Tungíase em cão de uma comunidade rural do estado do Maranhão / Tungíasis in a dog from a rural community in the state of Maranhão

A tungíase é uma infecção parasitária causada pela fêmea da pulga Tunga penetrans, que penetra na pele do hospedeiro para se alimentar e desenvolver seus ovos, gerando lesões que provocam prurido e dor. Assim, o objetivo deste trabalho foi relatar um caso de tungíase em um cão domiciliado em uma comunidade rural de Colinas, Maranhão. Em julho de 2020, um cachorro da raça American Pit Bull Terrier, com um ano e oito meses de idade, foi levado a uma clínica veterinária apresentando irritação e incômodo nas patas, o que dificultava sua locomoção. O cão vivia em uma residência com solo de chão batido, com acesso à rua e o tutor não possuía outros animais. Ao exame físico, constatou-se a presença de ectoparasitos nos coxins palmares e plantares, que foram retirados com auxílio de pinça cirúrgica e encaminhados ao Laboratório de Parasitologia da UFPI para identificação, confirmando tratar-se de pulgas da espécie Tunga penetrans. O cão recebeu tratamento à base de ivermectina 1%, 0,5mL via subcutânea, dose única, apresentando melhora no quadro clínico, e o tutor recebeu orientações sobre a desinfestação do ambiente. Conclui-se que a tungíase pode acometer cães em áreas rurais de um município do Maranhão, sendo importante o diagnóstico precoce e o tratamento adequado.

Tungiasis is a parasitic infection caused by the female flea Tunga penetrans, which penetrates the host's skin to feed and develop its eggs, generating lesions that provoke itching and pain. Thus, this study aimed to report a case of tungiasis in a dog domiciled in a rural community in Colinas, Maranhão. In july 2020, an American Pit Bull Terrier dog, one year and eight months old, was taken to a veterinary clinic with irritation and discomfort in the paws, which made it difficult to move. The dog lived in a house with beaten ground soil, had access to the street, and the tutor had no other animals. The physical examination showed the presence of ectoparasites on the palm and plantar pads, which were removed with surgical tweezers and sent to the UFPI Parasitology Laboratory for identification, confirming that they were Tunga penetrans fleas. The dog received a treatment based on ivermectin 1%, 0.5mL subcutaneously, single dose, showing improvement in the clinical picture, and the tutor received guidance on the disinfection of the environment. It is concluded that tungiasis can affect dogs in rural areas of a city in Maranhão, being important the early diagnosis and adequate treatment.

Efeito da adição do ácido palmítico e da vitamina E ao diluidor Tris-gema na criopreservação de sêmen canino / Effect of addition of palmitic acid and Vitamin E to the Tris-egg yolk diluter in canine semen cryopreservation

Canine sperm is a very delicate cell that is quite susceptible to oxidative stress since the cytoplasm is restricted and features little antioxidant reserves. Furthermore, the sperm membrane has some polyunsaturated fatty acids sensitive to lipid peroxidation, which makes it important to addition antioxidant substances to the diluter aiming at decreasing such stress to the sperm cell, particularly during seminal cryopreservation. Several antioxidants have been used in this process in some domestic animal's species, however, the use of palmitic acid has been little reported in works on cryopreservation of semen of the canine species. Hence, this study aimed to assess the effect of addition antioxidants palmitic acid and vitamin E to the Tris-egg yolk diluter on the semen quality of dogs after thawing. Samples were collected from the ejaculates of 4 adult dogs, apparently healthy, of the American Pit Bull Terrier breed of kennels in the city of Teresina, PI, places where the pre-freezing procedures of the dog's semen were performed. The samples were diluted in Tris citric acid fructose (3.28 g Tris-hydroxymethyl-aminomethane, 1.78 g citric acid monohydrate and 1.25 g D-fructose), dissolved in 100 mL distilled water, and added 20% egg yolk and 6% glycerol, at the concentration of 100x106 sptz/mL. The semen samples were divided into 3 mL aliquots to form 3 experimental groups: G1 - Only Tris-egg yolk (Control group); G2 - Tris-egg yolk + 100 µM palmitic acid; and G3 - Tris-egg yolk + 116 µM vitamin E. Semen was collected weekly over a period of little over 2 months. After thawing, thermorresistance test (TTR) was carried out at 0, 30, 60, and 90 min to assess spermatics motility and vigor, in addition to analysis of integrity of plasma membrane, acrosomal membrane and mitochondrial activity of the sperm, using fluorescent probes. These assessments were performed out at the Animal Reproduction Biotechnology Laboratory (LBRA/UFPI). In the TTR, G2 and G3 didn't exhibit significant results for spermatics motility or vigor when compared with the control group. The palmitic acid and vitamin E also had no significant effects on the parameters of acrosomal membrane integrity or mitochondrial activity. However, sperm cryopreserved with the addition of palmitic acid exhibited significant differences for plasma membrane integrity, providing greater protection to the sperm cells in G2. The palmitic acid is one of the most saturated fatty acids in human semen, with reports of great proportions also in the seminal plasma of dogs. Its main role is to protect the plasma membrane from external damage, improving viability and fertility of the sperm after cryopreservation. Data is scarce in the literature on the composition of fatty acids in canine semen and regarding the use of palmitic acid as a seminal antioxidant in that species, which grants further studies aiming to investigate such valuable information for canine reproduction. It is concluded that addition palmitic acid at 100µM concentration to the Tris-egg yolk diluter was able to preserve the integrity of the plasma membrane during the process of cryopreservation of canine semen.(AU)

Alterações clínicas, laboratoriais e seminais de cães naturalmente infectados por Leishmania sp / Clinical, laboratory and seminal changes of dogs naturally infected by Leishmania sp

A Leishmaniose Visceral (LV) é uma doença parasitária crônica, grave e endêmica em algumas regiões do Brasil, tendo os humanos e os cães como principais hospedeiros. Objetivou-se com este trabalho avaliar o perfil clínico, laboratorial e seminal de cães machos Sem Raça Definida (SRD) diagnosticados com LV, no município de Teresina, Piauí. Os animais foram provenientes da Gerência de Controle de Zoonoses do município e levados para o canil do Centro de Ciências Agrárias da Universidade Federal do Piauí, onde permaneceram de fevereiro a abril de 2014 sob avaliação e realização de exames. Foram coletadas amostras de sangue e sêmen de 12 cães, divididos em dois grupos, sendo 06 positivos (GI) e 06 negativos (GII) para a doença. Os resultados apontaram linfadenomegalia, emagrecimento e onicogrifose como principais sinais clínicos nos cães com LV e as alterações hematológicas foram, anemia normocítica normocrômica e trombocitopenia. A análise de bioquímica sérica revelou aumento nos índices de ureia nos animais parasitados no final do experimento, com diferença estatística entre os grupos, porém os níveis de creatinina mantiveram-se sem alteração. Quanto às análises seminais, foram encontradas diferenças significativas entre os grupos nos parâmetros de motilidade, vigor e concentração espermática, além da presença de patologias espermáticas que indicaram aumento de espermatozoides com alterações de cabeça e cauda. Conclui-se que cães com LV apresentam alterações clínicas, laboratoriais e seminais que podem prejudicar a condição geral e reprodutiva dessa espécie animal.

The Visceral Leishmaniasis (VL) is a chronic and severe parasitic disease, endemic in some regions of Brazil, and having dogs and humans as the main hosts. The objective of this work was to evaluate the clinical, laboratory and seminal profile of Mixed breed male dogs diagnosed with VL, in the city of Teresina, Piauí. The animals were obta ined from the Zoonoses Control Management of city and taken to the kennel of the Agrarian Sciences Center of the Federal University of Piauí, where they remained from February to April 2014 under evaluation and exams. Blood and semen samples were collected from 12 dogs, divided into two groups, being 06 positive (GI) and 06 negative (GII) for the disease. The results indicated lymphadenomegaly, weight loss and onychogryphosis as the main clinical signs in dogs with VL and the hematological changes were normochromic normocytic anemia and thrombocytopenia. The analysis of serum biochemistry revealed an increase in urea indexes in the parasitized animals at the end of the experiment, with statistical difference between the groups, however the levels of creatinine remained unchanged. Regarding the seminal analyzes, significant differences were found between the groups in the parameters of motility, vigor and sperm concentration, in addition to the presence of sperm pathologies that indicated an increase in sperm with changes of the head and tail. It is concluded that dogs with VL present clinical, laboratory and seminal alterations that can impair the general and reproductive condition of this animal species.

Alterações clínicas, laboratoriais e seminais de cães naturalmente infectados por Leishmania sp / Clinical, laboratory and seminal changes of dogs naturally infected by Leishmania sp

A Leishmaniose Visceral (LV) é uma doença parasitária crônica, grave e endêmica em algumas regiões do Brasil, tendo os humanos e os cães como principais hospedeiros. Objetivou-se com este trabalho avaliar o perfil clínico, laboratorial e seminal de cães machos Sem Raça Definida (SRD) diagnosticados com LV, no município de Teresina, Piauí. Os animais foram provenientes da Gerência de Controle de Zoonoses do município e levados para o canil do Centro de Ciências Agrárias da Universidade Federal do Piauí, onde permaneceram de fevereiro a abril de 2014 sob avaliação e realização de exames. Foram coletadas amostras de sangue e sêmen de 12 cães, divididos em dois grupos, sendo 06 positivos (GI) e 06 negativos (GII) para a doença. Os resultados apontaram linfadenomegalia, emagrecimento e onicogrifose como principais sinais clínicos nos cães com LV e as alterações hematológicas foram, anemia normocítica normocrômica e trombocitopenia. A análise de bioquímica sérica revelou aumento nos índices de ureia nos animais parasitados no final do experimento, com diferença estatística entre os grupos, porém os níveis de creatinina mantiveram-se sem alteração. Quanto às análises seminais, foram encontradas diferenças significativas entre os grupos nos parâmetros de motilidade, vigor e concentração espermática, além da presença de patologias espermáticas que indicaram aumento de espermatozoides com alterações de cabeça e cauda. Conclui-se que cães com LV apresentam alterações clínicas, laboratoriais e seminais que podem prejudicar a condição geral e reprodutiva dessa espécie animal.(AU)

The Visceral Leishmaniasis (VL) is a chronic and severe parasitic disease, endemic in some regions of Brazil, and having dogs and humans as the main hosts. The objective of this work was to evaluate the clinical, laboratory and seminal profile of Mixed breed male dogs diagnosed with VL, in the city of Teresina, Piauí. The animals were obta ined from the Zoonoses Control Management of city and taken to the kennel of the Agrarian Sciences Center of the Federal University of Piauí, where they remained from February to April 2014 under evaluation and exams. Blood and semen samples were collected from 12 dogs, divided into two groups, being 06 positive (GI) and 06 negative (GII) for the disease. The results indicated lymphadenomegaly, weight loss and onychogryphosis as the main clinical signs in dogs with VL and the hematological changes were normochromic normocytic anemia and thrombocytopenia. The analysis of serum biochemistry revealed an increase in urea indexes in the parasitized animals at the end of the experiment, with statistical difference between the groups, however the levels of creatinine remained unchanged. Regarding the seminal analyzes, significant differences were found between the groups in the parameters of motility, vigor and sperm concentration, in addition to the presence of sperm pathologies that indicated an increase in sperm with changes of the head and tail. It is concluded that dogs with VL present clinical, laboratory and seminal alterations that can impair the general and reproductive condition of this animal species.(AU)

Testicular and seminal evaluation of dogs naturally infected with Leishmania sp / Avaliação testicular e seminal de cães naturalmente infectados por Leishmania sp

The objective of this study was to evaluate the testicular changes and detect the presence of Leishmania sp. in the testicles and semen of dogs with Visceral Leishmaniasis (VL). The animals were obtained from the Zoonoses Control Department of Teresina, PI, and taken to the kennel of the Agricultural Sciences Center of the Federal University of Piauí, where they remained were maintained for two months and subsequently euthanized for testicles removal. Semen samples were collected from 12 dogs, 06 positive and 06 negative for VL. The following diagnostic techniques readouts were assessed for the sampled animals: testosterone dosage, immunohistochemistry (IMH), histopathology of the slides containing the testicular material, and seminal evaluation by polymerase chain reaction (PCR). Testosterone values remained within the normal range for the canine specie and did not differ statistically among the experimental groups but displayed lower serum concentrations than those of the control group. All the testicular and semen samples from the dogs were negative for VL as determined by techniques IMH and PCR, respectively. The results of testicle histopathology revealed the presence of several lesions with statistical difference among the experimental groups. Parasitized dogs with VL have testicular lesions that may compromise the reproductive efficiency of these animals.(AU)

O objetivo deste estudo foi avaliar as alterações testiculares e detectar a presença de Leishmania sp. nos testículos e sêmen de cães com leishmaniose visceral (LV). Os animais foram obtidos da Gerência de Controle de Zoonoses de Teresina, PI, e levados ao canil do Centro de Ciências Agrárias da Universidade Federal do Piauí, onde permaneceram por dois meses e posteriormente foram submetidos à eutanásia para a remoção dos testículos. As amostras de sêmen foram coletadas de 12 cães, sendo 06 positivos e 06 negativos para LV. Nas amostras dos animais, foram realizadas as seguintes técnicas de diagnóstico: dosagem de testosterona, imunohistoquímica (IMH), histopatologia das lâminas contendo o material testicular e avaliação seminal por reação em cadeia pela polimerase (PCR). Valores de testosterona permaneceram dentro do intervalo normal para a espécie canina, não diferindo estatisticamente entre os grupos experimentais, porém apresentando concentrações séricas mais baixas do que o grupo controle. Todas as amostras testiculares e de sêmen dos cães foram negativas para LV, conforme determinado pelas técnicas IMH e PCR, respectivamente. Os resultados da histopatologia dos testículos revelaram a presença de várias lesões com diferença estatística entre os grupos experimentais. Os cães parasitados com LV apresentam lesões testiculares que podem comprometer a eficiência reprodutiva desses animais.(AU)

Avaliação laboratorial e seminal de um cão pós-tratamento quimioterápico para tumor venéreo transmissível: relato de caso

Este trabalho relata um caso de um cão vadio, sem raça definida (SRD), capturado pela Gerência de Controle de Zoonoses de Teresina, PI e levado para o canil da Universidade Federal do Piauí. Após avaliação clínica e citológica, o animal foi diagnosticado com tumor venéreo transmissível (TVT) no pênis e tratado com sulfato de vincristina em sessões semanais com remissão dos nódulos. Após a última sessão de quimioterapia foram realizados hemograma e avaliação seminal com resultados dentro dos parâmetros de normalidade para a espécie canina. Foi possível observar algumas patologias espermáticas, porém sem comprometimento reprodutivo do animal. Portanto, conclui-se que cães tratados para TVT podem ser imediatamente reintroduzidos à reprodução.(AU)

This paper reports a case of a stray dog, breed undefined, captured by the Department Control of Zoonoses of Teresina, PI and taken to the Federal University of Piauí kennel. After clinical and cytological evaluation, the animal was diagnosed with transmissible venereal tumor (TVT) in the penis and treated with vincristine sulfate in weekly sessions with remission of the nodules. Hemogram and semen evaluation with results within the parameters of normality for the canine specie were performed after the last chemotherapy session. It was possible to observe some spermatic pathologies, but without reproductive impairment of the animal. Therefore, it is concluded that dogs treated for TVT can be immediately reintroduced for reproduction.(AU)

Avaliação laboratorial e seminal de um cão pós-tratamento quimioterápico para tumor venéreo transmissível: relato de caso

Este trabalho relata um caso de um cão vadio, sem raça definida (SRD), capturado pela Gerência de Controle de Zoonoses de Teresina, PI e levado para o canil da Universidade Federal do Piauí. Após avaliação clínica e citológica, o animal foi diagnosticado com tumor venéreo transmissível (TVT) no pênis e tratado com sulfato de vincristina em sessões semanais com remissão dos nódulos. Após a última sessão de quimioterapia foram realizados hemograma e avaliação seminal com resultados dentro dos parâmetros de normalidade para a espécie canina. Foi possível observar algumas patologias espermáticas, porém sem comprometimento reprodutivo do animal. Portanto, conclui-se que cães tratados para TVT podem ser imediatamente reintroduzidos à reprodução.

This paper reports a case of a stray dog, breed undefined, captured by the Department Control of Zoonoses of Teresina, PI and taken to the Federal University of Piauí kennel. After clinical and cytological evaluation, the animal was diagnosed with transmissible venereal tumor (TVT) in the penis and treated with vincristine sulfate in weekly sessions with remission of the nodules. Hemogram and semen evaluation with results within the parameters of normality for the canine specie were performed after the last chemotherapy session. It was possible to observe some spermatic pathologies, but without reproductive impairment of the animal. Therefore, it is concluded that dogs treated for TVT can be immediately reintroduced for reproduction.

Trypanosoma vivax em bovino leiteiro / Trypanosoma vivax in dairy cattle

Background: Trypanosoma vivax is a protozoan that causes reproductive disorders and decreased production in domestic and wild ungulate animals. The bovine are the main hosts of the disease and the transmission occurs by the bite of hematophagous insects, mainly tabanids. Several diagnostic techniques can be used to detect the parasite, both in parasitological form and by serological kits. In Brazil, the disease has been reported in bovines, goats and sheep of some states, with high morbidity and mortality and due to the scarcity of results on the epidemiology of the disease, this work had the objective to report the presence of T. vivax in a female bovine of a dairy herd in Parnaíba county, Piauí.Case: The animal naturally infected by Trypanosoma vivax, was a three-year-old cow from a dairy farm in the Parnaíba county, located in the north of Piauí state. The farm had a herd whith 62.20% of young Girolando breed cows and the breeding system used was semi-confinement, with two mechanical milking per day. At the time of a Veterinarian’s technical visit to the property, it was observed the occurrence of abortions, mastitis, estrus repetitions and cows with hematuria, leading to the suspicion of the bovine leptospirosis occurrence. Blood samples were collected from 78 cows from the herd for hematological, biochemical and serological tests, and 72 (92.30%) were reactive to some Leptospira serovars. All the exams were carried out at the Federal University of Piauí (UFPI). In the group of animals negative for leptospirosis, a female was diagnosed positive for bovine trypanosomiasis, confirming the result in the blood smear. This animal had no clinical signs characteristic of the disease at the time of the evaluation.[...]

Trypanosoma vivax em bovino leiteiro / Trypanosoma vivax in dairy cattle

Background: Trypanosoma vivax is a protozoan that causes reproductive disorders and decreased production in domestic and wild ungulate animals. The bovine are the main hosts of the disease and the transmission occurs by the bite of hematophagous insects, mainly tabanids. Several diagnostic techniques can be used to detect the parasite, both in parasitological form and by serological kits. In Brazil, the disease has been reported in bovines, goats and sheep of some states, with high morbidity and mortality and due to the scarcity of results on the epidemiology of the disease, this work had the objective to report the presence of T. vivax in a female bovine of a dairy herd in Parnaíba county, Piauí.Case: The animal naturally infected by Trypanosoma vivax, was a three-year-old cow from a dairy farm in the Parnaíba county, located in the north of Piauí state. The farm had a herd whith 62.20% of young Girolando breed cows and the breeding system used was semi-confinement, with two mechanical milking per day. At the time of a Veterinarians technical visit to the property, it was observed the occurrence of abortions, mastitis, estrus repetitions and cows with hematuria, leading to the suspicion of the bovine leptospirosis occurrence. Blood samples were collected from 78 cows from the herd for hematological, biochemical and serological tests, and 72 (92.30%) were reactive to some Leptospira serovars. All the exams were carried out at the Federal University of Piauí (UFPI). In the group of animals negative for leptospirosis, a female was diagnosed positive for bovine trypanosomiasis, confirming the result in the blood smear. This animal had no clinical signs characteristic of the disease at the time of the evaluation.[...](AU)

Parto prematuro em cadela com Leishmaniose Visceral: relato de caso / Premature parturition in a bitch with Visceral Leishmaniasis: a case report

This paper reports a Schnauzer breed bitch, two years and six months old, pregnant, who was diagnosedwith Visceral Leishmaniasis (LV), and its clinical and laboratory findings aggravated during pregnancy with theoccurrence of premature parturition at fifty days of coverage. It is worth mentioning that bitches with LV should beexcluded from reproduction because the disease may change the overall clinical picture of the animal and implicatethe development of the fetus, causing miscarriage or premature parturition with stillbirths.(AU)

Avaliação seminal de cão com Tumor Venéreo Transmissível após tratamento quimioterápico: relato de caso / Seminal evaluation of dog with Transmissible Venereal Tumor after chemotherapy: a case report

This paper reports a stray dog, SRD, with about three years old, collected by the Zoonosis ControlManagement Teresina, PI and taken to the kennel of Piauí Federal University with transmissible venereal tumoron the glans penis. After clinical evaluation, the animal was treated with vincristine sulfate in three weeklysessions, with complete remission of the nodules and semen collection was later performed for evaluation. Theresults of the parameters of volume, motility, vigor and sperm concentration were normal for dogs. Spermpathologies were revealed in 36% larger defects and 12% of minor defects. The test of fluorescent probes, 35%of the sperm plasma membranes were intact and 54% of acrosomal membranes and a 48% mitoncondrialpotential, revealing that after chemotherapy, sperm structures are maintained with few functional alterationsbeing possible reentry dog for reproduction.(AU)

Avaliação seminal de cão com Tumor Venéreo Transmissível após tratamento quimioterápico: relato de caso / Seminal evaluation of dog with Transmissible Venereal Tumor after chemotherapy: a case report

This paper reports a stray dog, SRD, with about three years old, collected by the Zoonosis ControlManagement Teresina, PI and taken to the kennel of Piauí Federal University with transmissible venereal tumoron the glans penis. After clinical evaluation, the animal was treated with vincristine sulfate in three weeklysessions, with complete remission of the nodules and semen collection was later performed for evaluation. Theresults of the parameters of volume, motility, vigor and sperm concentration were normal for dogs. Spermpathologies were revealed in 36% larger defects and 12% of minor defects. The test of fluorescent probes, 35%of the sperm plasma membranes were intact and 54% of acrosomal membranes and a 48% mitoncondrialpotential, revealing that after chemotherapy, sperm structures are maintained with few functional alterationsbeing possible reentry dog for reproduction.

Parto prematuro em cadela com Leishmaniose Visceral: relato de caso / Premature parturition in a bitch with Visceral Leishmaniasis: a case report

This paper reports a Schnauzer breed bitch, two years and six months old, pregnant, who was diagnosedwith Visceral Leishmaniasis (LV), and its clinical and laboratory findings aggravated during pregnancy with theoccurrence of premature parturition at fifty days of coverage. It is worth mentioning that bitches with LV should beexcluded from reproduction because the disease may change the overall clinical picture of the animal and implicatethe development of the fetus, causing miscarriage or premature parturition with stillbirths.

Caracterização física e estrutural de espermatozoides no ejaculado de Caprinos Azul, Canindé, Moxotó e Repartido / Physical and structural characterization of sperm in the ejaculate from Azul, Caninde, Moxotó and Repartido goat races

This study aimed to evaluate physically and structurally ejaculates from locally adapted goats in therainy season. Semen pool formed by the four goats of each breed was evaluated for physical, diluted in Tris-eggyolk and frozen. After thawing, it was diluted in Tris and incubated with carboxyfluorescein diacetate andpropidium iodide, to evaluate the integrity of the plasma membrane and JC1 to assess mitochondrial activity.The percentage of cells with damaged membrane and mitochondrial activity in spermatozoa between breed werecompared, every hour for three hours. There was no significant difference (P > 0,05) in relation to thepercentage of cells with damaged plasma membrane, as well as between the incubation times. The Azul breedhad lower mitochondrial activity (P < 0,05). The evaluated semen of all breeds showed no significant damage tothe plasma membrane in the rainy season, but the Azul breed had lower mitochondrial activity in sperm cells.(AU)

Avaliação do chá do extrato de copaíba (Copaifera luetzelburgii) pós-criopreservação de sêmen de caprinos pelo teste de termoresistência / Evaluation of the tea of copaíba extract (Copaifera luetzelburgii) in goat semen post-thawing by term-resistance test

The semen cryopreservation process causes damages in spermatozoa characteristics. This study aimedto evaluate the effects of tea of Copaíba extract (Copaifera luetzelburgii), in different concentrations, added tothe Tris-egg yolk extender, in the quality of post-cryopreserved spermatozoa from locally adapted breeds ofgoats. Were used four (4) goats Breeds (Azul, Canindé, Moxotó e Repartido) adults, male, six of each breed,aged between 1 and 6 years, a pool of ejaculates were formed to analyze. The animals were randomly dividedinto four experimental treatments: TI (Control), TII (0,1mg), TIII (0,2mg) and TIV (0,5mg). The samples werefrozen and stored, after 30 days they were thawed for analysis by term-resistance test (TTR). The addition of teaof copaíba extract showed no significant difference between treatments by TTR, therefore the use of tea copaíbaextract in seminal diluting not increment the sperm longevity post-thawing.(AU)

Avaliação morfológica, funcional e tempo de termorresistência de espermatozoides caprinos localmente adaptados / Morphological, functional and thermoresistance time evaluation of locally adapted goats sperm

This study evaluated the quality of ejaculated from adapted breeds of goats as the morphologic aspects,functional and thermoresistance. An aliquot of the pool of each breed was used for morphological evaluation bywet preparation, quantifying sperm defects and dividing them into classes. The samples were cryopreservedusing TK3000 machine and evaluated by thermoresistance test by checking motility and sperm vigor every hour.For plasma membrane functionality analysis, was used hiposmotic test. There was no difference (P > 0.05)between the breeds in all sperm defects before and after cryopreservation, as well as for the thermoresistancetimes for motility and sperm vigor (P > 0.05). There was a significant reduction in motility and sperm vigor 120minutes after thawing of the samples. There was no difference (P > 0.05) between the percentage of sperm withfunctional membrane between the evaluated breeds. Therefore, the evaluated breeds have morphology,functionality and similar sperm thermoresistance.(AU)

Avaliação do chá do extrato de copaíba (Copaifera luetzelburgii) adicionado ao diluidor Tris-gema na funcionalidade da membrana plasmática de espermatozoides caprinos criopreservados / Evaluation the copaíba tea extract (Copaifera luetzelburgii) added to Tris-yolk extender in the functionality of the plasma membrane of cryopreserved goats sperm

This study aims to evaluate the effects of tea of copaíba extract (Copaifera luetzelburgii), in differentconcentrations, added to the Tris-egg yolk extender, the functionality of the plasma membrane sperm ofcryopreserved goats. Were used four goats breeds, adults, male, six of each breed, aged between 1 and 6 years,a pool of ejaculates were formed to analyze. The animals were randomly divided into four experimentaltreatments: TI without adding of tea of copaíba extract (control); TII, added 0,1 mg of tea of copaíba extract;TIII, 0,2 mg; and TIV, 0,5 mg. The samples were frozen and stored, after 30 days were thawed for analysis byhisposmotic test (HOST). Adding tea of copaíba extract showed no significant difference between treatments forHOST, so the use of tea of copaíba extract the seminal dilution was not effective in preserving the functionalityplasma membrane sperm of goats post-thawing.(AU)

Histopatologia e Imunohistoquímica de testículos de cães naturalmente infectados por Leishmania sp / Histopathology and Immunohistochemistry testicles of dogs naturally infected by Leishmania sp

The objective of this study was to evaluate testicular changes and the presence of Leishmania sp. intesticles of dogs with Visceral Leishmaniasis (LV). The animals were obtained from Teresina Zoonosis ControlAdministration, and taken to the kennels of Agricultural Sciences Center of the Federal University of Piauí(UFPI), where they stayed for two months and were subsequently euthanized for removal of the testicles. 12 dogswere used, 06 positive (GI) and 06 negative (GII) for LV and conducted examinations of histopathology andimmunohistochemistry (IMH) of the testicles. All were negative by IMH technique and by histopathology. In thisstudy, we observed testicular lesions in all animals, with no direct relationship between the presence ofLeishmania sp. and intensity of the damage found in the reproductive system, reducing the possibility of venerealtransmission between oligositomatics dogs.(AU)

Avaliação do chá do extrato de copaíba (Copaifera luetzelburgii) adicionado ao diluidor Tris-gema na funcionalidade da membrana plasmática de espermatozoides caprinos criopreservados / Evaluation the copaíba tea extract (Copaifera luetzelburgii) added to Tris-yolk extender in the functionality of the plasma membrane of cryopreserved goats sperm

This study aims to evaluate the effects of tea of copaíba extract (Copaifera luetzelburgii), in differentconcentrations, added to the Tris-egg yolk extender, the functionality of the plasma membrane sperm ofcryopreserved goats. Were used four goats breeds, adults, male, six of each breed, aged between 1 and 6 years,a pool of ejaculates were formed to analyze. The animals were randomly divided into four experimentaltreatments: TI without adding of tea of copaíba extract (control); TII, added 0,1 mg of tea of copaíba extract;TIII, 0,2 mg; and TIV, 0,5 mg. The samples were frozen and stored, after 30 days were thawed for analysis byhisposmotic test (HOST). Adding tea of copaíba extract showed no significant difference between treatments forHOST, so the use of tea of copaíba extract the seminal dilution was not effective in preserving the functionalityplasma membrane sperm of goats post-thawing.

Avaliação do chá do extrato de copaíba (Copaifera luetzelburgii) pós-criopreservação de sêmen de caprinos pelo teste de termoresistência / Evaluation of the tea of copaíba extract (Copaifera luetzelburgii) in goat semen post-thawing by term-resistance test

The semen cryopreservation process causes damages in spermatozoa characteristics. This study aimedto evaluate the effects of tea of Copaíba extract (Copaifera luetzelburgii), in different concentrations, added tothe Tris-egg yolk extender, in the quality of post-cryopreserved spermatozoa from locally adapted breeds ofgoats. Were used four (4) goats Breeds (Azul, Canindé, Moxotó e Repartido) adults, male, six of each breed,aged between 1 and 6 years, a pool of ejaculates were formed to analyze. The animals were randomly dividedinto four experimental treatments: TI (Control), TII (0,1mg), TIII (0,2mg) and TIV (0,5mg). The samples werefrozen and stored, after 30 days they were thawed for analysis by term-resistance test (TTR). The addition of teaof copaíba extract showed no significant difference between treatments by TTR, therefore the use of tea copaíbaextract in seminal diluting not increment the sperm longevity post-thawing.