Aspects and mechanisms of low fertility in anovulatory dairy cows

Postpartum anovulation is a natural process that is observed in most mammals, including women. In lactating dairy cows, the interval from calving to first ovulation typically averages 4 to 5 weeks, but a substantial proportion of cows have not resumed estrous cyclicity by 60 days postpartum. Extended delay in resumption of first postpartum ovulation is known to exert long-lasting detrimental effects on fertility in dairy cows including the lack of spontaneous estrus and subsequent timely insemination postpartum, but when anovular cows have the estrous cycle synchronized for artificial insemination (AI), still pregnancy per AI is reduced and the risk of pregnancy loss increased. Many risk factors exist for extended postpartum anovulatory periods such as negative nutrient balance and diseases, and these risk factors are also known to depress fertility by themselves. A key feature in anovular cows when inseminated is that they develop the ovulatory follicle under subluteal or low concentrations of progesterone. Progesterone from the corpus luteum is pivotal for follicle development, oocyte competence, embryo growth, and endometrial function; however, many of these effects exerted by progesterone are mediated either by secretion of gonadotropins influencing follicular function and oocyte competence or by endometrial histotroph secretion influencing embryo/conceptus growth and developmental biology

Aspects and mechanisms of low fertility in anovulatory dairy cows

Postpartum anovulation is a natural process that is observed in most mammals, including women. In lactating dairy cows, the interval from calving to first ovulation typically averages 4 to 5 weeks, but a substantial proportion of cows have not resumed estrous cyclicity by 60 days postpartum. Extended delay in resumption of first postpartum ovulation is known to exert long-lasting detrimental effects on fertility in dairy cows including the lack of spontaneous estrus and subsequent timely insemination postpartum, but when anovular cows have the estrous cycle synchronized for artificial insemination (AI), still pregnancy per AI is reduced and the risk of pregnancy loss increased. Many risk factors exist for extended postpartum anovulatory periods such as negative nutrient balance and diseases, and these risk factors are also known to depress fertility by themselves. A key feature in anovular cows when inseminated is that they develop the ovulatory follicle under subluteal or low concentrations of progesterone. Progesterone from the corpus luteum is pivotal for follicle development, oocyte competence, embryo growth, and endometrial function; however, many of these effects exerted by progesterone are mediated either by secretion of gonadotropins influencing follicular function and oocyte competence or by endometrial histotroph secretion influencing embryo/conceptus growth and developmental biology…(AU)

Impact of animal health on reproduction of dairy cows

[...] A wealth of information in the scientific literature is available linking diseases with depressed reproduction in dairy cows. Unfortunately, only few studies have established a causal relationship between a specific disease and fertility, and little is known about the mechanisms that underlie the decrease in pregnancy in dairy cows that had disease in early lactation. It is clear that dairy cows that suffer from disease processes have impaired resumption of postpartum ovulation, compromised fertilization and pre-and peri-implantation conceptus development, altered conceptus gene expression, increased pregnancy loss and, ultimately reduced pregnancy per insemination that causes an extension in time to pregnancy. Because mechanisms are poorly understood, no target intervention is available at this time to reverse the poor reproduction in cows that develop periparturient diseases, except methods to induce cyclicity in anovular cows or to improve insemination rate in cows not detected in estrus. Regardless of a better understanding of the underlying biology of poor fertility in diseased cows, a pivotal approach is to implement strategies that mitigate the risk factors that predispose cows to disease. Such interventions include, but are not limited to, improving transition cow management and grouping, proper dietary formulation to prevent periparturient diseases associated with intermediary and mineral metabolism, strategies for reducing calving-related disorders, and methods to prevent mastitis and lameness. Future developments in target strategies to improve reproduction of cows suffering from peripartum diseases will require a better understanding of the impaired biological processes that compromise establishment and maintenance of pregnancy in this subfertile population of cows.

Impact of animal health on reproduction of dairy cows

[...] A wealth of information in the scientific literature is available linking diseases with depressed reproduction in dairy cows. Unfortunately, only few studies have established a causal relationship between a specific disease and fertility, and little is known about the mechanisms that underlie the decrease in pregnancy in dairy cows that had disease in early lactation. It is clear that dairy cows that suffer from disease processes have impaired resumption of postpartum ovulation, compromised fertilization and pre-and peri-implantation conceptus development, altered conceptus gene expression, increased pregnancy loss and, ultimately reduced pregnancy per insemination that causes an extension in time to pregnancy. Because mechanisms are poorly understood, no target intervention is available at this time to reverse the poor reproduction in cows that develop periparturient diseases, except methods to induce cyclicity in anovular cows or to improve insemination rate in cows not detected in estrus. Regardless of a better understanding of the underlying biology of poor fertility in diseased cows, a pivotal approach is to implement strategies that mitigate the risk factors that predispose cows to disease. Such interventions include, but are not limited to, improving transition cow management and grouping, proper dietary formulation to prevent periparturient diseases associated with intermediary and mineral metabolism, strategies for reducing calving-related disorders, and methods to prevent mastitis and lameness. Future developments in target strategies to improve reproduction of cows suffering from peripartum diseases will require a better understanding of the impaired biological processes that compromise establishment and maintenance of pregnancy in this subfertile population of cows. (AU)

Equine seminal plasma on preserving the viability of frozen-thawed ram sperm

Re-suspension of frozen-thawed ram sperm with ovine and bovine whole seminal plasma (SP) is beneficial for its post-thawing viability. However, neither the influence of SP incubation duration nor the re-suspension with equine SP has been tested. In the first experiment, frozen-thawed ram sperm were incubated with SP from bovine (BSP), equine (ESP) or ovine (OSP) or without SP (-SP) for short (5 min) or long (6 h) periods. Viability parameters such as sperm progressive motility, percentage of live cells and membrane functionality were assessed every 2 h for 6 h. All SP treatments showed higher spermatozoa viability than the -SP treatment in most evaluations. Incubation time did not affect sperm viability for BSP treatment, however, ESP and OSP induced a transitory benefic effect in the short incubation period and detrimental effect in the longer period. In the second experiment, frozen-thawed sperm, with or without short incubation with SP, were selected by swim-up, and their DNA fragmentation rate was assessed using comet assay immediately after swim-up completion and after 5 h of incubation. BSP, ESP and OSP protein profiles were determined by SDS-PAGE. Only ESP was associated with sperm DNA stabilization capacity and SP from rams and bulls showed protein profiles different from that of stallions. These experiments indicate that equine or ovine, but not bovine whole SP supplementation to post-thawing incubation medium of frozen-thawed ram sperm affects its viability in a time dependent manner. The beneficial effect of ESP on stabilizing DNA integrity, even after sperm washing with swim-up method and incubation for 5 h, can be determined by SPP or by antioxidant components from SP.

Equine seminal plasma on preserving the viability of frozen-thawed ram sperm

Re-suspension of frozen-thawed ram sperm with ovine and bovine whole seminal plasma (SP) is beneficial for its post-thawing viability. However, neither the influence of SP incubation duration nor the re-suspension with equine SP has been tested. In the first experiment, frozen-thawed ram sperm were incubated with SP from bovine (BSP), equine (ESP) or ovine (OSP) or without SP (-SP) for short (5 min) or long (6 h) periods. Viability parameters such as sperm progressive motility, percentage of live cells and membrane functionality were assessed every 2 h for 6 h. All SP treatments showed higher spermatozoa viability than the -SP treatment in most evaluations. Incubation time did not affect sperm viability for BSP treatment, however, ESP and OSP induced a transitory benefic effect in the short incubation period and detrimental effect in the longer period. In the second experiment, frozen-thawed sperm, with or without short incubation with SP, were selected by swim-up, and their DNA fragmentation rate was assessed using comet assay immediately after swim-up completion and after 5 h of incubation. BSP, ESP and OSP protein profiles were determined by SDS-PAGE. Only ESP was associated with sperm DNA stabilization capacity and SP from rams and bulls showed protein profiles different from that of stallions. These experiments indicate that equine or ovine, but not bovine whole SP supplementation to post-thawing incubation medium of frozen-thawed ram sperm affects its viability in a time dependent manner. The beneficial effect of ESP on stabilizing DNA integrity, even after sperm washing with swim-up method and incubation for 5 h, can be determined by SPP or by antioxidant components from SP.(AU)

Economic aspects of applying reproductive technologies to dairy herds

Reproduction continues to be a critical component to maintain a dairy farm economically viable. For every farm and for every cow, there is an optimum time for pregnancy, which is mostly influenced by level of production, persistency of lactation, and parity. In general, as production decreases, lactation number increases, and persistency of lactation decreases, cows should be bred sooner postpartum and pregnancy obtained early in lactation. The voluntary waiting period is determined based on the desired interval postpartum to pregnancy and the pregnancy rate of the farm. As pregnancy rates increase, the voluntary waiting period can be delayed, particularly when milk production is high. Studies in the literature have compared several breeding strategies to obtain a pregnant cow. In general, pregnancies obtained by artificial insemination are cheaper than those originated by natural service. The major reason is that AI programs result in similar or better reproductive performance and are cheaper to implement than natural service programs because of the high costs of acquiring and feeding bulls. Within the AI program, those that incorporate timed AI for first insemination followed by detection of estrus result in lowest median days open and more profit per cow, and the benefits of improving reproduction are greater when milk prices ar e low. The use of embryo technologies as a breeding program for lactating dairy cows, with the aim to improve reproductive performance, is only attractive when the differential in fertility relative to AI is large. In most cases, AI programs have to result in very poor fertility (<15%) for the typical results from embryo transfer (40-45% pregnancy) to be economically attractive at current costs. For dairy heifers, there is littl e justification to incorporate timed AI programs when detection of estrus is excellent, above 70%; however, for farms with detection of estrus below 60%, either timed AI for first AI followed by detection of estrus or timed AI alone improve reproductive performance and reduce the cost per pregnancy.

Economic aspects of applying reproductive technologies to dairy herds

Reproduction continues to be a critical component to maintain a dairy farm economically viable. For every farm and for every cow, there is an optimum time for pregnancy, which is mostly influenced by level of production, persistency of lactation, and parity. In general, as production decreases, lactation number increases, and persistency of lactation decreases, cows should be bred sooner postpartum and pregnancy obtained early in lactation. The voluntary waiting period is determined based on the desired interval postpartum to pregnancy and the pregnancy rate of the farm. As pregnancy rates increase, the voluntary waiting period can be delayed, particularly when milk production is high. Studies in the literature have compared several breeding strategies to obtain a pregnant cow. In general, pregnancies obtained by artificial insemination are cheaper than those originated by natural service. The major reason is that AI programs result in similar or better reproductive performance and are cheaper to implement than natural service programs because of the high costs of acquiring and feeding bulls. Within the AI program, those that incorporate timed AI for first insemination followed by detection of estrus result in lowest median days open and more profit per cow, and the benefits of improving reproduction are greater when milk prices ar e low. The use of embryo technologies as a breeding program for lactating dairy cows, with the aim to improve reproductive performance, is only attractive when the differential in fertility relative to AI is large. In most cases, AI programs have to result in very poor fertility (<15%) for the typical results from embryo transfer (40-45% pregnancy) to be economically attractive at current costs. For dairy heifers, there is littl e justification to incorporate timed AI programs when detection of estrus is excellent, above 70%; however, for farms with detection of estrus below 60%, either timed AI for first AI followed by detection of estrus or timed AI alone improve reproductive performance and reduce the cost per pregnancy.(AU)

Influences of nutrition and metabo lism on fertility of dairy cows

During early postpartum, high-producing dairy cows undergo a period of extensive tissue catabolism because of negative nutrien t balance. Homeorrhetic controls assure that nutrients are partitioned to favor lactation at the same time that homeostasis secures survival. However, unrestrained metabolic disturbances often lead to diseases which, in turn, dramatically decrease both productive and reproductive performance. Negative nutrient balance ha s been associated with compromised immune and reproductive functions in dairy cows. Low circulating concentrations of glucose and insulin associated with elevated concentrations of non-esterified fatty acids and ketone bodies postpartum have disruptive and detrimental effects on the oocyte, granulosa and immune cells. Negative nutrient balance is associated with changes in the pattern of ovarian follicle growth which can indirectly affect oocyte quality. Some of this disruption seems to be the result of endocrine and biochemical changes that alter the micro- environment of the growing and maturing oocyte. In addition, cows under negative nutrient balance have extended periods of anovulation. Postpartum anestrus, as well as infertility, is magnified by losses of body condition during the early postpartum period. The underlying mechanism for resumption of ovulatory cycles seems to be associated with metabolic signals and regulatory hormones primarily insulin and insulin- like growth factor (IGF)-1, which link nutritional status with gonadotropin secretion, recoupling of the growth hormone-IGF system, and follicle maturation and ovulation. Feeding diets th at promote increases in plasma glucose and insulin may improve the metabolic and endocrine status of cows in early lactation. Furthermore, fertility in postpartum cows is also determined by uterine health. Reductions in circulating concentrations of Ca and antioxidant vitamins around parturition are also linked with impaired immune competence and result in greater risk of uterine diseases that impair reproduction. Specific nutrients and dietary ingredients have been implicated to affect reproduction in cattle. Excess intake of dietary protein has been suggested as detrimental to fertility, although feeding excess of dietary protein can no longer be justified. Addition of moderate amounts of supplemental fat to the diet improves caloric intake, modulates prostaglandin F2  secretion by the uterus, affects ovarian dynamics, enhances luteal function and embryo quality, and has moderate positive effects on fertility. More specifically, some fatty acids might impact fertilization rate and embryo quality in dairy cows. On the contrary, some dietary ingredients, such as gossypol, when ingested in large quantities decrease fertility of dairy cows because of its negative effects on embryo quality and pregnancy maintenance.

Influences of nutrition and metabo lism on fertility of dairy cows

During early postpartum, high-producing dairy cows undergo a period of extensive tissue catabolism because of negative nutrien t balance. Homeorrhetic controls assure that nutrients are partitioned to favor lactation at the same time that homeostasis secures survival. However, unrestrained metabolic disturbances often lead to diseases which, in turn, dramatically decrease both productive and reproductive performance. Negative nutrient balance ha s been associated with compromised immune and reproductive functions in dairy cows. Low circulating concentrations of glucose and insulin associated with elevated concentrations of non-esterified fatty acids and ketone bodies postpartum have disruptive and detrimental effects on the oocyte, granulosa and immune cells. Negative nutrient balance is associated with changes in the pattern of ovarian follicle growth which can indirectly affect oocyte quality. Some of this disruption seems to be the result of endocrine and biochemical changes that alter the micro- environment of the growing and maturing oocyte. In addition, cows under negative nutrient balance have extended periods of anovulation. Postpartum anestrus, as well as infertility, is magnified by losses of body condition during the early postpartum period. The underlying mechanism for resumption of ovulatory cycles seems to be associated with metabolic signals and regulatory hormones primarily insulin and insulin- like growth factor (IGF)-1, which link nutritional status with gonadotropin secretion, recoupling of the growth hormone-IGF system, and follicle maturation and ovulation. Feeding diets th at promote increases in plasma glucose and insulin may improve the metabolic and endocrine status of cows in early lactation. Furthermore, fertility in postpartum cows is also determined by uterine health. Reductions in circulating concentrations of Ca and antioxidant vitamins around parturition are also linked with impaired immune competence and result in greater risk of uterine diseases that impair reproduction. Specific nutrients and dietary ingredients have been implicated to affect reproduction in cattle. Excess intake of dietary protein has been suggested as detrimental to fertility, although feeding excess of dietary protein can no longer be justified. Addition of moderate amounts of supplemental fat to the diet improves caloric intake, modulates prostaglandin F2  secretion by the uterus, affects ovarian dynamics, enhances luteal function and embryo quality, and has moderate positive effects on fertility. More specifically, some fatty acids might impact fertilization rate and embryo quality in dairy cows. On the contrary, some dietary ingredients, such as gossypol, when ingested in large quantities decrease fertility of dairy cows because of its negative effects on embryo quality and pregnancy maintenance.(AU)