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1.
Acta sci. vet. (Online) ; 44: 01-11, 2016. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: vti-722741

Resumo

Background: There are few studies on stem cell isolation in wild animals that provide isolation and culture protocols of these cells in vitro. Among the wild species studied, we present the collared peccary (Tayassu tajacu) as a model with potential to obtain and use MSC in preclinical studies. These animals are phylogenetically close to the domestic pig, popularly known as peccaries and found naturally in South America, Central America and the South of the United States. The aim of the present study was to establish a protocol for the isolation, in vitro cell expansion, differentiation and assessment of the stromal MSC growth curve before and after thawing. Materials, Methods & Results: Mesenchymal stem cells (MSC) from collared peccary bone marrow (Tayassu tajacu) were isolated and expanded by centrifuge in Ficoll® solution and cultured in DMEM® High Glucose medium. The culture was assessed by assays of colony forming units CFU-F and growth curve by saturation (GCS). Cultures in the third passage, with 70% confluence, were replicated at 105 cells/mL concentration in the culture media to induce osteogenic cell differentiation and adipogenic cell differentiation, respectively. The MSC were frozen in nitrogen for 40 days, thawed and re-assessed for cell viability and GCS. Discussion: The bone marrow collected presented high mononuclear cellularity, with a mean variability of 94.5% and 60.83 ± 4.27 UFC were identified in the samples and cells with fibroblast-like-cell morphology were observed. When they were expanded, the mean cell viability was 95%, the mean cell concentration obtained was 233.31 ± 20.04 cells per 25cm2 bottle and the culture reached the growth plateau in GCS between the 13th and 16th day. The osteoblastic cell differentiation assay showed after 18 days, morphology similar to osteoblasts, with irregular cytoplasm limits, cell prolongation formation and flattened appearance. [...](AU)


Assuntos
Animais , Artiodáctilos , Células-Tronco Mesenquimais , Células da Medula Óssea , Terapia Baseada em Transplante de Células e Tecidos/veterinária , Separação Celular/normas , Adipogenia , Criopreservação/veterinária , Modelos Animais
2.
Acta sci. vet. (Impr.) ; 44: 01-11, 2016. ilus, tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1457471

Resumo

Background: There are few studies on stem cell isolation in wild animals that provide isolation and culture protocols of these cells in vitro. Among the wild species studied, we present the collared peccary (Tayassu tajacu) as a model with potential to obtain and use MSC in preclinical studies. These animals are phylogenetically close to the domestic pig, popularly known as peccaries and found naturally in South America, Central America and the South of the United States. The aim of the present study was to establish a protocol for the isolation, in vitro cell expansion, differentiation and assessment of the stromal MSC growth curve before and after thawing. Materials, Methods & Results: Mesenchymal stem cells (MSC) from collared peccary bone marrow (Tayassu tajacu) were isolated and expanded by centrifuge in Ficoll® solution and cultured in DMEM® High Glucose medium. The culture was assessed by assays of colony forming units CFU-F and growth curve by saturation (GCS). Cultures in the third passage, with 70% confluence, were replicated at 105 cells/mL concentration in the culture media to induce osteogenic cell differentiation and adipogenic cell differentiation, respectively. The MSC were frozen in nitrogen for 40 days, thawed and re-assessed for cell viability and GCS. Discussion: The bone marrow collected presented high mononuclear cellularity, with a mean variability of 94.5% and 60.83 ± 4.27 UFC were identified in the samples and cells with fibroblast-like-cell morphology were observed. When they were expanded, the mean cell viability was 95%, the mean cell concentration obtained was 233.31 ± 20.04 cells per 25cm2 bottle and the culture reached the growth plateau in GCS between the 13th and 16th day. The osteoblastic cell differentiation assay showed after 18 days, morphology similar to osteoblasts, with irregular cytoplasm limits, cell prolongation formation and flattened appearance. [...]


Assuntos
Animais , Artiodáctilos , Células da Medula Óssea , Células-Tronco Mesenquimais , Adipogenia , Criopreservação/veterinária , Modelos Animais , Separação Celular/normas , Terapia Baseada em Transplante de Células e Tecidos/veterinária
3.
Semina Ci. agr. ; 35(4): 1847-1854, July.-Aug.2014. tab
Artigo em Português | VETINDEX | ID: vti-26545

Resumo

Non-human primates serve as a model for studies on the dynamics of transmission routes and natural history of diseases shared between humans and animals. The captive environment is conducive the dissemination of zoonotic diseases. Many of these animals do not present clinical symptomatology, even when infected, which characterize them as an important source of infection for domestic animals and man. The objective of this study was to evaluate the sanity of non-human primates kept in captivity through hematological and biochemical analysis as well as peripheral blood smear, aiming to investigate the presence of zoonotic pathogens, serving as a model for future studies on the dynamics of routes of transmission of diseases shared between humans and animals. Were collected samples of blood from 15 nail monkeys (Cebus sp.), adults, clinically healthy and belonging to Park Zoobotanic of Teresina. Were stained smear sanguine blades and obtained the haematological and biochemical profiles of each animal. The data analysis was based on basic statistics. Did not observed any haemoparasite present in peripheral blood. All animals were anemic, 46,7% thrombopenics and 87% of the animals showed some type of pathological process of chronic evolution, due to the high rate of monocytes found. All animals showed high rates of alkaline phosphatase, and transaminases AST and ALT, indicating injury of the hepatic parenchyma. New studies should be conducted to better elucidate of results, seeing that biochemical physiological data primate of the genus Cebus are scarce in literature.(AU)


Os primatas não humanos atuam como modelo para estudos sobre a dinâmica das vias de transmissão e história natural de doenças compartilhadas entre homens e animais. O ambiente de cativeiro é propício à disseminação de doenças de caráter zoonótico. Muitos destes animais não apresentam sintomatologia clínica, mesmo estando infectados, o que os caracterizam como importante fonte de infecção para os animais domésticos e o homem. O objetivo deste trabalho foi avaliar a sanidade de primatas não humanos mantidos em cativeiro através de análise hematológica e bioquímica, bem como esfregaço de sangue periférico, com intuito de investigar a presença de patógenos de caráter zoonótico, servindo de modelo para estudos futuros sobre a dinâmica das vias de transmissão de doenças compartilhadas entre homens e animais. Foram coletadas amostras de sangue de 15 macacos prego (Cebus sp.), adultos, clinicamente saudáveis e pertencentes ao Parque Zoobotânico de Teresina. Foram coradas lâminas de esfregaço sanguíneo e obtidos os perfis hematológicos e bioquímicos de cada animal. A análise dos dados baseou-se em estatística básica. Não foi observado nenhum hemoparasita presente em sangue periférico. Todos os animais estavam anêmicos, 46,7% trombopênicos e 87% apresentavam algum tipo de processo patológico de evolução crônica, devido à elevada taxa de monócitos encontrados. Todos os animais apresentaram elevadas taxas de fosfatase alcalina e das transaminases AST e ALT, indicando injúria do parênquima hepático. Novos estudos deverão ser realizados para melhor elucidação dos resultados, visto que dados bioquímicos fisiológicos de primatas do gênero Cebus são escassos na literatura.(AU)


Assuntos
Animais , Cebus/sangue , Anemia , Fosfatase Alcalina , Transaminases , Trombocitopenia , Testes Hematológicos/veterinária , Zoonoses
4.
Acta cir. bras. ; 29(9): 560-572, Sept. 2014. ilus, graf, tab
Artigo em Inglês | VETINDEX | ID: vti-21947

Resumo

PURPOSE:The use of the collared peccary as an experimental model for ischemic nephropathy.METHODS:A total of 12 collared peccary (Tayassu tajacu) was used and ischemic nephropathy was induced in six of these animals that constituted the experimental group (G1) while the other six formed the control group (G2). Ischemic nephropathy was induced surgically by partial occlusion of the left renal artery. The disease course was assessed by hematological tests, serum chemistry, urinalysis, ultrasound (US) and doppler ultrasound function of the renal artery before induction, and at five, 10, 15 and 20 days after surgery. Twenty days after the occlusion, unilateral nephrectomy and histopathological examination were performed to assess renal morphology.RESULTS:Statistical analysis by Fischer's test showed a significant difference (p<0.05) between the control group and the experimental group. The histopathological examination showed glomerular, tubular and interstitial lesions. In the experimental group, 83.3% (5 /6) showed moderate renal lesions and only 16.7% (1/6) were classified with no lesions. The ultrasound examination of the right kidney presented statistical difference between day 5 and day 10 post occlusion.CONCLUSION:The collared peccary as a good experimental model for ischemic renal disease, because it could be manipulated during the research time without death, with health conditions that permit any subsequent procedure for disease therapy.(AU)


Assuntos
Animais , Artiodáctilos/anatomia & histologia , Nefropatias/veterinária , Isquemia/veterinária , Artéria Renal , Modelos Animais de Doenças
5.
Acta cir. bras. ; 29(8): 478-484, 08/2014. graf, ilus
Artigo em Inglês | VETINDEX | ID: vti-13048

Resumo

PURPOSE: To characterize bone marrow progenitors cells grown in vitro, using native goats from northeastern Brazil as animal model. METHODS: Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog). RESULTS: Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog. CONCLUSIONS: Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.(AU)


Assuntos
Animais , Cabras , Medula Óssea , Células-Tronco/classificação , Pesquisa com Células-Tronco , Citometria de Fluxo/veterinária , Técnicas de Cultura de Células , Antígeno Nuclear de Célula em Proliferação/análise
6.
Semina ciênc. agrar ; 35(4): 1847-1854, 2014. tab
Artigo em Português | VETINDEX | ID: biblio-1499667

Resumo

Non-human primates serve as a model for studies on the dynamics of transmission routes and natural history of diseases shared between humans and animals. The captive environment is conducive the dissemination of zoonotic diseases. Many of these animals do not present clinical symptomatology, even when infected, which characterize them as an important source of infection for domestic animals and man. The objective of this study was to evaluate the sanity of non-human primates kept in captivity through hematological and biochemical analysis as well as peripheral blood smear, aiming to investigate the presence of zoonotic pathogens, serving as a model for future studies on the dynamics of routes of transmission of diseases shared between humans and animals. Were collected samples of blood from 15 nail monkeys (Cebus sp.), adults, clinically healthy and belonging to Park Zoobotanic of Teresina. Were stained smear sanguine blades and obtained the haematological and biochemical profiles of each animal. The data analysis was based on basic statistics. Did not observed any haemoparasite present in peripheral blood. All animals were anemic, 46,7% thrombopenics and 87% of the animals showed some type of pathological process of chronic evolution, due to the high rate of monocytes found. All animals showed high rates of alkaline phosphatase, and transaminases AST and ALT, indicating injury of the hepatic parenchyma. New studies should be conducted to better elucidate of results, seeing that biochemical physiological data primate of the genus Cebus are scarce in literature.


Os primatas não humanos atuam como modelo para estudos sobre a dinâmica das vias de transmissão e história natural de doenças compartilhadas entre homens e animais. O ambiente de cativeiro é propício à disseminação de doenças de caráter zoonótico. Muitos destes animais não apresentam sintomatologia clínica, mesmo estando infectados, o que os caracterizam como importante fonte de infecção para os animais domésticos e o homem. O objetivo deste trabalho foi avaliar a sanidade de primatas não humanos mantidos em cativeiro através de análise hematológica e bioquímica, bem como esfregaço de sangue periférico, com intuito de investigar a presença de patógenos de caráter zoonótico, servindo de modelo para estudos futuros sobre a dinâmica das vias de transmissão de doenças compartilhadas entre homens e animais. Foram coletadas amostras de sangue de 15 macacos prego (Cebus sp.), adultos, clinicamente saudáveis e pertencentes ao Parque Zoobotânico de Teresina. Foram coradas lâminas de esfregaço sanguíneo e obtidos os perfis hematológicos e bioquímicos de cada animal. A análise dos dados baseou-se em estatística básica. Não foi observado nenhum hemoparasita presente em sangue periférico. Todos os animais estavam anêmicos, 46,7% trombopênicos e 87% apresentavam algum tipo de processo patológico de evolução crônica, devido à elevada taxa de monócitos encontrados. Todos os animais apresentaram elevadas taxas de fosfatase alcalina e das transaminases AST e ALT, indicando injúria do parênquima hepático. Novos estudos deverão ser realizados para melhor elucidação dos resultados, visto que dados bioquímicos fisiológicos de primatas do gênero Cebus são escassos na literatura.


Assuntos
Animais , Anemia , Cebus/sangue , Fosfatase Alcalina , Transaminases , Trombocitopenia , Testes Hematológicos/veterinária , Zoonoses
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