Diluente quimicamente definido e antioxidantes polifenois na criopreservação de sêmen ovino / Chemically defined diluter and polyphenolic antioxidants in the cryopreservation of sheep semen

The objective of this study was to elaborate chemically defined extender based on casein, added with polyphenolic antioxidants, for the freezing of semen from ram breeders. To evaluate Tris-casein (5% glycerol), plus polyphenols, four semen pools from three rams sires were frozen (0µM; 10µM resveratrol; 5µM quercetin; 25µM catechin; 25µM catechin + 10µM resveratrol; 25µM catechin + 5µM quercetin; 10 µM resveratrol + 5µM quercetin; 25µM catechin + 10µM resveratrol + 5µM quercetin). After thawing, the samples were evaluated for sperm kinetics, plasma and acrosomal membrane integrity and mitochondrial membrane potential. There was no significant difference (p≥0.05) between the control group and the treatments for any of the evaluated parameters. However, it can be seen that the Tris-casein extender was efficient in cryopreserving ram semen. It is concluded that the Tris-casein extender can be used for freezing ram semen.

Efeito da adição de nanopartículas de Óxido de Zinco ao diluente de congelação do sêmen ovino / Effect of adding zinc oxide nanoparticles to the freezing extender of ram semen

The aim of this study was to evaluate the effect of adding zinc oxide nanoparticles to the ram semen cryopreservation diluter. Semen pools (n=6), from three Santa Inês breeders, were diluted in Tris-yolk (5% glycerol), supplemented with zinc oxide nanoparticles (0, 25, 75 and 150μg/mL) at a concentration of 200x10 6 sperm/mL. The samples were frozen in an automated system and stored in liquid nitrogen (-196 °C). At the timeof analysis, the semen samples were thawed (37 °C/30s) and evaluated for sperm kinetics, plasma and acrosomal membrane integrity and mitochondrial membrane potential. There was no significant difference (p>0.05) between the experimental groups in the parameters of kinetics, plasma membrane and acrosome integrity. As for the potential of mitochondrial membrane, all treated groups were significantly (p≤0.05) larger than the control. It was concluded that the zinc oxide nanoparticles increase the mitochondrial membrane potential of ram sperm submitted to the freezing/thawing process.

Diluente quimicamente definido e antioxidantes polifenois na criopreservação de sêmen ovino / Chemically defined diluter and polyphenolic antioxidants in the cryopreservation of sheep semen

The objective of this study was to elaborate chemically defined extender based on casein, added with polyphenolic antioxidants, for the freezing of semen from ram breeders. To evaluate Tris-casein (5% glycerol), plus polyphenols, four semen pools from three rams sires were frozen (0µM; 10µM resveratrol; 5µM quercetin; 25µM catechin; 25µM catechin + 10µM resveratrol; 25µM catechin + 5µM quercetin; 10 µM resveratrol + 5µM quercetin; 25µM catechin + 10µM resveratrol + 5µM quercetin). After thawing, the samples were evaluated for sperm kinetics, plasma and acrosomal membrane integrity and mitochondrial membrane potential. There was no significant difference (p≥0.05) between the control group and the treatments for any of the evaluated parameters. However, it can be seen that the Tris-casein extender was efficient in cryopreserving ram semen. It is concluded that the Tris-casein extender can be used for freezing ram semen.(AU)

Efeito da adição de nanopartículas de Óxido de Zinco ao diluente de congelação do sêmen ovino / Effect of adding zinc oxide nanoparticles to the freezing extender of ram semen

The aim of this study was to evaluate the effect of adding zinc oxide nanoparticles to the ram semen cryopreservation diluter. Semen pools (n=6), from three Santa Inês breeders, were diluted in Tris-yolk (5% glycerol), supplemented with zinc oxide nanoparticles (0, 25, 75 and 150μg/mL) at a concentration of 200x10 6 sperm/mL. The samples were frozen in an automated system and stored in liquid nitrogen (-196 °C). At the timeof analysis, the semen samples were thawed (37 °C/30s) and evaluated for sperm kinetics, plasma and acrosomal membrane integrity and mitochondrial membrane potential. There was no significant difference (p>0.05) between the experimental groups in the parameters of kinetics, plasma membrane and acrosome integrity. As for the potential of mitochondrial membrane, all treated groups were significantly (p≤0.05) larger than the control. It was concluded that the zinc oxide nanoparticles increase the mitochondrial membrane potential of ram sperm submitted to the freezing/thawing process.(AU)

Evaluation of the efficacy of electrolytes supplementation on dehydration related parameters in "vaquejada" horses / Avaliação da eficácia da suplementação eletrolítica sobre parâmetros relacionados a desidratação em equinos de vaquejada

The goal of this study was to evaluate the influence of the oral electrolytes supplementation in vaquejada horses on the clinical (cardiac and respiratory rate, rectal temperature, capillary refill time, and skin tent test) and laboratorial (hematocrit, total plasma proteins, and urinary density) parameters related to dehydration. Thirty horses were split into two groups, being one with no treatment (Control, n = 15) while the animals from the second group weretreated with electrolytes oral supplementation (Treated, n = 15). Clinical evaluation and biological sampling were performed in the horses during resting (T1), immediately after physical activity (T2), and four to five hours after physical activity (T3). Compared to Control, animals from Treated group presented better adaptation to the organic challenges induced by vaquejada,with special reference to higher urinary density for a prolonged period of time (P  0.05), the maintenance of total plasma protein throughout the evaluation time (P > 0.05), and reestablishment of the skin tent test in T3 similar to those observed in T1 (P > 0.05). It was concluded that oral electrolytic supplementation in vaquejada horses improves the organic response to dehydration.(AU)

O presente estudo objetivou avaliar a influência do uso de suplementação eletrolítica oral em cavalos de vaquejada sobre parâmetros clínicos (frequência cardíaca e respiratória, temperatura retal, tempo de enchimento capilar e teste de prega cutânea) e laboratoriais (hematócrito, proteínas plasmáticas totais e densidade urinária) que podem estar relacionados a desidratação. Trinta equinos foram divididos em dois grupos, sendo um sem tratamento (Controle, n = 15),enquanto os animais do segundo grupo receberam suplementação eletrolítica oral (Tratado, n = 15). As avaliações clínicas e coleta de material biológico para a análise forma realizadas com os animais em repouso (T1), imediatamente após a atividade física (T2) e quatro a cinco horas após a atividade física (T3). Comparado ao grupo Controle, os animais do grupo Tratado apresentaram resultados que demonstraram sua maior capacidade de adequação ao desafios orgânicos impostos pela prova de vaquejada, se refletindo principalmente no aumento da densidade urinária por período mais prolongado (P 0,05), ausência de elevação das proteínas plasmáticas totais ao longo dos períodos de avaliação (P > 0,05) e teste de prega cutânea em T3com valores similares aos observados em T1 (P > 0,05). Foi possível concluir que a suplementação eletrolítica.(AU)

Evaluation of the efficacy of electrolytes supplementation on dehydration related parameters in "vaquejada" horses / Avaliação da eficácia da suplementação eletrolítica sobre parâmetros relacionados a desidratação em equinos de vaquejada

The goal of this study was to evaluate the influence of the oral electrolytes supplementation in vaquejada horses on the clinical (cardiac and respiratory rate, rectal temperature, capillary refill time, and skin tent test) and laboratorial (hematocrit, total plasma proteins, and urinary density) parameters related to dehydration. Thirty horses were split into two groups, being one with no treatment (Control, n = 15) while the animals from the second group weretreated with electrolytes oral supplementation (Treated, n = 15). Clinical evaluation and biological sampling were performed in the horses during resting (T1), immediately after physical activity (T2), and four to five hours after physical activity (T3). Compared to Control, animals from Treated group presented better adaptation to the organic challenges induced by vaquejada,with special reference to higher urinary density for a prolonged period of time (P  0.05), the maintenance of total plasma protein throughout the evaluation time (P > 0.05), and reestablishment of the skin tent test in T3 similar to those observed in T1 (P > 0.05). It was concluded that oral electrolytic supplementation in vaquejada horses improves the organic response to dehydration.

O presente estudo objetivou avaliar a influência do uso de suplementação eletrolítica oral em cavalos de vaquejada sobre parâmetros clínicos (frequência cardíaca e respiratória, temperatura retal, tempo de enchimento capilar e teste de prega cutânea) e laboratoriais (hematócrito, proteínas plasmáticas totais e densidade urinária) que podem estar relacionados a desidratação. Trinta equinos foram divididos em dois grupos, sendo um sem tratamento (Controle, n = 15),enquanto os animais do segundo grupo receberam suplementação eletrolítica oral (Tratado, n = 15). As avaliações clínicas e coleta de material biológico para a análise forma realizadas com os animais em repouso (T1), imediatamente após a atividade física (T2) e quatro a cinco horas após a atividade física (T3). Comparado ao grupo Controle, os animais do grupo Tratado apresentaram resultados que demonstraram sua maior capacidade de adequação ao desafios orgânicos impostos pela prova de vaquejada, se refletindo principalmente no aumento da densidade urinária por período mais prolongado (P 0,05), ausência de elevação das proteínas plasmáticas totais ao longo dos períodos de avaliação (P > 0,05) e teste de prega cutânea em T3com valores similares aos observados em T1 (P > 0,05). Foi possível concluir que a suplementação eletrolítica.