Analgesic and side effects of intravenous recombinant Phα1ß

Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1ß exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1ß in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters. Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the recombinant version of Phα1ß using Escherichia coli expression. In rats, neuropathic pain was induced by chronic constriction of the sciatic nerve and paclitaxel-induced acute and chronic pain. Mechanical sensitivity was evaluated using von Frey filaments. A radiotelemeter transmitter (TA11PA-C10; Data Sciences, St. Paul, MN, USA) was placed on the left carotid of mice for investigation of cardiovascular side effects. Locomotor activity data were evaluated using the open-field paradigm, and serum CKMB, TGO, TGP, LDH, lactate, creatinine, and urea levels were examined. Results: Intravenous administration of recombinant Phα1ß toxin induced analgesia for up to 4 h, with ED50 of 0.02 (0.01-0.03) mg/kg, and reached the maximal effect (Emax = 100% antinociception) at a dose of 0.2 mg/kg. No significant changes were observed in any of the evaluated motor, cardiac or biochemical parameters. Conclusion: Our data suggest that intravenous administration of recombinant Phα1ß may be feasible for drug-induced analgesia, without causing any severe side effects.(AU)

Analgesic and side effects of intravenous recombinant Ph alfa 1 beta

Background: Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1β exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1β in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters. Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the recombinant version of Phα1β using Escherichia coli expression. In rats, neuropathic pain was induced by chronic constriction of the sciatic nerve and paclitaxel-induced acute and chronic pain. Mechanical sensitivity was evaluated using von Frey filaments. A radiotelemeter transmitter (TA11PA-C10; Data Sciences, St. Paul, MN, USA) was placed on the left carotid of mice for investigation of cardiovascular side effects. Locomotor activity data were evaluated using the open-field paradigm, and serum CKMB, TGO, TGP, LDH, lactate, creatinine, and urea levels were examined. Results: Intravenous administration of recombinant Phα1β toxin induced analgesia for up to 4 h, with ED50 of 0.02 (0.01-0.03) mg/kg, and reached the maximal effect (Emax = 100% antinociception) at a dose of 0.2 mg/kg. No significant changes were observed in any of the evaluated motor, cardiac or biochemical parameters. Conclusion: Our data suggest that intravenous administration of recombinant Phα1β may be feasible for drug-induced analgesia, without causing any severe side effects.(AU)

Analgesic and side effects of intravenous recombinant Ph alfa 1 beta

Background: Intrathecal injection of voltage-sensitive calcium channel blocker peptide toxins exerts analgesic effect in several animal models of pain. Upon intrathecal administration, recombinant Phα1β exerts the same analgesic effects as the those of the native toxin. However, from a clinical perspective, the intrathecal administration limits the use of anesthetic drugs in patients. Therefore, this study aimed to investigate the possible antinociceptive effect of intravenous recombinant Phα1β in rat models of neuropathic pain, as well as its side effects on motor, cardiac (heart rate and blood pressure), and biochemical parameters. Methods: Male Wistar rats and male Balb-C mice were used in this study. Giotto Biotech® synthesized the recombinant version of Phα1β using Escherichia coli expression. In rats, neuropathic pain was induced by chronic constriction of the sciatic nerve and paclitaxel-induced acute and chronic pain. Mechanical sensitivity was evaluated using von Frey filaments. A radiotelemeter transmitter (TA11PA-C10; Data Sciences, St. Paul, MN, USA) was placed on the left carotid of mice for investigation of cardiovascular side effects. Locomotor activity data were evaluated using the open-field paradigm, and serum CKMB, TGO, TGP, LDH, lactate, creatinine, and urea levels were examined. Results: Intravenous administration of recombinant Phα1β toxin induced analgesia for up to 4 h, with ED50 of 0.02 (0.01-0.03) mg/kg, and reached the maximal effect (Emax = 100% antinociception) at a dose of 0.2 mg/kg. No significant changes were observed in any of the evaluated motor, cardiac or biochemical parameters. Conclusion: Our data suggest that intravenous administration of recombinant Phα1β may be feasible for drug-induced analgesia, without causing any severe side effects.

First record of Leptopilina boulardi Barbotin et al. (Hymenoptera: Figitidae: Eucoilinae) parasitizing of Zaprionus indianus gupta (Diptera: drosophilidae) in Brazil / Primeiro registro de Leptopilina boulardi Barbotin et al. (Hymenoptera: Figitidae: Eucoilinae) parasitando Zaprionus indianus gupta (Diptera: drosophilidae) no Brasil

This paper reports the first occurrence of the parasitoid Leptopilina boulardi Barbotin et al. (Hymenoptera: Figitidae: Eucoilinae) collected in pupae of Zaprionus indianus Gupta (Diptera: Drosophilidae) using traps baited with fruits (pear, apple and banana). The experiment was undertaken at the Faculdade de Agronomia do Instituto Luterano de Ensino Superior (experimental campus), Itumbiara, Goiás, between March and November 2001. A total of 03 specimens were obtained from 139 Z. indianus pupae. The overall prevalence of parasitism was of 2.2%.

Este trabalho relata a primeira ocorrência do parasitóide Leptopilina boulardi Barbotin et al. (Hymenoptera: Figitidae: Eucoilinae) em pupas de Zaprionus indianus Gupta (Diptera: Drosophilidae) obtidas usando armadilhas contendo isca á base de frutas (pêra, maçã e banana). O experimento foi realizado na Faculdade de Agronomia do Instituto Luterano de Ensino Superior (campus experimental), Itumbiara, Goiás, entre março e novembro de 2001. Coletaram-se 03 espécimes de L. boulardi em 139 pupas de Z. indianus, totalizando 2,2%.

First record of Leptopilina boulardi Barbotin et al. (Hymenoptera: Figitidae: Eucoilinae) parasitizing of Zaprionus indianus gupta (Diptera: drosophilidae) in Brazil / Primeiro registro de Leptopilina boulardi Barbotin et al. (Hymenoptera: Figitidae: Eucoilinae) parasitando Zaprionus indianus gupta (Diptera: drosophilidae) no Brasil

This paper reports the first occurrence of the parasitoid Leptopilina boulardi Barbotin et al. (Hymenoptera: Figitidae: Eucoilinae) collected in pupae of Zaprionus indianus Gupta (Diptera: Drosophilidae) using traps baited with fruits (pear, apple and banana). The experiment was undertaken at the Faculdade de Agronomia do Instituto Luterano de Ensino Superior (experimental campus), Itumbiara, Goiás, between March and November 2001. A total of 03 specimens were obtained from 139 Z. indianus pupae. The overall prevalence of parasitism was of 2.2%.

Este trabalho relata a primeira ocorrência do parasitóide Leptopilina boulardi Barbotin et al. (Hymenoptera: Figitidae: Eucoilinae) em pupas de Zaprionus indianus Gupta (Diptera: Drosophilidae) obtidas usando armadilhas contendo isca á base de frutas (pêra, maçã e banana). O experimento foi realizado na Faculdade de Agronomia do Instituto Luterano de Ensino Superior (campus experimental), Itumbiara, Goiás, entre março e novembro de 2001. Coletaram-se 03 espécimes de L. boulardi em 139 pupas de Z. indianus, totalizando 2,2%.

First occurrence of parasitoid Hemencyrtus herbertii Ashmead {Hymenoptera: Encyrtidae) in pupae of Hemilucilia flavifacies Enderlein {Diptera: Calliphoridae) in Brazil

This study report first occurrence of parasitoid Hemencyrtus herbertii Ashmead (Hymenoptera: Encyrtidae) in pupae of Hemiluciliaflavifacies Enderlein (Oiptera: Calliphoridae) in Brazil .The pupae were obtained by the flotation method. They were individuallyplaced in gelatin capsules until the emergence of the adult flies or their parasitoids. The overall prevalence of parasitism was 19,8%.

First occurrence of parasitoid Hemencyrtus herbertii Ashmead {Hymenoptera: Encyrtidae) in pupae of Hemilucilia flavifacies Enderlein {Diptera: Calliphoridae) in Brazil

This study report first occurrence of parasitoid Hemencyrtus herbertii Ashmead (Hymenoptera: Encyrtidae) in pupae of Hemiluciliaflavifacies Enderlein (Oiptera: Calliphoridae) in Brazil .The pupae were obtained by the flotation method. They were individuallyplaced in gelatin capsules until the emergence of the adult flies or their parasitoids. The overall prevalence of parasitism was 19,8%.