Testicular and seminal evaluation of dogs naturally infected with Leishmania sp / Avaliação testicular e seminal de cães naturalmente infectados por Leishmania sp

The objective of this study was to evaluate the testicular changes and detect the presence of Leishmania sp. in the testicles and semen of dogs with Visceral Leishmaniasis (VL). The animals were obtained from the Zoonoses Control Department of Teresina, PI, and taken to the kennel of the Agricultural Sciences Center of the Federal University of Piauí, where they remained were maintained for two months and subsequently euthanized for testicles removal. Semen samples were collected from 12 dogs, 06 positive and 06 negative for VL. The following diagnostic techniques readouts were assessed for the sampled animals: testosterone dosage, immunohistochemistry (IMH), histopathology of the slides containing the testicular material, and seminal evaluation by polymerase chain reaction (PCR). Testosterone values remained within the normal range for the canine specie and did not differ statistically among the experimental groups but displayed lower serum concentrations than those of the control group. All the testicular and semen samples from the dogs were negative for VL as determined by techniques IMH and PCR, respectively. The results of testicle histopathology revealed the presence of several lesions with statistical difference among the experimental groups. Parasitized dogs with VL have testicular lesions that may compromise the reproductive efficiency of these animals.(AU)

O objetivo deste estudo foi avaliar as alterações testiculares e detectar a presença de Leishmania sp. nos testículos e sêmen de cães com leishmaniose visceral (LV). Os animais foram obtidos da Gerência de Controle de Zoonoses de Teresina, PI, e levados ao canil do Centro de Ciências Agrárias da Universidade Federal do Piauí, onde permaneceram por dois meses e posteriormente foram submetidos à eutanásia para a remoção dos testículos. As amostras de sêmen foram coletadas de 12 cães, sendo 06 positivos e 06 negativos para LV. Nas amostras dos animais, foram realizadas as seguintes técnicas de diagnóstico: dosagem de testosterona, imunohistoquímica (IMH), histopatologia das lâminas contendo o material testicular e avaliação seminal por reação em cadeia pela polimerase (PCR). Valores de testosterona permaneceram dentro do intervalo normal para a espécie canina, não diferindo estatisticamente entre os grupos experimentais, porém apresentando concentrações séricas mais baixas do que o grupo controle. Todas as amostras testiculares e de sêmen dos cães foram negativas para LV, conforme determinado pelas técnicas IMH e PCR, respectivamente. Os resultados da histopatologia dos testículos revelaram a presença de várias lesões com diferença estatística entre os grupos experimentais. Os cães parasitados com LV apresentam lesões testiculares que podem comprometer a eficiência reprodutiva desses animais.(AU)

Ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí, Brazil / Oftalmopatias em cães naturalmente acometidos por leishmaniose visceral em Teresina, PI, Brasil

This study aimed to characterize ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí State, Brazil. The diagnosis was made using parasitological exams of the bone-marrow and lymph-node samples. The main ophthalmological findings were uveitis, conjunctivitis, blepharitis, keratitis, and keratoconjunctivitis sicca. Normocytic normochromic anemia was the main hematological finding, followed by thrombocytopenia. Plasma proteins were also considered, and hyperproteinemia, hypergammaglobulinemia, and hypoalbuminemia were observed. Ocular histopathological examination revealed mild inflammation involving lymphocytes, monocytes, and macrophages. Results indicate the need to perform a differential diagnosis to rule out or establish the presence of Leishmania sp. in dogs presenting with ophthalmic lesions in endemic regions.

O presente trabalho teve por objetivo caracterizar as oftalmopatias em cães naturalmente afetados por leishmaniose visceral em Teresina, Piauí. O diagnóstico de leishmaniose foi realizado por exame parasitológico de medula óssea e linfonodos. As principais alterações oftálmicas foram uveíte, conjuntivite, blefarite, ceratite e ceratoconjuntivite seca. Anemia normocítica normocrômica foi a principal alteração hematológica, seguida de trombocitopenia. Em relação às proteínas plasmáticas observou-se hiperproteinemia, hiperglobulinemia e hipoalbuminemia. A histopatologia ocular revelou processo inflamatório moderado constituído por linfócitos, monócitos, neutrófilos e macrófagos. Os resultados da pesquisa apontam para a necessidade de se realizar diagnóstico diferencial para Leishmania sp. em cães que apresentem lesões oftálmicas em regiões endêmicas.

Ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí, Brazil / Oftalmopatias em cães naturalmente acometidos por leishmaniose visceral em Teresina, PI, Brasil

This study aimed to characterize ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí State, Brazil. The diagnosis was made using parasitological exams of the bone-marrow and lymph-node samples. The main ophthalmological findings were uveitis, conjunctivitis, blepharitis, keratitis, and keratoconjunctivitis sicca. Normocytic normochromic anemia was the main hematological finding, followed by thrombocytopenia. Plasma proteins were also considered, and hyperproteinemia, hypergammaglobulinemia, and hypoalbuminemia were observed. Ocular histopathological examination revealed mild inflammation involving lymphocytes, monocytes, and macrophages. Results indicate the need to perform a differential diagnosis to rule out or establish the presence of Leishmania sp. in dogs presenting with ophthalmic lesions in endemic regions.(AU)

O presente trabalho teve por objetivo caracterizar as oftalmopatias em cães naturalmente afetados por leishmaniose visceral em Teresina, Piauí. O diagnóstico de leishmaniose foi realizado por exame parasitológico de medula óssea e linfonodos. As principais alterações oftálmicas foram uveíte, conjuntivite, blefarite, ceratite e ceratoconjuntivite seca. Anemia normocítica normocrômica foi a principal alteração hematológica, seguida de trombocitopenia. Em relação às proteínas plasmáticas observou-se hiperproteinemia, hiperglobulinemia e hipoalbuminemia. A histopatologia ocular revelou processo inflamatório moderado constituído por linfócitos, monócitos, neutrófilos e macrófagos. Os resultados da pesquisa apontam para a necessidade de se realizar diagnóstico diferencial para Leishmania sp. em cães que apresentem lesões oftálmicas em regiões endêmicas.(AU)

Histopatologia e Imunohistoquímica de testículos de cães naturalmente infectados por Leishmania sp / Histopathology and Immunohistochemistry testicles of dogs naturally infected by Leishmania sp

The objective of this study was to evaluate testicular changes and the presence of Leishmania sp. intesticles of dogs with Visceral Leishmaniasis (LV). The animals were obtained from Teresina Zoonosis ControlAdministration, and taken to the kennels of Agricultural Sciences Center of the Federal University of Piauí(UFPI), where they stayed for two months and were subsequently euthanized for removal of the testicles. 12 dogswere used, 06 positive (GI) and 06 negative (GII) for LV and conducted examinations of histopathology andimmunohistochemistry (IMH) of the testicles. All were negative by IMH technique and by histopathology. In thisstudy, we observed testicular lesions in all animals, with no direct relationship between the presence ofLeishmania sp. and intensity of the damage found in the reproductive system, reducing the possibility of venerealtransmission between oligositomatics dogs.(AU)

Histopatologia e Imunohistoquímica de testículos de cães naturalmente infectados por Leishmania sp / Histopathology and Immunohistochemistry testicles of dogs naturally infected by Leishmania sp

The objective of this study was to evaluate testicular changes and the presence of Leishmania sp. intesticles of dogs with Visceral Leishmaniasis (LV). The animals were obtained from Teresina Zoonosis ControlAdministration, and taken to the kennels of Agricultural Sciences Center of the Federal University of Piauí(UFPI), where they stayed for two months and were subsequently euthanized for removal of the testicles. 12 dogswere used, 06 positive (GI) and 06 negative (GII) for LV and conducted examinations of histopathology andimmunohistochemistry (IMH) of the testicles. All were negative by IMH technique and by histopathology. In thisstudy, we observed testicular lesions in all animals, with no direct relationship between the presence ofLeishmania sp. and intensity of the damage found in the reproductive system, reducing the possibility of venerealtransmission between oligositomatics dogs.

Testicular and seminal evaluation of dogs naturally infected with Leishmania sp / Avaliação testicular e seminal de cães naturalmente infectados por Leishmania sp

The objective of this study was to evaluate the testicular changes and detect the presence of Leishmania sp. in the testicles and semen of dogs with Visceral Leishmaniasis (VL). The animals were obtained from the Zoonoses Control Department of Teresina, PI, and taken to the kennel of the Agricultural Sciences Center of the Federal University of Piauí, where they remained were maintained for two months and subsequently euthanized for testicles removal. Semen samples were collected from 12 dogs, 06 positive and 06 negative for VL. The following diagnostic techniques readouts were assessed for the sampled animals: testosterone dosage, immunohistochemistry (IMH), histopathology of the slides containing the testicular material, and seminal evaluation by polymerase chain reaction (PCR). Testosterone values remained within the normal range for the canine specie and did not differ statistically among the experimental groups but displayed lower serum concentrations than those of the control group. All the testicular and semen samples from the dogs were negative for VL as determined by techniques IMH and PCR, respectively. The results of testicle histopathology revealed the presence of several lesions with statistical difference among the experimental groups. Parasitized dogs with VL have testicular lesions that may compromise the reproductive efficiency of these animals.

O objetivo deste estudo foi avaliar as alterações testiculares e detectar a presença de Leishmania sp. nos testículos e sêmen de cães com leishmaniose visceral (LV). Os animais foram obtidos da Gerência de Controle de Zoonoses de Teresina, PI, e levados ao canil do Centro de Ciências Agrárias da Universidade Federal do Piauí, onde permaneceram por dois meses e posteriormente foram submetidos à eutanásia para a remoção dos testículos. As amostras de sêmen foram coletadas de 12 cães, sendo 06 positivos e 06 negativos para LV. Nas amostras dos animais, foram realizadas as seguintes técnicas de diagnóstico: dosagem de testosterona, imunohistoquímica (IMH), histopatologia das lâminas contendo o material testicular e avaliação seminal por reação em cadeia pela polimerase (PCR). Valores de testosterona permaneceram dentro do intervalo normal para a espécie canina, não diferindo estatisticamente entre os grupos experimentais, porém apresentando concentrações séricas mais baixas do que o grupo controle. Todas as amostras testiculares e de sêmen dos cães foram negativas para LV, conforme determinado pelas técnicas IMH e PCR, respectivamente. Os resultados da histopatologia dos testículos revelaram a presença de várias lesões com diferença estatística entre os grupos experimentais. Os cães parasitados com LV apresentam lesões testiculares que podem comprometer a eficiência reprodutiva desses animais.