Achados anatomopatológicos de Toxoplasma gondii em Lagothrix lagotricha da região amazônica brasileira / Anatomopathological findings of Toxoplasma gondii in Lagothrix lagotricha from the Brazilian amazon region / Hallazgos anatomopatologicos de Toxoplasma gondii en Lagothrix lagotricha de la regiónde la amazonia brasileña

O Toxoplasma gondii é um coccídeo zoonótico e cosmopolita, com transmissão horizontal e vertical, e ciclo biológico do tipo heteroxênico, capaz de infectar uma ampla variedade de animais homeotérmicos, incluindo primatas não-humanos e humanos. A evolução desta enfermidades e manifesta de forma aguda e crônica, dependendo da relação parasito-hospedeiro, condição fisiológica do infectado e virulência, podendo levar ao óbito. Neste relato, foram realizadas necropsias de três espécimes de Lagothrix lagotricha, sendo observado edema pulmonar e aumento no tamanho dos linfonodos mesentéricos e submandibulares, assim como hepatoesplenomegalia. Amostras de diferentes tecidos foram colhidas e fixadas em formol a 10% tamponado, processadas e analisadas. Por meio da histopatologia foram identificadas estruturas do T. gondii nas formas de cistos nos tecidos de linfonodos, musculatura cardíaca, pulmões, baço e fígado e taquizoítos no tecido hepático. Assim, pode-se concluir que que este relato contribui para o conhecimento sobre a infecção por T. gondii em primatas não-humanos, considerando a possibilidade de continuidade do ciclo no ecossistema amazônico, pela observância das formas do coccídeo em diversos tecidos de L. lagotricha, sem desenvolver as alterações macroscópicas caraterísticas.

Toxoplasma gondii is a zoonotic and cosmopolitan coccid, with horizontal and vertical transmission, and a biological cycle of the heteroxenic type, capable of infecting a wide variety of homeothermic animals, including non-human primates and human. The evolution of this disease manifests itself in an acute and chronic form, depending on the parasite-host relationship, the physiological condition of the infected and virulence, which can lead to death. In this report, necropsies of three specimens of Lagothrix lagotricha was performed. In the necroscopic examination of the specimens, pulmonary edema and an increase in the size of the mesenteric and submandibular lymph nodes, as well as hepatosplenomegaly. Samples from different tissues were collected and fixed in 10% buffered formaldehyde, processed and analyzed. Through histopathology, structures of of T. gondii were identified in the forms of cystzoites in lymph node tissues, cardiac musculature, lungs, spleen and liver, and tachyzoites in liver tissue. Thus, it can be concluded that this report contributes to the knowledge about the infection by T. gondii in non-human primates, considering the possibility of continuity of the cycle in the Amazonian ecosystem, by observing the forms of the coccid in several tissues of L. lagotricha, without developing macroscopic changes characteristics.

Toxoplasma gondii es un coccidio zoonótico y cosmopolita, contransmisión horizontal y vertical, y el ciclo biológico de tipo heteroxenico, capaz de infectar una amplia variedad de animales homeotérmicos, incluidos primatas no humanos y humanos. La evolución de esta enfermedad se manifesta de forma aguda y crónica, dependiendo de la relación parásito-huésped, la condición fisiológica del infectado y la virulencia, que puede conducir a la muerte. En este informe se realizó la necropsia de tres especímenes de Lagothrix lagotricha, com edema pulmonar y aumento del tamaño de los ganglios linfáticos mesentéricos y submandibulares, así como hepatoesplenomegalia. Se recolectaron muestras de diferentes tejidos y se fijaron en formaldehído tamponado al 10%, se procesaron y analizaron. Mediante histopatología, se identificaron estructuras de T. gondii en forma de cistos en tejidos de ganglios linfáticos, musculatura cardíaca, pulmones, bazo y hígado, y taquizoítos en el tejido hepático. Así, se pudo concluir que este informe contribuye con conocimiento sobre la infección por T. gondii en primatas no humanos, considerando la posibilidad de continuidad del ciclo en el ecosistema amazónico, al observar las formas del coccidio en varios tejidos de L. lagotricha, sin desarrollar los cambios macroscópicos característicos.

Achados anatomopatológicos de Toxoplasma gondii em Lagothrix lagotricha da região amazônica brasileira / Anatomopathological findings of Toxoplasma gondii in Lagothrix lagotricha from the Brazilian amazon region / Hallazgos anatomopatologicos de Toxoplasma gondii en Lagothrix lagotricha de la regiónde la amazonia brasileña

O Toxoplasma gondii é um coccídeo zoonótico e cosmopolita, com transmissão horizontal e vertical, e ciclo biológico do tipo heteroxênico, capaz de infectar uma ampla variedade de animais homeotérmicos, incluindo primatas não-humanos e humanos. A evolução desta enfermidades e manifesta de forma aguda e crônica, dependendo da relação parasito-hospedeiro, condição fisiológica do infectado e virulência, podendo levar ao óbito. Neste relato, foram realizadas necropsias de três espécimes de Lagothrix lagotricha, sendo observado edema pulmonar e aumento no tamanho dos linfonodos mesentéricos e submandibulares, assim como hepatoesplenomegalia. Amostras de diferentes tecidos foram colhidas e fixadas em formol a 10% tamponado, processadas e analisadas. Por meio da histopatologia foram identificadas estruturas do T. gondii nas formas de cistos nos tecidos de linfonodos, musculatura cardíaca, pulmões, baço e fígado e taquizoítos no tecido hepático. Assim, pode-se concluir que que este relato contribui para o conhecimento sobre a infecção por T. gondii em primatas não-humanos, considerando a possibilidade de continuidade do ciclo no ecossistema amazônico, pela observância das formas do coccídeo em diversos tecidos de L. lagotricha, sem desenvolver as alterações macroscópicas caraterísticas.(AU)

Toxoplasma gondii is a zoonotic and cosmopolitan coccid, with horizontal and vertical transmission, and a biological cycle of the heteroxenic type, capable of infecting a wide variety of homeothermic animals, including non-human primates and human. The evolution of this disease manifests itself in an acute and chronic form, depending on the parasite-host relationship, the physiological condition of the infected and virulence, which can lead to death. In this report, necropsies of three specimens of Lagothrix lagotricha was performed. In the necroscopic examination of the specimens, pulmonary edema and an increase in the size of the mesenteric and submandibular lymph nodes, as well as hepatosplenomegaly. Samples from different tissues were collected and fixed in 10% buffered formaldehyde, processed and analyzed. Through histopathology, structures of of T. gondii were identified in the forms of cystzoites in lymph node tissues, cardiac musculature, lungs, spleen and liver, and tachyzoites in liver tissue. Thus, it can be concluded that this report contributes to the knowledge about the infection by T. gondii in non-human primates, considering the possibility of continuity of the cycle in the Amazonian ecosystem, by observing the forms of the coccid in several tissues of L. lagotricha, without developing macroscopic changes characteristics.(AU)

Toxoplasma gondii es un coccidio zoonótico y cosmopolita, contransmisión horizontal y vertical, y el ciclo biológico de tipo heteroxenico, capaz de infectar una amplia variedad de animales homeotérmicos, incluidos primatas no humanos y humanos. La evolución de esta enfermedad se manifesta de forma aguda y crónica, dependiendo de la relación parásito-huésped, la condición fisiológica del infectado y la virulencia, que puede conducir a la muerte. En este informe se realizó la necropsia de tres especímenes de Lagothrix lagotricha, com edema pulmonar y aumento del tamaño de los ganglios linfáticos mesentéricos y submandibulares, así como hepatoesplenomegalia. Se recolectaron muestras de diferentes tejidos y se fijaron en formaldehído tamponado al 10%, se procesaron y analizaron. Mediante histopatología, se identificaron estructuras de T. gondii en forma de cistos en tejidos de ganglios linfáticos, musculatura cardíaca, pulmones, bazo y hígado, y taquizoítos en el tejido hepático. Así, se pudo concluir que este informe contribuye con conocimiento sobre la infección por T. gondii en primatas no humanos, considerando la posibilidad de continuidad del ciclo en el ecosistema amazónico, al observar las formas del coccidio en varios tejidos de L. lagotricha, sin desarrollar los cambios macroscópicos característicos.(AU)

Uterine adenomyosis in bitches and queens: occurrence and histopathological aspects / Adenomiose uterina em cadelas e gatas: ocorrência e aspectos histopatológicos

The goal of this study was to report the occurrence and histopathological aspects of uterine adenomyosis in bitches and queens. The sample consisted of 200 uteri from bitches (n =100) and queens (n = 100) obtained from elective ovarian-salpingohysterectomies. The animals were classified according to age, having given birth, and contraceptive use. Samples of the uterine horns were collected following routine histological processing using slides stained with hematoxylin and eosin. The data obtained were analysed using the chi-square test, with 5% significance level. Uterine adenomyosis was confirmed in 12% of bitches and 3% of queens. Age had no influence on the occurrence of this disease; however, not having given birth and the use of contraceptives favoured its appearance in bitches. Microscopically, portions of endometrial glands and stroma were present in the circular and vascular myometrial strata, with normal and cystic characteristics and variations in the glandular epithelium. There was cystic-type luminal content, such as calcification, hyaline substance, polyps, and mucin. This study made it possible to conclude that uterine adenomyosis occurs more frequently in bitches than in queens, regardless of age groups. Nulliparous bitches using contraceptives were the most affected. This pathology showed variations with respect to location and histopathological aspects, with the cystic type exhibiting calcification, hyaline substance, polyps, and mucin inside the lumen. These findings are being reported for the first time in the veterinary literature.

Objetivou-se com essa pesquisa relatar a ocorrência e os aspectos histopatológicos da adenomiose uterina em cadelas e gatas. Foram utilizados 200 úteros, de cadelas (n=100) e gatas (n=100), procedentes de ovariosalpingohisterectomias eletivas. Os animais foram classificados segundo a faixa etária, parição e uso de contraceptivos. Amostras dos cornos uterinos foram coletadas, seguindo-se ao processamento histológico de rotina, sendo as lâminas coradas pela hematoxilina e eosina. Os dados obtidos foram analisados pelo teste Qui-quadrado, com significância de 5%. A adenomiose uterina foi identificada em 12% das cadelas e 3% das gatas. Não houve influência na ocorrência dessa enfermidade quanto a faixa etária, porém a ausência de parição e o uso de contraceptivos favoreceram o aparecimento em cadelas. Microscopicamente, porções de glândulas e estroma endometrial estavam presentes no estrato miometrial circular e vascular, ocorrendo o tipo normal, cístico e variações no epitélio glandular. Houve presença de conteúdo luminal no tipo cístico, como calcificação, substância hialina, pólipos e mucina. Com esse estudo foi possível concluir que a adenomiose uterina ocorre com maior frequência em cadelas do que em gatas, independente da faixa etária. Cadelas nulíparas que utilizaram contraceptivos foram as mais afetadas. Essa patologia mostrou variações quanto a localização e aspecto histopatológico, exibindo no tipo cístico, calcificação, substância hialina, pólipos e mucina no interior do lúmen, sendo estes achados relatados pela primeira vez na literatura veterinária.

Uterine adenomyosis in bitches and queens: occurrence and histopathological aspects / Adenomiose uterina em cadelas e gatas: ocorrência e aspectos histopatológicos

The goal of this study was to report the occurrence and histopathological aspects of uterine adenomyosis in bitches and queens. The sample consisted of 200 uteri from bitches (n =100) and queens (n = 100) obtained from elective ovarian-salpingohysterectomies. The animals were classified according to age, having given birth, and contraceptive use. Samples of the uterine horns were collected following routine histological processing using slides stained with hematoxylin and eosin. The data obtained were analysed using the chi-square test, with 5% significance level. Uterine adenomyosis was confirmed in 12% of bitches and 3% of queens. Age had no influence on the occurrence of this disease; however, not having given birth and the use of contraceptives favoured its appearance in bitches. Microscopically, portions of endometrial glands and stroma were present in the circular and vascular myometrial strata, with normal and cystic characteristics and variations in the glandular epithelium. There was cystic-type luminal content, such as calcification, hyaline substance, polyps, and mucin. This study made it possible to conclude that uterine adenomyosis occurs more frequently in bitches than in queens, regardless of age groups. Nulliparous bitches using contraceptives were the most affected. This pathology showed variations with respect to location and histopathological aspects, with the cystic type exhibiting calcification, hyaline substance, polyps, and mucin inside the lumen. These findings are being reported for the first time in the veterinary literature.(AU)

Objetivou-se com essa pesquisa relatar a ocorrência e os aspectos histopatológicos da adenomiose uterina em cadelas e gatas. Foram utilizados 200 úteros, de cadelas (n=100) e gatas (n=100), procedentes de ovariosalpingohisterectomias eletivas. Os animais foram classificados segundo a faixa etária, parição e uso de contraceptivos. Amostras dos cornos uterinos foram coletadas, seguindo-se ao processamento histológico de rotina, sendo as lâminas coradas pela hematoxilina e eosina. Os dados obtidos foram analisados pelo teste Qui-quadrado, com significância de 5%. A adenomiose uterina foi identificada em 12% das cadelas e 3% das gatas. Não houve influência na ocorrência dessa enfermidade quanto a faixa etária, porém a ausência de parição e o uso de contraceptivos favoreceram o aparecimento em cadelas. Microscopicamente, porções de glândulas e estroma endometrial estavam presentes no estrato miometrial circular e vascular, ocorrendo o tipo normal, cístico e variações no epitélio glandular. Houve presença de conteúdo luminal no tipo cístico, como calcificação, substância hialina, pólipos e mucina. Com esse estudo foi possível concluir que a adenomiose uterina ocorre com maior frequência em cadelas do que em gatas, independente da faixa etária. Cadelas nulíparas que utilizaram contraceptivos foram as mais afetadas. Essa patologia mostrou variações quanto a localização e aspecto histopatológico, exibindo no tipo cístico, calcificação, substância hialina, pólipos e mucina no interior do lúmen, sendo estes achados relatados pela primeira vez na literatura veterinária.(AU)

Paraganglioma de corpo aórtico em cão / Aortic body paraganglioma in dog

Background: Aortic body paragangliomas are uncommon neoplasms that develop mainly in aortic and carotid bodies. It has been supposed that genetic factors and chronic hypoxia may stimulate tumor development. The brachycephalic dog breeds, as Boxer, are most predisposed to present this neoplasm. The clinical symptomatology is related to tumor size and localization. Usually aortic body paraganglioma has benign biological behavior, when it is malignant, rarely promotes metastases. The aim of this study was to report a case of the aortic body paraganglioma as death cause in a dog. Case: A canine, 10-year-old, male, cross breed, presented clinical signs as anorexia, emesis, cough, dyspnea and exercise intolerance. After death the animal was examined at the Department of Veterinary Pathology at the Federal Rural University of Amazonia. On necropsy, no pericardial effusion was identified, however pleural and abdominal effusion was observed, volume like 1000 and 700 mL, respectively. The heart had a neoplasm near the left atrium, it measured 6.5 x 8.2 cm, had irregular surface, firm consistency, grayish color, and at the cut showed infiltration in the myocardium, as well as obstruction of the left atrial lumen and left ventricle concentric hypertrophy. No distant metastases were found. Microscopically, the tumor consisted of polyhedral morphology cells, eosinophilic cytoplasm, spherical and hyperchromatic nucleus. Cells were grouped into lobes separated by fibrovascular stroma, large cells (less uniform cells), low mitotic rate and myocardial infiltration. On immunohistochemical analysis anti-cytokeratin, anti-vimentin and anti-S-100 antibodies were used. Tumour cells stained was absent for anti-cytokeratin and anti-vimentin, but was anti-S-100 positive. A case of malignant aortic body paraganglioma grade II was diagnosed.[…](AU)

Paraganglioma de corpo aórtico em cão / Aortic body paraganglioma in dog

Background: Aortic body paragangliomas are uncommon neoplasms that develop mainly in aortic and carotid bodies. It has been supposed that genetic factors and chronic hypoxia may stimulate tumor development. The brachycephalic dog breeds, as Boxer, are most predisposed to present this neoplasm. The clinical symptomatology is related to tumor size and localization. Usually aortic body paraganglioma has benign biological behavior, when it is malignant, rarely promotes metastases. The aim of this study was to report a case of the aortic body paraganglioma as death cause in a dog. Case: A canine, 10-year-old, male, cross breed, presented clinical signs as anorexia, emesis, cough, dyspnea and exercise intolerance. After death the animal was examined at the Department of Veterinary Pathology at the Federal Rural University of Amazonia. On necropsy, no pericardial effusion was identified, however pleural and abdominal effusion was observed, volume like 1000 and 700 mL, respectively. The heart had a neoplasm near the left atrium, it measured 6.5 x 8.2 cm, had irregular surface, firm consistency, grayish color, and at the cut showed infiltration in the myocardium, as well as obstruction of the left atrial lumen and left ventricle concentric hypertrophy. No distant metastases were found. Microscopically, the tumor consisted of polyhedral morphology cells, eosinophilic cytoplasm, spherical and hyperchromatic nucleus. Cells were grouped into lobes separated by fibrovascular stroma, large cells (less uniform cells), low mitotic rate and myocardial infiltration. On immunohistochemical analysis anti-cytokeratin, anti-vimentin and anti-S-100 antibodies were used. Tumour cells stained was absent for anti-cytokeratin and anti-vimentin, but was anti-S-100 positive. A case of malignant aortic body paraganglioma grade II was diagnosed.[…]

Schistosomus reflexus in a dog and a cat

Background: Schistosomus reflexus (SR) is a congenital anomaly, that the animal presents exposed abdominal and thoracic organs. The alterations includes limbs malformation, abnormal spinal column, liver and diaphragm hypoplasia, genitourinary and gastrointestinal changes, on this way, the fetus can not survive. The aim of this study was to report the first SR case in dog and cat in Brazil, and describe anatomical, pathological and radiographic changes. Case: The puppy had ventral midline defect in the abdominal and thoracic wall extending from the sternum to the pubis with viscera exposition, including the heart, lung, liver, stomach, intestine, spleen, kidneys, moreover right army was abnormal, however no spinal column was present. It was performed radiography to evaluate bone abnormalities, and was diagnosed agenesis of carpal, metacarpal and phalange bones. The kitten had ventral midline defect measuring 4 x 2 cm, in the abdominal wall and exposition of liver, stomach, intestine and spleen, as well as tibiotarsal joint arthrogryposis on both lags. It was performed fetus radiography and no spinal column change was diagnosed. Discussion: Schistosomus reflexus is a congenital abnormality common in ruminants. Although it was few reported in dogs and cats. The two cases in this study are the firsts reposts in Brazil in these especies. In our case, the dog had exposition of abdominal and thoracic organs and died one hour after born, similar to this, another author reported a case where the thoracic and abdominal evisceration and the puppy died ten minutes after born. However, in another case with abdominal evisceration only, the puppy survived for eight days. Probably it happened because respiratore function was not compromised. The kitten had abdominal opening only, in turn, other authors reported a case whose abdominal viscera exposition was by pelvic fissure, and another case, what presented thoracic and abdominal evisceration, like our dog case...

Schistosomus reflexus in a dog and a cat

Background: Schistosomus reflexus (SR) is a congenital anomaly, that the animal presents exposed abdominal and thoracic organs. The alterations includes limbs malformation, abnormal spinal column, liver and diaphragm hypoplasia, genitourinary and gastrointestinal changes, on this way, the fetus can not survive. The aim of this study was to report the first SR case in dog and cat in Brazil, and describe anatomical, pathological and radiographic changes. Case: The puppy had ventral midline defect in the abdominal and thoracic wall extending from the sternum to the pubis with viscera exposition, including the heart, lung, liver, stomach, intestine, spleen, kidneys, moreover right army was abnormal, however no spinal column was present. It was performed radiography to evaluate bone abnormalities, and was diagnosed agenesis of carpal, metacarpal and phalange bones. The kitten had ventral midline defect measuring 4 x 2 cm, in the abdominal wall and exposition of liver, stomach, intestine and spleen, as well as tibiotarsal joint arthrogryposis on both lags. It was performed fetus radiography and no spinal column change was diagnosed. Discussion: Schistosomus reflexus is a congenital abnormality common in ruminants. Although it was few reported in dogs and cats. The two cases in this study are the firsts reposts in Brazil in these especies. In our case, the dog had exposition of abdominal and thoracic organs and died one hour after born, similar to this, another author reported a case where the thoracic and abdominal evisceration and the puppy died ten minutes after born. However, in another case with abdominal evisceration only, the puppy survived for eight days. Probably it happened because respiratore function was not compromised. The kitten had abdominal opening only, in turn, other authors reported a case whose abdominal viscera exposition was by pelvic fissure, and another case, what presented thoracic and abdominal evisceration, like our dog case...(AU)

Hepatic capillariasis in two captive Callithrix penicillata / Capilariose hepática em dois Callithrix penicillata de cativeiro

This study aimed to describe the occurrence of hepatic capillariasis in two non-human primates of the species Callithrix penicillata that were kept in captivity. Case 1 was in a young female, whose liver presented pale coloring, softening due to autolysis and several noticeably whitish areas. Case 2 was in a male of unknown age, whose liver was slightly pale and brownish. Histological analysis on the samples revealed extensive interstitial fibrosis with hemosiderotic areas. Adult nematodes of Capillaria hepatica and numerous eggs, morphologically characterized by their non-embryonic ellipsoid shape, with bipolar plugs that did not protrude from the shell, thick and birefringent, with a porous layer, thus giving a striated appearance to the eggs. Some cystic formations presented nematode eggs surrounded by numerous foreign-body giant cells. The literature consulted made little reference to reports of Capillaria hepatica in non-human primates. However, similar infections have been extensively studied in domestic rodents.(AU)

O presente trabalho relata a ocorrência de dois casos de capilariose hepática em primatas não-humanos da espécie Callithrix penicilatta mantidos em cativeiro. O caso 1 era uma fêmea jovem, que exibiu fígado de cor pálida, suavizada devido a autólise e algumas áreas múltiplas visivelmente esbranquiçadas. O caso 2 foi registrado em um macho adulto, de idade desconhecida, com fígado levemente pálido e acastanhado. A análise histológica das amostras revelou extensa fibrose intersticial com áreas de hemossiderose, exemplares de Capillaria hepatica adultos e numerosos ovos morfologicamente caracterizados pela sua forma elipsóide, não-embrionárias, com tampões bipolares que se sobressaem da casca, que é espessa, birrefringente e com uma camada porosa, dando uma aparência estriada ao ovo. Algumas formações císticas continham ovos do nematóide cercados por numerosas células gigantes do tipo corpo estranho. A literatura consultada possui poucas referências acerca da presença de Capillaria hepatica em primatas não-humanos. No entanto, infecções semelhantes são bem estudadas em roedores domésticos.(AU)

Hepatic capillariasis in two captive Callithrix penicillata / Capilariose hepática em dois Callithrix penicillata de cativeiro

This study aimed to describe the occurrence of hepatic capillariasis in two non-human primates of the species Callithrix penicillata that were kept in captivity. Case 1 was in a young female, whose liver presented pale coloring, softening due to autolysis and several noticeably whitish areas. Case 2 was in a male of unknown age, whose liver was slightly pale and brownish. Histological analysis on the samples revealed extensive interstitial fibrosis with hemosiderotic areas. Adult nematodes of Capillaria hepatica and numerous eggs, morphologically characterized by their non-embryonic ellipsoid shape, with bipolar plugs that did not protrude from the shell, thick and birefringent, with a porous layer, thus giving a striated appearance to the eggs. Some cystic formations presented nematode eggs surrounded by numerous foreign-body giant cells. The literature consulted made little reference to reports of Capillaria hepatica in non-human primates. However, similar infections have been extensively studied in domestic rodents.(AU)

O presente trabalho relata a ocorrência de dois casos de capilariose hepática em primatas não-humanos da espécie Callithrix penicilatta mantidos em cativeiro. O caso 1 era uma fêmea jovem, que exibiu fígado de cor pálida, suavizada devido a autólise e algumas áreas múltiplas visivelmente esbranquiçadas. O caso 2 foi registrado em um macho adulto, de idade desconhecida, com fígado levemente pálido e acastanhado. A análise histológica das amostras revelou extensa fibrose intersticial com áreas de hemossiderose, exemplares de Capillaria hepatica adultos e numerosos ovos morfologicamente caracterizados pela sua forma elipsóide, não-embrionárias, com tampões bipolares que se sobressaem da casca, que é espessa, birrefringente e com uma camada porosa, dando uma aparência estriada ao ovo. Algumas formações císticas continham ovos do nematóide cercados por numerosas células gigantes do tipo corpo estranho. A literatura consultada possui poucas referências acerca da presença de Capillaria hepatica em primatas não-humanos. No entanto, infecções semelhantes são bem estudadas em roedores domésticos.(AU)

Hepatic capillariasis in two captive Callithrix penicillata / Capilariose hepática em dois Callithrix penicillata de cativeiro

This study aimed to describe the occurrence of hepatic capillariasis in two non-human primates of the species Callithrix penicillata that were kept in captivity. Case 1 was in a young female, whose liver presented pale coloring, softening due to autolysis and several noticeably whitish areas. Case 2 was in a male of unknown age, whose liver was slightly pale and brownish. Histological analysis on the samples revealed extensive interstitial fibrosis with hemosiderotic areas. Adult nematodes of Capillaria hepatica and numerous eggs, morphologically characterized by their non-embryonic ellipsoid shape, with bipolar plugs that did not protrude from the shell, thick and birefringent, with a porous layer, thus giving a striated appearance to the eggs. Some cystic formations presented nematode eggs surrounded by numerous foreign-body giant cells. The literature consulted made little reference to reports of Capillaria hepatica in non-human primates. However, similar infections have been extensively studied in domestic rodents.

O presente trabalho relata a ocorrência de dois casos de capilariose hepática em primatas não-humanos da espécie Callithrix penicilatta mantidos em cativeiro. O caso 1 era uma fêmea jovem, que exibiu fígado de cor pálida, suavizada devido a autólise e algumas áreas múltiplas visivelmente esbranquiçadas. O caso 2 foi registrado em um macho adulto, de idade desconhecida, com fígado levemente pálido e acastanhado. A análise histológica das amostras revelou extensa fibrose intersticial com áreas de hemossiderose, exemplares de Capillaria hepatica adultos e numerosos ovos morfologicamente caracterizados pela sua forma elipsóide, não-embrionárias, com tampões bipolares que se sobressaem da casca, que é espessa, birrefringente e com uma camada porosa, dando uma aparência estriada ao ovo. Algumas formações císticas continham ovos do nematóide cercados por numerosas células gigantes do tipo corpo estranho. A literatura consultada possui poucas referências acerca da presença de Capillaria hepatica em primatas não-humanos. No entanto, infecções semelhantes são bem estudadas em roedores domésticos.

Squamous cell carcinoma in third eyelid of cat / Carcinoma de células escamosas em terceira pálpebra de felino

Background: The third eyelid neoplasms are uncommon in cats. The squamous cell carcinoma are easily found in head and neck of same species, although is unusual in eye region. The more common localization is eyelid and eyeball, being 60 and 15%, respectively. It could be diagnosed by citology, histopathology, imunohistochemistry and molecular biology. The surgery is more effective treatment, because the tumor can be totally removed and it must available surgical margin. The aim of this study was to report a case of squamous cell carcinoma in third eyelid of a cat and show how it was treated with radical surgery.Case: A 11-year-old spayed female domestic short-haired white and black colored cat was presented for evaluation at Maria Dias Teixeira Hospital of Amazonia Federal Rural University (UFRA), of an red ocular mass fast growth in the left eye for 2 months. Physical exam was within normal limits. The animal presented discomfort on the region, when it was manipulated. The mass was ulcerated and blood-tinged ocular discharge, had 3.3 x 2 cm, beginning on third eyelid and overlay all the eyeball. Blood was collated to make exams. Complete blood count and serum chemistry profiles were within normal ranges, but leukocytes were increased and it was treated with Amoxicillin (22 mg/kg). It was performed biopsy to histopathology and immunohistochemistry diagnose, and radiography and [...]

Adenocarcinoma ovariano em cadela / Ovarian adenocarcinoma in a bitch

An ovarian adenocarcinoma in a 16-years-old female Poodle is described. The material was sent to theVeterinary Pathology Laboratory at UFRA to macroscopic and microscopic examination using using the routineprocess technique with haematoxylin and eosin. Grossly, in the ovary multiple cysts filled with serous fluid wereobserved in both ovaries. Histologically, papillary growth and neoplastic tissue was noticed. According to thealterations observed, the diagnostic of ovarian adenocarcinoma was confirmed.(AU)

Squamous cell carcinoma in third eyelid of cat / Carcinoma de células escamosas em terceira pálpebra de felino

Background: The third eyelid neoplasms are uncommon in cats. The squamous cell carcinoma are easily found in head and neck of same species, although is unusual in eye region. The more common localization is eyelid and eyeball, being 60 and 15%, respectively. It could be diagnosed by citology, histopathology, imunohistochemistry and molecular biology. The surgery is more effective treatment, because the tumor can be totally removed and it must available surgical margin. The aim of this study was to report a case of squamous cell carcinoma in third eyelid of a cat and show how it was treated with radical surgery.Case: A 11-year-old spayed female domestic short-haired white and black colored cat was presented for evaluation at Maria Dias Teixeira Hospital of Amazonia Federal Rural University (UFRA), of an red ocular mass fast growth in the left eye for 2 months. Physical exam was within normal limits. The animal presented discomfort on the region, when it was manipulated. The mass was ulcerated and blood-tinged ocular discharge, had 3.3 x 2 cm, beginning on third eyelid and overlay all the eyeball. Blood was collated to make exams. Complete blood count and serum chemistry profiles were within normal ranges, but leukocytes were increased and it was treated with Amoxicillin (22 mg/kg). It was performed biopsy to histopathology and immunohistochemistry diagnose, and radiography and [...](AU)

Adenocarcinoma ovariano em cadela / Ovarian adenocarcinoma in a bitch

An ovarian adenocarcinoma in a 16-years-old female Poodle is described. The material was sent to theVeterinary Pathology Laboratory at UFRA to macroscopic and microscopic examination using using the routineprocess technique with haematoxylin and eosin. Grossly, in the ovary multiple cysts filled with serous fluid wereobserved in both ovaries. Histologically, papillary growth and neoplastic tissue was noticed. According to thealterations observed, the diagnostic of ovarian adenocarcinoma was confirmed.

Ocorrência e aspectos macro e microscópicos da mucometra em cadelas / Occurrence and macro and microscopic aspects of mucometra in bitches / Ocurrencia y los aspectos macro y microscópicos de mucometra en perras

A mucometra é uma alteração não inflamatória que afeta o útero por meio do acúmulo de fluído estéril no lúmen uterino. O presente trabalho objetivou relatar a ocorrência de mucometra em cadelas, bem como descrever seus aspectos macroscópicos e microscópicos. Foram utilizados 100 úteros de cadelas procedentes de ovário-histerectomia (OSH) de conveniência. As peças foram fixadas inteiras em formaldeído a 10% tamponado por 48 horas. Posteriomente, amostras de 3 a 5 mm de espessura foram selecionadas dos cornos uterinos, seguindo-se ao processamento histológico de rotina, com os cortes corados com Hematoxilina e Eosina. Os animais deste estudo foram classificados segundo a faixa etária, o número de partos e o uso de contraceptivos. Os dados obtidos foram tabulados e analisados estatisticamente por meio de análise descritiva e teste Qui-quadrado, com nível de significância de 5%. A mucometra foi observada em 9% (9/100) dos úteros analisados. As cadelas acima de seis meses e menor ou igual a três anos (66,67%), nulíparas (88,89%) e que fizeram uso de contraceptivos (66,67%) foram mais acometidas por mucometra. Macroscopicamente, os úteros mostraram variável distensão dos cornos, conferindo irregularidade ao órgão. Ao corte observou-se a presença de fluido mucoso e de coloração clara ou acinzentada. Ao exame histopatológico, o perimétrio e o miométrio estavam normais. Em alguns casos o endométrio estava atrofiado e, em todos os casos, as glândulas endometriais estavam distendidas e muitas delas eram císticas, apresentando ou não hiperplasia do epitélio. No lúmen glandular foi observado material homogêneo basofílico e células descamadas. Conclui-se que a mucometra é uma alteração patológica com baixa incidência em cadelas, que causa irregularidade na superfície uterina e destruição do endométrio.

The mucometra is a non-inflammatory change that affects the uterus through the accumulation of sterile fluid in the uterine lumen. This study aimed to report the occurrence of mucometra in bitches, as well as describe their macroscopic and microscopic aspects. Were used 100 uterus of bitches coming from ovariohysterectomy (OHE) of convenience. The pieces were fixed whole in 10% buffered formaldehyde for 48 hours. Subsequently, samples 3-5 mm thickness of the uterine horns were selected, followed by routine histology, with slides stained with hematoxylin and eosin. The animals in this study were classified according to age, the number of births and the use of contraceptives. Data were statistically analyzed using descriptive and chi-square analysis with level of significance of 5%. The mucometra was observed in 9% (9/100) of uteri examined. Bitches over six months and less than or equal to three years (66.67%), nulliparous (88.89%) and who used contraceptives (66.67%) were the most affected by mucometra. Grossly, the uterus showed variable distension of the horns, giving irregularity to the organ. When cut, it was observed the presence of mucous or fluid and light gray color. Histopathology, the perimetrium and myometrium were normal. In some cases, the endometrium was atrophied and in all cases the endometrial glands were distended and many cystic, either with or without hyperplasia of the epithelium. In the glandular lumen was observed homogeneous material and basophilic squamous cells. We concluded that the mucometra is a pathological change with low incidence in female dogs, which causes irregularity in the uterine lining and destruction of the endometrial mucosa.

La mucometra es un cambio no inflamatoria que afecta el útero a través de la acumulación de fluido estéril en el lumen uterino. Este estudio tuvo como objetivo informar sobre la existencia de mucometra en las perras, así como la descripción de sus aspectos macroscópicos y microscópicos. Se utilizaron 100 úteros de hembras procedentes de ovariohisterectomía (OHT) de conveniencia. Las piezas fueron fijadas toda en formaldehído tamponado en 10% durante 48 horas. Posteriomente, las muestras de 3-5 mm de espesor fueron seleccionados de los cuernos uterinos, seguido por el procesamiento histológico de rutina, con las secciones teñidas con hematoxilina y eosina. Los animales de este estudio fueron clasificados de acuerdo a la edad, el número de nacimientos y el uso de anticonceptivos. Los datos fueron analizados estadísticamente mediante análisis descriptivo y la prueba de X-cuadrado en nivel de significación del 5%. La mucometra se observó en 9% (9/ 100) de los úteros examinados. Perras con más de seis meses y menores o iguales a tres años (66,67 %), nulíparas (88,89%) y las que usaban anticonceptivos (66,67 %) fueron las más afectadas por mucometra. Macroscópicamente, los úteros mostraron distensión variable de los cuernos, dando irregularidad al órgano. Al corte, se observó la presencia de color gris y mucosidad fluida. Histopatología, el perimetrio y miometrio fueron normales. En algunos casos, el endometrio se atrofia y en todos los casos las glándulas endometriales eran distendidas y muchas eran quísticas, ya sea con o sin hiperplasia del epitelio. En el lumen glandular se observó material homogéneo basofílico y células escamosas. Llegamos a la conclusión que la mucometra es un cambio patológico con baja incidencia en perras, lo que provoca irregularidad en el revestimiento del útero y destrucción de la mucosa endometrial.

Ocorrência de parasitas de importância zoonótica: Ancylostoma spp. e Toxocara spp., em cães da região metropolitana de Belém, Pará / Occurrence of parasites of zoonotic importance: Ancylostoma spp. and Toxocara spp. in dogs in metropolitanregion of Belém, Pará, Brazil

Com a crescente aquisição de cães pela população e o grande número de cães errantes em locaispúblicos, objetivou-se com este estudo realizar o diagnóstico de helmintos intestinais zoonóticos causadores delarva migrans cutânea por Ancylostoma spp. e visceral por Toxocara spp. em animais da região Metropolitana deBelém, Pará, Brasil. Para tanto, foram coletadas 150 amostras de fezes de cães (101 domiciliados e 49 errantes),que foram analisadas utilizando a técnica de Hoffman. Do total de amostras analisadas, 21% foram positivaspara ovos de helmintos. Dos cães afetados 0,6% eram domiciliados e 51% errantes. Ancylostoma spp. foi ohelminto mais frequente, ocorrendo em animais de ambas as faixas etárias e sexos. Esses resultados mostram queanimais errantes são mais susceptíveis a infecção por parasitas e revelam uma problemática de saúde públicapela possibilidade de transmissão desses parasitas aos humanos

In view of the growing ownership of dogs by people and the large number of stray dogs in publicplaces, the objective of this study was to determine the zoonotic intestinal helminths that cause cutaneous larvamigrans by Ancylostoma spp. and visceral larva migrans by Toxocara spp. in dogs in the metropolitan region ofBelém, PA, Brazil. Therefore, we collected 150 samples of feces of dogs (101 domestic animals and 49 straydogs), were analyzed by the Hoffman method. Of the total samples analyzed, 21% were positive for helmintheggs. Of the dogs affected 0.6% were house dogs and 51% street dogs. Ancylostoma spp. were the most commonhelminths, occurring in animals of both age and gender groups studied. These results show that stray animals aremore susceptible to infection by parasites and reveal a public health problem by the possibility of transmission ofparasites to humans

Ocorrência de parasitas de importância zoonótica: Ancylostoma spp. e Toxocara spp., em cães da região metropolitana de Belém, Pará / Occurrence of parasites of zoonotic importance: Ancylostoma spp. and Toxocara spp. in dogs in metropolitanregion of Belém, Pará, Brazil

Com a crescente aquisição de cães pela população e o grande número de cães errantes em locaispúblicos, objetivou-se com este estudo realizar o diagnóstico de helmintos intestinais zoonóticos causadores delarva migrans cutânea por Ancylostoma spp. e visceral por Toxocara spp. em animais da região Metropolitana deBelém, Pará, Brasil. Para tanto, foram coletadas 150 amostras de fezes de cães (101 domiciliados e 49 errantes),que foram analisadas utilizando a técnica de Hoffman. Do total de amostras analisadas, 21% foram positivaspara ovos de helmintos. Dos cães afetados 0,6% eram domiciliados e 51% errantes. Ancylostoma spp. foi ohelminto mais frequente, ocorrendo em animais de ambas as faixas etárias e sexos. Esses resultados mostram queanimais errantes são mais susceptíveis a infecção por parasitas e revelam uma problemática de saúde públicapela possibilidade de transmissão desses parasitas aos humanos(AU)

In view of the growing ownership of dogs by people and the large number of stray dogs in publicplaces, the objective of this study was to determine the zoonotic intestinal helminths that cause cutaneous larvamigrans by Ancylostoma spp. and visceral larva migrans by Toxocara spp. in dogs in the metropolitan region ofBelém, PA, Brazil. Therefore, we collected 150 samples of feces of dogs (101 domestic animals and 49 straydogs), were analyzed by the Hoffman method. Of the total samples analyzed, 21% were positive for helmintheggs. Of the dogs affected 0.6% were house dogs and 51% street dogs. Ancylostoma spp. were the most commonhelminths, occurring in animals of both age and gender groups studied. These results show that stray animals aremore susceptible to infection by parasites and reveal a public health problem by the possibility of transmission ofparasites to humans(AU)

Ectopia cordis torácica em canino: relato de caso / Thoracic ectopia cordis in canine - case report / Ectopia cordis torácica en canino: reporte de caso

A ectopia cordis é uma condição patológica caracterizada pelo posicionamento do coração fora da cavidade torácica, que acomete algumas espécies, sendo rara em canídeos. O presente relato descreve a ocorrência de um caso de ectopia cordis em um canino natimorto. Ao exame necroscópico observou-se o desvio do coração, por meio de uma fissura mediana esternal, em localização peitoral associada a alterações no pericárdio, concluindo o diagnóstico de ectopia cordis tóracica.

The ectopia cordis is a pathological condition characterized by the positioning of the heart outside the thoracic cavity, which affects some species, being rare in dogs. This report describes the occurrence of a case of ectopia cordis in a stillbirth canine. At autopsy could be observed the deviation of the heart through a medium sternal cleft, in pectoral location associated with alterations in the pericardium, concluding the diagnosis of thoracic ectopia cordis.

La ectopia cordis és una condición patológica caracterizada por el posicionamiento del corazón fuera de la cavidad torácica que afecta algunas especies, no obstante es rara em cánidos. El presente informe describe la aparición de un caso de ectopia cordis en um mortinato canino. En examen se observó la desviación del corazón por medio de una grieta mediana esternal en ubicación pectoral asociada a cambios en el pericárdio concluyendo el diagnóstico de ectopia cordis torácica.

Compressive myelopathy in a free-ranging red brocket (Mazama americana) caused by a lumbar paraspinal abscess with accompanying spondylodiscitis

Background: Spondylodiscitis is an inflammation that is characterized by the involvement of one and more intervertebral discs and adjacent vertebral bodies. The clinical manifestations of spondylodiscitis are nonspecific and vary with the site and extent of injury, however this kind of lesion is poorly known between free-ranging cervids. In this sense, the presente study aimed to describe the occurrence, clinical, radiographic and anatomopathological findings related to a lumbar abscess accompanied by spondylodiscitis in a free-ranging female red brocket (Mazama americana) from Eastern Brazilian Amazon. Case: An approximately five-month old, 3,7 kg, female red brocket (Mazama americana), was received at the Veterinary Hospital of the Federal Rural University of Amazônia, in the municipality of Belém, in the State of Pará (Northern Brazil) for clinical care. The animal was rescued from the wild by the Brazilian Institute of Environment and Renewable Natural and presented lethargy, pelvic limb paralysis with proprioceptive deficits. The clinical signs observed suggested a spinal cord injury in the thoracolumbar region and the animal was sent to radiographic examination and myelography performed on the ventrodorsal and lateral projections of the vertebrae and spinal cord. The hemogram revealed anemia (8.74 × 106 cells/mm3) and leukocytosis (29.65 × 103 cells/mm3) with...(AU)