Applying assisted reproductive technology and reproductive management to reduce CO2-equivalent emission in dairy and beef cattle: a review

Methane emission from beef and dairy cattle combined contributes around 4.5-5.0% of total anthropogenic global methane. In addition to enteric methane (CH4) produced by the rumen, cattle production also contributes carbon dioxide (CO2) (feed), nitrous oxide (N2O) (feed production, manure) and other CH4 (manure) to the total greenhouse gas (GHG) budget of beef and dairy production systems. The relative contribution in standard dairy systems is typically enteric CH4 58%, feed 29% and manure 10%. Herds with low production efficiency can have an enteric CH4 contribution up to 90%. Digestibility of feed can impact CH4 emission intensity. Low fertility herds also have a greater enteric CH4 contribution. Animals with good feed conversion efficiency have a lower emission intensity of CH4/kg of meat or milk. Feed efficient heifers tend to be lean and have delayed puberty. Fertility is a major driver of profit in both beef and dairy cattle, and it is highly important to apply multi-trait selection when shifting herds towards improved efficiency and reduced CH4. Single nucleotide polymorphisms (SNPs) have been identified for feed efficiency in cattle and are used in genomic selection. SNPs can be utilized in artificial insemination and embryo transfer to increase the proportion of cattle that have the attributes of efficiency, fertility and reduced enteric CH4. Prepubertal heifers genomically selected for favourable traits can have oocytes recovered to produce IVF embryos. Reproductive technology is predicted to be increasingly adopted to reduce generation interval and accelerate the rate of genetic gain for efficiency, fertility and low CH4 in cattle. The relatively high contribution of cattle to anthropogenic global methane has focussed attention on strategies to reduce enteric CH4 without compromising efficiency and fertility. Assisted reproductive technology has an important role in achieving the goal of multiplying and distributing cattle that have good efficiency, fertility and low CH4.(AU)

Contribution of tiller age category to herbage accumulation of Marandu palisadegrass under two fertilization regimes / Contribuição da categoria de idade do perfilho para o acúmulo de forragem de capim-marandu sob dois regimes de fertilização

Based on the hypothesis that climate and fertilizers influence the proportion of tiller age categories in the canopy and, consequently, in herbage accumulation, the objective of this study was to determine the population density and the contribution of tiller age categories for herbage accumulation of Urochloa brizantha cv. Marandu during the year and in response to phosphate and nitrogen fertilization. The treatments consisted of two fertilization strategies: low and high fertilization and three tiller ages (young, mature and old), evaluated at four times of the year: winter, early and late spring, and summer. The total number of tillers and the proportion of young tillers were higher in late spring and summer. The growth and herbage accumulation rates showed a typical seasonal pattern and were higher in the canopy under high fertilization. Old tillers contributed more to control the total stem growth rate, as well as the canopy senescence rate. The highest percentage of young tillers is related to the high herbage accumulation in the Marandu palisadegrass canopy.(AU)

Levando em consideração que os fertilizantes e o clima podem influenciar na faixa etária de perfilhos de um dossel e consequentemente no acúmulo de forragem, o objetivo desse trabalho foi determinar a densidade populacional e a contruibuição das faixas etárias de perfilhos para o acúmulo de forragem Uruchloa brizanta cv Marandu durante o ano e em resposta a fertização com fosfato e nitrogênio. O tratamento foi constituido de duas estratégias de fertilização: baixa e alta fertilização e três faixas etárias de perfilhos (jovem, maduro e velho) avaliadas em quatro épocas do ano (inverno, início e fim da primavera e verão). O número total de perfilhos e a proporção de perfilhos jovens foram maiores no final da primavera e no verão. As taxas de crescimento e de acumulação de forragem mostraram um padrão sazonal típico e foram maiores no dossel com alto nivel de fertilização do que com baixo nível de fertilizante. O perfilho velho contribuiu mais para controlar as taxas de crescimento total do caule, bem como para a taxa de senescência do dossel. A maior porcentagem de perfilhos jovens está relacionada ao alto acúmulo de forragem no dossel do capimmarandu.(AU)

Foliar fertilization at the establishment of marandu, mavuno, mulato and ypyporã grasses

Foliar fertilization with macro and micro minerals for the forage grasses establishment can be a less costly alternative for the farmer, however the possible beneÀ ts of this management technique still need to be better studied. The objective with this work was to evaluate the physiological, productive and structural responses of grasses of the Urochloa genus, depending on the application or not of foliar fertilizer during the plant establishment. Four experiments were carried out, in each of which one of the following forage grasses was evaluated: Urochloa brizantha cv. Marandu, Urochloa spp. Mulatto II, Urochloa spp. Mavuno and Urochloa spp. Ipyporã. All the experiments were carried out from November 2018 to March 2019, in a completely randomized design, with four replications. The experimental area of each experiment consisted of four plots with 12.25 m², where half the area of the plots received foliar fertilizer (FH PASTAGEM FOLIAR, with 14% N; 12% P; 12% K; 0.38 % Mg; 10.2% S; 0.24% Cu; 0.18% Mn and 0.63% Zn) and the other half did not. The response variables were evaluated: spad index, leaf area index, canopy light interception, forage production and root density. In all experiments, the foliar fertilizer application during the establishment of Marandu, Mulato II, Mavuno and Ipyporã grasses did not inÁ uence (P>0,05) the spad and leaf area indexes, forage production and root density.(AU)

Diet crude protein reduction on follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows

This study evaluated the effect of crude protein (CP) reduction in four diets (156, 139, 132, and 127 g Kg-1 DM) maintaining constant metabolizable protein (188 g/day) on the follicular fluid and cumulus-oocyte complexes of mid-lactating Girolando cows. Twenty-two Girolando cows with average of 21.55 ±3.19 L daily milk yield, 105.30 ±22.62 days in lactation and 3.22 ±0.03 body condition score were selected. To reduce CP in diets and maintain constant metabolizable protein, urea and soybean meal were gradually replaced by lignosulfonate-treated soybean meal (SoyPass®, Cargill), resulting in an increase in rumen-undegradable protein and a reduction in rumen degradable protein. A linear and quadratic reduction was observed in the plasma and follicular fluid urea nitrogen concentration following CP reduction, with the most intense reduction occurring in the 127 g Kg-1 DM group (p<0.001). As CP reduced, there was a tendency for a linear increase in the follicular growth rate (P=0.0696), on the number and proportion of viable oocytes (P<0.09), and also a linear increase for the number (P=0.0397) and proportion (P<0.09) of grade I viable oocytes. Plus, there was a linear effect for the number of cumulus oophorus cells. Cows fed with the lowest amount of CP had cumulus-oocyte complexes with higher numbers of cumulus oophorus cells (P=0.0238). Also, the reduction of diet crude protein was followed by a decrease in the probability of oocytes' DNA degradation. In conclusion, the reduction of CP in the diet of mid-lactating Girolando cows, reduces urea nitrogen concentration in both blood plasma and follicular fluid, and, as a consequence, increases the viability of oocytes and the number of cumulus oophorus cells while reducing oocytes' DNA degradation of follicular included cumulus-oocyte complex. The reduction on dietary CP may improve in vivo oocytes' embryo development impacting fertility of lactating dairy cows.(AU)

Characterization of vegetative and reproductive tillers as a basis to recommend heights and nitrogen doses for the stockpillage of Marandu palisade grass

This article aims to determine the adequate pasture height and the nitrogen dose at the beginning of the deferral period for Urochloa brizantha cv. Marandu (Marandu palisade grass). Two pasture heights (15 and 30 cm) and four nitrogen doses (0, 40, 80, and 120 kg ha-1) were evaluated in a completely randomized factorial (4x2) design with three replications. A linear increase in the number of vegetative tillers was observed with the application of nitrogen in the 15-cm deferred pasture. The lengths of stem and leaf blades of tillers increased linearly with the application of nitrogen. The weight of the vegetative tillers was greater in the 30-cm deferred pasture, compared to the 15-cm one when the pasture was fertilized with 0 and 40 kg ha-1 of N. The nitrogen fertilization linearly increased the weight of both the vegetative tillers in the 15-cm deferred pastures and the reproductive tillers in the 30-cm deferred pastures. The recommendation is that Marandu palisade grass be deferred with 15 cm and fertilized with 80 kg ha-1 of N at the beginning of the deferment period.

Characterization of vegetative and reproductive tillers as a basis to recommend heights and nitrogen doses for the stockpillage of Marandu palisade grass

This article aims to determine the adequate pasture height and the nitrogen dose at the beginning of the deferral period for Urochloa brizantha cv. Marandu (Marandu palisade grass). Two pasture heights (15 and 30 cm) and four nitrogen doses (0, 40, 80, and 120 kg ha-1) were evaluated in a completely randomized factorial (4x2) design with three replications. A linear increase in the number of vegetative tillers was observed with the application of nitrogen in the 15-cm deferred pasture. The lengths of stem and leaf blades of tillers increased linearly with the application of nitrogen. The weight of the vegetative tillers was greater in the 30-cm deferred pasture, compared to the 15-cm one when the pasture was fertilized with 0 and 40 kg ha-1 of N. The nitrogen fertilization linearly increased the weight of both the vegetative tillers in the 15-cm deferred pastures and the reproductive tillers in the 30-cm deferred pastures. The recommendation is that Marandu palisade grass be deferred with 15 cm and fertilized with 80 kg ha-1 of N at the beginning of the deferment period.(AU)

Influência da progesterona injetável na taxa de prenhez e perda gestacional de fêmeas nelore de distintas categorias reprodutivas submetidas a inseminação artificial em tempo fixo / Influence of injectable progesterone on the pregnancy rate and gestational loss of Nellore females of different reproductive categories undergoing fixed-time artificial insemination

Progesterone (P4) is essential for embryonic development and maintenance of pregnancy when deficiency causes early embryonic loss. In this study, we investigated the ability of hormonal supplementation to improve the fertility of Nellore females subjected to fixed-time artificial insemination (IATF) protocols. Here, we evaluated the effect of long-acting injectable progesterone (iP4) supplementation in the D4 after IATF on pregnancy rate and pregnancy loss in Nellore females (Bos taurus indicus) from different reproductive categories in Western Amazonia. Eight hundred thirteen Nellore females from 5 farms were selected and distributed into 2 groups: control [GC; administration of 0.5 mL of 0.9% saline solution, intramuscularly - IM] (n = 407) and a group that received injectable progesterone (iP4) that was long-acting [GiP4; administration of 0.5 mL of iP4, 300 mg, via IM four days after IATF] (n = 406). Each group contained 3 subgroups: heifers, primiparous cows, and multiparous cows. Of the 407 animals in the CG, 103 were heifers, 107 primiparous, and 197 multiparous. Of the 406 animals in the GiP4 group, there were 101 heifers, 107 primiparous, and 198 multiparous. On a random day of the estrous cycle (D0), an intravaginal device containing 1 g of P4 and 2 mg of estradiol benzoate (BE) was inserted by intramuscular injection. On D8, the P4 device was removed and 150 µg of D-cloprostenol (PGF2α), 300 IU eCG, and 1 mg BE were administered IM. Cows were inseminated at D10, 48-52 h after procedure on D8. Pregnancy diagnosis was made between 35 and 40 days after insemination through ultrasound examination. Between 80 and 90 days after insemination, a new ultrasound examination was performed to assess early pregnancy loss. The data were processed using the SAS 9.2. The conception rate, pregnancy loss, and final conception rate were analyzed using PROC GLIMMIX according to groups (CG and GiP4), categories (heifers, primiparous and multiparous), and their interactions as variables. The differences in the means of least squares were adjusted using the Tukey­Kramer method. Statistical significance was defined as P < 0.05. The general conception rate was 46% (375/816). Regardless of the animal class, GiP4 animals (51.97%) had higher conception rates (P < 0.05) than CG (40.29%). In the subgroups (heifers, primiparous and multiparous cows), there was a difference (P < 0.05) between animals treated with iP4 (52.48%, 57.94%, and 48.48%, respectively) and those who were not (39.81%, 41.12%, and 40.10%, respectively). Gestational losses, regardless of the animal class, were higher in females in the CG (7.93%) [P < 0.05] compared to those in the GiP4 group (2.84%). Regardless of treatment with iP4, the percentage of gestational loss in heifers was significantly higher (10.64%) than that in primiparous and multiparous cows (3.77% and 2.86%, respectively). The final conception rates were higher in animals that received long-acting iP4, which increased the final pregnancy in all parity categories. In the present study, the use of iP4 increased the pregnancy rate in Nellore females, regardless of the category. Although there has been no consensus on the use of iP4, there is an agreement that increases in the pregnancy rate are related to the moment of exogenous P4 application. In addition to influencing the pregnancy rate, reduction in pregnancy losses is also attributed to iP4 treatment, a fact demonstrated in the present study, where animals treated with iP4 had a lower pregnancy loss rate than normally occurs in beef cattle. Supplementation with long-acting iP4 increased the pregnancy rate at D35-40, reduced pregnancy losses, and increased the conception rate on D80-90 days in Nellore females reared in the Western Amazon, regardless of the animal category.(AU)

Retorno de investimento de sistemas de tratamento de resíduos em granjas de suínos / Return on investment of wastewater treatment systems in swine farms

Os investimentos em sistemas de tratamento de resíduos de suínos (STRS) buscam muito mais contemplar questões de segurança, sanidade e de atendimento a requisitos da legislação ambiental do que investigar possibilidades de explorar economicamente os resíduos devidamente tratados. Este estudo analisou o retorno de investimentos de diferentes STRS sendo realizado entre os meses de outubro de 2013 a maio de 2014. Para a coleta de dados, foi elaborado um questionário eletrônico aplicado por meio de entrevistas em 37 granjas do estado de São Paulo escolhidas aleatoriamente. Para análise de viabilidade econômica dos diferentes STRS, utilizou-se a técnica de orçamento de capital (PAYBACK e TIR). As granjas foram divididas em quatro categorias: GEEBC - Geração de energia elétrica, biofertilizante e créditos de carbono; GEEBI - Geração de energia elétrica e biofertilizante; GEEEL - Geração de energia elétrica; SGPVA - Sem geração de produtos de valor agregado. O melhor resultado econômico foi observado na categoria GEEBC com tempo médio de retorno financeiro de nove anos. Observou-se que 73% dos pesquisados não exploravam o valor econômico dos resíduos tratados, mantendo seus investimentos com intuito de contemplar a legislação ambiental vigente.

The investments in wastewater treatment of swine systems (WTSS) search on safety, health and fulfillment of the requirements of environmental legislation to investigate to the possibilities of economically exploit properly treated effluent. This study analyzed the return on investment of different WTSS. Was being carried out between the months of October of 2013 to May of 2014. For data collection an electronic questionnaire applied through interviews on 37 farms in the State of São Paulo chosen randomly. To analyze the economic feasibility of different WTSS we used the technique of Capital Budget (PAYBACK and IRR). The farms were divided into four categories: GEEBC-generated electricity, biofertilizer and carbon credits; GEEBI-generated electricity and biofertilizer; GEEEL-generated electricity; NGVAP- no generating value-added products. The best economic result was observed in the category GEEBC with an average Payback time of nine years. It was observed that 73% of respondents do not exploit the economic value of the treated effluent, keeping your investments with a view to contemplate the environmental regulations.

Retorno de investimento de sistemas de tratamento de resíduos em granjas de suínos / Return on investment of wastewater treatment systems in swine farms

Os investimentos em sistemas de tratamento de resíduos de suínos (STRS) buscam muito mais contemplar questões de segurança, sanidade e de atendimento a requisitos da legislação ambiental do que investigar possibilidades de explorar economicamente os resíduos devidamente tratados. Este estudo analisou o retorno de investimentos de diferentes STRS sendo realizado entre os meses de outubro de 2013 a maio de 2014. Para a coleta de dados, foi elaborado um questionário eletrônico aplicado por meio de entrevistas em 37 granjas do estado de São Paulo escolhidas aleatoriamente. Para análise de viabilidade econômica dos diferentes STRS, utilizou-se a técnica de orçamento de capital (PAYBACK e TIR). As granjas foram divididas em quatro categorias: GEEBC - Geração de energia elétrica, biofertilizante e créditos de carbono; GEEBI - Geração de energia elétrica e biofertilizante; GEEEL - Geração de energia elétrica; SGPVA - Sem geração de produtos de valor agregado. O melhor resultado econômico foi observado na categoria GEEBC com tempo médio de retorno financeiro de nove anos. Observou-se que 73% dos pesquisados não exploravam o valor econômico dos resíduos tratados, mantendo seus investimentos com intuito de contemplar a legislação ambiental vigente.(AU)

The investments in wastewater treatment of swine systems (WTSS) search on safety, health and fulfillment of the requirements of environmental legislation to investigate to the possibilities of economically exploit properly treated effluent. This study analyzed the return on investment of different WTSS. Was being carried out between the months of October of 2013 to May of 2014. For data collection an electronic questionnaire applied through interviews on 37 farms in the State of São Paulo chosen randomly. To analyze the economic feasibility of different WTSS we used the technique of Capital Budget (PAYBACK and IRR). The farms were divided into four categories: GEEBC-generated electricity, biofertilizer and carbon credits; GEEBI-generated electricity and biofertilizer; GEEEL-generated electricity; NGVAP- no generating value-added products. The best economic result was observed in the category GEEBC with an average Payback time of nine years. It was observed that 73% of respondents do not exploit the economic value of the treated effluent, keeping your investments with a view to contemplate the environmental regulations.(AU)

Initial height and nitrogen fertilisation on deferred pastures of marandu palisadegrass / Altura inicial e adubação nitrogenada em pastos diferidos de capim-marandu

In deferred pastures, nitrogen fertilisation and pasture height at the beginning of the deferment period modify the structure and nutritive value of forage. This work was conducted to determine the nitrogen (N) doses and sward heights that were adequate for the deferment of Brachiaria brizantha cv. Marandu syn. Urochloa brizantha cv. Marandu (marandu palisadegrass). Two sward heights (15 and 30 cm) and four N rates (0, 40, 80,120 kg/ha) at the beginning of the deferment period were evaluated in a randomised block design and a factorial scheme with three replications. At the end of the deferment period, the numbers of live and dead tillers, the numbers of tillers with different sizes, the masses of live leaf lamina, dead leaf lamina, live stem and dead stem, the litter mass and the crude protein and neutral detergent fibre contents of the forage were evaluated. With nitrogen fertilisation, there was an increase in the masses of live leaf and live stem in the deferred canopy. With the application of 80 kg/ha of N, the litter mass was higher in the deferred canopy with a height of 30 cm, in relation to that with a height of 15 cm. The highest canopy presented greater stem mass and worse nutritional value when compared to the lowest one. In order to improve forage characteristics, marandu palisadegrass can be deferred by 15 cm and fertilised with 80 kg/ha N. When marandu palisade grass is deferred to 30 cm, nitrogen doses lower than 80 kg/ha can be applied.

Em pastagens diferidas, a adubação nitrogenada e a altura do pasto no inicio do período de diferimento modificam a estrutura e o valor nutritivo da forragem. Esse trabalho foi conduzido para determinar as doses de nitrogênio (N) e as alturas do dossel mais adequadas para o diferimento da Urochloa brizantha cv. Marandu (capim-marandu). Duas alturas do dossel (15 e 30 cm) e quatro doses de N (0, 40, 80,120 kg/ha) no inicio do período de diferimento foram avaliadas, em delineamento de blocos casualizados e esquema fatorial, com três repetições. Ao final do período de diferimento foram avaliados os números de perfilhos vivos e mortos, os números de perfilhos com diferentes tamanhos, as massas de lâmina foliar viva, lâmina foliar morta, colmo vivo e colmo morto; a massa de serrapilheira e os teores de proteína bruta e fibra em detergente neutro da forragem. Com a adubação nitrogenada, houve incremento das massas de lâmina foliar viva e de colmo vivo nos dosséis diferidos. Com aplicação de 80 kg/ha de N, a massa de serrapilheira foi maior no dossel diferido com 30 cm, em relação àquele com 15 cm. O dossel mais alto apresentou maior massa de colmo e pior valor nutritivo, se comparado ao mais baixo. Para melhorar as características da forragem, o capim-marandu pode ser diferido com 15 cm e adubado com 80 kg/ha de N. Quando o capim-marandu é diferido com 30 cm, doses de nitrogênio inferiores a 80 kg/ha podem ser aplicadas.

Initial height and nitrogen fertilisation on deferred pastures of marandu palisadegrass / Altura inicial e adubação nitrogenada em pastos diferidos de capim-marandu

In deferred pastures, nitrogen fertilisation and pasture height at the beginning of the deferment period modify the structure and nutritive value of forage. This work was conducted to determine the nitrogen (N) doses and sward heights that were adequate for the deferment of Brachiaria brizantha cv. Marandu syn. Urochloa brizantha cv. Marandu (marandu palisadegrass). Two sward heights (15 and 30 cm) and four N rates (0, 40, 80,120 kg/ha) at the beginning of the deferment period were evaluated in a randomised block design and a factorial scheme with three replications. At the end of the deferment period, the numbers of live and dead tillers, the numbers of tillers with different sizes, the masses of live leaf lamina, dead leaf lamina, live stem and dead stem, the litter mass and the crude protein and neutral detergent fibre contents of the forage were evaluated. With nitrogen fertilisation, there was an increase in the masses of live leaf and live stem in the deferred canopy. With the application of 80 kg/ha of N, the litter mass was higher in the deferred canopy with a height of 30 cm, in relation to that with a height of 15 cm. The highest canopy presented greater stem mass and worse nutritional value when compared to the lowest one. In order to improve forage characteristics, marandu palisadegrass can be deferred by 15 cm and fertilised with 80 kg/ha N. When marandu palisade grass is deferred to 30 cm, nitrogen doses lower than 80 kg/ha can be applied.(AU)

Em pastagens diferidas, a adubação nitrogenada e a altura do pasto no inicio do período de diferimento modificam a estrutura e o valor nutritivo da forragem. Esse trabalho foi conduzido para determinar as doses de nitrogênio (N) e as alturas do dossel mais adequadas para o diferimento da Urochloa brizantha cv. Marandu (capim-marandu). Duas alturas do dossel (15 e 30 cm) e quatro doses de N (0, 40, 80,120 kg/ha) no inicio do período de diferimento foram avaliadas, em delineamento de blocos casualizados e esquema fatorial, com três repetições. Ao final do período de diferimento foram avaliados os números de perfilhos vivos e mortos, os números de perfilhos com diferentes tamanhos, as massas de lâmina foliar viva, lâmina foliar morta, colmo vivo e colmo morto; a massa de serrapilheira e os teores de proteína bruta e fibra em detergente neutro da forragem. Com a adubação nitrogenada, houve incremento das massas de lâmina foliar viva e de colmo vivo nos dosséis diferidos. Com aplicação de 80 kg/ha de N, a massa de serrapilheira foi maior no dossel diferido com 30 cm, em relação àquele com 15 cm. O dossel mais alto apresentou maior massa de colmo e pior valor nutritivo, se comparado ao mais baixo. Para melhorar as características da forragem, o capim-marandu pode ser diferido com 15 cm e adubado com 80 kg/ha de N. Quando o capim-marandu é diferido com 30 cm, doses de nitrogênio inferiores a 80 kg/ha podem ser aplicadas.(AU)

Chromium supplementation modulates glucose metabolism in heatstressed Girolando dairy cows / Suplementação com cromo modula o metabolismo de glicose de vacas Girolando sob estresse térmico pelo calor

The objective of this study was to evaluate the effects of dietary chromium supplementation on hepatic gene expression of lactating Girolando (Holstein x Gyr) cows under heat stress conditions in climatic chamber. Thirty-six ¾ Holstein x Gyr lactating cows were used, based on a 2x3 factorial scheme, to evaluate the effects of two diets (0 vs 0.50 mg of organic chromium kg-¹ dry matter) and three environmental conditions (ECs): heat stress conditions in climatic chamber with ad libitum feeding (HS), a thermoneutral environment with ad libitum feeding (TN), and a pair-fed group in a thermoneutral environment (PF). Under HS group, the expression levels of glucose transporter 2(GLUT2), glucose-6-phosphatase (G6Pase), and growth hormone receptor (rGH) were down regulated (P 0.05). Heat stress caused changes in the expression of genes related to glucose metabolism, and organic chromium could modulate glucose metabolism in animals under heat stress conditions to some extent.

O objetivo deste estudo foi avaliar o efeito da suplementação com cromo na dieta na expressão gênica hepática de vacas Girolando (Holstein x Gyr) sob estresse térmico pelo calor em câmara climática. Foram utilizadas 36 vacas da raça Holandês x Gir, em um esquema fatorial 2x3, para avaliar duas dietas (0 vs 0,50 mg de cromo orgânico kg-1 de matéria seca) e três condições ambientais: condições de estresse térmico pelo calor em câmara climática com alimentação ad libitum (HS), ambiente termoneutro com alimentação ad libitum (TN) e um grupo com alimentação restrita em um ambiente termoneutro (PF). No grupo HS, as expressões de transportador de glicose 2 (GLUT2), glicose-6-fosfatase (G6Pase) e receptor de hormônio de crescimento (rGH) foram reguladas negativamente (P 0.05). O estresse térmico pelo calor causou alterações na expressão de genes relacionados ao metabolismo da glicose, e o cromo orgânico pode modular o metabolismo da glicose em animais sob essas condições de estresse pelo calor.

Structure of piatã palisadegrass deferred for two periods and fertilised with nitrogen / Estrutura do capim-piatã diferido por dois períodos e adubado com nitrogênio

The deferred pasture structure determines the consumption and performance of grazing animals and, in addition, can be influenced by the duration of the deferment period and nitrogen (N) fertilisation. The objective of this work was to evaluate the structural characteristics of Brachiaria brizantha syn. Urochloa brizantha cv. Piatã (piatã palisadegrass) deferred for two periods (79 and 127 days) and with four N doses (0, 40, 80 and 120 kg ha-1) in the Zona da Mata of Minas Gerais, Brazil. The experimental design involved complete randomised blocks and a subdivided plot scheme, with three replications. The 127-day period of deferment resulted in a higher forage mass, falling index, stem percentage and density, but a lower number of tillers, percentage and volumetric density of live leaf blade. The N dose increment, in general, worsened the structural characteristics of the piatã palisadegrass deferred for 127 days. However, with the 79-day period, the N fertilisation increased the volumetric density of live leaf blades and reduced the stem percentage in the forage mass. As a preliminary recommendation, piatã palisadegrass can be deferred for 79 days and fertilised with up to 90 kg ha-1 of N in the region of Zona da Mata de Minas Gerais, Brazil.

A estrutura do pasto diferido determina o consumo e o desempenho dos animais em pastejo e, ademais, pode ser influenciada pela duração do período de diferimento e pela adubação nitrogenada. O objetivo com este trabalho foi avaliar as características estruturais da Brachiaria brizantha syn. Urochloa brizantha cv. Piatã (capim-piatã) diferida por dois períodos (79 e 127 dias) e com quatro doses de nitrogênio (0, 40, 80 e 120 kg ha-1) na Zona da Mata de Minas Gerais, Brasil. O experimento foi em blocos completos casualizados e em esquema de parcela subdividida, com três repetições. O período de 127 dias de diferimento resultou em maiores massa de forragem, índice de tombamento, porcentagem e densidade volumétrica de colmo, porém em menores número de perfilho, porcentagem e densidade volumétrica de lâmina foliar viva. O incremento da dose de N reduziu a porcentagem de lâmina foliar viva na massa de forragem do capim-piatã diferido por 127 dias. Porém, com o período de 79 dias, a adubação aumentou a densidade volumétrica de lâmina foliar viva e reduziu a porcentagem de colmo na massa de forragem. Como recomendação preliminar, o capim-piatã pode ser diferido por 79 dias e com adubação de até 90 kg ha-1 de N na região da Zona da Mata de Minas Gerais, Brasil.

Structure of piatã palisadegrass deferred for two periods and fertilised with nitrogen / Estrutura do capim-piatã diferido por dois períodos e adubado com nitrogênio

The deferred pasture structure determines the consumption and performance of grazing animals and, in addition, can be influenced by the duration of the deferment period and nitrogen (N) fertilisation. The objective of this work was to evaluate the structural characteristics of Brachiaria brizantha syn. Urochloa brizantha cv. Piatã (piatã palisadegrass) deferred for two periods (79 and 127 days) and with four N doses (0, 40, 80 and 120 kg ha-1) in the Zona da Mata of Minas Gerais, Brazil. The experimental design involved complete randomised blocks and a subdivided plot scheme, with three replications. The 127-day period of deferment resulted in a higher forage mass, falling index, stem percentage and density, but a lower number of tillers, percentage and volumetric density of live leaf blade. The N dose increment, in general, worsened the structural characteristics of the piatã palisadegrass deferred for 127 days. However, with the 79-day period, the N fertilisation increased the volumetric density of live leaf blades and reduced the stem percentage in the forage mass. As a preliminary recommendation, piatã palisadegrass can be deferred for 79 days and fertilised with up to 90 kg ha-1 of N in the region of Zona da Mata de Minas Gerais, Brazil.(AU)

A estrutura do pasto diferido determina o consumo e o desempenho dos animais em pastejo e, ademais, pode ser influenciada pela duração do período de diferimento e pela adubação nitrogenada. O objetivo com este trabalho foi avaliar as características estruturais da Brachiaria brizantha syn. Urochloa brizantha cv. Piatã (capim-piatã) diferida por dois períodos (79 e 127 dias) e com quatro doses de nitrogênio (0, 40, 80 e 120 kg ha-1) na Zona da Mata de Minas Gerais, Brasil. O experimento foi em blocos completos casualizados e em esquema de parcela subdividida, com três repetições. O período de 127 dias de diferimento resultou em maiores massa de forragem, índice de tombamento, porcentagem e densidade volumétrica de colmo, porém em menores número de perfilho, porcentagem e densidade volumétrica de lâmina foliar viva. O incremento da dose de N reduziu a porcentagem de lâmina foliar viva na massa de forragem do capim-piatã diferido por 127 dias. Porém, com o período de 79 dias, a adubação aumentou a densidade volumétrica de lâmina foliar viva e reduziu a porcentagem de colmo na massa de forragem. Como recomendação preliminar, o capim-piatã pode ser diferido por 79 dias e com adubação de até 90 kg ha-1 de N na região da Zona da Mata de Minas Gerais, Brasil.(AU)

Chromium supplementation modulates glucose metabolism in heatstressed Girolando dairy cows / Suplementação com cromo modula o metabolismo de glicose de vacas Girolando sob estresse térmico pelo calor

The objective of this study was to evaluate the effects of dietary chromium supplementation on hepatic gene expression of lactating Girolando (Holstein x Gyr) cows under heat stress conditions in climatic chamber. Thirty-six ¾ Holstein x Gyr lactating cows were used, based on a 2x3 factorial scheme, to evaluate the effects of two diets (0 vs 0.50 mg of organic chromium kg-¹ dry matter) and three environmental conditions (ECs): heat stress conditions in climatic chamber with ad libitum feeding (HS), a thermoneutral environment with ad libitum feeding (TN), and a pair-fed group in a thermoneutral environment (PF). Under HS group, the expression levels of glucose transporter 2(GLUT2), glucose-6-phosphatase (G6Pase), and growth hormone receptor (rGH) were down regulated (P < 0.05) in chromium-supplemented cows compared to those in cows fed the control diet. GLUT2 expression was upregulated (P = 0.02) in the HS group and insulin-like growth factor 1 (IGF1) was downregulated (P < 0.01) in the PF group in cows fed the control diet compared to the expressionin the TN group. No differences were observed between the ECs in terms of relative abundances of GLUT2, phosphoenolpyruvate carboxykinase (PEPCK), G6Pase, rGH, and IGF1 transcripts among the chromium-supplemented cows (P > 0.05). Heat stress caused changes in the expression of genes related to glucose metabolism, and organic chromium could modulate glucose metabolism in animals under heat stress conditions to some extent.(AU)

O objetivo deste estudo foi avaliar o efeito da suplementação com cromo na dieta na expressão gênica hepática de vacas Girolando (Holstein x Gyr) sob estresse térmico pelo calor em câmara climática. Foram utilizadas 36 vacas da raça Holandês x Gir, em um esquema fatorial 2x3, para avaliar duas dietas (0 vs 0,50 mg de cromo orgânico kg-1 de matéria seca) e três condições ambientais: condições de estresse térmico pelo calor em câmara climática com alimentação ad libitum (HS), ambiente termoneutro com alimentação ad libitum (TN) e um grupo com alimentação restrita em um ambiente termoneutro (PF). No grupo HS, as expressões de transportador de glicose 2 (GLUT2), glicose-6-fosfatase (G6Pase) e receptor de hormônio de crescimento (rGH) foram reguladas negativamente (P < 0,05) nas vacas suplementadas com cromo orgânico em comparação com as vacas alimentadas com a dieta controle. A expressão de GLUT2 foi regulada positivamente (P < 0,02) no grupo HS e o fator de crescimento semelhante à insulina 1 (IGF1) foi regulado negativamente (P < 0,01) no grupo PF em comparação como grupo TN para as vacas alimentadas com a dieta controle. Não foram observadas diferenças entre as diferentes condições ambientais na abundância relativa de GLUT2, fosfoenolpiruvato carboxiquinase (PEPCK), G6Pase, rGH e transcritos de IGF1 para as vacas suplementadas com cromo orgânico(P > 0.05). O estresse térmico pelo calor causou alterações na expressão de genes relacionados ao metabolismo da glicose, e o cromo orgânico pode modular o metabolismo da glicose em animais sob essas condições de estresse pelo calor.(AU)

Como a idade do perfilho e a adubação nitrogenada modificam as características estruturais do capim-marandu diferido? / How do age tiller and nitrogen fertilizing modify the structural characteristics of deferred marandu palisade grass?

Este estudo avaliou as características estruturais de perfilhos, com diferentes idades, do capim-marandu (Urochloa brizantha cv. Marandu) diferido e adubado. Foram avaliadas duas doses de nitrogênio (baixa, 50 kg ha-1 e alta, 200 kg ha-1), três categorias de idades de perfilhos (jovem, 4 meses;), além de dois períodos (início, 1 a 45 dias e fim, 46 a 90 dias) durante o diferimento. O delineamento experimental foi inteiramente casualizado, em esquema de parcela sub-subdividida, com quatro repetições. De modo geral, o perfilho jovem apresentou melhor composição morfológica (40,0% de lâmina foliar viva, P=0,0074; 39,2% de colmo vivo, P=0,0208; e 20,8% de lâmina foliar morta, P=0,0155), enquanto que o perfilho velho teve pior morfologia (7,3% de lâmina foliar viva; 54,8% de colmo vivo; e 38,0% de lâmina foliar morta) ao término do período de diferimento. A percentagem de perfilho velho foi maior (P=0,0019) no fim (54,0%) do que no início (43,4%) do período de diferimento. No início do diferimento o dossel adubado com alta dose de N apresentou (P=0,0447) maior percentual de perfilho jovem (36,8%) do que aquele sob baixa dose de N (28,6%). No fim do período de diferimento, tanto a alta como a baixa dose de nitrogênio resultaram (P=0,0276) em valores similares de perfilho jovem no dossel diferido (24,3%, em média). A maior dose de N, parcelada antes do período de diferimento, aumenta o percentual de perfilho jovem durante a fase inicial do diferimento. O perfilho jovem tem melhores características estruturais do que os perfilhos maduros e velhos.

The structural characteristics of tillers with different ages of deferred and fertilized marandu palisade grass (Urochloa brizantha cv. Marandu) were evaluated. Two doses of nitrogen (low, 50 kg ha-1 and high, 200 kg ha-1), three age categories of tillers (young, 4 months), and to two periods (beginning, 1 to 45 days and end, 46 to 90) during deferral were evaluated. The experimental design was completely randomized, in a subsubdivide plot scheme, with four replications. In general, young tiller had a better morphological composition (40.0% live leaf, P=0.0074; 39.2% live stem, P=0.0208; and 20.8% dead leaf blade, P=0.0155), while the old tiller had a worse morphology (7.3% live leaf, 54.8% live stem and 38.0% leaf dead) at the end of the deferral period. The percentage of old tillers was higher (P=0.0019) at the end (54.0%) than at the beginning (43.4%) of the deferment period. At the beginning of the deferment the canopy fertilized with high dose of N presented (P=0.0447) a higher percentage of young tiller (36.8%) than that under low N dose (28.6%). At the end of the deferment period, both the high and the low nitrogen doses resulted (P=0.0276) in similar values of young tiller in the deferred canopy (24.3%, on average). The higher N dose, plotted before the deferral period, increases the percentage of young tiller during the initial phase of deferment. Young tiller has better structural characteristics than mature and old tiller.

Como a idade do perfilho e a adubação nitrogenada modificam as características estruturais do capim-marandu diferido? / How do age tiller and nitrogen fertilizing modify the structural characteristics of deferred marandu palisade grass?

Este estudo avaliou as características estruturais de perfilhos, com diferentes idades, do capim-marandu (Urochloa brizantha cv. Marandu) diferido e adubado. Foram avaliadas duas doses de nitrogênio (baixa, 50 kg ha-1 e alta, 200 kg ha-1), três categorias de idades de perfilhos (jovem, <2 meses; maduro, 2 a 4 meses; e velho, > 4 meses;), além de dois períodos (início, 1 a 45 dias e fim, 46 a 90 dias) durante o diferimento. O delineamento experimental foi inteiramente casualizado, em esquema de parcela sub-subdividida, com quatro repetições. De modo geral, o perfilho jovem apresentou melhor composição morfológica (40,0% de lâmina foliar viva, P=0,0074; 39,2% de colmo vivo, P=0,0208; e 20,8% de lâmina foliar morta, P=0,0155), enquanto que o perfilho velho teve pior morfologia (7,3% de lâmina foliar viva; 54,8% de colmo vivo; e 38,0% de lâmina foliar morta) ao término do período de diferimento. A percentagem de perfilho velho foi maior (P=0,0019) no fim (54,0%) do que no início (43,4%) do período de diferimento. No início do diferimento o dossel adubado com alta dose de N apresentou (P=0,0447) maior percentual de perfilho jovem (36,8%) do que aquele sob baixa dose de N (28,6%). No fim do período de diferimento, tanto a alta como a baixa dose de nitrogênio resultaram (P=0,0276) em valores similares de perfilho jovem no dossel diferido (24,3%, em média). A maior dose de N, parcelada antes do período de diferimento, aumenta o percentual de perfilho jovem durante a fase inicial do diferimento. O perfilho jovem tem melhores características estruturais do que os perfilhos maduros e velhos.(AU)

The structural characteristics of tillers with different ages of deferred and fertilized marandu palisade grass (Urochloa brizantha cv. Marandu) were evaluated. Two doses of nitrogen (low, 50 kg ha-1 and high, 200 kg ha-1), three age categories of tillers (young, <2 months, mature, 2 to 4 months, and old,> 4 months), and to two periods (beginning, 1 to 45 days and end, 46 to 90) during deferral were evaluated. The experimental design was completely randomized, in a subsubdivide plot scheme, with four replications. In general, young tiller had a better morphological composition (40.0% live leaf, P=0.0074; 39.2% live stem, P=0.0208; and 20.8% dead leaf blade, P=0.0155), while the old tiller had a worse morphology (7.3% live leaf, 54.8% live stem and 38.0% leaf dead) at the end of the deferral period. The percentage of old tillers was higher (P=0.0019) at the end (54.0%) than at the beginning (43.4%) of the deferment period. At the beginning of the deferment the canopy fertilized with high dose of N presented (P=0.0447) a higher percentage of young tiller (36.8%) than that under low N dose (28.6%). At the end of the deferment period, both the high and the low nitrogen doses resulted (P=0.0276) in similar values of young tiller in the deferred canopy (24.3%, on average). The higher N dose, plotted before the deferral period, increases the percentage of young tiller during the initial phase of deferment. Young tiller has better structural characteristics than mature and old tiller.(AU)

Morphogenesis of age groups of Marandu palisade grass tillers deferred and fertilised with nitrogen / Morfogênese de faixas etárias de perfilhos do capim-marandu diferido e adubado com nitrogênio

Nitrogen fertilisation allows to modulate the growth of forage canopy during the deferment period. We evaluated the morphogenic characteristics, during the initial and final phases of the deferment period, of young, mature and old tillers of Brachiaria brizantha cv. Marandu. fertilised with low and high doses of nitrogen (N). The design was completely randomised, in a subdivided plot scheme, with four replications. Leaf appearance rate (LAR) and leaf elongation rate (LER) were higher in young tillers than in old ones. At the beginning of the deferment period, the canopy under high N doses had higher LAR and LER values. At the end of the deferment period, we observed no difference between the doses of N for LAR. The stem elongation rate was higher at the beginning than at the end of the deferment period, while the leaf senescence rate (LSR) was higher at higher N doses when compared to lower doses. The highest LSR values and leaf lifespan were observed at the end of the deferment period. The greatest number of young tillers occurred at the beginning of the deferment period and with the highest N dose. In this sense, higher N doses, applied during the active growth period and before the deferment period, favour he maintenance of a larger number of young tillers, which have a greater growth potential.

A adubação nitrogenada permite modular o crescimento do dossel forrageiro durante o período de diferimento. Objetivou-se avaliar, durante as fases inicial e final do período de diferimento, as características morfogênicas de perfilhos jovens, maduros e velhos da Brachiaria brizantha cv. Marandu adubada com doses baixa e alta de nitrogênio (N). O delineamento foi inteiramente casualizado, em esquema de parcela subsubdivida, com quatro repetições. As taxas de aparecimento (TApF) e de alongamento foliar (TAlF) foram maiores em perfilhos jovens do que nos velhos. No início do diferimento o dossel sob alta dose de N teve maiores TApF e TAlF. No fim do diferimento não houve diferença entre as doses de N para TAlF. A taxa de alongamento de colmo foi superior no início do que no fim do diferimento. A taxa de senescência foliar (TSeF) foi maior com a alta dose de N, quando comparado à baixa dose. As maiores TSeF e duração de vida da folha ocorreram no fim, em comparação ao início do diferimento. O maior número de perfilhos jovens ocorreu no início do diferimento e com a maior dose de N. A maior dose N, parcelada durante o período de crescimento ativo da planta, antes do diferimento, favorece a manutenção de maior número de perfilho jovem, o qual tem maior potencial de crescimento.

Morphogenesis of age groups of Marandu palisade grass tillers deferred and fertilised with nitrogen / Morfogênese de faixas etárias de perfilhos do capim-marandu diferido e adubado com nitrogênio

Nitrogen fertilisation allows to modulate the growth of forage canopy during the deferment period. We evaluated the morphogenic characteristics, during the initial and final phases of the deferment period, of young, mature and old tillers of Brachiaria brizantha cv. Marandu. fertilised with low and high doses of nitrogen (N). The design was completely randomised, in a subdivided plot scheme, with four replications. Leaf appearance rate (LAR) and leaf elongation rate (LER) were higher in young tillers than in old ones. At the beginning of the deferment period, the canopy under high N doses had higher LAR and LER values. At the end of the deferment period, we observed no difference between the doses of N for LAR. The stem elongation rate was higher at the beginning than at the end of the deferment period, while the leaf senescence rate (LSR) was higher at higher N doses when compared to lower doses. The highest LSR values and leaf lifespan were observed at the end of the deferment period. The greatest number of young tillers occurred at the beginning of the deferment period and with the highest N dose. In this sense, higher N doses, applied during the active growth period and before the deferment period, favour he maintenance of a larger number of young tillers, which have a greater growth potential.(AU)

A adubação nitrogenada permite modular o crescimento do dossel forrageiro durante o período de diferimento. Objetivou-se avaliar, durante as fases inicial e final do período de diferimento, as características morfogênicas de perfilhos jovens, maduros e velhos da Brachiaria brizantha cv. Marandu adubada com doses baixa e alta de nitrogênio (N). O delineamento foi inteiramente casualizado, em esquema de parcela subsubdivida, com quatro repetições. As taxas de aparecimento (TApF) e de alongamento foliar (TAlF) foram maiores em perfilhos jovens do que nos velhos. No início do diferimento o dossel sob alta dose de N teve maiores TApF e TAlF. No fim do diferimento não houve diferença entre as doses de N para TAlF. A taxa de alongamento de colmo foi superior no início do que no fim do diferimento. A taxa de senescência foliar (TSeF) foi maior com a alta dose de N, quando comparado à baixa dose. As maiores TSeF e duração de vida da folha ocorreram no fim, em comparação ao início do diferimento. O maior número de perfilhos jovens ocorreu no início do diferimento e com a maior dose de N. A maior dose N, parcelada durante o período de crescimento ativo da planta, antes do diferimento, favorece a manutenção de maior número de perfilho jovem, o qual tem maior potencial de crescimento.(AU)

Effects of size and apical or basal portions of the tiller on the morphology of elephant grass

This work was developed to evaluate the effects of the size and the apical and basal portions of the tiller on the morphology of elephant grass (Pennisetum purpureum cv. Napier). Two experiments were performed. In the first one, the characteristics were evaluated of basal tiller with four sizes (80, 120, 160 and 200 cm). A completely randomized design was used, with three replications. In the second experiment, the morphology of the basal and apical portions, from small tillers (100 cm) and large (180 cm) elephant grass were evaluated. The design was completely randomized and the split-plot scheme was used, with three replications. The numbers of living and dead leaves, stem and leaf lengths, leaf blade width, and tiller mass increased linearly with elephant grass size. The basal portion of the elephant grass tiller had a higher mass (56.2 g), higher percentages of leaf dead (2.1%) and live stem (89.5%), and a lower percentage of live leaf blade (23.4%) than the apical portion. With the increase in size, the elephant grass tiller presents a higher percentage of structural tissues and with a higher stage of development, as well as a lower percentage of photosynthetic tissues. The apical portion of the elephant grass tiller has a higher percentage of photosynthetic tissues and a lower percentage of structural tissues and a higher stage of development compared to the basal portion of the tiller.

Este trabalho foi desenvolvido para avaliar os efeitos do tamanho e das porções apical e basal do perfilho sobre a morfologia do capim-elefante (Pennisetum purpureum cv. Napier). Dois experimentos foram realizados. No primeiro, foram avaliadas as características dos perfilhos basilares com quatro tamanhos (80, 120, 160 e 200 cm). Adotou-se o delineamento inteiramente casualizado, com três repetições. No segundo experimento, foi avaliada a morfologia das porções basal e apical, oriundas de perfilhos pequeno (100 cm) e grande (180 cm) do capim-elefante. Adotou-se o delineamento inteiramente casualizado e o esquema de parcela subdividida, com três repetições. Os números de folhas vivas e mortas, os comprimentos do colmo e da lâmina foliar, a largura da lâmina foliar, e a massa do perfilho aumentaram linearmente com o tamanho do perfilho de capim-elefante. A porção basal do perfilho de capim-elefante possuiu maior massa (56,2 g), superiores percentagens de lâmina foliar morta (2,1%) e de colmo vivo (89,5%), e menor percentagem de lâmina foliar viva (23,4%) do que a porção apical. Com o incremento do tamanho, o perfilho de capim-elefante apresenta maior percentagem de tecidos estruturais e com maior estádio de desenvolvimento, bem como inferior percentagem de tecidos fotossintéticos. A porção apical do perfilho de capim-elefante possui superior percentagem de tecidos fotossintéticos e menor percentagem de tecidos estruturais e com maior estádio de desenvolvimento, em comparação à porção basal do perfilho.