Toxicidade reprodutiva da associação de itraconazol e beta-glucana em ratas e sua progênie / Reproductive toxicity of itraconazole in combination with beta-glucan in female rats and their offspring

Background: Reports of yeast isolates resistant to traditional antifungal drugs have become common. Similarly, refractory clinical cases treated with these traditional antifungal drugs have also been reported. These cases ‘may or may not be related to pregnancy. Newly developed therapeutic approaches, such as the immunostimulant β-glucan combined with the traditional antifungal agents show promising results. Therefore, knowledge of the effects of these new associations is essential. The aims of this study were to evaluate the effects of the combination of itraconazole and β (1-3) glucan on fertility in female rats and its interference in the development of their offspring, including teratogenic potential.Materials, Methods & Results: A total of 180 female Wistar rats (90 days old) separated into six groups were used (n = 30 per group): Negative Control - treated daily with the volume corresponding to 10 mL.kg-1 of sterile distilled water orally, and 0.25 mL of sterile 0.9% NaCl solution subcutaneously weekly; IT - treated daily with itraconazole at a dose of 10 mg.kg-1 orally, and 0.25 ml of sterile 0.9% NaCl solution subcutaneously weekly; Beta - treated daily with the volume corresponding to 10 mL.kg-1 of sterile distilled water orally, and 0.5 mg of β (1-3) glucan subcutaneously weekly; DT - treated daily with itraconazole at a dose of 10 mg.kg-1 orally, and 0.5 mg of β (1-3) glucan subcutaneously weekly; DT5x - treated daily with itraconazole at a dose of 50 mg.kg-1 orally, and 0.5 mg of β (1-3) glucan subcutaneously weekly; DT10x - treated daily with itraconazole at a dose of 100 mg.kg-1 orally, and 0.5 mg of β (1-3) glucan subcutaneously weekly. The rats were treated before (14 days) and during the mating period (up to 21 days), pregnancy (21 days) and lactation (21 days).[...](AU)

Toxicidade reprodutiva da associação de itraconazol e beta-glucana em ratas e sua progênie / Reproductive toxicity of itraconazole in combination with beta-glucan in female rats and their offspring

Background: Reports of yeast isolates resistant to traditional antifungal drugs have become common. Similarly, refractory clinical cases treated with these traditional antifungal drugs have also been reported. These cases ‘may or may not be related to pregnancy. Newly developed therapeutic approaches, such as the immunostimulant β-glucan combined with the traditional antifungal agents show promising results. Therefore, knowledge of the effects of these new associations is essential. The aims of this study were to evaluate the effects of the combination of itraconazole and β (1-3) glucan on fertility in female rats and its interference in the development of their offspring, including teratogenic potential.Materials, Methods & Results: A total of 180 female Wistar rats (90 days old) separated into six groups were used (n = 30 per group): Negative Control - treated daily with the volume corresponding to 10 mL.kg-1 of sterile distilled water orally, and 0.25 mL of sterile 0.9% NaCl solution subcutaneously weekly; IT - treated daily with itraconazole at a dose of 10 mg.kg-1 orally, and 0.25 ml of sterile 0.9% NaCl solution subcutaneously weekly; Beta - treated daily with the volume corresponding to 10 mL.kg-1 of sterile distilled water orally, and 0.5 mg of β (1-3) glucan subcutaneously weekly; DT - treated daily with itraconazole at a dose of 10 mg.kg-1 orally, and 0.5 mg of β (1-3) glucan subcutaneously weekly; DT5x - treated daily with itraconazole at a dose of 50 mg.kg-1 orally, and 0.5 mg of β (1-3) glucan subcutaneously weekly; DT10x - treated daily with itraconazole at a dose of 100 mg.kg-1 orally, and 0.5 mg of β (1-3) glucan subcutaneously weekly. The rats were treated before (14 days) and during the mating period (up to 21 days), pregnancy (21 days) and lactation (21 days).[...]

Efeitos da associação de beta-glucana e itraconazol sobre a fertilidade de ratos Wistar machos / Effects of the Association of Beta-glucan and Itraconazole on Fertility of Male Wistar Rats

Background: In recent years, the number of patients with systemic fungal infections requiring antifungal therapy has increased. As a consequence, antimicrobial resistance to conventional treatment is frequently reported. Due to this reason, new therapies emerge, including the combination of beta (1-3) glucan and itraconazole. However, the reproductive and fertility effects of this association were not known. So, the aim of this study was to identify the effects of the combination of itraconazole and beta (1-3) glucan, extracted from Saccharomyces cerevisiae, on male rats fertility. Materials, Methods & Results: Sixty male Wistar rats with 120-days-old were used. The experimental protocol was approved by Ethics Committee on Animal Use of the Federal University of Rio Grande do Sul (protocol CEUA/UFRGS no. 19452/2010). The animals were placed into six groups (n = 10 animals / group) as following: Negative Control group - treated daily with the volume corresponding to 10 mL.kg-¹ of sterile distilled water orally and 0.25 mL of sterile normal saline (NaCl 0.9 %) subcutaneously weekly; Itraconazole (IT) group - treated daily with itraconazole solution at a dose of 10 mg.kg-¹ orally and 0.25 mL of sterile 0.9% NaCl subcutaneously weekly; Beta group - treated daily with the volume corresponding to 10 mL.kg-¹ of sterile distilled water orally and 0.5 mg of beta (1-3) glucan subcutaneously weekly; Therapeutic Dose (TD) group - daily treated with itraconazole at a dose of 10 mg.kg-¹ orally and 0.5 mg of ß (1-3) glucan subcutaneously weekly; TD5x - treated daily with itraconazole at a dose of 50 mg.kg-¹ orally and 0.5 mg of beta (1-3 ) glucan subcutaneously weekly; TD10x - daily treated with itraconazole at a dose of 100 mg.kg-¹ orally and 0.5 mg of beta (1-3) glucan subcutaneously weekly. The rats were treated for 91 consecutive days. Individual body mass, organs relative mass and histopathology, number of sperm in the cauda epididymis, daily spermatozoal production, sperm parameters, sperm morphology, serum testosterone concentration and reproductive rates were evaluated. Significant differences were observed in daily spermatozoal production, sperm morphology, serum concentration of testosterone, mating rate and birth rate, with lower results in the TD5x and TD10x groups. Discussion: The systemic toxicity indicators, as body mass variation, water intake and clinical signs, as well as organ histology suggest that systemic toxicity in these animals did not occur. The decrease in serum testosterone concentrations in elevated doses of itraconazole associated with beta (1-3) glucan must be involved in decrease of sperm parameters and in sexual behavior and consequently, in the reproductive rates. Changes in sperm morphology, mainly found in sperm head, indicate sperm immaturity, preamature spermiation, abnormal or degenerate acrosome. Based on these results, it is concluded that beta (1-3) glucan and itraconazole did not affect the male rats reproductive variables when used in therapeutic doses alone or in combination, however these variables were altered with higher doses of itraconazole in the association. These data, added to the absence of histopathological damage of the testes suggest functional effect on male fertility. Caution is advised in the use of high doses of itraconazole with or without beta (1-3) glucan in males, especially in prolonged therapy.

Paniculite traumática em um cão / Traumatic panniculitis in a dog

Background: Panniculitis can be associated with a group of multifactorial diseases that develop intense inflammatory response of the subcutaneous fat tissue. The damaged fat cells suffer hydrolysis and saponification. The etiology remains unknown, although it has been associated with infectious agents, vascular pathologies, pancreatic disorders, neoplasms, immunologic diseases and nutritional deficiencies. Nodular sterile panniculitis is referred to the fat subcutaneous tissue inflammation without the presence of microorganisms and generally is presented with multiple or single nodules associated either with systemic or traumatic issues. This assay reports a case of traumatic Panniculitis presented as a single nodule on a dog admitted at the Veterinary Teaching Hospital of Passo Fundo University (HV-UPF), State of Rio Grande do Sul, Brazil, emphasizing the clinical signs, diagnosis and surgical treatment. Case: An adult mixed breed bitch, weighting 7,5 kg, was admitted at the HV-UPF because of a one-month evolution of a dorsal thoracic subcutaneous mass. Symptomatic treatment with unknown oral antibiotics was administered by another veterinarian, who also tried to perform local puncture drainage unsuccessfully. There was no previous history of local puncture or subcutaneous drug infiltration that could explain the mass. During physical examination, a single, painless and nodular subcutaneous mass (4 x 6 cm) was located on the dorsal thoracic median line, below the scapula, discharging a serosanguineous fluid probably due to the previous puncture. No other occurrences were found. The patient was treated with cephalexin and meloxicam. Hematological and biochemistry examinations were within reference range. Cytological exam demonstrated slightly degenerated neutrophilis and a great amount of adipose tissue. This could suggest, among other pathologies, neoplasm. Surgery was performed after 15 days. An elliptical incision was performed maintaining a 2,5 cm safety margin from the mass. Histopathological findings were compatible with sterile nodular panniculitis, likely traumatic. Discussion: There was a single, firm, regular, painless nodule, correspondent with other studies. The lesion location was also similar to other author's findings. Cytological findings were unspecific and could lead to several diagnoses. Careful pathologic study is necessary to differentiate from benign or malignant neoplasms and other affections. The hematological and biochemistry examinations, ultrasounds and radiographs were all within reference range on the case. These diagnose tools are very important and should always be used in order to achieve the correct screening, diagnosis and treatment. Surgical removal was considered effective, once 7 months after the treatment there was no local recurrence, with great cosmetic result.

Luxação lateral de patela em um Poodle toy / Lateral patellar luxation in Poodle toy

Luxating patella is one of the most frequent conditions of the stifle joint in dogs and it can evolve into a degenerative joint disease. The occurrence of the condition can be medial or lateral, unilateral or bilateral. Its origin can be traumatic or congenital and, additionally, it may undergo genetic and environmental influence. Lateral luxation is more common in large breed dogs, and it can rarely affect medium and small breeds, whilst medial luxation is more common in small breed dogs. The classification of patellar luxation comprises four grades, according to the patellar position, clinical signs and seriousness of the bone deformities found. The diagnosis is made based on the clinical evaluation, associated with a radiographic evaluation. The present study aims at describing a case of bilateral grade 3 lateral luxating patella in a Toy Poodle dog.

Luxação lateral de patela em um Poodle toy / Lateral patellar luxation in Poodle toy

Luxating patella is one of the most frequent conditions of the stifle joint in dogs and it can evolve into a degenerative joint disease. The occurrence of the condition can be medial or lateral, unilateral or bilateral. Its origin can be traumatic or congenital and, additionally, it may undergo genetic and environmental influence. Lateral luxation is more common in large breed dogs, and it can rarely affect medium and small breeds, whilst medial luxation is more common in small breed dogs. The classification of patellar luxation comprises four grades, according to the patellar position, clinical signs and seriousness of the bone deformities found. The diagnosis is made based on the clinical evaluation, associated with a radiographic evaluation. The present study aims at describing a case of bilateral grade 3 lateral luxating patella in a Toy Poodle dog.(AU)