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1.
Ci. Rural ; 33(1)2003.
Artigo em Inglês | VETINDEX | ID: vti-704119

Resumo

This study aimed at evaluating the use of scanning electron microscopy in the study of the post-vaccinal respiratory reaction of the tracheal epithelium of quails (Coturnix coturnix japonica) immunized against Newcastle disease. A number of 36 quails were distributed into four groups: T1 FONT FACE=Symbol>¾ /FONT> control birds (non-vaccinated); T2 FONT FACE=Symbol>¾ /FONT> birds vaccinated with Ulster 2C strain; t3 FONT FACE=Symbol>¾ /FONT> birds vaccinated with B1 strain; t4 FONT FACE=Symbol>¾ /FONT> birds vaccinated with LaSota strain. Regardless the experimental group, birds did not show detectable clinical signs of post-vaccinal respiratory reaction. However, the analysis of tracheal fragments by scanning electron microscopy showed that birds vaccinated with B1 and LaSota strains developed epithelial sloughing of the trachea, whereas those vaccinated Ulster 2C strain did not develop this change, demonstrating intact tracheal epithelium, similar to the control group.


Este experimento foi realizado para avaliar o emprego da microscopia eletrônica de varredura no estudo da reação respiratória pós-vacinal em epitélio traqueal de codornas (Coturnix coturnix japonica) imunizadas contra a doença de Newcastle. Foram utilizadas 36 codornas que foram distribuídas em quatro grupos, sendo: T1 FONT FACE=Symbol>¾ /FONT> grupo de aves controle (não vacinado), T2 FONT FACE=Symbol>¾ /FONT> grupo de aves vacinadas com a estirpe Ulster 2C, T3 FONT FACE=Symbol>¾ /FONT> grupo vacinado com a estirpe B1, T4 FONT FACE=Symbol>¾ /FONT> grupo de aves vacinadas com a estirpe LaSota. Independente do grupo experimental, as aves não apresentaram sinais clínicos detectáveis de reação respiratória pós-vacinal. Entretanto, na análise de fragmentos traqueais, ao microscópio eletrônico de varredura, observou-se que as codornas vacinadas com as estirpes B1 e LaSota desenvolveram descamação epitelial da traquéia, enquanto as aves vacinadas com a estirpe Ulster 2C não desenvolveram tal alteração, mostrando um epitélio traqueal íntegro, semelhante ao grupo controle.

2.
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1475891

Resumo

This study aimed at evaluating the use of scanning electron microscopy in the study of the post-vaccinal respiratory reaction of the tracheal epithelium of quails (Coturnix coturnix japonica) immunized against Newcastle disease. A number of 36 quails were distributed into four groups: T1 FONT FACE=Symbol>¾ /FONT> control birds (non-vaccinated); T2 FONT FACE=Symbol>¾ /FONT> birds vaccinated with Ulster 2C strain; t3 FONT FACE=Symbol>¾ /FONT> birds vaccinated with B1 strain; t4 FONT FACE=Symbol>¾ /FONT> birds vaccinated with LaSota strain. Regardless the experimental group, birds did not show detectable clinical signs of post-vaccinal respiratory reaction. However, the analysis of tracheal fragments by scanning electron microscopy showed that birds vaccinated with B1 and LaSota strains developed epithelial sloughing of the trachea, whereas those vaccinated Ulster 2C strain did not develop this change, demonstrating intact tracheal epithelium, similar to the control group.


Este experimento foi realizado para avaliar o emprego da microscopia eletrônica de varredura no estudo da reação respiratória pós-vacinal em epitélio traqueal de codornas (Coturnix coturnix japonica) imunizadas contra a doença de Newcastle. Foram utilizadas 36 codornas que foram distribuídas em quatro grupos, sendo: T1 FONT FACE=Symbol>¾ /FONT> grupo de aves controle (não vacinado), T2 FONT FACE=Symbol>¾ /FONT> grupo de aves vacinadas com a estirpe Ulster 2C, T3 FONT FACE=Symbol>¾ /FONT> grupo vacinado com a estirpe B1, T4 FONT FACE=Symbol>¾ /FONT> grupo de aves vacinadas com a estirpe LaSota. Independente do grupo experimental, as aves não apresentaram sinais clínicos detectáveis de reação respiratória pós-vacinal. Entretanto, na análise de fragmentos traqueais, ao microscópio eletrônico de varredura, observou-se que as codornas vacinadas com as estirpes B1 e LaSota desenvolveram descamação epitelial da traquéia, enquanto as aves vacinadas com a estirpe Ulster 2C não desenvolveram tal alteração, mostrando um epitélio traqueal íntegro, semelhante ao grupo controle.

3.
Braz. J. Vet. Pathol. ; 5(2): 47-50, 2012.
Artigo em Inglês | VETINDEX | ID: vti-689798

Resumo

 This study aimed to characterize the true epidemiological role played by the Chinese goose (Anser cygnoides) as a potential source of infection by the Newcastle disease virus (NDV). For this, Specific-Pathogen-Free chicks (SPF) were used and were housed with Chinese geese that had been inoculated with a pathogenic strain (velogenic viscerotropic, strain São João do Meriti) of NDV (DIE50=108.15/0.1 mL) pathogenic to chickens, by the ocular-nasal route. Each group was composed of 6 SPF Leghorn chicks and 3 geese. At 6 days (Group I) and 14 days (Group II) after inoculation of the Chinese geese with NDV, SPF chicks were put into direct contact with each goose group. Cloacal swabs were collected from both species (Chinese geese and SPF chicks) 6, 10 and 20 days after challenge to genome viral excretion by Reverse Transcription Polymerase Chain Reaction (RT-PCR). Chinese geese did not demonstrate any clinical signs of Newcastle disease (ND). They were refractory to the clinical disease with the NDV. However, NDV genome was detected 20 days after challenge. Therefore, NDV carrier status was demonstrated by Chinese geese. Moreover, 100% of SPF chicks housed with the infected Chinese geese had died  by 6 (Group I) and 14 days (Group II) after challenge. Thus, the transmission of the pathogenic virus from the Chinese geese to cohabiting SPF chicks was evident within 20 days of the expe

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