Effect of strength training versus raloxifene on bone weight, blood glucose, lipid and antioxidant profile in ovariectomized rats

We compared the effect of the treatment with strength training (ST) and raloxifene (RALOX) on bone weight, blood glucose, lipid, and antioxidant profile in ovariectomized rats. Twenty-four Wistar rats were distributed into four groups: ovariectomy + VEHICLE (control); ovariectomy + RALOX; ovariectomy + ST; ovariectomy + RALOX + ST. Thirty days after ovariectomy, the animals underwent the treatment with RALOX (750 µcg day-1) and/or ST (three sessions week-1). Thirty days after, all groups were scarified, tibia and femur were weighed, and the blood was collected for analysis of the lipid profile, glucose, and antioxidants catalase (CAT) and glutathione (GSH). The ST group showed greater femur weight (0.82 ± 0.18 g) and RALOX + ST had greater tibia weight (0.61± 0.17 g) than CONTROL with femur weight of 0.65 ± 0.08 g and tibia of 0.49 ± 0.08 g with no differences between treatments (p > 0.05). ST group showed significantly higher catalase (181.7 ± 15.4 µM g-1) compared to the other groups. In contrast, the GSH value was lower in ST group (89.2 ± 8.1 µM g-1) compared to RALOX (175.9 ± 17.1 µM g-1) and RALOX + ST (162.8 ± 12.1 µM g-1), but the values of these two groups did not differ from CONTROL(115.3 ± 21.1 µM g-1). Total cholesterol did not differ between groups (p > 0.05), but exercise alone(54.3 ± 2.5 mg dL-1) or with RALOX (53.0 ± 1.5 mg dL-1) resulted in higher HDL cholesterol than CONTROL (45.5 ± 2.5 mg dL-1). Only RALOX+ST presented lower glucose (140.3 ± 9.7 mg dL-1) values than CONTROL (201.7 ± 30.6 mg dL-1). In conclusion, ST promotes similar benefits on bone and metabolic parameters compared to pharmacological treatment in ovariectomized rats.(AU)

Use of murinometrics indices and bioelectrical impedance (BIA) in the determination of experimental obesity in oophorectomized rats / Uso de índices murinométricos e impedância bioelétrica (BIA) na determinação de obesidade experimental em ratas ooforectomizadas

In this study, we tested the use of murinometric indices and bioimpedance (BIA) to determine obesity in rats. Female Wistar rats (8 weeks/130-160 g) were divided into control and oophorectomy group. The Body Mass Index (BMI) and Lee index (LI) were used as anthropometric techniques to determine obesity, and the determination of body composition by BIA, as a way to partition body weight into fat mass and lean mass components. The dissection of muscle tissues and adipose deposits was used as a direct determination of body fat content. The groups had body weight gain (p 0.05) after the trial period, with a differential gain in body fat (p 0.05) observed by the dissection of tissue in the oophorectomy group. This gain in body fat was detected more accurately by BIA, due to the greater ability of this method to distinguish lean from fat mass. BIA was able to measure the differential gain of body fat in a BMI considered as eutrophic by murinometric indices.(AU)

Neste estudo, foi testado o uso de índices murinométricos e da bioimpedância (BIA) na determinação da obesidade em ratos. Ratas Wistar (8 semanas/130-160g) foram divididas em dois grupos: controle e ooforectomia. O Índice de Massa Corporal (IMC) e o índice de Lee (IL) foram utilizados como técnicas antropométricas para a determinação da obesidade e da composição corporal por BIA, como um meio de fracionamento do peso corporal em sua massa de gordura e componentes de massa magra. A dissecação dos tecidos musculares e depósitos adiposos foi utilizada como uma forma direta de determinação do teor de gordura corporal. Os grupos tiveram ganho de peso corporal (p 0,05) após o período experimental, com o grupo ooforectomia com ganho diferencial na gordura corporal (p 0,05), observada na dissecação do tecido adiposo. Esse ganho de gordura corporal foi percebido com maior precisão pela BIA devido à maior capacidade de diferenciação da massa corporal magra e da massa de gordura no peso corporal por meio do método. A BIA foi capaz de perceber o ganho diferencial da fração de gordura corporal em um IMC proposto como eutrófico pelos índices murinométricos.(AU)