Transferência de embriões como ferramenta para formação de rebanho experimental ovino e análise dos fatores que podem maximizar a multiplicação do plantel / Embryo transfer as a tool to experimental sheep flock formation and stock multiplication factors analyses

Visando ao aumento do plantel de ovinos da raça Black Belly na estação experimental do Institut Scientifique de Recherche Agronomique (INRA) de Bourges, França, foram utilizadas 32 ovelhas da raça Black Belly superovuladas com FSH suíno nos últimos três dias de tratamento progestágeno de 14 dias. Após coleta cirúrgica, obtiveram-se 264 embriões transferíveis (8,2 ± 6,0/doadoras), sendo transferidos 232 a 88 receptoras da raça Romane pluríparas e 23 da raça Suffolk nulíparas. As taxas de parição e de sobrevivência embrionária foram 83,6 e 63%, respectivamente, sendo os melhores resultados observados em receptoras da raça Romane pluríparas do que nas nulíparas da raça Suffolk. O número de ovulações, de embriões transferidos e o estádio de desenvolvimento embrionário causaram efeito sobre a taxa de sobrevivência embrionária. As melhores taxas de parição e de sobrevivência embrionária foram obtidas de receptoras com mais de duas ovulações que receberam dois embriões no estádio de blastocisto.(AU)

With the purpose of increase the breeding of Black Belly sheep breed at the experimental station of the Institut Scientifique de Recherche Agronomique (INRA), Bourges, France, thirty-two Black Belly sheep were super ovulated with swine FSH on the last three days of a progesterone treatment. After surgical collect 264 embryos transferable were recovered (8.2 ± 6.0/ donor) and 232 were transferred to 88 multiparous Romane sheep receptors and 23 nulliparous Suffolk sheep. Birth rate and survival rate were 83.6 and 63% respectively, though the results form multiparous Romane sheep receptors were better than from nulliparous Suffolk sheep. The ovulations number, transferred embryos and development stage had an effect on embryo survival rate. The best birth and embryo survival rates were from receptors that had more than two ovulations and had two embryos implanted at the stage of blastocyst.(AU)

Transferência de embriões como ferramenta para formação de rebanho experimental ovino e análise dos fatores que podem maximizar a multiplicação do plantel / Embryo transfer as a tool to experimental sheep flock formation and stock multiplication factors analyses

Visando ao aumento do plantel de ovinos da raça Black Belly na estação experimental do Institut Scientifique de Recherche Agronomique (INRA) de Bourges, França, foram utilizadas 32 ovelhas da raça Black Belly superovuladas com FSH suíno nos últimos três dias de tratamento progestágeno de 14 dias. Após coleta cirúrgica, obtiveram-se 264 embriões transferíveis (8,2 ± 6,0/doadoras), sendo transferidos 232 a 88 receptoras da raça Romane pluríparas e 23 da raça Suffolk nulíparas. As taxas de parição e de sobrevivência embrionária foram 83,6 e 63%, respectivamente, sendo os melhores resultados observados em receptoras da raça Romane pluríparas do que nas nulíparas da raça Suffolk. O número de ovulações, de embriões transferidos e o estádio de desenvolvimento embrionário causaram efeito sobre a taxa de sobrevivência embrionária. As melhores taxas de parição e de sobrevivência embrionária foram obtidas de receptoras com mais de duas ovulações que receberam dois embriões no estádio de blastocisto.

With the purpose of increase the breeding of Black Belly sheep breed at the experimental station of the Institut Scientifique de Recherche Agronomique (INRA), Bourges, France, thirty-two Black Belly sheep were super ovulated with swine FSH on the last three days of a progesterone treatment. After surgical collect 264 embryos transferable were recovered (8.2 ± 6.0/ donor) and 232 were transferred to 88 multiparous Romane sheep receptors and 23 nulliparous Suffolk sheep. Birth rate and survival rate were 83.6 and 63% respectively, though the results form multiparous Romane sheep receptors were better than from nulliparous Suffolk sheep. The ovulations number, transferred embryos and development stage had an effect on embryo survival rate. The best birth and embryo survival rates were from receptors that had more than two ovulations and had two embryos implanted at the stage of blastocyst.

Oocytoe production and in vitro maturation in Canindé goats following hormonal ovarian stimulation

This study e valuated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus - oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro matur ation (IVM) . Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg m edroxyprogesterone acetate for 11 days, together with a n injection of 50 μg d - cloprostenol on the 8 th day , along with additional treatment re g imens , as follows : i) five doses (5D), 120 mg of NIH - FSH - P1 in five injections at 12 h inter vals; ii) three doses (3D), 120 mg of NIH - FSH - P1 in three injections at 24 h i nterv als; iii) single dose (1D), 70 mg of NIH - FSH - P 1 and 200 IU of eCG at 36 h prior to sponge rem oval. Three sessions of hormonal treatment/oocyte recover y w ere performed and goats ( n = 6 / group) were allocated to different grou ps . The oocytes were collect ed by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being rec overed , resulting in a recovery rate of 74.7 % ( 577/772 ) . The mean number of CO Cs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). R ecovery rate was higher (P < 0.05) in the 5 D group ( 84.1 % ; 211/251 ) compared to the 3 D ( 68.2 % ; 182/267 ) and 1D group s (72.4% ; 184/254 ). T he lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1% ; 27/84 ), while the rate for the 5D and 1D groups was 49.1% ( 53/108 ) and 46.2% ( 42/91 ) , respectively. Finally, taking into account the main performance results of th e three treatments, it i s advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes .

Oocytoe production and in vitro maturation in Canindé goats following hormonal ovarian stimulation

This study e valuated the effect of hormonal ovarian stimulation regimes on the quantity and quality of the cumulus - oocyte complexes (COCs) recovered by laparoscopy and their subsequent in vitro matur ation (IVM) . Eighteen cyclic Canindé goats received a vaginal sponge with 60 mg m edroxyprogesterone acetate for 11 days, together with a n injection of 50 μg d - cloprostenol on the 8 th day , along with additional treatment re g imens , as follows : i) five doses (5D), 120 mg of NIH - FSH - P1 in five injections at 12 h inter vals; ii) three doses (3D), 120 mg of NIH - FSH - P1 in three injections at 24 h i nterv als; iii) single dose (1D), 70 mg of NIH - FSH - P 1 and 200 IU of eCG at 36 h prior to sponge rem oval. Three sessions of hormonal treatment/oocyte recover y w ere performed and goats ( n = 6 / group) were allocated to different grou ps . The oocytes were collect ed by laparoscopy at the time of sponge removal and the IVM of the oocytes was monitored. A total of 14.8 ± 0.8 follicles were aspirated per animal with 11.1 ± 0.6 COCs being rec overed , resulting in a recovery rate of 74.7 % ( 577/772 ) . The mean number of CO Cs/goat did not differ (P > 0.05) between treatments (11.7 ± 1.1, 10.7 ± 0.9 and 10.8 ± 1.0 for the 5D, 3D and 1D groups, respectively). R ecovery rate was higher (P < 0.05) in the 5 D group ( 84.1 % ; 211/251 ) compared to the 3 D ( 68.2 % ; 182/267 ) and 1D group s (72.4% ; 184/254 ). T he lowest (P < 0.05) maturation rate was recorded in the 3D group (32.1% ; 27/84 ), while the rate for the 5D and 1D groups was 49.1% ( 53/108 ) and 46.2% ( 42/91 ) , respectively. Finally, taking into account the main performance results of th e three treatments, it i s advisable to use the 5D regime in future Canindé breed preservation programs based on laparoscopy technology as a means to recover oocytes .(AU)