Resumo
The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae) was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL) showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of eggs eclosion average and 65.4% of effectiveness. HPLC analysis revealed the presence of phenolic compounds, which may be related to biological activity shown by the extracts, which can be used as an alternative control against R. microplus adult tick.(AU)
A atividade acaricida do extrato etanólico bruto e das frações das folhas de Morus nigra (Moraceae) foi avaliada no carrapato Rhipicephalus microplus, utilizando-se o teste de imersão de fêmeas ingurgitadas. Foram avaliadas a mortalidade e a fertilidade das fêmeas ingurgitadas, expostas a diferentes concentrações (5, 10 e 25 mg/mL) das frações hexânica, clorofórmica e acetato de etila, bem como do extrato etanólico. Os testes foram realizados em triplicata. O estudo também identificou os principais compostos fenólicos do extrato e das frações dessa espécie por HPLC. A fração clorofórmica das folhas de M. nigra (25mg/mL) apresentou o melhor resultado para essa espécie, obtendo-se 62,6% de inibição da oviposição, 39,3% da eclosão dos ovos e 65,4% de eficácia. A análise por HPLC revelou a presença de compostos fenólicos, os quais podem estar relacionados com a atividade biológica demonstrada pelos extratos, que podem ser utilizados como auxiliar no controle contra carrapato adulto de R. microplus.(AU)
Assuntos
Animais , Acaricidas/análise , Morus/toxicidade , Extratos Vegetais/uso terapêutico , Rhipicephalus/efeitos dos fármacos , Controle de Ácaros e Carrapatos/métodos , Cromatografia Líquida de Alta Pressão/veterinária , Carrapatos/efeitos dos fármacosResumo
The acaricidal activity of crude ethanolic extract and fractions from the leaves of Morus nigra (Moraceae) was carried out on female cattle ticks Rhipicephalus microplus, using the adult immersion test. The mortality and fertility of females exposed to different concentrations of hexane, chloroform and ethyl acetate fractions, as well as ethanolic extract of M. nigra with concentrations of 5, 10 and 25mg/ml were evaluated using three treatment groups, two control groups and triplicate tests. The study also identified the main phenolic compounds of the extract and fractions of this species by HPLC. The chloroform fraction of leaves of M. nigra (25mg/mL) showed the best results for this species, obtaining 62.6% of inhibition of oviposition, 39.3% of eggs eclosion average and 65.4% of effectiveness. HPLC analysis revealed the presence of phenolic compounds, which may be related to biological activity shown by the extracts, which can be used as an alternative control against R. microplus adult tick.(AU)
A atividade acaricida do extrato etanólico bruto e das frações das folhas de Morus nigra (Moraceae) foi avaliada no carrapato Rhipicephalus microplus, utilizando-se o teste de imersão de fêmeas ingurgitadas. Foram avaliadas a mortalidade e a fertilidade das fêmeas ingurgitadas, expostas a diferentes concentrações (5, 10 e 25 mg/mL) das frações hexânica, clorofórmica e acetato de etila, bem como do extrato etanólico. Os testes foram realizados em triplicata. O estudo também identificou os principais compostos fenólicos do extrato e das frações dessa espécie por HPLC. A fração clorofórmica das folhas de M. nigra (25mg/mL) apresentou o melhor resultado para essa espécie, obtendo-se 62,6% de inibição da oviposição, 39,3% da eclosão dos ovos e 65,4% de eficácia. A análise por HPLC revelou a presença de compostos fenólicos, os quais podem estar relacionados com a atividade biológica demonstrada pelos extratos, que podem ser utilizados como auxiliar no controle contra carrapato adulto de R. microplus.(AU)
Assuntos
Animais , Acaricidas/análise , Morus/toxicidade , Rhipicephalus , Controle de Ácaros e Carrapatos/métodos , Extratos Vegetais/uso terapêutico , Carrapatos , Cromatografia Líquida de Alta Pressão/veterináriaResumo
The aim of the present study was to evaluate the effect of Amburana cearensis extract during caprine ovarian tissue transportation on the survival of preantral follicles in vitro. HPLC was used to determine the fingerprint chromatogram of the ethanolic extract. Five goat ovarian pairs were divided into fragments. One fragment was fixed for histology and TUNEL Analysis (fresh control). The other fragments were placed in MEM or A. cearensis extract (0.1; 0.2 or 0.4 mg/ml) and stored at 4oC for 6, 12 or 24 h. Preserved Fragments were also fixed for histology and TUNEL analysis. The presence of phenolic compounds (protocatechuic acid, epicatechin, p-coumaric acid, gallic acid and kaempferol) in the extract was confirmed using HPLC. Thepercentage of normal follicles preserved in 0.2 mg/ml A. cearensis for 6 h was similar to that observed in the fresh control. Moreover, the percentage of normal follicles was higher after preservation in 0.2 mg/ml A. cearensisFor 6 h than the other A. cearensis treatments and similar to that found in MEM. There were no differences in the percentage of apoptotic cells between fresh control and those preserved for 6 h in MEM or 0.2 mg/ml A. cearensis. In conclusion, both 0.2 mg/ml A. cearensis or MEM can be used for the preservation of goat preantral follicles for up to 6 h. The use of A. cearenses is recommended due to the higher cost of MEM.
Assuntos
Feminino , Animais , Cabras/embriologia , Cabras/fisiologia , Folículo Ovariano/embriologia , HistologiaResumo
The aim of the present study was to evaluate the effect of Amburana cearensis extract during caprine ovarian tissue transportation on the survival of preantral follicles in vitro. HPLC was used to determine the fingerprint chromatogram of the ethanolic extract. Five goat ovarian pairs were divided into fragments. One fragment was fixed for histology and TUNEL Analysis (fresh control). The other fragments were placed in MEM or A. cearensis extract (0.1; 0.2 or 0.4 mg/ml) and stored at 4oC for 6, 12 or 24 h. Preserved Fragments were also fixed for histology and TUNEL analysis. The presence of phenolic compounds (protocatechuic acid, epicatechin, p-coumaric acid, gallic acid and kaempferol) in the extract was confirmed using HPLC. Thepercentage of normal follicles preserved in 0.2 mg/ml A. cearensis for 6 h was similar to that observed in the fresh control. Moreover, the percentage of normal follicles was higher after preservation in 0.2 mg/ml A. cearensisFor 6 h than the other A. cearensis treatments and similar to that found in MEM. There were no differences in the percentage of apoptotic cells between fresh control and those preserved for 6 h in MEM or 0.2 mg/ml A. cearensis. In conclusion, both 0.2 mg/ml A. cearensis or MEM can be used for the preservation of goat preantral follicles for up to 6 h. The use of A. cearenses is recommended due to the higher cost of MEM.(AU)