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1.
Arq. bras. med. vet. zootec ; 64(6): 1665-1672, 2012. ilus, tab
Artigo em Português | VETINDEX | ID: vti-10884

Resumo

Identificou-se o efeito das aflatoxinas (AFs) sobre o gene p53 de frangos de corte, de linhagem comercial, separados em: grupo experimental, tratado (GT) com ração comercial contendo 2,8ppm de AFs totais durante 21 dias consecutivos, e grupo-controle (GC), sem exposição às AFs. Macroscopicamente, as alterações caracterizaram-se por hepatomegalia e aspecto pálido-amarelado com alguns focos hemorrágicos e, histologicamente, por desarranjo trabecular, pleomorfismo hepatocítico com cariomegalia, degeneração vacuolar intracitoplasmática, necrose com infiltração linfocítica e hiperplasia de ductos biliares. A PCR com os primers GSPT53c-1 com base no gene candidato a p53 (GenBank XM_424937.2) gerou um produto de aproximadamente 350 pares de base. O amplicon sequenciado a partir do DNA dos frangos do GT não apresentou mutação ou deleção, assim como padrão de bandas do PCR-RFLP não foi distinto entre ambos os grupos experimentais e a sequência depositada no banco de genes. Os resultados sugerem que não ocorreu transversão devido à exposição às AFs no fragmento amplificado. Conclui-se que a PCR-RFLP e o sequenciamento do produto da PCR não são ferramentas apropriadas para diagnóstico da exposição de frangos às AFs nas condições experimentais empregadas.(AU)


To identify the effects of aflatoxins (AFs), Cobb lineage poultry were separated in an experimental group in which they were treated with commercial ration containing 2.8ppm of total AFs during 21 days (TG) and a control group without AFs exposure (CG). In the liver of poultries exposed to AFs, alterations were microscopically observed, which were characterized by hepatomegaly, a pale yellowish aspect with some hemorrhagic spots, and histologically a trabecullar disarranging pleomorphic hepatocytes with cariomegaly, intracytoplasmatic vacuolar degeneration, necrosis, lymphocytic infiltration and hyperplasia of biliary ducts. The PCR with GSPT53c-1 primers based on p53 candidate gen (GenBank XM_424937.2) generated a product of approximately 350 base pairs. The sequenced amplicon obtained from the DNA of treated poultry did not display any mutation or deletion, and the PCR- RFLP bands patterns were also not distinct in both experimental groups. The results indicated that transversion did not occur in the fragment amplified due to AFs exposure. As a consequence of results obtained with p53 gene (NM_205264.1) we concluded that PCR-RFLP and sequencing of PCR product are not appropriate diagnostic tools for the detection of poultry exposure to AFs, at least in the experimental conditions performed.(AU)


Assuntos
Animais , Aves Domésticas , Genes p53 , Aflatoxinas/efeitos adversos , Ração Animal , Hepatomegalia/veterinária , Reação em Cadeia da Polimerase
2.
Arq. Inst. Biol ; 76(3)2009.
Artigo em Português | LILACS-Express | VETINDEX | ID: biblio-1462044

Resumo

ABSTRACT The objective of this study was to determine the occurrence of aflatoxins in the corn used as poultry feed in the state of Bahia, Brazil. Eighty samples of corn were collected from two poultry feed factories between February 2005 and January 2006. The aflatoxins analyses were performed using a fluorometric technique with commercial immunoaffinity columns (Aflatest®, Vicam). The results revealed contamination in 8 (10%) of the samples, with levels varying from 1 to 5 ?g/kg. These results reflect the good quality of this product in regard to contamination by aflatoxins.


RESUMO O objetivo deste estudo foi determinar a ocorrência de aflatoxinas (AFs) em milho destinado à alimentação de aves no Estado da Bahia. Oitenta amostras de milho foram coletadas de duas fábricas produtoras de ração durante o período de fevereiro de 2005 a janeiro de 2006. As análises de AFs foram realizadas por meio da técnica de fluorimetria com colunas de imunoafinidade (Aflatest®, Vicam). Os resultados revelaram que oito (10%) amostras estavam contaminadas, com níveis variáveis de 1 a 5 ?g/kg. Estes resultados demonstram a boa qualidade do produto quanto à contaminação por aflatoxinas.

3.
Arq. bras. med. vet. zootec ; 60(2): 503-505, abr. 2008.
Artigo em Português | VETINDEX | ID: vti-6871

Resumo

The optimization of the thin layer chromatography (TLC) method to determine aflatoxins in feedstuffs and the evaluation of their occurrence in feedstuffs for milk-yielding does in the state of Bahia were studied. Eighty feedstuff samples were collected from five farms, located in the Recôncavo Baiano, from November 2000 to August 2002. The samples were analyzed using TLC modified method. In this study, the detection and quantification limits were 5 and 8m g/kg, respectively. The percentage of average recoveries obtained for AFB1, AFB2, AFG1, and AFG2 were 81.0; 97.2; 89.3; and 90.3 percent, respectively; and the coefficient of variation ranged from 0.83 to 4.1 percent. The results revealed that the optimization of TLC methodology is efficient to analyze aflatoxins in feedstuffs. The presence of these aflatoxins was not detected in any of the analyzed samples, demonstrating good quality of those products, regarding the contamination by these toxins.(AU)


Assuntos
Animais , Ração Animal , Métodos , Cabras , Aflatoxinas/análise
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