Alterações histopatológicas testiculares associadas a infecção experimental por vírus Zika em macacos-de-cheiro / Testicular histopathological changes associated with experimental infection by zika virus in squirrel monkeys

The Zika virus belongs to the family Flaviviridae, genus Flavivirus. It is an arbovirus whose main vectors are mosquitoes of the genus Aedes. Studies in rodents have shown that the persistence of the virus in the testicles causes damage to the reproductive tissue. This work aimed to study the effect of experimental infection by the Zika virus on fertility in non-human primates of the species Saimiri collinsi. Five pre-pubertal males (≤2 years old) of the species Saimiri collinsi were used. Three animals were infected (infected group) with the strain of Zika virus BE H815744. Two other uninfected males were used as a negative control (uninfected group). Twenty-one days after infection, infected and uninfected males were euthanized. After euthanasia, they were referred for necroscopic examination for macroscopic and microscopic evaluation. During the necropsy, the testicles were collected and fixed in 10% formaldehyde. After fixation, the tissues were processed routinely and embedded in paraffin. The slides were stained with hematoxylin and eosin for histopathological evaluation. Histopathological changes were observed in the testis of three of the five animals. Different degrees of inflammation were identified, in addition to degeneration and/or necrosis. The three animals presented a reduced number of sperm cells, with no sperm and severe necrosis. The results obtained conclude that the Zika virus can cause pathological changes in the reproductive system of males of the species Saimiri collinsi.

Alterações histopatológicas testiculares associadas a infecção experimental por vírus Zika em macacos-de-cheiro / Testicular histopathological changes associated with experimental infection by zika virus in squirrel monkeys

The Zika virus belongs to the family Flaviviridae, genus Flavivirus. It is an arbovirus whose main vectors are mosquitoes of the genus Aedes. Studies in rodents have shown that the persistence of the virus in the testicles causes damage to the reproductive tissue. This work aimed to study the effect of experimental infection by the Zika virus on fertility in non-human primates of the species Saimiri collinsi. Five pre-pubertal males (≤2 years old) of the species Saimiri collinsi were used. Three animals were infected (infected group) with the strain of Zika virus BE H815744. Two other uninfected males were used as a negative control (uninfected group). Twenty-one days after infection, infected and uninfected males were euthanized. After euthanasia, they were referred for necroscopic examination for macroscopic and microscopic evaluation. During the necropsy, the testicles were collected and fixed in 10% formaldehyde. After fixation, the tissues were processed routinely and embedded in paraffin. The slides were stained with hematoxylin and eosin for histopathological evaluation. Histopathological changes were observed in the testis of three of the five animals. Different degrees of inflammation were identified, in addition to degeneration and/or necrosis. The three animals presented a reduced number of sperm cells, with no sperm and severe necrosis. The results obtained conclude that the Zika virus can cause pathological changes in the reproductive system of males of the species Saimiri collinsi.(AU)

Perfil da Inseminação artificial em tempo fixo, presença de muco e o status uterino correlacionado com a taxa de prenhez em vacas bubalinas no estado do Amapá / Profile of artificial insemination in fixed time, mucus and uterine status correlated with pregnancy rate in buffalo cows in Amapa state

The aim of this study was to correlate the profile of insemination, uterine status and the presence ofmucus on the day of insemination, with a pregnancy rate of buffalo cows in lowland area in the state of Amapá.This study was conducted at Fazenda general Moto LTDA., 148 km from the town of Macapa. The animals werekept in extensive grazing system. They used 48 animals received the following protocol: Day 0 (D0) to insert theintravaginal device P4 and 2.0 mg BE. At D9 was taken withdrawal of the device and application of 0.5 mg ofPGF2a and 400 IU eCG. In D11 was applied 0.004 mg GRnH and carried out insemination in D12, 64 hoursafter the withdrawal. The diagnosis of pregnancy was performed 30 days after artificial insemination. The datacollected from individual records were tabulated in spreadsheets. And evaluated in the SAS software (2000) 23buffaloes that had a positive pregnancy, 3 a bad AI profile, 18 a good AI profile and 2 excellent AI profile. 25cows got negative pregnancy, among them 4 demonstrated bad AI profile, 21 a good AI profile and no possessedexcellent AI profile. Regarding uterine Status- 46 animals (97.9%) had contracted uterus, however only 22(47.83%) animals were diagnosed with positive penhez. The variable presence of mucus, of 48 buffalos 21(43.75%) had such characteristics. The pregnancy rate was 22.9% (11) for the cows with mucus during theIATF. The variables profile of AI, mucus, uterine status, no positive influence on the pregnancy rate.(AU)

Taxa de prenhez em cabras da raça Saanen no nordeste do Pará submetidas à inseminação artificial em tempo fixo / Pregnancy rate in Saanen goats in northeast Pará submitted to artificial insemination in fixed time

Control of the estrous cycle through estrus synchronization protocols allows the producer to share lotsof females according to the management capacity and ownership of facilities easier handling and better use ofplayers. Therefore, the objective was to verify the influence of artificial insemination in fixed time (FTAI) withthe use of frozen semen in pregnancy rates of dairy goats. The study was conducted in the state of Pará, in themunicipality of Iguarapé-Acu, in a private property that is located in the northeastern region of Pará. 12 Saanengoats intended for milk production were used. Animals submitted to TAI with the use of six frozen semen becamepregnant, representing a pregnancy rate of 50%, which proved satisfactory results.(AU)

Perfil da Inseminação artificial em tempo fixo, presença de muco e o status uterino correlacionado com a taxa de prenhez em vacas bubalinas no estado do Amapá / Profile of artificial insemination in fixed time, mucus and uterine status correlated with pregnancy rate in buffalo cows in Amapa state

The aim of this study was to correlate the profile of insemination, uterine status and the presence ofmucus on the day of insemination, with a pregnancy rate of buffalo cows in lowland area in the state of Amapá.This study was conducted at Fazenda general Moto LTDA., 148 km from the town of Macapa. The animals werekept in extensive grazing system. They used 48 animals received the following protocol: Day 0 (D0) to insert theintravaginal device P4 and 2.0 mg BE. At D9 was taken withdrawal of the device and application of 0.5 mg ofPGF2a and 400 IU eCG. In D11 was applied 0.004 mg GRnH and carried out insemination in D12, 64 hoursafter the withdrawal. The diagnosis of pregnancy was performed 30 days after artificial insemination. The datacollected from individual records were tabulated in spreadsheets. And evaluated in the SAS software (2000) 23buffaloes that had a positive pregnancy, 3 a bad AI profile, 18 a good AI profile and 2 excellent AI profile. 25cows got negative pregnancy, among them 4 demonstrated bad AI profile, 21 a good AI profile and no possessedexcellent AI profile. Regarding uterine Status- 46 animals (97.9%) had contracted uterus, however only 22(47.83%) animals were diagnosed with positive penhez. The variable presence of mucus, of 48 buffalos 21(43.75%) had such characteristics. The pregnancy rate was 22.9% (11) for the cows with mucus during theIATF. The variables profile of AI, mucus, uterine status, no positive influence on the pregnancy rate.

Taxa de prenhez em cabras da raça Saanen no nordeste do Pará submetidas à inseminação artificial em tempo fixo / Pregnancy rate in Saanen goats in northeast Pará submitted to artificial insemination in fixed time

Control of the estrous cycle through estrus synchronization protocols allows the producer to share lotsof females according to the management capacity and ownership of facilities easier handling and better use ofplayers. Therefore, the objective was to verify the influence of artificial insemination in fixed time (FTAI) withthe use of frozen semen in pregnancy rates of dairy goats. The study was conducted in the state of Pará, in themunicipality of Iguarapé-Acu, in a private property that is located in the northeastern region of Pará. 12 Saanengoats intended for milk production were used. Animals submitted to TAI with the use of six frozen semen becamepregnant, representing a pregnancy rate of 50%, which proved satisfactory results.