Renal biomarkers of male and female Wistar rats (Rattus norvegicus) undergoing renal ischemia and reperfusion

PURPOSE: To investigate biomarkers of acute renal injury in Wistar rats, subjected to left renal ischemia for 10 minutes, and then compare reperfusion at 24 hours, and at 5, 7, 14 and 21 days after the procedure.METHODS: Eight female and male rats between 60 and 81 days old were used in the Central Animal Facility of the UFMS. Assessed biomarkers included urine protein, urea, creatinine, glucose, sodium, potassium, urine alkaline phosphatase and gamma-glutamyl transferase activities, and protein-to-creatinine ratio; and in serum: urea, creatinine, sodium and potassium, fractional excretion of sodium, potassium, urine flow and creatinine clearance.RESULTS:Greater variance was observed in the parameters at 24 hours and at five days (p 0.05) after reperfusion. On the 21st day, these parameters approximated those obtained for the control group.CONCLUSIONS:Renal ischemia for 10 minutes was sufficient to raise urine levels of protein, glucose, fractional excretion of potassium, urea, creatinine clearance, urine activity of gamma-glutamyltransferase and alkaline phosphatase enzymes in the first 24 hours, up to five days after reperfusion, which may indicate risk of acute kidney injury, according to the RIFLE classification.(AU)

Biochemical and hematological parameters in mice (Mus musculus) from the UFMS central laboratory animal facilities / Determinação de parâmetros bioquímicos e hematológicos em camundongos (Mus musculus) do biotério central da UFMS

This study evaluated the biochemical and hematological profiles of Swiss Webster mice reared at the Central Laboratory Animal Facilities of the Universidade Federal de Mato Grosso do Sul. Blood samples were drawn from 18 animals (10 males, 8 females) aged 180 days and 16 animals (8 males, 8 females) aged 60 days, all of them of monitored conventional sanitary status. The hematological variables investigated were RBC count, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelet count, and total and differential WBC counts. The biochemical variables were total protein, albumin, alkaline phosphatase, urea, creatinine, alanine aminotransferase, and aspartate aminotransferase. Most results lay within the normal range for the species. Biochemical and hematological reference values for each colony are important because the results of exams can influenced by sex, strain, genotype, age, diet, handling, and environment, among other factors.

Este estudo teve como objetivo avaliar os perfis bioquímicos e hematológicos de camundongos Swiss Webster do Biotério Central da Universidade Federal de Mato Grosso do Sul. Foram utilizados 18 animais de 180 dias de idade (10 machos e 8 fêmeas) e 16 de 60 dias (8 machos e 8 fêmeas), todos de padrão sanitário convencional monitorizado, dos quais se coletou sangue para a realização de provas bioquímicas e hematológicas. Os parâmetros hematológicos analisados foram: contagem de hemácias, hemoglobina, volume corpuscular médio, hemoglobina corpuscular média, concentração de hemoglobina corpuscular média, contagem de plaquetas, contagem total de leucócitos e diferencial de células. Os parâmetros bioquímicos foram: proteína total, albumina, fosfatase alcalina, ureia, creatinina, alanina aminotransferase e aspartato aminotransferase. A maioria dos valores obtidos situou-se dentro da faixa de variação normal para a espécie. A importância de determinar valores de referência para exames laboratoriais de cada colônia animal deve ser considerada, pois os resultados podem ser influenciados por sexo, linhagem, genótipo, idade, dieta, manuseio e ambiente, entre outros fatores.

Biochemical and hematological parameters in mice (Mus musculus) from the UFMS central laboratory animal facilities / Determinação de parâmetros bioquímicos e hematológicos em camundongos (Mus musculus) do biotério central da UFMS

This study evaluated the biochemical and hematological profiles of Swiss Webster mice reared at the Central Laboratory Animal Facilities of the Universidade Federal de Mato Grosso do Sul. Blood samples were drawn from 18 animals (10 males, 8 females) aged 180 days and 16 animals (8 males, 8 females) aged 60 days, all of them of monitored conventional sanitary status. The hematological variables investigated were RBC count, hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, platelet count, and total and differential WBC counts. The biochemical variables were total protein, albumin, alkaline phosphatase, urea, creatinine, alanine aminotransferase, and aspartate aminotransferase. Most results lay within the normal range for the species. Biochemical and hematological reference values for each colony are important because the results of exams can influenced by sex, strain, genotype, age, diet, handling, and environment, among other factors.(AU)

Este estudo teve como objetivo avaliar os perfis bioquímicos e hematológicos de camundongos Swiss Webster do Biotério Central da Universidade Federal de Mato Grosso do Sul. Foram utilizados 18 animais de 180 dias de idade (10 machos e 8 fêmeas) e 16 de 60 dias (8 machos e 8 fêmeas), todos de padrão sanitário convencional monitorizado, dos quais se coletou sangue para a realização de provas bioquímicas e hematológicas. Os parâmetros hematológicos analisados foram: contagem de hemácias, hemoglobina, volume corpuscular médio, hemoglobina corpuscular média, concentração de hemoglobina corpuscular média, contagem de plaquetas, contagem total de leucócitos e diferencial de células. Os parâmetros bioquímicos foram: proteína total, albumina, fosfatase alcalina, ureia, creatinina, alanina aminotransferase e aspartato aminotransferase. A maioria dos valores obtidos situou-se dentro da faixa de variação normal para a espécie. A importância de determinar valores de referência para exames laboratoriais de cada colônia animal deve ser considerada, pois os resultados podem ser influenciados por sexo, linhagem, genótipo, idade, dieta, manuseio e ambiente, entre outros fatores.(AU)