpética no país.(AU)

Equine coital exanthema caused by equid alphaherpesvirus 3: a report of an outbreak in northeastern Brazil / Exantema coital equino causado pelo alphaherpesvirus equídeo tipo 3: relato de um surto no nordeste do Brasil

Equine coital exanthema is a venereal infectious disease poorly reported in horses in Brazil and was never described in the northeastern region of the country. This work aims to describe the clinical and pathological aspects of an outbreak of equine coital exanthema caused by equid alphaherpesvirus 3, occurred in a herd of horses at the semiarid region of the State of Rio Grande do Norte. Main clinical signs consisted of anorexia, hiporexia, fibrinous or purulent secretion in the penis mucosa and vagina. Two mares presented mild to minimal lesions that consisted of scars in the mucosa of the vagina and in the perivulvar region. In a stallion the disease consisted of severe, multifocal, umbilicated-exanthematous ulcers of approximately 1cm in diameter on the penis mucosa. Other areas where ulcers and crusts were focally observed included the skin of the scrotum and on the lips and mucocutaneous junctions of the oral cavity. Histologically, the main lesion consisted of multifocal severe ulcerative and fibrinous necrotizing balanoposthitis and mild multifocal necrotizing, lymphocytic dermatitis in the lips and scrotum. The equide alphaherpesvirus 3 DNA was amplified in blood samples and penis mucosa using the PCR technique. This is the first report of molecular diagnosis of equine coital exanthema affecting horses in northeastern Brazil. Further studies should be carried out in order to investigate the epidemiology and the importance of this herpetic disease in the country.(AU)

O exantema coital equino é uma doença infecciosa venérea pouco relatada em equinos no Brasil e nunca descrita na região Nordeste do país. Este trabalho tem como objetivo descrever os aspectos clínicos e patológicos de um surto de exantema coital equino causado pelo alphaherpesvirus equídeo 3, que ocorreu em um haras na região semiárida do Estado do Rio Grande do Norte. Os principais sinais clínicos consistiram em anorexia, hiporexia, secreção fibrinosa ou purulenta na mucosa do pênis e vagina. Duas éguas apresentavam lesões discretas que consistiam em cicatrizes na mucosa da vagina e na região perivulvar. Em um garanhão, a doença consistia em úlceras umbilicadas-exantematosas severas, multifocais, de aproximadamente 1 cm de diâmetro na mucosa do pênis. Outras áreas onde úlceras e crostas foram observadas focalmente incluíram a pele do escroto, lábios e junções mucocutâneas da cavidade oral. Histologicamente, as principais lesões consistiam em balanopostite multifocal ulcerativa e necrosante fibrinosa grave e dermatite linfocítica necrosante multifocal leve nos lábios e escroto. O DNA do alphaherpesvirus equídeo tipo 3 foi amplificado em amostras de sangue e mucosa do pênis pela técnica de PCR. Este é o primeiro relato de diagnóstico molecular de exantema coital equino afetando cavalos no nordeste do Brasil. Novos estudos devem ser realizados a fim de investigar a epidemiologia e a importância dessa doença herpética no país.(AU)

Use of endometrial vascular perfusion by color Doppler, B-mode ultrasonography and cytology for diagnosis of subclinical endometritis in Holstein cows / Uso da perfusão vascular endometrial por Doppler colorido, ultrassonografia modo B e citologia no diagnóstico de endometrite subclínica em vacas da raça Holandesa

The aim was to evaluate the use of ultrasonography color Doppler mode for diagnosis the subclinical endometritis (SE) in dairy cows. Lactating Holstein cows on a semi intensive system were evaluated (n= 152) and distributed into two groups (C1 and C2). The C1 (n = 60) was composed of primiparous and the C2 (n = 92) by pluriparous. All females (C1 and C2) were submitted gynaecological examination by transrectal palpation and evaluation with the Metricheck device (MD) and endometrial cytology (EC) to determine the percentage of neutrophils (PMNs). Ultrasonographic examinations were performed in the B mode for the presence of echogenic intrauterine fluid (IUF-e) and for color Doppler mode endometrial vascular perfusion evaluation (ENDOVP). Data were submitted to ANOVA with Pearson correlation and chi-square test at 5%. In the evaluation with MD, 16.7% of the cows in the C1 and 17.4% in the C2 were considered inapt. Regarding SE prevalence was 24.0% (C1) and 44.7% (C2). As far as the ultrasound evaluation of the IUF-e was present in 68.0% of the cows of the C1 and in 75.0% in the C2. In the ENDOVP, scores 1 (38.0%), 2 (36.5%), 3 (23.0%) and 4 (8.7%) were evidenced. There was a correlation between ENDOVP and PMN in C1 (r = 0.8336) and in C2 (r = 0.7196). Therefore, it is concluded that ENDOVP proved to be efficient for the diagnosis of SE, in view of its strong correlation with PMN.(AU)

O objetivo foi avaliar o uso da ultrassonografia no modo Doppler colorido para o diagnóstico da endometrite subclínica (ES) em vacas leiteiras. Vacas da raça Holandesa foram avaliadas (n = 152) em lactação e mantidas em sistema semi-intensivo. Elas foram distribuídas em dois grupos (C1 e C2). O C1 (n = 60) foi composto por primíparas e o C2 (n = 92) por pluríparas. Todas as vacas (C1 e C2) foram submetidas a exame ginecológico por palpação transretal e avaliação com o dispositivo Metricheck (DM). Depois, amostras foram coletadas para citologia endometrial (CE) para determinar a porcentagem de neutrófilos (PMNs). Os exames ultrassonográficos foram realizados no modo B para a presença de líquido intra-uterino ecogênico (IUF-e) e para avaliação da perfusão vascular endometrial no modo Doppler colorido (ENDOVP). Os dados foram submetidos à ANOVA com correlação de Pearson e teste do qui-quadrado a 5%. Na avaliação com DM, 16,7% das vacas no C1 e 17,4% no C2 foram consideradas inaptas. Em relação a prevalência de ES, foi de 24,0% (C1) e 44,7% (C2). Quanto à avaliação ultrassonográfica do IUF-e, esteve presente em 68,0% das vacas do C1 e em 75,0% no C2. Na avaliação da ENDOVP, foram evidenciados os escores 1 (38,0%), 2 (36,5%), 3 (23,0%) e 4 (8,7%). Houve correlação entre ENDOVP e PMN no C1 (r = 0,8336) e no C2 (r = 0,7196). Portanto, conclui-se que a ENDOVP se mostrou eficiente para o diagnóstico de ES, tendo em vista sua forte correlação com os PMN.(AU)

Use of endometrial vascular perfusion by color Doppler, B-mode ultrasonography and cytology for diagnosis of subclinical endometritis in Holstein cows / Uso da perfusão vascular endometrial por Doppler colorido, ultrassonografia modo B e citologia no diagnóstico de endometrite subclínica em vacas da raça Holandesa

The aim was to evaluate the use of ultrasonography color Doppler mode for diagnosis the subclinical endometritis (SE) in dairy cows. Lactating Holstein cows on a semi intensive system were evaluated (n= 152) and distributed into two groups (C1 and C2). The C1 (n = 60) was composed of primiparous and the C2 (n = 92) by pluriparous. All females (C1 and C2) were submitted gynaecological examination by transrectal palpation and evaluation with the Metricheck device (MD) and endometrial cytology (EC) to determine the percentage of neutrophils (PMNs). Ultrasonographic examinations were performed in the B mode for the presence of echogenic intrauterine fluid (IUF-e) and for color Doppler mode endometrial vascular perfusion evaluation (ENDOVP). Data were submitted to ANOVA with Pearson correlation and chi-square test at 5%. In the evaluation with MD, 16.7% of the cows in the C1 and 17.4% in the C2 were considered inapt. Regarding SE prevalence was 24.0% (C1) and 44.7% (C2). As far as the ultrasound evaluation of the IUF-e was present in 68.0% of the cows of the C1 and in 75.0% in the C2. In the ENDOVP, scores 1 (38.0%), 2 (36.5%), 3 (23.0%) and 4 (8.7%) were evidenced. There was a correlation between ENDOVP and PMN in C1 (r = 0.8336) and in C2 (r = 0.7196). Therefore, it is concluded that ENDOVP proved to be efficient for the diagnosis of SE, in view of its strong correlation with PMN.

O objetivo foi avaliar o uso da ultrassonografia no modo Doppler colorido para o diagnóstico da endometrite subclínica (ES) em vacas leiteiras. Vacas da raça Holandesa foram avaliadas (n = 152) em lactação e mantidas em sistema semi-intensivo. Elas foram distribuídas em dois grupos (C1 e C2). O C1 (n = 60) foi composto por primíparas e o C2 (n = 92) por pluríparas. Todas as vacas (C1 e C2) foram submetidas a exame ginecológico por palpação transretal e avaliação com o dispositivo Metricheck (DM). Depois, amostras foram coletadas para citologia endometrial (CE) para determinar a porcentagem de neutrófilos (PMNs). Os exames ultrassonográficos foram realizados no modo B para a presença de líquido intra-uterino ecogênico (IUF-e) e para avaliação da perfusão vascular endometrial no modo Doppler colorido (ENDOVP). Os dados foram submetidos à ANOVA com correlação de Pearson e teste do qui-quadrado a 5%. Na avaliação com DM, 16,7% das vacas no C1 e 17,4% no C2 foram consideradas inaptas. Em relação a prevalência de ES, foi de 24,0% (C1) e 44,7% (C2). Quanto à avaliação ultrassonográfica do IUF-e, esteve presente em 68,0% das vacas do C1 e em 75,0% no C2. Na avaliação da ENDOVP, foram evidenciados os escores 1 (38,0%), 2 (36,5%), 3 (23,0%) e 4 (8,7%). Houve correlação entre ENDOVP e PMN no C1 (r = 0,8336) e no C2 (r = 0,7196). Portanto, conclui-se que a ENDOVP se mostrou eficiente para o diagnóstico de ES, tendo em vista sua forte correlação com os PMN.

Evaluation of platelet-rich plasma gel as an angiogenesis-inducing agent in canine advancement skin flaps / Avaliação do gel de plasma rico em plaquetas como agente indutor da angiogênese em flapes cutâneos de avanço em cães

This work aimed to evaluate the effect of platelet-rich plasma (PRP) on advancement skin flaps in dogs regarding improvement of vascularization, with focus on increasing its viable area, since there are reports that it is a potential angiogenesis stimulator. The experimental group was composed of eight adult bitches, in which two advancement skin flaps were made in the ventral abdominal region. No product was applied in the control flap (CF), while PRP was used in the contralateral flap, called treated flap (TF). The areas were clinically evaluated every two days until the 7th postoperative day regarding skin color and presence of necrosis. At 10 days, both flaps were removed and submitted to histological examination and blood vessel morphometry. The vessels counted in each group were statistically analyzed by the F-test at 1% probability. Results showed no significant difference in macroscopic changes in the wound, or CF and TF vascularization, thus suggesting that PRP gel did not improve advancement skin flap angiogenesis in bitches under the experimental conditions in which this research was developed.(AU)

Objetivou-se com o presente artigo avaliar a ação angiogênica do gel de plasma rico em plaquetas (PRP) em flapes cutâneos de avanço em animais da espécie canina, visando aumentar a viabilidade da pele após o procedimento, uma vez que existem relatos de que o produto é um potente estimulador da angiogênese. O grupo experimental foi composto por oito cadelas adultas, onde foram confeccionados dois flapes de avanço (de padrão subdérmico) na região abdominal ventral. Em um dos flapes, considerado controle (FC) não foi aplicado nenhum produto, enquanto que no flape contralateral, denominado tratado (FT), foi usado o gel de PRP. As áreas foram macroscopicamente avaliadas a cada dois dias até o 7º dia de pós-operatório em relação à coloração da pele e presença de área de necrose, e com 10 dias ambos os flapes foram coletados por biópsia e submetidos ao exame histológico e morfometria dos vasos sanguíneos. Os vasos contados em cada grupo foram estatisticamente analisados pelo teste de F ao nível de 1% de probabilidades. Os resultados demonstraram que não houve diferença significativa nas alterações macroscópicas das feridas e na morfometria vascular dos FC e FT, sugerindo dessa maneira que dentro das condições experimentais nas quais a pesquisa foi executada, que o gel de PRP não incrementou a angiogênese de flapes de avanço em cadelas.(AU)

Evaluation of platelet-rich plasma gel as an angiogenesis-inducing agent in canine advancement skin flaps / Avaliação do gel de plasma rico em plaquetas como agente indutor da angiogênese em flapes cutâneos de avanço em cães

This work aimed to evaluate the effect of platelet-rich plasma (PRP) on advancement skin flaps in dogs regarding improvement of vascularization, with focus on increasing its viable area, since there are reports that it is a potential angiogenesis stimulator. The experimental group was composed of eight adult bitches, in which two advancement skin flaps were made in the ventral abdominal region. No product was applied in the control flap (CF), while PRP was used in the contralateral flap, called treated flap (TF). The areas were clinically evaluated every two days until the 7th postoperative day regarding skin color and presence of necrosis. At 10 days, both flaps were removed and submitted to histological examination and blood vessel morphometry. The vessels counted in each group were statistically analyzed by the F-test at 1% probability. Results showed no significant difference in macroscopic changes in the wound, or CF and TF vascularization, thus suggesting that PRP gel did not improve advancement skin flap angiogenesis in bitches under the experimental conditions in which this research was developed.(AU)

Objetivou-se com o presente artigo avaliar a ação angiogênica do gel de plasma rico em plaquetas (PRP) em flapes cutâneos de avanço em animais da espécie canina, visando aumentar a viabilidade da pele após o procedimento, uma vez que existem relatos de que o produto é um potente estimulador da angiogênese. O grupo experimental foi composto por oito cadelas adultas, onde foram confeccionados dois flapes de avanço (de padrão subdérmico) na região abdominal ventral. Em um dos flapes, considerado controle (FC) não foi aplicado nenhum produto, enquanto que no flape contralateral, denominado tratado (FT), foi usado o gel de PRP. As áreas foram macroscopicamente avaliadas a cada dois dias até o 7º dia de pós-operatório em relação à coloração da pele e presença de área de necrose, e com 10 dias ambos os flapes foram coletados por biópsia e submetidos ao exame histológico e morfometria dos vasos sanguíneos. Os vasos contados em cada grupo foram estatisticamente analisados pelo teste de F ao nível de 1% de probabilidades. Os resultados demonstraram que não houve diferença significativa nas alterações macroscópicas das feridas e na morfometria vascular dos FC e FT, sugerindo dessa maneira que dentro das condições experimentais nas quais a pesquisa foi executada, que o gel de PRP não incrementou a angiogênese de flapes de avanço em cadelas.(AU)

Effect of post-grazing residual leaf blade-length on the regrowth vigor of Piatã grass grazed under intermittent stocking / Efeito do comprimento residual de lâmina foliar pós-pastejo no vigor de rebrotação de capim Piatã pastejado sob lotação intermitente

The objective of this study was to evaluate the effect of post-grazing residual leaf blades (RLB) of different lengths on the morphogenic and structural characteristics of Urochloa brizantha 'Piatã' grazed upon by beef cattle. A completely randomized design was used, with three RLB lengths (2, 4, and 6 cm) and two paddocks as replicates over two grazing seasons (wet and wet-dry transition). To monitor the weekly forage growth, two representative clumps and 48 tillers per treatment were marked after grazing. The procedure continued until the plants reached the target pre-grazing height of 35-40 cm. Morphogenic characteristics were not affected by the treatments or seasons, with minor changes observed in mean sheath length (MSL) within season and tiller population density (TPD). The MSL reached its peak value of 210.37 mm during the wet season relative to 175.89 mm during the wet-dry transition. The TPD reached its lowest value (680 tillers m <->2) at the highest defoliation intensity (2 cm) relative to that at the lowest defoliation intensity (720 tillers m <->2). The different RLB lengths modified canopy height variably; canopy height was the highest with the 6 cm leaves (24.31 cm), but did not vary between the 2 cm and 4 cm leaves. The post-grazing leaf area index and interception of photosynthetically active radiation were modified by season. The highest defoliation intensity (2 cm) increased the rest period by seven days when compared with the lowest defoliation intensity (6 cm).(AU)

Objetivou-se avaliar o efeito de diferentes comprimentos de lâmina foliar residual (RLF) pós-pastejo sobre as respostas morfogênicas e estruturais do capim Urochloa brizantha cv. Piatã pastejado por bovinos de corte. O delineamento experimental utilizado foi o inteiramente casualizado, com três RLF (2, 4 e 6 cm) e duas repetições de piquetes, ao longo de duas estações (águas e transição águas-seca). Após o pastejo dos bovinos eram marcadas duas touceiras representativas, e 48 perfilhos por tratamento, para o acompanhamento semanal do crescimento forrageiro, durante o período necessário para as plantas atingirem as metas de alturas pré-pastejo determinada entre 35 e 40 cm. As características morfogênicas não diferenciaram entre os tratamentos e estações, que promoveu pouca alteração nas características estruturais, apenas comprimento médio de bainha (CMB) na estação e densidade populacional de perfilhos (DPP). O maior valor encontrado para CMB, foi na estação das águas 210,37 mm versus 175,89 mm para a transição. A DPP apresentou o menor valor (680 perfilhos m <->2) com a maior remoção (2 cm) em relação ao de menor remoção (720 perfilhos m <->2). As diferentes remoções de lâmina foliar proporcionou alteração na altura de saída, em que o tratamento 6 cm apresentou a maior altura (24,31 cm), não diferenciando os demais tratamentos. O índice de área foliar (IAF) e interceptação da radiação fotossinteticamente ativa (IRFA) foram modificados no pós pastejo. A maior remoção de lâmina foliar (2 cm) teve o seu período de descanso (PD) aumentado em 7 dias, em relação a menor remoção (6 cm).(AU)

Spontaneous poisoning by Ricinus communis leaves (Euphorbiaceae) in goats / Intoxicação espontânea por folhas de Ricinus communis (Euphorbiaceae) em caprinos

The aim of this study was to report the clinical and pathological aspects of an outbreak of poisoning by the ingestion of Ricinus communis leaves in a herd of goats at Pernambuco, northeastern Brazil. Within 3-5 hours after ingesting the sprouts and young shrubs of the plant, twenty Toggenburg female goats and two adults crossbred wethers presented acute neurological clinical signs, which were initially characterized by decreased locomotor activity that later evolved to severe ataxia, depression, incoordination and staggering gait. Four goat that died spontaneously were necropsied. Gross lesions were unspecific and consisted in focal areas of lungs edema, petechial hemorrhages in the epicardium and congestion and enlargement of liver. The contents of the rumen, reticulum and omasum were dry and contained leaves of the plant. Histologically there were no lesions in the CNS. In the liver, the main lesion consisted in cytoplasmic vacuolization and necrosis of hepatocytes. Eighteen goats recovered after a supportive therapy with activated charcoal, glycated isotonic solution, dexamethasone and vitamin B12. There is no specific therapy for poisoning by R. communis, however supportive and symptomatic treatments are recommended and should be based on the clinical signs.(AU)

O objetivo deste estudo foi relatar os aspectos clínicos e patológicos de um surto de intoxicação pelas folhas de Ricinus communis em um rebanho de caprinos em Pernambuco, Nordeste do Brasil. Três a cinco horas após a ingestão dos brotos e arbustos jovens da planta, vinte cabras da raça Toggenburg e dois machos mestiços apresentaram quadro clínico neurológico agudo caracterizado principalmente pela diminuição da atividade locomotora, grave ataxia, depressão, incoordenação e marcha cambaleante. Quatro caprinos morreram espontaneamente e foram necropsiados. Macroscopicamente, as lesões eram inespecíficas e consistiam em áreas focais de edema pulmonar, hemorragias petequiais epicárdicas e aumento do volume e congestão do fígado. Os conteúdos do rumem, retículo e omaso eram ressecados e continham folhas da planta. Histologicamente, não foram observadas lesões no SNC. No fígado, havia vacuolização citoplasmática e necrose de hepatócitos. Dezoito caprinos se recuperaram após receberem terapia de suporte com carvão ativado, soro glicosado, dexametasona e vitamina B12. Não existe terapêutica especifica para a intoxicação pelas folhas de R. Communis. Os tratamentos sintomáticos e de suporte são recomendados e devem basear-se nos sinais clínicos.(AU)

Spontaneous poisoning by Ricinus communis leaves (Euphorbiaceae) in goats / Intoxicação espontânea por folhas de Ricinus communis (Euphorbiaceae) em caprinos

The aim of this study was to report the clinical and pathological aspects of an outbreak of poisoning by the ingestion of Ricinus communis leaves in a herd of goats at Pernambuco, northeastern Brazil. Within 3-5 hours after ingesting the sprouts and young shrubs of the plant, twenty Toggenburg female goats and two adults crossbred wethers presented acute neurological clinical signs, which were initially characterized by decreased locomotor activity that later evolved to severe ataxia, depression, incoordination and staggering gait. Four goat that died spontaneously were necropsied. Gross lesions were unspecific and consisted in focal areas of lungs edema, petechial hemorrhages in the epicardium and congestion and enlargement of liver. The contents of the rumen, reticulum and omasum were dry and contained leaves of the plant. Histologically there were no lesions in the CNS. In the liver, the main lesion consisted in cytoplasmic vacuolization and necrosis of hepatocytes. Eighteen goats recovered after a supportive therapy with activated charcoal, glycated isotonic solution, dexamethasone and vitamin B12. There is no specific therapy for poisoning by R. communis, however supportive and symptomatic treatments are recommended and should be based on the clinical signs.(AU)

O objetivo deste estudo foi relatar os aspectos clínicos e patológicos de um surto de intoxicação pelas folhas de Ricinus communis em um rebanho de caprinos em Pernambuco, Nordeste do Brasil. Três a cinco horas após a ingestão dos brotos e arbustos jovens da planta, vinte cabras da raça Toggenburg e dois machos mestiços apresentaram quadro clínico neurológico agudo caracterizado principalmente pela diminuição da atividade locomotora, grave ataxia, depressão, incoordenação e marcha cambaleante. Quatro caprinos morreram espontaneamente e foram necropsiados. Macroscopicamente, as lesões eram inespecíficas e consistiam em áreas focais de edema pulmonar, hemorragias petequiais epicárdicas e aumento do volume e congestão do fígado. Os conteúdos do rumem, retículo e omaso eram ressecados e continham folhas da planta. Histologicamente, não foram observadas lesões no SNC. No fígado, havia vacuolização citoplasmática e necrose de hepatócitos. Dezoito caprinos se recuperaram após receberem terapia de suporte com carvão ativado, soro glicosado, dexametasona e vitamina B12. Não existe terapêutica especifica para a intoxicação pelas folhas de R. Communis. Os tratamentos sintomáticos e de suporte são recomendados e devem basear-se nos sinais clínicos.(AU)

Tremorgenic syndrome in suckling lambs due to poisoning by Ipomoea asarifolia

The aim of this study was to describe the clinical, pathological and epidemiological aspects of an outbreak of tremorgenic syndrome in suckling lambs associated to Ipomoea asarifolia poisoning occurred in the metropolitan region of Recife, northeastern Brazil. Eight suckling Dorper lambs presented clinical signs after their mothers being kept in pasture invaded by I. asarifolia. Sixty percent of adult sheep, including lactating ewe, only exhibited engorged episcleral vessels. The suckling lambs exhibited neurological signs, such as head and neck tremors, dysmetria, wide-based stance, uncoordinated gait, unusual standing and imbalance followed by falls. These signs became more evident when the animals were forced to move. No gross lesions were found in a necropsied lamb that had died spontaneously. The histological findings were axonal spheroids in the cerebellum granular layer and numerous necrotic neurons in the cerebral cortex and cerebellum molecular layer. This study demonstrates that Ipomoea asarifolia causes tremorgenic syndrome. The toxin is eliminated through milk and suckling lambs exhibit a greater susceptibility compared to adult animals.

Tremorgenic syndrome in suckling lambs due to poisoning by Ipomoea asarifolia

The aim of this study was to describe the clinical, pathological and epidemiological aspects of an outbreak of tremorgenic syndrome in suckling lambs associated to Ipomoea asarifolia poisoning occurred in the metropolitan region of Recife, northeastern Brazil. Eight suckling Dorper lambs presented clinical signs after their mothers being kept in pasture invaded by I. asarifolia. Sixty percent of adult sheep, including lactating ewe, only exhibited engorged episcleral vessels. The suckling lambs exhibited neurological signs, such as head and neck tremors, dysmetria, wide-based stance, uncoordinated gait, unusual standing and imbalance followed by falls. These signs became more evident when the animals were forced to move. No gross lesions were found in a necropsied lamb that had died spontaneously. The histological findings were axonal spheroids in the cerebellum granular layer and numerous necrotic neurons in the cerebral cortex and cerebellum molecular layer. This study demonstrates that Ipomoea asarifolia causes tremorgenic syndrome. The toxin is eliminated through milk and suckling lambs exhibit a greater susceptibility compared to adult animals.(AU)

Ointment of Ximenes americana promotes acceleration of wound healing in rats

Purpose:To evaluate the healing potential of the Ximenia americana hydroalcoholic extract in 10% cream in excisional wound models in rats.Methods:Sixty male adults Wistar rats were submitted to skin and subcutaneous tissue surgery in the right and left thoracic regions, divided into three experimental groups: Standard submitted to treatment with only the base vehicle, Treated wounds treated with hydroalcoholic extract of X. americana applied on 10%, Lanette base and Control, untreated wounds. The treatment was performed daily and the wounds evaluated microscopically by the quantification of fibroblasts, collagen fibers and blood vessels.Results:The histomorphometric analysis showed a significant increase in the number of fibroblasts, collagen fibers and blood vessels in the treated group.Conclusion:The topical action of the cream based on Ximenia americana shows angiogenic effects and improves the replacement of collagen, suggesting its use for the development of herbal remedy in the treatment of cutaneous wound healing.(AU)