Association of Palm gene mutations with structure and function of paralemmin proteins in Lori-Bakhtiari and Zel sheep Breeds

The aim of current study was to survey genetic variability of PALM gene'sexon 3 and 4 by PCR-SSCP and DNA sequencing in Zel and Lori Bakhtiari sheep breeds. TheSIFT(Sorting Intolerant from Tolerant) and PHyre2 program were used to predict the possible impact of amino acid substitutions on performance and structure of the paralemmin protein. A total of 140 animal's from 2 Iranian sheep breeds with different fat metabolisms, Lori-Bakhtiari and Zel sheep breeds were considered. The results showed that there are two polymorphic sites including a nonsynonymous substitution and an insertion mutation (49bp). Non-synonymous mutation deduced Thr20Ala amino acid exchange and ensuing two different structures for paralemmin protein that could be potentially affect protein structure and function during the interaction with glutamate in the cytosolic surface of plasma membrane. PALMgene, according to evolutionary path, is classified into two separate categories. In first covey, Gallus gallusand in second one, other species in several branches, so that the sequence of cow and sheep is placed in a sub-branch which forms a clade beside goat. Comparison of illustrated coding region sequences, PALMgene among different species, is of orthologous which are derived from a common ancestor.(AU)

The effect of diets supplemented with Coriandrum sativumseeds on carcass performance, immune system, blood metabolites, rumen parameters and meat quality of lambs

A trial was conducted to investigate the effects of dietary Coriandrum sativum seeds on carcass performance, immune system, blood metabolites, rumen parameters and meat quality of Lambs. 16 Sanjabi lambs of 27 ± 5.1 kg during post-weaning (97 d of age) period were randomly selected. Four diets including 0, 1, 3, and 5% coriander seeds, replaced by Alfa alfa in the diet, were considered. A 30:70 alfalfa hay: concentrate diet for a period of time (97 to 187 d of age) was used. The results showed that feed intake was significantly increased by adding coriander seeds, linearly. There was no significant difference for apparent digestibility of crude protein, crude fat, neutral and acidic detergent fiber, crude ash, rumen fluid pH and ammonia nitrogen at 0, 2 and 4 h after feeding, Meat dry matter, ash, crude protein and fat, and the meats fatty acid profile (p > 0.05). Dietary coriander seeds had a significant effect on neutrophils, lymphocytes, monocytes and eosinophils (in days 7 and 14 of trial) and blood metabolites at the middle of trial. Obtained results suggested that supplementation of coriander seed may have limited effects on nutrient digestibility, ruminal parameters, meat quality, blood cells and metabolites.(AU)

The effect of diets supplemented with Coriandrum sativumseeds on carcass performance, immune system, blood metabolites, rumen parameters and meat quality of lambs

A trial was conducted to investigate the effects of dietary Coriandrum sativum seeds on carcass performance, immune system, blood metabolites, rumen parameters and meat quality of Lambs. 16 Sanjabi lambs of 27 ± 5.1 kg during post-weaning (97 d of age) period were randomly selected. Four diets including 0, 1, 3, and 5% coriander seeds, replaced by Alfa alfa in the diet, were considered. A 30:70 alfalfa hay: concentrate diet for a period of time (97 to 187 d of age) was used. The results showed that feed intake was significantly increased by adding coriander seeds, linearly. There was no significant difference for apparent digestibility of crude protein, crude fat, neutral and acidic detergent fiber, crude ash, rumen fluid pH and ammonia nitrogen at 0, 2 and 4 h after feeding, Meat dry matter, ash, crude protein and fat, and the meat’s fatty acid profile (p > 0.05). Dietary coriander seeds had a significant effect on neutrophils, lymphocytes, monocytes and eosinophil’s (in days 7 and 14 of trial) and blood metabolites at the middle of trial. Obtained results suggested that supplementation of coriander seed may have limited effects on nutrient digestibility, ruminal parameters, meat quality, blood cells and metabolites.

Genetic analysis of reproductive characteristics in Iran-Black sheep

This study was conducted to estimate genetic parameters and trends for reproduction traits using data collected at the breeding station of Iran-Black sheep during 1980 to 2004. The traits included in the analyses were litter size at birth (LSB) and weaning (LSW) and litter mean weight per lamb born (LMWLB) and weaned (LMWLW) as basic traits, and total litter weight at birth (TLWB) and weaning (TLWW) as composite traits. Direct heritability estimates for LSB, TLWB, LMWLB, LSW, TLWW and LMWLW were 0.11, 0.07, 0.33, 0.08, 0.09 and 0.11, respectively. The permanent environmental effects had significant impact on all traits and ranged from 0.05 to 0.16. Effect of service sire was highly significant (p < 0.01) for all traits except LMWLW. Estimates of genetic correlations ranged from -0.76 (LSB-LMWLB) to 0.98 (LSB-LSW). Phenotypic and environmental correlations were generally lower than those of genetic correlations. Environmental correlations ranged from -0.55 (LSW-LMWLW) to 0.99 (LSB-LSW). Also, the estimated correlation for the effect of service sire ranged from -0.77 (LMWLB-TLWW) to 0.96 (LSB-LSW and LSB-TLWW). The results suggest that selection based on TLWB could be more effective than the other traits to enhance reproductive performance in Iran-Black ewes.(AU)

Genetic analysis of reproductive characteristics in Iran-Black sheep

This study was conducted to estimate genetic parameters and trends for reproduction traits using data collected at the breeding station of Iran-Black sheep during 1980 to 2004. The traits included in the analyses were litter size at birth (LSB) and weaning (LSW) and litter mean weight per lamb born (LMWLB) and weaned (LMWLW) as basic traits, and total litter weight at birth (TLWB) and weaning (TLWW) as composite traits. Direct heritability estimates for LSB, TLWB, LMWLB, LSW, TLWW and LMWLW were 0.11, 0.07, 0.33, 0.08, 0.09 and 0.11, respectively. The permanent environmental effects had significant impact on all traits and ranged from 0.05 to 0.16. Effect of service sire was highly significant (p < 0.01) for all traits except LMWLW. Estimates of genetic correlations ranged from -0.76 (LSB-LMWLB) to 0.98 (LSB-LSW). Phenotypic and environmental correlations were generally lower than those of genetic correlations. Environmental correlations ranged from -0.55 (LSW-LMWLW) to 0.99 (LSB-LSW). Also, the estimated correlation for the effect of service sire ranged from -0.77 (LMWLB-TLWW) to 0.96 (LSB-LSW and LSB-TLWW). The results suggest that selection based on TLWB could be more effective than the other traits to enhance reproductive performance in Iran-Black ewes.