Experimental infection of broiler chicks with Salmonella Typhimurium from pigeon (Columba livia) / Infecção experimental em pintos de corte com Salmonella Typhimurium proveniente de pombo (Columba livia)

Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results

Na literatura são relatados diversos casos de salmonelose em animais e humanos em vários países ocasionados pelo sorotipo Typhimurium. Em animais, a infeção subclínica favorece a disseminação do patógeno através das fezes. Nesse contexto, o pombo (Columba livia) com quadro assintomático pode desempenhar um importante papel na transmissão de salmonelose, mediante a excreção de fezes contaminadas em aviários comerciais ou em instalações de ração animal causando perdas econômicas para a indústria avícola, e proporcionando risco para a saúde pública. Objetivou-se avaliar a taxa de mortalidade, sinal clínico e a presença de Salmonella Typhimurium nas fezes e nos órgãos de pintinhos previamente inoculados com bactérias oriundas de pombo. Foram utilizados 70 pintos de corte de um dia de idade, os quais foram distribuídos aleatoriamente em dois grupos (G1 e G2) de 32 aves cada. Utilizou-se um grupo controle, constituído por seis aves, antes do início do tratamento para confirmação da ausência do patógeno. Utilizou-se inóculos de 0,4 e 0,7 mL contendo as concentrações de 105 e 106 unidades formadoras de colônias nas aves do grupo 1 (G1) e grupo 2 (G2), respectivamente. Nos dias 1, 4, 7 e 14 pós inoculação (dpi) foram coletados pool de fezes de cada gaiola, como também, swabs cloacais individuais. Ao 14 dpi, todos os pintos foram eutanasiados e coletados fígado, baço, pulmão, ceco e int

Survey of Salmonella sp. in budgerigars (Melopsittacus undulatus) in Fortaleza, Brazil / Survey of Salmonella sp. in budgerigars (Melopsittacus undulatus) in Fortaleza, Brazil

Background: The budgerigars (Melopsittacus undulatus) are psittacine of the Psittacidae family which belongs in the Psittaciformes order. These birds do not belong in the free living fauna of Brazil, therefore almost all of these birds found in the home environment are derived from pet stores or free markets. The dissemination of Salmonella among psittacine and other birds, and men as well, is a theme of great scientific concern. Salmonellosis is a highly contagious disease and psittacine are susceptible, but may also act as reservoirs hosting Salmonella sp. in the intestines and presenting as a source of infection to humans and domestic animals. Therefore, this study aimed to isolate, identify and to assess the dissemination of Salmonella in budgerigars in Fortaleza city. Materials, Methods & Results: This study was performed between January and September, 2011. Two hundred and sixty four samples of drag and cloacal swabs were collected from budgerigars found in pet stores and breeders, of which 198 were individual cloacal swabs, 58 were drag swabs and 8 intestine samples were also collected during necropsy performed in birds that died during the experiment. The microbiological procedure was performed according to the following steps: pre-enrichment step, using tubes containing peptone water broth; selective enrichment step, in which pre-enriched cultures were transferred

Background: The budgerigars (Melopsittacus undulatus) are psittacine of the Psittacidae family which belongs in the Psittaciformes order. These birds do not belong in the free living fauna of Brazil, therefore almost all of these birds found in the home environment are derived from pet stores or free markets. The dissemination of Salmonella among psittacine and other birds, and men as well, is a theme of great scientific concern. Salmonellosis is a highly contagious disease and psittacine are susceptible, but may also act as reservoirs hosting Salmonella sp. in the intestines and presenting as a source of infection to humans and domestic animals. Therefore, this study aimed to isolate, identify and to assess the dissemination of Salmonella in budgerigars in Fortaleza city. Materials, Methods & Results: This study was performed between January and September, 2011. Two hundred and sixty four samples of drag and cloacal swabs were collected from budgerigars found in pet stores and breeders, of which 198 were individual cloacal swabs, 58 were drag swabs and 8 intestine samples were also collected during necropsy performed in birds that died during the experiment. The microbiological procedure was performed according to the following steps: pre-enrichment step, using tubes containing peptone water broth; selective enrichment step, in which pre-enriched cultures were transferred

Survey of Salmonella sp. in budgerigars (Melopsittacus undulatus) in Fortaleza, Brazil / Survey of Salmonella sp. in budgerigars (Melopsittacus undulatus) in Fortaleza, Brazil

Background: The budgerigars (Melopsittacus undulatus) are psittacine of the Psittacidae family which belongs in the Psittaciformes order. These birds do not belong in the free living fauna of Brazil, therefore almost all of these birds found in the home environment are derived from pet stores or free markets. The dissemination of Salmonella among psittacine and other birds, and men as well, is a theme of great scientific concern. Salmonellosis is a highly contagious disease and psittacine are susceptible, but may also act as reservoirs hosting Salmonella sp. in the intestines and presenting as a source of infection to humans and domestic animals. Therefore, this study aimed to isolate, identify and to assess the dissemination of Salmonella in budgerigars in Fortaleza city. Materials, Methods & Results: This study was performed between January and September, 2011. Two hundred and sixty four samples of drag and cloacal swabs were collected from budgerigars found in pet stores and breeders, of which 198 were individual cloacal swabs, 58 were drag swabs and 8 intestine samples were also collected during necropsy performed in birds that died during the experiment. The microbiological procedure was performed according to the following steps: pre-enrichment step, using tubes containing peptone water broth; selective enrichment step, in which pre-enriched cultures were transferred

Background: The budgerigars (Melopsittacus undulatus) are psittacine of the Psittacidae family which belongs in the Psittaciformes order. These birds do not belong in the free living fauna of Brazil, therefore almost all of these birds found in the home environment are derived from pet stores or free markets. The dissemination of Salmonella among psittacine and other birds, and men as well, is a theme of great scientific concern. Salmonellosis is a highly contagious disease and psittacine are susceptible, but may also act as reservoirs hosting Salmonella sp. in the intestines and presenting as a source of infection to humans and domestic animals. Therefore, this study aimed to isolate, identify and to assess the dissemination of Salmonella in budgerigars in Fortaleza city. Materials, Methods & Results: This study was performed between January and September, 2011. Two hundred and sixty four samples of drag and cloacal swabs were collected from budgerigars found in pet stores and breeders, of which 198 were individual cloacal swabs, 58 were drag swabs and 8 intestine samples were also collected during necropsy performed in birds that died during the experiment. The microbiological procedure was performed according to the following steps: pre-enrichment step, using tubes containing peptone water broth; selective enrichment step, in which pre-enriched cultures were transferred

Experimental infection of broiler chicks with Salmonella Typhimurium from pigeon (Columba livia) / Infecção experimental em pintos de corte com Salmonella Typhimurium proveniente de pombo (Columba livia)

Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results

Na literatura são relatados diversos casos de salmonelose em animais e humanos em vários países ocasionados pelo sorotipo Typhimurium. Em animais, a infeção subclínica favorece a disseminação do patógeno através das fezes. Nesse contexto, o pombo (Columba livia) com quadro assintomático pode desempenhar um importante papel na transmissão de salmonelose, mediante a excreção de fezes contaminadas em aviários comerciais ou em instalações de ração animal causando perdas econômicas para a indústria avícola, e proporcionando risco para a saúde pública. Objetivou-se avaliar a taxa de mortalidade, sinal clínico e a presença de Salmonella Typhimurium nas fezes e nos órgãos de pintinhos previamente inoculados com bactérias oriundas de pombo. Foram utilizados 70 pintos de corte de um dia de idade, os quais foram distribuídos aleatoriamente em dois grupos (G1 e G2) de 32 aves cada. Utilizou-se um grupo controle, constituído por seis aves, antes do início do tratamento para confirmação da ausência do patógeno. Utilizou-se inóculos de 0,4 e 0,7 mL contendo as concentrações de 105 e 106 unidades formadoras de colônias nas aves do grupo 1 (G1) e grupo 2 (G2), respectivamente. Nos dias 1, 4, 7 e 14 pós inoculação (dpi) foram coletados pool de fezes de cada gaiola, como também, swabs cloacais individuais. Ao 14 dpi, todos os pintos foram eutanasiados e coletados fígado, baço, pulmão, ceco e int