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1.
Semina ciênc. agrar ; 41(06,supl. 2): 3311-3322, 2020. tab, graf
Artigo em Inglês | VETINDEX | ID: biblio-1501688

Resumo

This study examines rumen-fluid parameters and the biochemical profile of feedlot sheep supplemented with a commercial product composed of yeast culture and enzymatically hydrolyzed yeast during an abrupt change of diet. Eight sheep at 30 months of age, with an average weight of 40.2 ± 3.4 kg, were housed in individual stalls where they received the same diet twice daily, which was formulated according to the National Research Council [NRC] (2007). The animals were divided into two groups, namely, supplemented with Saccharomyces cerevisiae (SG, n = 4) and control (CG, n = 4). The experiment consisted of three periods: the first (seven days) consisted of an acclimation to the roughage-only diet; in the second period (five days), the animals of SG started to receive supplementation; lastly, in the third period (five days), all animals underwent a sudden change of diet, which was characterized by an alteration from 100% roughage to a 50:50 ratio (roughage:concentrate) without prior adaptation. Blood samples were collected to evaluate albumin, aspartate amino transferase, calcium, gamma glutamyl transferase, globulins, glucose, magnesium, potassium, sodium, total plasma proteins and urea. Rumen fluid was harvested to evaluate physical aspects. Supplementation increased the protozoa count; the rate of sedimentation and fluctuation remained within the physiological parameters; and total plasma proteins and globulin decreased. Therefore, the yeast appears to be a promising alternative for reducing the deleterious effects of an abrupt change of diet.


Este estudo avaliou os parâmetros do líquido ruminal e o perfil bioquímico de ovinos confinados suplementados com um produto comercial composto por cultura de levedura e levedura hidrolisada enzimaticamente durante mudança abrupta de dieta. Foram utilizadas oito ovelhas, com 30 meses deidade e peso médio de 40,2±3,4kg, mantidas em baias individuais e receberam a mesma dieta duas vezes ao dia, formulada de acordo com o NRC (2007). Os animais foram distribuídos em dois grupos: suplementado com Saccharomyces cerevisiae (GS, n=4) e controle (GC, n=4). O experimento foi constituído de três períodos, o primeiro (sete dias) destinado a adaptação à dieta a base exclusivamente de volumoso, o segundo período (cinco dias) onde os animais do grupo GS passaram a receber suplementação e um último período (cinco dias) onde todos os animais sofreram mudança brusca de dieta, que foi caracterizada por alteração de 100% do volumoso para uma dieta na relação de 50:50 (volumoso:concentrado), sem adaptação prévia. Foram coletadas amostras de sangue, para avaliação de albumina, AST, cálcio, GGT, globulinas, glicose, magnésio, potássio, sódio, PPT (proteínas plasmáticas totais) e ureia. E líquido ruminal para avaliação de aspectos físicos. A suplementação aumentou a contagem de protozoários, a taxa de sedimentação e flutuação manteve-se dentro do fisiológico e houve redução em PPT e globulina mostrando-se uma alternativa promissora na redução dos efeitos deletérios da troca abrupta de dieta.


Assuntos
Animais , Ovinos/metabolismo , Ovinos/sangue , Ruminação Digestiva , Saccharomyces cerevisiae/metabolismo
2.
Semina Ci. agr. ; 41(06,supl. 2): 3311-3322, 2020. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-32576

Resumo

This study examines rumen-fluid parameters and the biochemical profile of feedlot sheep supplemented with a commercial product composed of yeast culture and enzymatically hydrolyzed yeast during an abrupt change of diet. Eight sheep at 30 months of age, with an average weight of 40.2 ± 3.4 kg, were housed in individual stalls where they received the same diet twice daily, which was formulated according to the National Research Council [NRC] (2007). The animals were divided into two groups, namely, supplemented with Saccharomyces cerevisiae (SG, n = 4) and control (CG, n = 4). The experiment consisted of three periods: the first (seven days) consisted of an acclimation to the roughage-only diet; in the second period (five days), the animals of SG started to receive supplementation; lastly, in the third period (five days), all animals underwent a sudden change of diet, which was characterized by an alteration from 100% roughage to a 50:50 ratio (roughage:concentrate) without prior adaptation. Blood samples were collected to evaluate albumin, aspartate amino transferase, calcium, gamma glutamyl transferase, globulins, glucose, magnesium, potassium, sodium, total plasma proteins and urea. Rumen fluid was harvested to evaluate physical aspects. Supplementation increased the protozoa count; the rate of sedimentation and fluctuation remained within the physiological parameters; and total plasma proteins and globulin decreased. Therefore, the yeast appears to be a promising alternative for reducing the deleterious effects of an abrupt change of diet.(AU)


Este estudo avaliou os parâmetros do líquido ruminal e o perfil bioquímico de ovinos confinados suplementados com um produto comercial composto por cultura de levedura e levedura hidrolisada enzimaticamente durante mudança abrupta de dieta. Foram utilizadas oito ovelhas, com 30 meses deidade e peso médio de 40,2±3,4kg, mantidas em baias individuais e receberam a mesma dieta duas vezes ao dia, formulada de acordo com o NRC (2007). Os animais foram distribuídos em dois grupos: suplementado com Saccharomyces cerevisiae (GS, n=4) e controle (GC, n=4). O experimento foi constituído de três períodos, o primeiro (sete dias) destinado a adaptação à dieta a base exclusivamente de volumoso, o segundo período (cinco dias) onde os animais do grupo GS passaram a receber suplementação e um último período (cinco dias) onde todos os animais sofreram mudança brusca de dieta, que foi caracterizada por alteração de 100% do volumoso para uma dieta na relação de 50:50 (volumoso:concentrado), sem adaptação prévia. Foram coletadas amostras de sangue, para avaliação de albumina, AST, cálcio, GGT, globulinas, glicose, magnésio, potássio, sódio, PPT (proteínas plasmáticas totais) e ureia. E líquido ruminal para avaliação de aspectos físicos. A suplementação aumentou a contagem de protozoários, a taxa de sedimentação e flutuação manteve-se dentro do fisiológico e houve redução em PPT e globulina mostrando-se uma alternativa promissora na redução dos efeitos deletérios da troca abrupta de dieta.(AU)


Assuntos
Animais , Ovinos/sangue , Ovinos/metabolismo , Ruminação Digestiva , Saccharomyces cerevisiae/metabolismo
3.
Acta Vet. Brasilica ; 11(1): 29-34, mar. 2017. tab
Artigo em Inglês | VETINDEX | ID: biblio-1453085

Resumo

The aim of this study was to trace the sources of Salmonella contamination during the pig slaughter flowchart. Ten lots of pigs sent for slaughter were followed (four animals per lot), and two weeks before slaughter they were selected based on the presence or absence of Salmonella in their farm stalls. Stool samples were collected after stunning, and from the surface swabs of the carcass in different parts of the flowchart. Samples were also collected immediately after the animals left the dehairing machine, after opening the abdominal cavity, before the carcass entered the cooling chamber, and from jowl samples. The water samples used in the scalding tank were collected before commencing the slaughter process and after the passage of the animals. For the comparison of band patterns, the isolates were analyzed by rep-PCR. The percentage of isolation was 35.3% after stunning, 17.6% immediately after the animals left the dehairing machine, 17.6% after evisceration, 23.5% before entering the cooling chamber and 5.8% from the jowls. The serotypes obtained were: Senftenberg, Idikan, Typhimurium, Heidelberg, Minnesota, Panama and Salmonella group O:4,5. By repPCR analysis, it was found that Salmonella strains that reached the slaughterhouse in carrier pigs may not be eliminated during processing, thereby making its isolation from the carcasses possible. It was also observed that the strains introduced by animals can infect others in different stages of the slaughter flowchart, thus resulting in cross-contamination.


Assuntos
Animais , Abate de Animais , Indicadores de Contaminação , Salmonella/isolamento & purificação , Salmonelose Animal/diagnóstico , Suínos/microbiologia , Transmissão de Doença Infecciosa/veterinária
4.
Acta Vet. bras. ; 11(1): 29-34, mar. 2017. tab
Artigo em Inglês | VETINDEX | ID: vti-687026

Resumo

The aim of this study was to trace the sources of Salmonella contamination during the pig slaughter flowchart. Ten lots of pigs sent for slaughter were followed (four animals per lot), and two weeks before slaughter they were selected based on the presence or absence of Salmonella in their farm stalls. Stool samples were collected after stunning, and from the surface swabs of the carcass in different parts of the flowchart. Samples were also collected immediately after the animals left the dehairing machine, after opening the abdominal cavity, before the carcass entered the cooling chamber, and from jowl samples. The water samples used in the scalding tank were collected before commencing the slaughter process and after the passage of the animals. For the comparison of band patterns, the isolates were analyzed by rep-PCR. The percentage of isolation was 35.3% after stunning, 17.6% immediately after the animals left the dehairing machine, 17.6% after evisceration, 23.5% before entering the cooling chamber and 5.8% from the jowls. The serotypes obtained were: Senftenberg, Idikan, Typhimurium, Heidelberg, Minnesota, Panama and Salmonella group O:4,5. By repPCR analysis, it was found that Salmonella strains that reached the slaughterhouse in carrier pigs may not be eliminated during processing, thereby making its isolation from the carcasses possible. It was also observed that the strains introduced by animals can infect others in different stages of the slaughter flowchart, thus resulting in cross-contamination.(AU)


Assuntos
Animais , Suínos/microbiologia , Salmonella/isolamento & purificação , Salmonelose Animal/diagnóstico , Indicadores de Contaminação , Abate de Animais , Transmissão de Doença Infecciosa/veterinária
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