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1.
Rev. bras. reprod. anim ; 47(2): 286-293, abr.-jun. 2023.
Artigo em Português | VETINDEX | ID: biblio-1435393

Resumo

A criação de cães de raça vem aumentando significativamente, consequentemente a exigência de serviços veterinários no mercado para lidar com essa categoria específica também aumentou. Em especial, temos os serviços de reprodução que crescem devido a procura por melhoria da qualidade de plantel, uma vez que pela incrementação de biotécnicas é possível dinamizar o potencial de crescimento da criação. Logo, é ideal que o profissional que irá prestar este serviço seja altamente capacitado para sua realização, tendo noções não somente de reprodução, mas também de outras necessidades que a criação de cães requer. É importante que o profissional saiba selecionar os animais adequados a reprodução, tanto reprodutores, quanto matrizes, além de quando realizar os procedimentos, assim, evitar protocolos desnecessários e que possam comprometer a criação. Desta forma, o profissional pode fornecer o serviço mais completo possível, fazendo com que esse profissional seja referência no mercado.(AU)


The breeding of purebred dogs has increased significantly, consequently the demand for veterinary services on the market to deal with this specific category has also increased. In particular, we have reproduction services that grow due to the demand for improving the quality of the herd, since by increasing biotechniques it is possible to boost the growth potential of creation. Therefore, it is ideal that the professional who will provide this service is highly qualified for its performance, having notions not only of reproduction, but also of other needs that dog breeding requires. It is important that the professional knows how to select the appropriate animals for reproduction, both breeders and matrices, in addition to when to perform the procedures, thus avoiding unnecessary protocols that could compromise the creation. In this way, the professional can provide the most complete service possible, making this professional a reference on the market.(AU)


Assuntos
Animais , Cães , Médicos Veterinários , Neonatologia/métodos , Obstetrícia/métodos
2.
Rev. bras. reprod. anim ; 47(2): 294-299, abr.-jun. 2023. tab
Artigo em Português | VETINDEX | ID: biblio-1435412

Resumo

Poucas informações a respeito da biologia dos procionídeos estão disponíveis para quem busca conhecer mais sobre essas espécies. Entretanto, alguns estudos a respeito da reprodução destes animais foram realizados. As principais espécies encontradas no Brasil são o quati, o guaxinim e o jupará, e, dentre esses, o quati se destaca por ser o que possui o maior número de informações reprodutivas. A maioria dos procionídeos aparece como espécies não ameaçadas, porém a falta de informações sobre estes animais pode fazer com que estes dados possam estar defasados. É importante o conhecimento reprodutivo dos procionídeos pois, devido as ações antrópicas que ocasionam a perda de habitat natural, essas espécies, em breve, podem entrar para a lista de animais ameaçados de extinção. Assim, esse artigo tem como objetivo descrever alguns dados por meios de estudos reprodutivos que podem auxiliar ao desenvolvimento de estratégias de conservação dessas espécies.(AU)


Little information about the biology of procyonids is available for those who want to know more about these species. However, some studies regarding the reproduction of these animals were developed. The main species found in Brazil are coati, raccoon, and kinkajou, among which the coati stands out for being the one with the highest number of reproductive information. Most procyonids appear as non-threatened species, but the lack of information about these animals may cause these data to be outdated. Reproductive knowledge of procyonids is important because, due to anthropic actions that cause loss of natural habitat, these species may soon enter on the list of endangered animals. Thus, this article aims to describe some data by means of reproductive studies that can help to develop conservation strategies for these species.(AU)


Assuntos
Animais , Comportamento Sexual Animal , Procyonidae/fisiologia , Brasil
3.
Anim. Reprod. (Online) ; 19(3): e20210069, set. 2022. tab
Artigo em Inglês | VETINDEX | ID: biblio-1393202

Resumo

Morphological sperm evaluation supported by the morphometry can be used in the determination of the seminal quality and in the investigation of potential extenders. Although there are studies comparing TRIS and ACP extenders, there are no comparative studies between them for the computerized assisted semen analysis (CASA), sperm viability, membrane functionality and sperm morphometry parameters of cryopreserved canine semen. Hence, we aimed to evaluate the effects of ACP-106c and TRIS on post-freezing canine sperm quality. Five dogs were submitted to semen collection twice with one-week interval. The semen was evaluated within the parameters: total motility, vigor, concentration, viability, plasma membrane functionality, morphology and morphometry. In the morphometric evaluation, the morphologically normal sperm was measured as: length, width, area and perimeter of the head and the midpiece, tail length and total length. The parameters of ellipticity, elongation, regularity and roughness were determined. Then, the semen was divided into two aliquots that were diluted in TRIS or ACP-106c, with the addition of egg yolk and glycerol. The diluted semen was refrigerated and frozen. The thawed samples were evaluated. Total motility, viability, sperm membrane functionality and normal morphology reduced after thawing in both extenders (morphology reduced from 89.60 ± 1.3% to 84.40 ± 1.8 and 84.60 ± 1.1% in TRIS and ACP-106c, respectively). However, it did not differ between TRIS and ACP-106c. In the ACP106c the sperm head defects in cryopreserved semen were higher compared to fresh semen (P < 0.05). For all the morphometric parameters evaluated, there were no differences between fresh and cryopreserved samples (3.70 ± 0.4% vs. 2.30 ± 0.5%). In kinetics, with an interval of one week statistical differences between the extenders were found only in the parameters ALH and LIN (P < 0.05). Regardless of the extender, there were no changes in the morphometric parameters of sperm after thawing.(AU)


Assuntos
Animais , Masculino , Cães , Processamento de Imagem Assistida por Computador , Criopreservação/veterinária , Análise do Sêmen/veterinária , Sobrevivência Celular
4.
Semina ciênc. agrar ; 42(6): 3235-3246, nov.-dez. 2021. graf, tab
Artigo em Inglês | VETINDEX | ID: biblio-1370476

Resumo

The quality of post-thawing goat sperm is critical to the success of artificial insemination protocols and may be influenced by extenders, cryoprotectants, and antioxidant substances. Therefore, the objective of this study was to evaluate the effects of the antioxidant anethole on goat sperm diluted in preservation medium based on powdered coconut water (ACP-101c) and frozen. For that, each ejaculate was submitted to the following treatments: ACP-101c (control); control plus supplementation with 30, 300, or 2000 µg/ mL anethole. The samples were thawed and evaluated for morphology, kinetics, membrane integrity, and reactive oxygen species (ROS). The addition of anethole increased morphological abnormalities (P < 0.05), however, it did not affect sperm kinetics. Flow cytometry analysis showed that sperm cells cryopreserved with 300 µg/mL anethole had lower acrosome integrity than those cryopreserved in other treatments. Evaluation of oxidative stress revealed that cells stored in the presence of 2000 µg/mL anethole had small amounts of ROS when compared to those preserved in the control medium alone or supplemented with 300 µg/mL anethole (P < 0.05). After cryopreservation of sperm with 2000 µg/mL anethole, the highest percentage of viable sperm without ROS was observed (P < 0.05). In conclusion, despite reducing ROS levels, the supplementation of anethole in ACP-101c did not affect sperm kinetics or membrane integrity post-thawing, however, it did cause morphological damage to sperm.(AU)


A qualidade do espermatozoide caprino pós-descongelação é crítica para o sucesso dos protocolos de inseminação artificial e pode ser influenciada por extensores, crioprotetores e substâncias antioxidantes. Portanto, o objetivo deste estudo foi avaliar os efeitos do antioxidante anetole sobre espermatozoides caprinos diluídos em meio de conservação à base de água de coco em pó (ACP-101c) e congelados. Para tanto, cada ejaculado foi submetido aos seguintes tratamentos: ACP-101c (controle); controle mais suplementação com 30, 300 ou 2000 µg / mL de anetole. As amostras foram descongeladas e avaliadas quanto à morfologia, cinética, integridade de membranas e espécies reativas de oxigênio. A adição de anetole aumentou as anormalidades morfológicas (P < 0,05), no entanto, não afetou a cinética dos espermatozoides. A análise da citometria de fluxo mostrou que as células de esperma criopreservadas com 300 µg / mL anethole tinham integridade acrosma menor do que aquelas criopreservadas em outros tratamentos. A avaliação do estresse oxidativo revelou que as células armazenadas na presença de 2000 µg / mL anethole apresentaram pequenas quantidades de ROS quando comparadas às preservadas em meio de controle isoladamente ou suplementadas com 300 µg / mL anethole (P < 0,05). Após a criopreservação de espermatozoides com 2000 µg / mL anethole, observou-se a maior porcentagem de espermatozoides viáveis sem ROS (P < 0,05). A população com espermatozoides viáveis sem ROS foi maior quando utilizado 2.000 µg / mL (P < 0,05). Em conclusão, apesar de reduzir os níveis de ROS, a suplementação de anetole em ACP-101c não afetou a cinética espermática e a integridade da membrana pós-descongelação, entretanto, causou danos morfológicos nos espermatozoides.(AU)


Assuntos
Animais , Masculino , Sêmen , Cabras , Criopreservação , Estresse Oxidativo , Antioxidantes
5.
Acta sci. vet. (Impr.) ; 49: Pub. 1820, 2021. tab
Artigo em Português | LILACS, VETINDEX | ID: biblio-1363850

Resumo

Sperm sexing aims to separate sperm populations in carriers of the "X" or "Y" chromosome. Currently, flow cytometry is a technique that allows greater accuracy; however, it causes structural changes in sperm, reduces viability, and has a high cost. As a result, other methods have been researched, including immunosexing, which uses monoclonal antibodies to detect sex-specific surface antigens. Thus, the objective of this study was to evaluate the immunosexing technique using a monoclonal antibody against sex-specific protein (HY) in the conservation of ram and goat semen in ACP101/102c. Ejaculates from five rams and five goats were collected with the aid of an artificial vagina; they were evaluated and submitted to the immunosexing protocol, according to the manufacturer's recommendations, using the Monoclonal Antibody Kit specific for mammalian sperm with "Y" chromosomes (HY; HY Biotechnology, Rio de Janeiro, RJ, Brazil). After sexing, the supernatant was resuspended in the cryopreservation diluent: ACP ram (ACP101/102c + 20% egg yolk + 7% glycerol) and ACP goat (ACP101/102c + 2.5% egg yolk + 7% glycerol), packaged in 0.25 mL straws, refrigerated at 4°C, stabilized for 30 min, frozen in liquid nitrogen vapor (-60°C) for 15 min, immersed in liquid nitrogen, and stored in cryogenic cylinders. The samples were evaluated in natura (T1), after immunosexing (T2) and after thawing (T3) for sperm motility subjectively using conventional microscopy (40x). Plasma membrane integrity (IMP) and sperm cell morphology were evaluated by the smear staining technique using eosin-nigrosine dye, and the percentages of healthy and morphologically defect spermatozoa were determined. In the evaluation of ram semen regarding sperm motility and IMP, no statistically significant differences were observed between treatments after sexing in the evaluation of absolute data (P > 0.05), with the difference being observed only between T1 and T2, and T3 (P < 0.05). Regarding the relative percentage and sperm morphology, no statistically significant differences were observed (P > 0.05). Regarding the evaluation of goat semen samples, the motility parameters were consistent with the technique submitted; however, the IMP data did not appear as expected, requiring further evaluation for a better assessment of the technique for this species. The data obtained from ram semen submitted to the immunosexing protocol, regarding the absolute evaluation of motility and IMP, demonstrated that the non-sexed semen (T1) was superior to the sexed treatments (T2 and T3); however, it is noteworthy that freezing started with approximately 50% of the cells, since the immunosexing technique results in a loss of viability of approximately 50% of the sperm, which corresponds to the ratio of sperm carrying the X chromosome. In addition, when the data in this study were transformed into relative values, no statistical differences were observed, indicating that the immunosexing protocol, as well as the freezing protocol, did not significantly affect the quality of ram sperm cells. In relation to the immunosexing of goat semen, future studies should be conducted in vitro to define a more appropriate protocol for the species and, in addition, in vivo studies should be performed to prove the quality of the technique. It was concluded that the immunosexing process using a monoclonal antibody against sex-specific protein (HY) associated with the use of powdered coconut water diluent (ACP101/102c) in the cryopreservation of semen proved to be efficient in the in vitro evaluation of ovine species.(AU)


Assuntos
Animais , Masculino , Sêmen , Análise para Determinação do Sexo/métodos , Análise para Determinação do Sexo/veterinária , Ruminantes , Ovinos , Criopreservação/tendências , Técnicas In Vitro
6.
Acta sci. vet. (Impr.) ; 48: Pub.1740-Jan. 30, 2020. ilus, tab
Artigo em Português | VETINDEX | ID: biblio-1458263

Resumo

Background: Sperm sexing is increasing in use because pre-determining the sex of the calf allows greater profitability and promotes significant gains in the productive systems that utilize the technique. Deployment of a low-cost and practical preservation methodol-ogy may further favor the cost-benefit ratio. Flow cytometry, the most commonly used sexing technique, has high costs and is very restricted. As an alternative, immunosexing has been studied, which uses sex-specific monoclonal antibodies. Thus, the objective of this study was to evaluate the immunosexing technique in conjunction with cryopreservation in ACP-102c and examine its economic aspects with regard to ram semen.Materials, Methods & Results: Ejaculates from two ram individuals were collected with the aid of an artificial vagina, evaluated, and submitted to the immunosexing protocol, according to the manufacturer’s recommendations, using the Monoclonal Antibody Kit specific for mammalian sperm with “Y” chromosomes (HY; HY Biotechnology, Rio de Janeiro-RJ, Brazil). After sexing, the supernatant was resuspended in the cryopreservation diluent: ACP (ACP-102c + 20% egg yolk + 7% glycerol), packaged in 0.25 mL straws, refrigerated to 4°C, stabilized for 30 min, frozen in liquid nitrogen vapors (-60°C) for 15 min, immersed in liquid nitrogen, and stored in cryogenic cylinders. The samples were thawed and evaluated for sperm kinetics both by using computerized semen analysis with SCA® software (Sperm Class Analyzer version 5.0) and subjectively comparing specimens from the two animals using conventional microscopy (40x). Plasma membrane integrity (IMP) and sperm cell morphology were evaluated by the smear staining technique...


Assuntos
Animais , Alimentos de Coco , Criopreservação/veterinária , Espermatozoides , Ovinos , Preservação do Sêmen/economia , Preservação do Sêmen/veterinária , Cocos , Custos e Análise de Custo
7.
Acta sci. vet. (Impr.) ; 47: Pub.1715-2019. tab
Artigo em Português | VETINDEX | ID: biblio-1458113

Resumo

Background: Semen extenders are required to protect and preserve semen, and the development of suitable extenders iskey for artificial insemination. Although the use of Tris-based diluent is widespread, new diluents such as powdered coconut water have been developed for better sperm protection. One way to evaluate the effectiveness of diluents is throughmicroscopic analyses that evaluate sperm motility, vigor, and concentration. However, these analyses are limited, and maynot provide accurate results. New evaluation techniques have been studied, and one of the tests that can be used to addreliability to these analyses is mitochondrial activity evaluation, which can sum all the parameters, and provide a moreaccurate evaluation. Thus, the present study aimed to evaluate the efficacy of ACP-102c in cryopreserved ram semen.Materials, Methods & Results: Five semen samples were collected from two ram breeders using artificial vagina (n = 10).Each ejaculate was divided into the following two treatments: T1 - ACP-102c + 20% egg yolk + 7% glycerol and T2 - TRIS+ 20% egg yolk + 7% glycerol. Extended semen samples were then packed in 0.5 mL plastic straws, subjected through therefrigeration curve up to 4°C (0.35°C/min), and equilibrated for 2 h at 4°C. Subsequently, the straws were placed at 4 cmabove liquid nitrogen level (-60°C) for 15 min, immersed, and then finally stored in the liquid nitrogen at -196°C. Bothfresh and thawed samples were evaluated for total and progressive sperm motility using conventional microscopy (40x),and the same evaluator on each occasion. For plasma membrane integrity (IMP), the smear staining technique with theEosin-Nigrosin staining was used; 200 sperms were counted and classified as whole (unstained) and unhealthy (stained).Mitochondrial activity was evaluated using a cytochemical technique based on the oxidation of 3,3’-diaminobenzidine(DAB); 200 sperms were counted, and classified into four...


Assuntos
Masculino , Animais , Alimentos de Coco , Criopreservação/métodos , Criopreservação/veterinária , Mitocôndrias , Ovinos , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária
8.
Acta sci. vet. (Online) ; 47: Pub. 1715, Dec. 14, 2019. tab
Artigo em Português | VETINDEX | ID: vti-25447

Resumo

Background: Semen extenders are required to protect and preserve semen, and the development of suitable extenders iskey for artificial insemination. Although the use of Tris-based diluent is widespread, new diluents such as powdered coconut water have been developed for better sperm protection. One way to evaluate the effectiveness of diluents is throughmicroscopic analyses that evaluate sperm motility, vigor, and concentration. However, these analyses are limited, and maynot provide accurate results. New evaluation techniques have been studied, and one of the tests that can be used to addreliability to these analyses is mitochondrial activity evaluation, which can sum all the parameters, and provide a moreaccurate evaluation. Thus, the present study aimed to evaluate the efficacy of ACP-102c in cryopreserved ram semen.Materials, Methods & Results: Five semen samples were collected from two ram breeders using artificial vagina (n = 10).Each ejaculate was divided into the following two treatments: T1 - ACP-102c + 20% egg yolk + 7% glycerol and T2 - TRIS+ 20% egg yolk + 7% glycerol. Extended semen samples were then packed in 0.5 mL plastic straws, subjected through therefrigeration curve up to 4°C (0.35°C/min), and equilibrated for 2 h at 4°C. Subsequently, the straws were placed at 4 cmabove liquid nitrogen level (-60°C) for 15 min, immersed, and then finally stored in the liquid nitrogen at -196°C. Bothfresh and thawed samples were evaluated for total and progressive sperm motility using conventional microscopy (40x),and the same evaluator on each occasion. For plasma membrane integrity (IMP), the smear staining technique with theEosin-Nigrosin staining was used; 200 sperms were counted and classified as whole (unstained) and unhealthy (stained).Mitochondrial activity was evaluated using a cytochemical technique based on the oxidation of 3,3-diaminobenzidine(DAB); 200 sperms were counted, and classified into four...(AU)


Assuntos
Animais , Masculino , Ovinos , Criopreservação/métodos , Criopreservação/veterinária , Alimentos de Coco , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Mitocôndrias , 3,3'-Diaminobenzidina
9.
Acta sci. vet. (Impr.) ; 47: Pub.1712-2019. ilus, tab
Artigo em Inglês | VETINDEX | ID: biblio-1458110

Resumo

Background: Gray brocket deer (Mazama gouazoubira) populations have been declining due to human intervention.Yet, only a few studies have assessed ultrasonographic testicular characteristics in cervids. Considering the relevance ofmonitoring testicular size, blood flow, and parenchyma, the present study aims to establish baseline information on scrotalcircumference, testicular volume, and spectral Doppler parameters, to describe differences among adult male gray brocketdeer in different reproductive status, and to correlate ultrasound parameters with testes size measurements.Materials, Methods & Results: Six adult male gray brocket deers were used in the study. Scrotal circumference and testicularvolume were measured. B mode ultrasound images of testes (longitudinal and cross-sectional views) and epididymes weresubjected to computer-assisted analysis, obtaining the numerical pixel values (NPV) and pixel standard deviation (PSD).Using spectral Doppler, supratesticular artery blood flow velocities (peak systolic velocity - PSV, end diastolic velocity -EDV, time-average maximum velocity - TAMAX, resistivity - RI and pulsatility indices - PI) were obtained. Semen wasanalyzed through total motility, vigor, and concentration tests. Three animals were normospermic (F+ group) and threewere oligo/azoospermic (F- group). Groups were compared using were compared using a one-way ANOVA or KruskalWallis followed by Student-Newman-Keuls (SNK) test. Ultrasound parameters were correlated to testes size parametersusing Pearson’s correlation for parametric variables and Spearman’s correlation for non-parametric variables. F+ grouppresented significantly higher scrotal circumference (14.57 ± 1.19 cm), testicular volume (26.18 ± 4.94 cm3), and testescross-sectional NPV (69.88 ± 24.00) and PSD (10.78 ± 3.42) than group F- (NPV: 28.26 ± 13.75, PSD: 6.70 ± 1.84). Nosignificant differences were observed between the groups regarding...


Assuntos
Masculino , Animais , Cervos/anatomia & histologia , Escroto/anatomia & histologia , Testículo/anatomia & histologia , Testículo/diagnóstico por imagem , Ultrassonografia Doppler/veterinária , Ultrassonografia/veterinária
10.
Acta sci. vet. (Online) ; 47: Pub. 1712, Dec. 12, 2019. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-25473

Resumo

Background: Gray brocket deer (Mazama gouazoubira) populations have been declining due to human intervention.Yet, only a few studies have assessed ultrasonographic testicular characteristics in cervids. Considering the relevance ofmonitoring testicular size, blood flow, and parenchyma, the present study aims to establish baseline information on scrotalcircumference, testicular volume, and spectral Doppler parameters, to describe differences among adult male gray brocketdeer in different reproductive status, and to correlate ultrasound parameters with testes size measurements.Materials, Methods & Results: Six adult male gray brocket deers were used in the study. Scrotal circumference and testicularvolume were measured. B mode ultrasound images of testes (longitudinal and cross-sectional views) and epididymes weresubjected to computer-assisted analysis, obtaining the numerical pixel values (NPV) and pixel standard deviation (PSD).Using spectral Doppler, supratesticular artery blood flow velocities (peak systolic velocity - PSV, end diastolic velocity -EDV, time-average maximum velocity - TAMAX, resistivity - RI and pulsatility indices - PI) were obtained. Semen wasanalyzed through total motility, vigor, and concentration tests. Three animals were normospermic (F+ group) and threewere oligo/azoospermic (F- group). Groups were compared using were compared using a one-way ANOVA or KruskalWallis followed by Student-Newman-Keuls (SNK) test. Ultrasound parameters were correlated to testes size parametersusing Pearsons correlation for parametric variables and Spearmans correlation for non-parametric variables. F+ grouppresented significantly higher scrotal circumference (14.57 ± 1.19 cm), testicular volume (26.18 ± 4.94 cm3), and testescross-sectional NPV (69.88 ± 24.00) and PSD (10.78 ± 3.42) than group F- (NPV: 28.26 ± 13.75, PSD: 6.70 ± 1.84). Nosignificant differences were observed between the groups regarding...(AU)


Assuntos
Animais , Masculino , Cervos/anatomia & histologia , Testículo/anatomia & histologia , Testículo/diagnóstico por imagem , Escroto/anatomia & histologia , Ultrassonografia/veterinária , Ultrassonografia Doppler/veterinária
11.
Ciênc. Anim. (Impr.) ; 28(4): 21-24, 2018. tab
Artigo em Português | VETINDEX | ID: vti-21986

Resumo

The brown brocket deer is in risk of extinction in two brazilian states. Therefore, more studies on semen evaluation may be useful to improve male reproductive capacity assessment. In this sense, this study evaluated the sperm viability using eosin-nigrosin and compared the results of fresh and cooled semen samples. Although the viability decreased in cooled semen, it maintained a satisfactory result.(AU)


Assuntos
Animais , Masculino , Cervus brasilicus , Análise do Sêmen/veterinária , Análise do Sêmen/métodos , Espécies em Perigo de Extinção
12.
Ciênc. Anim. (Impr.) ; 28(4): 21-24, 2018. tab
Artigo em Português | VETINDEX | ID: biblio-1472434

Resumo

The brown brocket deer is in risk of extinction in two brazilian states. Therefore, more studies on semen evaluation may be useful to improve male reproductive capacity assessment. In this sense, this study evaluated the sperm viability using eosin-nigrosin and compared the results of fresh and cooled semen samples. Although the viability decreased in cooled semen, it maintained a satisfactory result.


Assuntos
Masculino , Animais , Análise do Sêmen/métodos , Análise do Sêmen/veterinária , Cervus brasilicus , Espécies em Perigo de Extinção
13.
R. bras. Reprod. Anim. ; 42(3-4): 96-100, jul.-dez. 2018.
Artigo em Português | VETINDEX | ID: vti-21016

Resumo

Na conservação seminal é necessária a utilização de diluentes que forneçam nutrientes e protejam as células espermáticas contra o choque térmico. Os aditivos de origem animal, como a gema de ovo e o leite, amplamente empregados na preservação do sêmen, representam um risco potencial de contaminação. Com o intuito de reduzir esses impactos quanto ao uso de substâncias de origem animal, esta revisão teve como objetivo abordar os principais pontos acerca da utilização de diluentes para congelação do sêmen de pequenos ruminantes livres de gema de ovo, um diluente constituído 100% por produtos de origem vegetal.(AU)


For semen conservation, it is necessary to use extenders that provide nutrients and protect the spermatozoa against thermal shock. Animal source additives, such as egg yolk and milk, widely used in semen preservation, represent a potential contamination risk. To reduce impacts related to the use of animal origin substances, this review aimed to address the main points about the use of extenders for small ruminant sperm freezing free of egg yolk, an extender composed by 100% vegetable origin products.(AU)


Assuntos
Animais , Masculino , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Crioprotetores/química , Gema de Ovo
14.
R. bras. Reprod. Anim. ; 42(3-4): 90-95, jul.-dez. 2018.
Artigo em Português | VETINDEX | ID: vti-21013

Resumo

A sexagem espermática é uma biotecnologia que consiste na separação de espermatozoides portadores do cromossomo “X” ou “Y”. Esta técnica tem se destacado na área de produção animal, uma vez que tem despertado um grande interesse comercial, devido à possibilidade de aumentar a rentabilidade dos empreendimentos. Assim, esta revisão teve como objetivo abordar as principais técnicas utilizadas para sexagem espermática e seus avanços em relação a aplicabilidade no sistema pecuário. Todavia, diversos aspectos relacionados à técnica de sexagem de células espermáticas ainda necessitam ser elucidados, a fim de aprimorar ou desenvolver novas tecnologias que servirão para o desenvolvimento de metodologias mais eficientes e acessíveis aos produtores.(AU)


The sperm sexing is a biotechnology that consists of the separation of spermatozoa bearing the X or Y chromosome. This technique has been outstanding in animal production, since it has aroused a great commercial interest, due to the possibility of increasing the profitability of the enterprises. Thus, this review aimed to approach the main techniques used for sperm sexing and its advances regarding applicability in the livestock system. However, several aspects related to the sexing technique of sperm cells still need to be elucidated to improve or develop new technologies that will serve to develop more efficient and accessible methodologies for producers.(AU)


Assuntos
Animais , Ruminantes/embriologia , Biotecnologia , Espermatozoides
15.
Rev. bras. reprod. anim ; 42(3-4): 90-95, jul.-dez. 2018.
Artigo em Português | VETINDEX | ID: biblio-1492518

Resumo

A sexagem espermática é uma biotecnologia que consiste na separação de espermatozoides portadores do cromossomo “X” ou “Y”. Esta técnica tem se destacado na área de produção animal, uma vez que tem despertado um grande interesse comercial, devido à possibilidade de aumentar a rentabilidade dos empreendimentos. Assim, esta revisão teve como objetivo abordar as principais técnicas utilizadas para sexagem espermática e seus avanços em relação a aplicabilidade no sistema pecuário. Todavia, diversos aspectos relacionados à técnica de sexagem de células espermáticas ainda necessitam ser elucidados, a fim de aprimorar ou desenvolver novas tecnologias que servirão para o desenvolvimento de metodologias mais eficientes e acessíveis aos produtores.


The sperm sexing is a biotechnology that consists of the separation of spermatozoa bearing the X or Y chromosome. This technique has been outstanding in animal production, since it has aroused a great commercial interest, due to the possibility of increasing the profitability of the enterprises. Thus, this review aimed to approach the main techniques used for sperm sexing and its advances regarding applicability in the livestock system. However, several aspects related to the sexing technique of sperm cells still need to be elucidated to improve or develop new technologies that will serve to develop more efficient and accessible methodologies for producers.


Assuntos
Animais , Biotecnologia , Espermatozoides , Ruminantes/embriologia
16.
Rev. bras. reprod. anim ; 42(3-4): 96-100, jul.-dez. 2018.
Artigo em Português | VETINDEX | ID: biblio-1492519

Resumo

Na conservação seminal é necessária a utilização de diluentes que forneçam nutrientes e protejam as células espermáticas contra o choque térmico. Os aditivos de origem animal, como a gema de ovo e o leite, amplamente empregados na preservação do sêmen, representam um risco potencial de contaminação. Com o intuito de reduzir esses impactos quanto ao uso de substâncias de origem animal, esta revisão teve como objetivo abordar os principais pontos acerca da utilização de diluentes para congelação do sêmen de pequenos ruminantes livres de gema de ovo, um diluente constituído 100% por produtos de origem vegetal.


For semen conservation, it is necessary to use extenders that provide nutrients and protect the spermatozoa against thermal shock. Animal source additives, such as egg yolk and milk, widely used in semen preservation, represent a potential contamination risk. To reduce impacts related to the use of animal origin substances, this review aimed to address the main points about the use of extenders for small ruminant sperm freezing free of egg yolk, an extender composed by 100% vegetable origin products.


Assuntos
Masculino , Animais , Crioprotetores/química , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Gema de Ovo
17.
R. bras. Reprod. Anim. ; 40(4): 439-440, Out-Dez. 2016. tab
Artigo em Português | VETINDEX | ID: vti-24283

Resumo

tThe additives of animal origin including egg yolk, widely used for sperm preservation. In order tominimize the impact on the use of animal products, the objective of this study was to evaluate the effect oflyophilized extract of Aloe vera and ACP 102® in ram semen refrigerated at 4°C for 48h. Ejaculates from threeram were split into three aliquots and diluted in ACP®, ACP®/ALV and ALV. Motility and viability was evaluatedafter sperm dilution, 2h, 24h and 48h of storage at 4°C. Results showed that there was a reduction in total andprogressive motility of fresh as compared to other storage time. No differences were observed in viability of freshand 2h as well as there was no difference of semen at for 24h and 48h storage. In conclusion, study showed that theaddition of lyophilized Aloe vera extract was able to maintain semen viability similar to ACP.(AU)


Assuntos
Animais , Ovinos/embriologia , Preservação do Sêmen/métodos , Aloe , Liofilização/métodos , Liofilização/veterinária
18.
R. bras. Reprod. Anim. ; 40(4): 436-438, Out-Dez. 2016. tab
Artigo em Português | VETINDEX | ID: vti-24205

Resumo

The study aimed to evaluate the mitochondrial activity of cryopreserved ram sperm preserved inmedium based on powdered coconut water (ACP-102c). Five sperm collections of two rams were held. Eachejaculate was divided into two treatments: T1 (ACP-102c+egg yolk+glycerol) and T2 (TRIS+eggyolk+glycerol). After dilution, the samples were filled into 0.5 ml straws, chilled to 4 °C and stabilized for 2 h.They were then frozen and stored in liquid nitrogen at -196°C. The activity mitochondrial of treatments wereevaluated in time 0h and post-thawing. Data were expressed as mean ± standard deviation and submitted to theShapiro-Wilk normality test and subsequently suffered angular transformation into arcsine of percentages. Theresults were considered significant when P < 0.05. There was no significant difference between treatments. Itwas concluded that ACP-102c was able to maintain mitochondrial activity pot-thawing.(AU)


Assuntos
Animais , Ovinos/embriologia , Alimentos de Coco , Criopreservação/métodos , Criopreservação/veterinária
19.
Rev. bras. reprod. anim ; 40(4): 436-438, Out-Dez. 2016. tab
Artigo em Português | VETINDEX | ID: biblio-1492332

Resumo

The study aimed to evaluate the mitochondrial activity of cryopreserved ram sperm preserved inmedium based on powdered coconut water (ACP-102c). Five sperm collections of two rams were held. Eachejaculate was divided into two treatments: T1 (ACP-102c+egg yolk+glycerol) and T2 (TRIS+eggyolk+glycerol). After dilution, the samples were filled into 0.5 ml straws, chilled to 4 °C and stabilized for 2 h.They were then frozen and stored in liquid nitrogen at -196°C. The activity mitochondrial of treatments wereevaluated in time 0h and post-thawing. Data were expressed as mean ± standard deviation and submitted to theShapiro-Wilk normality test and subsequently suffered angular transformation into arcsine of percentages. Theresults were considered significant when P < 0.05. There was no significant difference between treatments. Itwas concluded that ACP-102c was able to maintain mitochondrial activity pot-thawing.


Assuntos
Animais , Alimentos de Coco , Criopreservação/métodos , Criopreservação/veterinária , Ovinos/embriologia
20.
Rev. bras. reprod. anim ; 40(4): 439-440, Out-Dez. 2016. tab
Artigo em Português | VETINDEX | ID: biblio-1492333

Resumo

tThe additives of animal origin including egg yolk, widely used for sperm preservation. In order tominimize the impact on the use of animal products, the objective of this study was to evaluate the effect oflyophilized extract of Aloe vera and ACP 102® in ram semen refrigerated at 4°C for 48h. Ejaculates from threeram were split into three aliquots and diluted in ACP®, ACP®/ALV and ALV. Motility and viability was evaluatedafter sperm dilution, 2h, 24h and 48h of storage at 4°C. Results showed that there was a reduction in total andprogressive motility of fresh as compared to other storage time. No differences were observed in viability of freshand 2h as well as there was no difference of semen at for 24h and 48h storage. In conclusion, study showed that theaddition of lyophilized Aloe vera extract was able to maintain semen viability similar to ACP.


Assuntos
Animais , Aloe , Ovinos/embriologia , Preservação do Sêmen/métodos , Liofilização/métodos , Liofilização/veterinária
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