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1.
Acta cir. bras ; 36(9): e360908, 2021. tab
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1345028

Resumo

ABSTRACT Purpose: To describe a new anesthetic protocol medullary and nerve roots access and in Rattus norvegicus. Methods: Seventy female Wistar rats (n=70) were used. The animals were randomly divided into two laminectomy groups: cervical (n=40) and thoracic (n=30). In cervical group, a right posterior hemilaminectomy was performed to access the nerve roots. In thoracic group, a laminectomy of the eighth thoracic vertebra was accomplished. Thirty-five rats (20 cervical and 15 thoracic) were submitted to old anesthetic protocol (ketamine 70 mg/kg plus xylazine 10 mg/kg); and the 35 other animals (20 cervical and 15 thoracic) were submitted to a new anesthetic protocol (ketamine 60 mg/kg,xylazine 8 mg/kg and fentanyl 0.03 mg/kg). Results: The time to complete induction was 4.15 ±1.20 minin ketamine, xylazine and fentanyl group, and it was 4.09 ±1.47 min in the ketamine and xylazine group. There was no correlation in the time required to perform the cervical laminectomy in the old anesthetic protocol. In all groups, the animals submitted to the old anesthetic protocol had a higher level of pain on the first and third postoperative days than the animals submitted to the new anesthetic protocol. Conclusions: The new anesthetic protocol reduces the surgical time, allows better maintenance of the anesthetic plan, and brings more satisfactory postoperative recovery.


Assuntos
Animais , Ratos , Ketamina , Anestésicos , Xilazina , Ratos Wistar
2.
Acta cir. bras. ; 35(7): e202000702, 2020. tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-27573

Resumo

Purpose To evaluate the normality pattern in functional tests of peripheral nerves. Methods Sixty female and sixty male Wistar rats were submitted to vibrissae movement and nictitating reflex for facial nerve; grooming test and grasping test for brachial plexus; and walking tracking test and horizontal ladder test for lumbar plexus. The tests were performed separately, with an interval of seven days between each. Results All animals showed the best score in vibrissae movement, nictitating reflex, grooming test, and horizontal ladder test. The best score was acquired for the first time in more than 90% of animals. The mean of strength on the grasping test was 133.46±12.08g for the right and 121.74±8.73g for the left anterior paw. There was a difference between the right and left sides. There was no difference between the groups according to sex. There is no statistical difference comparing all functional indexes between sex, independent of the side analyzed. The peroneal functional index showed higher levels than the sciatic and tibial functional index on both sides and sex. Conclusions The behavioral and functional assessment of peripheral nerve regeneration are low-cost, easy to perform, and reliable tests. However, they need to be performed by experienced researchers to avoid misinterpretation.(AU)


Assuntos
Animais , Ratos , Traumatismos dos Nervos Periféricos , Traumatismos dos Nervos Periféricos/diagnóstico , Comportamento Animal , Pesquisa Comportamental/métodos , Escala de Avaliação Comportamental
3.
Acta cir. bras. ; 34(7): e201900709, 2019. ilus
Artigo em Inglês | VETINDEX | ID: vti-23578

Resumo

Purpose: To develop a new low-cost, easy-to-make and available training model using chickens intestine for infant intestinal anastomosis.Methods: Segments of chicken intestine were used to create an intestinal anastomosis simulator. We tried to perform an end-to-end, end-to-side and side-to-side anastomosis. Handsewn sutured anastomosis were performed in single layered with interrupted prolene 5-0 suture. The parameters analyzed were cost, intestines diameter and length, anastomosis patency and flow-through and leakage amount. Results: In all cases it was possible to make the anastomosis in double layered without difficulties, different from the usual ones. There was a positive patency at all anastomoses after the end of the procedure, with no need for reinterventions. Conclusion: The new training model using chickens intestine for infant intestinal anastomosis is low-cost, easy-to-make and easy available.(AU)


Assuntos
Animais , Galinhas/cirurgia , Intestinos/cirurgia , Anastomose Cirúrgica/educação , Anastomose Cirúrgica/métodos , Tutoria/métodos , Procedimentos Cirúrgicos Operatórios/educação , Procedimentos Cirúrgicos Operatórios/métodos , Pediatria/métodos
4.
Acta cir. bras. ; 34(3): e201900304, Mar. 18, 2019. tab
Artigo em Inglês | VETINDEX | ID: vti-20869

Resumo

Purpose:To evaluate if the type of electrode (needle vs. surface) affects the electromyoneurography parameters in rats.Methods:Twenty male rats were anesthetized, then compound muscle action potential were recorded using a Neuropack S1 MEB- 9400©. All animals were submitted to two electroneuromyography analysis: first with surface electrode and then by needle electrode. We evaluated the latency, amplitude, duration and area of the negative peak of the gastrocnemius and cranial tibial muscles.Results:There were no significant differences between the groups in the mean of duration, latency, amplitude or area of the negative peak in gastrocnemius and cranial tibial muscles.Conclusion:The type of electrode does not affect the electroneuromyography parameters.(AU)


Assuntos
Animais , Ratos , Eletrodos/veterinária , Monitorização Neurofisiológica/veterinária , Músculo Esquelético , Exame Neurológico/veterinária
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