Clinical and nutritional follow-up of cats with chronic kidney disease fed with a renal prescription diet

Background: The use of prescription diets for cats with chronic kidney disease (CKD) is one of the main managementapproach of this disease in cats, and is considered a renoprotective strategy that may promote increased survival and/orimprove quality of life, according to the stage of CKD. Besides that, nutritional assessment is important to monitor themaintenance of quality of life of the patients and their response to disease, especially those with chronic conditions. Theaim of this study was to follow the clinical and nutritional status of cats with chronic kidney disease (CKD) IRIS stagesII, III and IV fed with a renal prescription diet, followed for 12 months.Materials, Methods & Results: Patients were fed exclusively with a dry renal prescription diet and medications for themanagement of CKD were prescribed when needed. Exclusion criteria were cats that already received a renal prescriptiondiet or medications for the treatment of CKD. Cats were evaluated every 2 months, considering body weight (BW), bodycondition score (BCS), muscle mass score (MMS), clinical and laboratory parameters. In all assessments, a complete bloodcount and biochemistry were performed by conventional methods with the patient fasted for 12 h. In addition, urinalysis,urine protein:creatinine ratio (UPC) and urine culture were performed from a urine sample collected by cystocentesis.The quantitative variables were tested for their stability on consecutive assessments using the non-parametric Friedmantest, and did not present significant variation during follow-up, except for systolic blood pressure (SBP). Eight cats witha diagnosis of CKD were included in the study and 6 of them remained in the same CKD stage during follow-up. On catdied due to an unrelated CKD cause. Regarding nutritional assessment, 5 of 7 cats maintained BW during the 12 months...

Clinical and nutritional follow-up of cats with chronic kidney disease fed with a renal prescription diet

Background: The use of prescription diets for cats with chronic kidney disease (CKD) is one of the main managementapproach of this disease in cats, and is considered a renoprotective strategy that may promote increased survival and/orimprove quality of life, according to the stage of CKD. Besides that, nutritional assessment is important to monitor themaintenance of quality of life of the patients and their response to disease, especially those with chronic conditions. Theaim of this study was to follow the clinical and nutritional status of cats with chronic kidney disease (CKD) IRIS stagesII, III and IV fed with a renal prescription diet, followed for 12 months.Materials, Methods & Results: Patients were fed exclusively with a dry renal prescription diet and medications for themanagement of CKD were prescribed when needed. Exclusion criteria were cats that already received a renal prescriptiondiet or medications for the treatment of CKD. Cats were evaluated every 2 months, considering body weight (BW), bodycondition score (BCS), muscle mass score (MMS), clinical and laboratory parameters. In all assessments, a complete bloodcount and biochemistry were performed by conventional methods with the patient fasted for 12 h. In addition, urinalysis,urine protein:creatinine ratio (UPC) and urine culture were performed from a urine sample collected by cystocentesis.The quantitative variables were tested for their stability on consecutive assessments using the non-parametric Friedmantest, and did not present significant variation during follow-up, except for systolic blood pressure (SBP). Eight cats witha diagnosis of CKD were included in the study and 6 of them remained in the same CKD stage during follow-up. On catdied due to an unrelated CKD cause. Regarding nutritional assessment, 5 of 7 cats maintained BW during the 12 months...(AU)

Association of polypropylene scaffolds and mesenchymal stem cells for use in tissue engineering / Associação de arcabouço de polipropileno e células tronco mesenquimais para uso em engenharia de tecido

Tissue engineering replaces injured tissues by manipulating cells, making scaffolds, and using molecules that stimulate the tissue. Mesenchymal stem cells (MSCs) are good candidates for tissue engineering, as this is one of the cell types which are recruited to repair injured tissues. Scaffolds are structural devices that allow cell fixation and migration, with polypropylene meshes being an example. This study aims to evaluate the culture of adipose tissue-derived mesenchymal stem cells (ADSCs), isolated from C57Bl/6 GFP + mice, in two types of polypropylene meshes (macroporous and microporous) in conventional culture plates and plates coated with methacrylate, over a period of fifteen days. The objective was to obtain the best interaction protocol between the mesh and the cells. The choice of the best method was based on adherence, maintenance of adherence and viability during culture. The amount of ADSCs adhering was checked daily by counting in a Neubauer Chamber and by using a growth curve performed with the MTT assay. The ADSCs adhering to the meshes were visualized with DAPI, panotic, hematoxylin and eosin, immunohistochemistry (integrin), and immunofluorescence (actin). ADSCs adhere to all forms of culture and to the two types of polypropylene mesh. ADSCs adhered more to the microporous mesh, within the seven day period of culture and in the plates without methacrylate. Thus, polypropylene meshes offer a good scaffold for ADSCs to adhere to.

A engenharia de tecidos substitui tecidos danificados com a manipulação de células, confecção de arcabouços e a utilização de moléculas que estimulem o tecido. As células-tronco mesenquimais (MSCs) são boas candidatas para engenharia de tecido, pois são um dos tipos celulares recrutadas para a reparação de tecidos lesionados. O arcabouço deve ser um dispositivo estrutural que forneça uma estrutura para o crescimento e a diferenciação celular no sítio, sendo a tela de polipropileno um exemplo. O objetivo deste estudo foi avaliar o cultivo de células-tronco mesenquimais de tecido de adiposo (ADSCs), isoladas de camundongos C57Bl/6 GFP+, em dois tipos de telas de polipropileno (macroporosa e microporosa) em placas de cultura convencionais e revestidas com metacrilato, durante quinze dias, para obter o melhor protocolo de interação entre a tela e as células. A escolha do melhor método foi baseada na adesão, manutenção da adesão e viabilidade durante cultivo. A quantidade de ADSCs aderidas foi verificada diariamente em contagem em Câmara de Neubauer e através de uma curva de crescimento realizada através de ensaio de MTT. As ADSCs aderidas nas telas foram visualizadas com a marcação de DAPI, panótico, hematoxilina e eosina, imumo-histoquímica (integrina) e imunofluorescência (actina). Nas duas formas de cultivo e nos dois tipos de telas de polipropileno houve aderência das ADSCs. Houve maior aderência na tela microporosa, no período de sete dias de cultivo e em placas sem metacrilato. Conclui-se que a tela de polipropileno oferece um bom arcabouço para as ADSCs se aderirem.

Associação de arcabouço de polipropileno e células tronco mesenqimais para uso em engenharia de tecido / Association of polypropylene scaffolds and mesenchymal stem cells for use in tissue engineering

A engenharia de tecidos tem como objetivo substituir tecidos danificados, manipulando células, confecção de arcabouços e a utilização de moléculas que estimulem o tecido. A proposta deste estudo foi avaliar duas técnicas de cultivo de células-tronco mesenquimais (MSC) em diferentes placas de cultura, utilizando dois tipos de telas de polipropileno (macroporoso e microporoso), para obter as melhores condições de interação entre a tela e as células, e definir uma proposta de protético para engenharia de tecidos. As telas de polipropileno foram cultivadas com células-tronco mesenquimais de tecido adiposo (ADSCs) isoladas de camundongos C57B1/6 GFP+ durante quinze dias em placas revestidas com metacrilato ou não revestidas com metacrilato. A quantidade de ADSCs aderidas foram verificadas diariamente em Câmara de Neubauer e através de uma curva de crescimento realizada pelo ensaio MTT. As ADSCs aderidas às malhas foram visualizadas com marcação de DAPI, panóticas, hematoxilina e eosina imuno-histoquímica e imunofluorescência. O melhor protocolo foi na tela microporosa, no o período de sete dias de cultivo e em placas sem metacrilato. Conclui-se que a tela de polipropileno fornece um bom suporte para as ADSCs se aderirem podendo ser utilizada na engenharia de tecidos.(AU)

Tissue engineering replaces injured tissues by manipulating cells, making scaffolds, and using molecules that stimulate the tissue. Mesenchymal stem cells (MSCs) are good candidates for tissue engineering, as this is one of the cell types which are recruited to repair injured tissues. Scaffolds are structural devices that allow cell fixation and migration, with polypropylene meshes being an example. This study aims to evaluate the culture of adipose tissue-derived mesenchymal stem cells (ADSCs), isolated from C57Bl/6 GFP + mice, in two types of polypropylene meshes (macroporous and microporous) in conventional culture plates and plates coated with methacrylate, over a period of fifteen days. The objective was to obtain the best interaction protocol between the mesh and the cells. The choice of the best method was based on adherence, maintenance of adherence and viability during culture. The amount of ADSCs adhering was checked daily by counting in a Neubauer Chamber and by using a growth curve performed with the MTT assay. The ADSCs adhering to the meshes were visualized with DAPI, panotic, hematoxylin and eosin, immunohistochemistry (integrin), and immunofluorescence (actin). ADSCs adhere to all forms of culture and to the two types of polypropylene mesh. ADSCs adhered more to the microporous mesh, within the seven day period of culture and in the plates without methacrylate. Thus, polypropylene meshes offer a good scaffold for ADSCs to adhere to.(AU)

Associação de arcabouço de polipropileno e células tronco mesenqimais para uso em engenharia de tecido / Association of polypropylene scaffolds and mesenchymal stem cells for use in tissue engineering

A engenharia de tecidos tem como objetivo substituir tecidos danificados, manipulando células, confecção de arcabouços e a utilização de moléculas que estimulem o tecido. A proposta deste estudo foi avaliar duas técnicas de cultivo de células-tronco mesenquimais (MSC) em diferentes placas de cultura, utilizando dois tipos de telas de polipropileno (macroporoso e microporoso), para obter as melhores condições de interação entre a tela e as células, e definir uma proposta de protético para engenharia de tecidos. As telas de polipropileno foram cultivadas com células-tronco mesenquimais de tecido adiposo (ADSCs) isoladas de camundongos C57B1/6 GFP+ durante quinze dias em placas revestidas com metacrilato ou não revestidas com metacrilato. A quantidade de ADSCs aderidas foram verificadas diariamente em Câmara de Neubauer e através de uma curva de crescimento realizada pelo ensaio MTT. As ADSCs aderidas às malhas foram visualizadas com marcação de DAPI, panóticas, hematoxilina e eosina imuno-histoquímica e imunofluorescência. O melhor protocolo foi na tela microporosa, no o período de sete dias de cultivo e em placas sem metacrilato. Conclui-se que a tela de polipropileno fornece um bom suporte para as ADSCs se aderirem podendo ser utilizada na engenharia de tecidos.

Tissue engineering replaces injured tissues by manipulating cells, making scaffolds, and using molecules that stimulate the tissue. Mesenchymal stem cells (MSCs) are good candidates for tissue engineering, as this is one of the cell types which are recruited to repair injured tissues. Scaffolds are structural devices that allow cell fixation and migration, with polypropylene meshes being an example. This study aims to evaluate the culture of adipose tissue-derived mesenchymal stem cells (ADSCs), isolated from C57Bl/6 GFP + mice, in two types of polypropylene meshes (macroporous and microporous) in conventional culture plates and plates coated with methacrylate, over a period of fifteen days. The objective was to obtain the best interaction protocol between the mesh and the cells. The choice of the best method was based on adherence, maintenance of adherence and viability during culture. The amount of ADSCs adhering was checked daily by counting in a Neubauer Chamber and by using a growth curve performed with the MTT assay. The ADSCs adhering to the meshes were visualized with DAPI, panotic, hematoxylin and eosin, immunohistochemistry (integrin), and immunofluorescence (actin). ADSCs adhere to all forms of culture and to the two types of polypropylene mesh. ADSCs adhered more to the microporous mesh, within the seven day period of culture and in the plates without methacrylate. Thus, polypropylene meshes offer a good scaffold for ADSCs to adhere to.

Treatment of canine multicentric lymphoma through vascular access port vs. peripheral venous catheter

Background: Vascular access port (VAP) was developed for the administration of chemotherapeutic agents, minimizinglocal drug reactions and complications associated with migration of peripheral venous catheter (PVC) in humans. Thedevice is widely used in human oncology and has gained importance in veterinary oncology, especially in long treatmentregimens, as in the case of canine lymphoma. VAP favors therapy and the animals life quality. The aim of this study wasto describe the use of VAP in dogs, comparing to PVC access, during canine lymphoma chemotherapeutic treatment.Materials, Methods & Results: Eleven dogs with multicentric lymphoma which required chemotherapy were selectedfor the study. The dogs were randomly allocated to two groups with five and six animals, and each group received thechemotherapy protocol through the PVC (n= 5) or VAP (n= 6). For the sake of standardization, assessments were madewhenever the dogs received vincristine sulfate, despite the use of the infusion system in all sessions of the MadisonWisconsin protocol. A VAP was implanted into the right external jugular vein of six dogs under inhalational anesthesia,using the Seldinger technique. Systolic blood pressure (SBP) levels and handling time during chemotherapy sessions werecompared in both groups in three time periods during the procedures: 10 min after arrival to each chemotherapy (P1);immediately after placement of the PVC or puncture of the VAP reservoir (P2); and at the end of chemotherapy (P3). Thearithmetic mean of five consecutive assessments was used in each time period. In the chemotherapy sessions, the mean ofSBP variation decreased statistically significant in the VAP group compared to PVC group. SBP decreased from P1 to P2and from P1 to P3 in all sessions (S1, S2, and S3) in the VAP group, and increased in the PVC...

Treatment of canine multicentric lymphoma through vascular access port vs. peripheral venous catheter

Background: Vascular access port (VAP) was developed for the administration of chemotherapeutic agents, minimizinglocal drug reactions and complications associated with migration of peripheral venous catheter (PVC) in humans. Thedevice is widely used in human oncology and has gained importance in veterinary oncology, especially in long treatmentregimens, as in the case of canine lymphoma. VAP favors therapy and the animals life quality. The aim of this study wasto describe the use of VAP in dogs, comparing to PVC access, during canine lymphoma chemotherapeutic treatment.Materials, Methods & Results: Eleven dogs with multicentric lymphoma which required chemotherapy were selectedfor the study. The dogs were randomly allocated to two groups with five and six animals, and each group received thechemotherapy protocol through the PVC (n= 5) or VAP (n= 6). For the sake of standardization, assessments were madewhenever the dogs received vincristine sulfate, despite the use of the infusion system in all sessions of the MadisonWisconsin protocol. A VAP was implanted into the right external jugular vein of six dogs under inhalational anesthesia,using the Seldinger technique. Systolic blood pressure (SBP) levels and handling time during chemotherapy sessions werecompared in both groups in three time periods during the procedures: 10 min after arrival to each chemotherapy (P1);immediately after placement of the PVC or puncture of the VAP reservoir (P2); and at the end of chemotherapy (P3). Thearithmetic mean of five consecutive assessments was used in each time period. In the chemotherapy sessions, the mean ofSBP variation decreased statistically significant in the VAP group compared to PVC group. SBP decreased from P1 to P2and from P1 to P3 in all sessions (S1, S2, and S3) in the VAP group, and increased in the PVC...(AU)

Occurrence of endoparasites with zoonotic potential in the city of Uruguaiana-RS / Ocorrência de endoparasitos com potencial zoonótico no município de Uruguaiana-RS

The presence of feces of companion animals in public squares contaminated with endoparasite eggs with zoonotic potential plays a fundamental role in the transmission of diseases to the population. In this context, Cutaneous Larva Migrans and Visceral Larva Migrans are important zoonosis. In the city of Uruguaiana-RS, as well as in the national panorama, there is a shortage of data that contemplate the current contamination of public areas in order to map critical areas for the implementation of public policies to prevent these diseases. Thus, we evaluated the occurrence of endoparasites in the public squares of Uruguaiana-RS through the collection of feces in these places. A total of 87 fecal samples were collected, in which 23 were positive for endoparasite eggs with zoonotic potential. In the compilation of the data, we found higher prevalence of Ancylostoma spp. than Toxocara spp. in not fenced public squares. Compared with previous studies, a decrease in contamination of feces found and collected was observed. Thus, possible causes and suggested preventive measures were raised, as well as responsible ownership and health education to reduce environmental contamination of the city.

A presença de fezes de animais de companhia em praças públicas, contaminadas com ovos de endoparasitos com potencial zoonótico, tem papel fundamental na transmissão de doenças para a população. Neste contexto, Larva Migrans Cutânea e Larva Migrans Visceral são importantes zoonoses. No município de Uruguaiana-RS, assim como no panorama nacional, há escassez de dados que contemplem a atual contaminação de áreas públicas a fim de mapear áreas críticas para a implementação de políticas públicas de prevenção destas doenças. Desta forma, se avaliou a ocorrência de endoparasitos em praças do município de Uruguaiana-RS através da coleta de fezes nestes locais. Foram coletadas 87 amostras de fezes, sendo 23 positivas para ovos de endoparasitos com potencial zoonótico. Na compilação dos dados obteve-se maior prevalência de ovos de Ancylostoma spp. que Toxocara spp. nas praças não cercadas. Em comparação com estudos anteriores percebeu-se uma diminuição da contaminação das fezes encontradas e coletadas. Sendo assim, foram levantadas possíveis causas e sugeridas medidas preventivas, além da posse responsável e educação em saúde para reduzir a contaminação ambiental do munícipio.

Occurrence of endoparasites with zoonotic potential in the city of Uruguaiana-RS / Ocorrência de endoparasitos com potencial zoonótico no município de Uruguaiana-RS

The presence of feces of companion animals in public squares contaminated with endoparasite eggs with zoonotic potential plays a fundamental role in the transmission of diseases to the population. In this context, Cutaneous Larva Migrans and Visceral Larva Migrans are important zoonosis. In the city of Uruguaiana-RS, as well as in the national panorama, there is a shortage of data that contemplate the current contamination of public areas in order to map critical areas for the implementation of public policies to prevent these diseases. Thus, we evaluated the occurrence of endoparasites in the public squares of Uruguaiana-RS through the collection of feces in these places. A total of 87 fecal samples were collected, in which 23 were positive for endoparasite eggs with zoonotic potential. In the compilation of the data, we found higher prevalence of Ancylostoma spp. than Toxocara spp. in not fenced public squares. Compared with previous studies, a decrease in contamination of feces found and collected was observed. Thus, possible causes and suggested preventive measures were raised, as well as responsible ownership and health education to reduce environmental contamination of the city.(AU)

A presença de fezes de animais de companhia em praças públicas, contaminadas com ovos de endoparasitos com potencial zoonótico, tem papel fundamental na transmissão de doenças para a população. Neste contexto, Larva Migrans Cutânea e Larva Migrans Visceral são importantes zoonoses. No município de Uruguaiana-RS, assim como no panorama nacional, há escassez de dados que contemplem a atual contaminação de áreas públicas a fim de mapear áreas críticas para a implementação de políticas públicas de prevenção destas doenças. Desta forma, se avaliou a ocorrência de endoparasitos em praças do município de Uruguaiana-RS através da coleta de fezes nestes locais. Foram coletadas 87 amostras de fezes, sendo 23 positivas para ovos de endoparasitos com potencial zoonótico. Na compilação dos dados obteve-se maior prevalência de ovos de Ancylostoma spp. que Toxocara spp. nas praças não cercadas. Em comparação com estudos anteriores percebeu-se uma diminuição da contaminação das fezes encontradas e coletadas. Sendo assim, foram levantadas possíveis causas e sugeridas medidas preventivas, além da posse responsável e educação em saúde para reduzir a contaminação ambiental do munícipio.(AU)

Otite bacteriana por Klebsiella sp. como causa de encefalite em um gato / Bacterial otitis by Klebsiella sp. as a cause of encephalitis in a cat

Background: Meningoencephalitis in cats is usually related to infectious diseases but may also be caused by the extension of bacterial infections originated in the middle or inner ear. This paper seeks to report on a case of encephalitis due to an infection in the middle/inner ear in a 15-year-old cat. The diagnosis was made through brain CT scan and culture and antibiogram of material collected during a ventral osteotomy of the tympanic bulla and reported grave intravascular haemolysis associated to the treatment with antimicrobial imipenem with cilastatin sodium that has already been described in humans but not in small animals in the researched literature.Case: A 15-year-old, male, castrated, Siamese cat was brought to the Veterinary Clinical Hospital of the UFRGS. The animal presented pyrexia and hyporexia, tested negative in the immunoenzymatic test to detect antigens of the FeLV and antibodies of the FIV. The animal had a history of three episodes of tonic convulsion within two days and otitis that had gone untreated for 10 months prior to the consultation, with apparent spontaneous remission. After blood tests, serum biochemistry profile, abdominal ultrasound scan, chest x-rays and CT scan was requested. It revealed middle and inner otitis in the right ear. It was performed a ventral osteotomy of the right tympanic bulla. Material was collected for culture and antibiogram. It was confirmed that the animal had an inner otitis of bacterial origin by multi-resistant Klebsiella spp. that was sensitive only to antimicrobial imipenem with cilastatin sodium. Fifteen days into the treatment with this antibacterial combination, grave haemolysis was observed, probably associated to the use of the medicine. The patient died on the 17th day of the treatment which was not interrupted as per its owner’s decision. At necropsy, both in the tympanic bulla and liver were found mucous content and whitish lumps.[...]

Otite bacteriana por Klebsiella sp. como causa de encefalite em um gato / Bacterial otitis by Klebsiella sp. as a cause of encephalitis in a cat

Background: Meningoencephalitis in cats is usually related to infectious diseases but may also be caused by the extension of bacterial infections originated in the middle or inner ear. This paper seeks to report on a case of encephalitis due to an infection in the middle/inner ear in a 15-year-old cat. The diagnosis was made through brain CT scan and culture and antibiogram of material collected during a ventral osteotomy of the tympanic bulla and reported grave intravascular haemolysis associated to the treatment with antimicrobial imipenem with cilastatin sodium that has already been described in humans but not in small animals in the researched literature.Case: A 15-year-old, male, castrated, Siamese cat was brought to the Veterinary Clinical Hospital of the UFRGS. The animal presented pyrexia and hyporexia, tested negative in the immunoenzymatic test to detect antigens of the FeLV and antibodies of the FIV. The animal had a history of three episodes of tonic convulsion within two days and otitis that had gone untreated for 10 months prior to the consultation, with apparent spontaneous remission. After blood tests, serum biochemistry profile, abdominal ultrasound scan, chest x-rays and CT scan was requested. It revealed middle and inner otitis in the right ear. It was performed a ventral osteotomy of the right tympanic bulla. Material was collected for culture and antibiogram. It was confirmed that the animal had an inner otitis of bacterial origin by multi-resistant Klebsiella spp. that was sensitive only to antimicrobial imipenem with cilastatin sodium. Fifteen days into the treatment with this antibacterial combination, grave haemolysis was observed, probably associated to the use of the medicine. The patient died on the 17th day of the treatment which was not interrupted as per its owners decision. At necropsy, both in the tympanic bulla and liver were found mucous content and whitish lumps.[...](AU)

Reparo de hérnia abdominal com pericárdio bovino associado a células-tronco mesenquimais em ratos Wistar / Abdominal Hernia Repair with Bovine Pericardium Seeded with Mesenchymal Stem Cells in Wistar Rats

Background: Biological membranes demonstrate superiority over synthetic ones for its biocompatibility and strength in the reduction of abdominal hernias. Recents tissue engineering researches add mesenchymal stem cells to biological membranes with the purpose of obtaining additional cellular proliferation and consequent muscle regeneration, using biological membranes as cellular scaffolds. This article aimed to study the influence of mesenchymal stem cells in muscle regeneration in abdominal hernias, reduced with biological membranes. Materials, Methods & Results: Adult Wistar rats underwent abdominal hernia-inducing. They were divided into two groups as to the form of treatment for the reduction of hernia: stem cells associated with biological membranes or only biological membranes. After the treatment the macro and microscopic reviews were carried out in days seven, 14 and 60 postoperatively. Preparation of bovine pericardium with glycerin 98% presented efficiency in decellularization and conservation, maintaining its strength and avoiding bacterial growth. The mesenchymal stem cells obtained from bone marrow of adult Wistar rats, had capacity of proliferation. The majority of the cells was positive for the expression of surface antigens CD44, CD29 and CD99 and was negative for CD 34. In the differentiation trials, the same cells were able to differentiate into adipocytes and osteocytes. With 24 h from co-cultivating adhesion of mesenchymal stem cells in the membranes was observed. There was no foreign body reaction or contamination of surgical wounds and there was intense postoperative neovascularization on seven days. All animals presented omentum adherence, but no adherence to other organs.There was no statistically difference for the different times in macroscopic assessment: deposition of fibrous tissue, implant integration. The same occurred with the microscopic evaluations between the different treatment groups. The groups of immediate and later repair presented different responses to treatment. Discussion: The use of rats as animal model was satisfactory, being suitable for surgical procedures and assessments of the abdominal cavity. The different results obtained between groups of immediate repair and late repair corroborate with the idea that there is difference between induction and repair models in the same surgery or in different surgeries with the time interval between the two, suggesting the need for methodologies that simulate the hernias chronicity. The cells used were classified as mesenchymal stem cells, because it met all the criteria of Mesenchymal and Tissue Stem Cell Committee of the International Society of Celullar Therapy. The membranes conserved with glycerin 98% demonstrated biocompatibility, because there was no rejection or necrosis, infection or exacerbated infl ammation. However the muscle regeneration was not obtained over the membranes - and the methodological difference in other latest experiments about the membranes decellularization and the co-cultivating - can leads to conclusion that the cells attached to membranes were insufficient in number to obtain the desired result. These results suggest the need of new research studies or co-cultivating times and decellularization methods of bovine pericardium for association with mesenchymal stem cells.