Indução do estro em cutias (Dasyprocta leporina) utilizando-se protocolos à base de prostaglandina isolada ou em associação com análogo de GnRH / Estrus induction in agoutis (Dasyprocta leporina) using protocols based on prostaglandin isolated or in association to GnRH analogue

Comparou-se a eficiência de protocolos para indução de estro em cutias. Em cinco fêmeas, foram administradas duas doses de cloprostenol (5µg) com intervalo de nove dias, via intraperitoneal; em outras cinco, administraram-se 30µg de análogo do hormônio liberador de gonadotrofinas (GnRH), via intravulvar, seguidos de 5µg de cloprostenol, via intraperitoneal, após sete dias e, após mais dois dias, nova dose do análogo de GnRH. A cada três dias, a ciclicidade reprodutiva dos animais foi monitorada, por meio de coleta de sangue, para dosagem hormonal, ultrassonografia ovariana e citologia vaginal. Duas das fêmeas que receberam apenas prostaglandina, as quais estavam em fase luteal no início do tratamento, manifestaram o estro aos três e seis dias após a segunda administração da droga. Já nas fêmeas que receberam a prostaglandina associada ao análogo do GnRH, duas que originalmente estavam em fase luteal apresentaram estro aos quatro dias após o tratamento, e uma outra apenas após 10 dias. Não foram evidenciadas diferenças estatísticas quanto à eficiência dos tratamentos (P>0,05). Conclui-se que, de acordo com os protocolos utilizados, o uso da prostaglandina isolada ou em associação com análogo do GnRH para a indução do estro em cutias D. leporina apresenta eficiência limitada às fêmeas que estejam em fase luteal por ocasião do início do tratamento.(AU)

We compared the efficiency of protocols for estrus induction in agoutis. Five females received double intraperitoneal administration of cloprostenol (5µg) on a 2-days interval; other five females were treated with intravulvar administration of 30µg gonadotrophin release hormone analogue (GnRH associated to intraperitoneal administration of 5µg cloprostenol after seven days and a new administration of GnRH analogue after two days. Every 3 days, the agoutis' reproductive cycle was monitored by blood collection for hormonal analysis, ovarian ultrasound and vaginal cytology. Two females, originally in luteal phase, that received isolated prostaglandin presented estrous signs at 3 and 6 days after the second drug administration. From the females that received the association, two that were originally in luteal phase presented estrus at 4 days after treatment, and one other presented estrus only after 10 days. There was no significant statistical difference regarding the efficiency of treatments for estrus induction (P>0.05). We conclude that, according to the protocols tested in the study, the use of isolated prostaglandin or its association to GnRH analogue for estrus induction in D. leporine shows an efficiency limited to the females that were in luteal phase in the beginning of the treatment.(AU)

Indução do estro em cutias (Dasyprocta leporina) utilizando-se protocolos à base de prostaglandina isolada ou em associação com análogo de GnRH / Estrus induction in agoutis (Dasyprocta leporina) using protocols based on prostaglandin isolated or in association to GnRH analogue

Comparou-se a eficiência de protocolos para indução de estro em cutias. Em cinco fêmeas, foram administradas duas doses de cloprostenol (5µg) com intervalo de nove dias, via intraperitoneal; em outras cinco, administraram-se 30µg de análogo do hormônio liberador de gonadotrofinas (GnRH), via intravulvar, seguidos de 5µg de cloprostenol, via intraperitoneal, após sete dias e, após mais dois dias, nova dose do análogo de GnRH. A cada três dias, a ciclicidade reprodutiva dos animais foi monitorada, por meio de coleta de sangue, para dosagem hormonal, ultrassonografia ovariana e citologia vaginal. Duas das fêmeas que receberam apenas prostaglandina, as quais estavam em fase luteal no início do tratamento, manifestaram o estro aos três e seis dias após a segunda administração da droga. Já nas fêmeas que receberam a prostaglandina associada ao análogo do GnRH, duas que originalmente estavam em fase luteal apresentaram estro aos quatro dias após o tratamento, e uma outra apenas após 10 dias. Não foram evidenciadas diferenças estatísticas quanto à eficiência dos tratamentos (P>0,05). Conclui-se que, de acordo com os protocolos utilizados, o uso da prostaglandina isolada ou em associação com análogo do GnRH para a indução do estro em cutias D. leporina apresenta eficiência limitada às fêmeas que estejam em fase luteal por ocasião do início do tratamento.(AU)

We compared the efficiency of protocols for estrus induction in agoutis. Five females received double intraperitoneal administration of cloprostenol (5µg) on a 2-days interval; other five females were treated with intravulvar administration of 30µg gonadotrophin release hormone analogue (GnRH associated to intraperitoneal administration of 5µg cloprostenol after seven days and a new administration of GnRH analogue after two days. Every 3 days, the agoutis' reproductive cycle was monitored by blood collection for hormonal analysis, ovarian ultrasound and vaginal cytology. Two females, originally in luteal phase, that received isolated prostaglandin presented estrous signs at 3 and 6 days after the second drug administration. From the females that received the association, two that were originally in luteal phase presented estrus at 4 days after treatment, and one other presented estrus only after 10 days. There was no significant statistical difference regarding the efficiency of treatments for estrus induction (P>0.05). We conclude that, according to the protocols tested in the study, the use of isolated prostaglandin or its association to GnRH analogue for estrus induction in D. leporine shows an efficiency limited to the females that were in luteal phase in the beginning of the treatment.(AU)

In ovo supplementation of 25(OH)D3 to broiler embryos

A dose of 0.3 mL of water solution containing 0.00 (control), 0.625, 1.250 or 1.875 µg of 25-hydroxy cholecalciferol (25(OH)D3) was administered to 312 fertile eggs derived from 49-w-old Cobb 500 broiler breeders on the 17th day of incubation (DE17) via allantoic cavity. After treatment, eggs were distributed and maintained until hatching in four incubators set at 37.8 ºC and 55% RH. Each incubator received eggs from all treatments, according to a block design with four treatments of 77-79 replicates each. Hatching was checked every two hours from 484h to 512h of incubation to evaluate productivity and chick qualities. Chicks were housed until 10 days of age in heated battery cages according to a block design with four treatments of 10 replicates of six chicks each for performance and mortality evaluation. Mean hatching time of the chicks treated with 25(OH)D3 during the embryonic phase occurred 4 to 5 h earlier than control group (502:31h), with no effects on hatching or neonate qualities. An inverse linear effect of 25(OH)D3 dose on chick body weight at hatching was observed, but 10-d-old broiler performance and mortality were not affected. The fast body weight recovery of the broilers obtained from the embryos supplemented with the highest 25(OH)D3 level was recorded until 10 days of rearing, equaling final mean body weights (p>0.05) among experimental groups. The results of this study indicate the potential use of 25(OH)D3 as exogenous vitamin supplementation to embryos a few days before hatching without affecting neonate qualities and 10-d-old broiler chicken performance.

In ovo supplementation of 25(OH)D3 to broiler embryos

A dose of 0.3 mL of water solution containing 0.00 (control), 0.625, 1.250 or 1.875 µg of 25-hydroxy cholecalciferol (25(OH)D3) was administered to 312 fertile eggs derived from 49-w-old Cobb 500 broiler breeders on the 17th day of incubation (DE17) via allantoic cavity. After treatment, eggs were distributed and maintained until hatching in four incubators set at 37.8 ºC and 55% RH. Each incubator received eggs from all treatments, according to a block design with four treatments of 77-79 replicates each. Hatching was checked every two hours from 484h to 512h of incubation to evaluate productivity and chick qualities. Chicks were housed until 10 days of age in heated battery cages according to a block design with four treatments of 10 replicates of six chicks each for performance and mortality evaluation. Mean hatching time of the chicks treated with 25(OH)D3 during the embryonic phase occurred 4 to 5 h earlier than control group (502:31h), with no effects on hatching or neonate qualities. An inverse linear effect of 25(OH)D3 dose on chick body weight at hatching was observed, but 10-d-old broiler performance and mortality were not affected. The fast body weight recovery of the broilers obtained from the embryos supplemented with the highest 25(OH)D3 level was recorded until 10 days of rearing, equaling final mean body weights (p>0.05) among experimental groups. The results of this study indicate the potential use of 25(OH)D3 as exogenous vitamin supplementation to embryos a few days before hatching without affecting neonate qualities and 10-d-old broiler chicken performance.(AU)

Efeito do gérmen integral de milho sobre o desempenho e rendimento de carcaça de frangos de corte / Effects of corn germ meal on broiler performance and carcass yield

Realizaram-se dois experimentos com 1008 pintos machos Ag-Ross 508 em cada um deles. No primeiro, avaliou-se o desempenho de frangos de corte alimentados com diferentes níveis de gérmen integral de milho (GIM) na dieta de um a sete dias de idade (fase pré-inicial). No segundo, avaliou-se o desempenho e o rendimento de carcaça no período de oito a 47 dias. As aves foram alojadas em 16 unidades, divididas em quatro tratamentos, de acordo com os níveis de GIM, em substituição ao milho na dieta (0%, 33%, 67% e 100%), e quatro repetições de 63 aves cada. Utilizou-se o delineamento inteiramente ao acaso, e os dados foram analisados por regressão polinomial. O GIM não foi um bom alimento para a fase pré-inicial. A inclusão recomendada do GIM foi de 21,9% e 22,5% nos períodos de oito a 21 dias e de 22 a 38 dias, respectivamente. Não houve restrição do uso do GIM na fase final.(AU)

Two experiments were carried out with 1008 male Ag-Ross 508 broilers each. Experiment 1 was carried out from October 26th to November 1st, 2001 to evaluate the performance of broilers fed on increasing levels of corn germ meal (CGM) in the diets from 1 to 7 days of age. Experiment 2 was carried from January 24th to March 12th, 2002 to evaluate the performance and carcass yield from 8 to 47 days. The birds were allotted to a completely randomized design with four treatments (levels of CGM replacing corn in ration - 0%, 33%, 67% and 100%) and four replicates of 63 birds. The CGM was not a good ingredient for pre-starter phase. The recommended inclusion levels of CGM were 21.9% and 22.5% from 8 to 21 days and from 22 to 38 days, respectively. No restriction concerning the use of CGM for diets of broilers from 39 to 47 days of age is made.(AU)