Resumo
This study evaluated different crosses for sustainable beef production in the Meio-Norte, Brazil. Thirty-four cattle [seven Curraleiro Pé-duro (CPD), six Nellore (NEL), seven ½ ½ , seven » » ½ , and seven » » ½ ] were evaluated on natural pastures in the states of Piauí and Maranhão. The animals were weighed at birth (BW); weaning (WW); 12 (W12), 18 (W18), and 24 months (W24); and slaughter (SW). The morphometric measurements of rump height (RH), withers height (WH), body length (BW), and heart girth (HG) were assessed. Hot carcass weight (HCW), cold carcass weight (CCW), loin-eye area (LEA), backfat thickness (BFT), carcass dressing percentage (DP), water-holding capacity (WHC), cooking loss (CL), shear force (SF), pH, meat color (L*M, a*M, and b*M), and fat color (L*F, a*F, and b*F) were also analyzed. The three-cross animals (F2A and F2S) showed heavier weights from weaning to slaughter as well as higher HCW and CCW. The three-cross cattle produced less methane per kg of meat. The lack of differences between the NEL, F1, F2A, and F2S animals indicates that crossbreeding did not increase their size, which could be detrimental to reproductive efficiency. Loin-eye area, BFT, and DP differed between the genetic groups, with the highest LEA obtained by F2A. Backfat thickness and DP were low in all animals, suggesting a need for increased carcass fatness. Water-holding capacity, CL, SF, pH, a*F, b*F, L*M, and a*M did not differ; therefore, crossbreeding did not affect qualitative or visual aspects of meat and fat. The use of crosses in meat production systems in the Meio-Norte region of Brazil is a viable option to improve sustainability. In this respect, three-cross animals have the best performance.(AU)
Assuntos
Bovinos/fisiologia , Carne/análise , Constituição Corporal , BrasilResumo
The aim of this research was to evaluate the effects of supplementation of maturation medium withcaptopril in oocyte in vitro maturation (IVM). 470 CCOs were recovered and classified into grades I and II,being divided into four groups: G1 (n = 56), that was control group; G2 (n = 152) 20 mM of captopril; G3 (n =126) 40 uM of captopril; and G4 (n = 136) 80 uM of captopril, then these were subsequently submitted to IVMprocess for 24 hours at 38.5°C in 5% CO2. After 24 hours of maturation, oocytes were denuded and evaluatedfor the first polar body extrusion and were, therefore, considered matured. The addition of the Captopril inmedium of oocytes maturation has not improved the IVM rate.(AU)
Assuntos
Animais , Feminino , Bovinos , Captopril/química , Técnicas de Maturação in Vitro de Oócitos/instrumentação , Técnicas de Maturação in Vitro de Oócitos/veterinária , AngiotensinasResumo
This study was conducted to evaluate the effect of different concentrations of inhibitor antipain serineprotease (10 g, 50 ug and 100 ug) in supplementing the dilutive of bovine semen freezing. Thirty-six ejaculatedfour Curraleiro Pé-Duro cattle were used for cryopreservation. The epifluorescence microscopy was used todetermine the acrosomal integrity and acrosome reaction rate was calculated after sperm were incubated for sixhours heparin. Spermatozoa cryopreserved in the presence of antipain significantly increased acrosomalintegrity, as they were able to complete the acrosome reaction in vitro. In conclusion, the inhibitor is antipainserine protease is able to preserve the integrity of cryopreserved sperm acrosomal cattle.(AU)
Assuntos
Animais , Masculino , Bovinos , Inibidores de Serina Proteinase/análise , Antipaína , Criopreservação , Bovinos , EspermatozoidesResumo
This study was conducted to evaluate the effect of different plasminogen activator inhibitor 1concentrations (70 ƞg, 140 and 210 ƞg ƞg) in the kinetic parameters of sperm cryopreserved of Curraleiro FootHard bulls. Sperm kinetics were analyzed by means of a computer aided system (CASA). The variables evaluatedwere: (MT-%) (MOP-um / s), (VCL - um / s), (VSL - um / s), (VAP-um / s), (LIN -%) ( Str-%) (ALH - uM)Wobble (WOB -%) and (BCF-Hz). The cryopreserved sperm in the presence of the inhibitor of plasminogenactivator 1 in a concentration of 210 ƞg decreased velocity parameters in a straight line (VSL - um / s) andlinearity (LIN -%). In conclusion, supplementation of the Inhibitor of plasminogen activator 1 (PAI-1),cryopreservation of bovine semen does not improve the kinetic parameters as compared to the control.(AU)
Assuntos
Animais , Masculino , Bovinos , Inibidor 1 de Ativador de Plasminogênio/efeitos adversos , Inibidor 1 de Ativador de Plasminogênio/análise , Imobilizantes dos Espermatozoides/análise , CriopreservaçãoResumo
The aim of this study was to evaluate the effects of supplementation of maturation medium withcaptopril, and its consequences in the bovine maturation, fertilization and embryo development in vitro. 326bovine ovaries from a local slaughterhouse were used. 1101 CCOs were recovered and distributed in fourgroups: G1 (n = 112), that was control group; G2 (n = 322) 20μM of captopril; G3 (n = 367) 40μM ofcaptopril; and G4 (n = 300) captopril 80μM and later submitted to IVM. The matured CCOs were fertilized, andco-incubated. After fertilization, 676 presumptive zygotes were cultured and maintained in the greenhouse for 7days. The total number of viable embryos was 12; 39; 32 and 31, respectively in the experimental groups.Considering the experimental conditions adopted, it was concluded that the addition of the Captopril in mediumof oocytes IVM positively doesnt influence embryonic development, as evidenced by the similar percentages ofembryo production.(AU)
Assuntos
Animais , Bovinos , Desenvolvimento Embrionário , Bovinos/crescimento & desenvolvimento , Captopril/administração & dosagem , Captopril/químicaResumo
This study was conducted to evaluate the effect of different plasminogen activator inhibitor 1concentrations (70 ƞg, 140 and 210 ƞg ƞg) in the kinetic parameters of sperm cryopreserved of Curraleiro FootHard bulls. Sperm kinetics were analyzed by means of a computer aided system (CASA). The variables evaluatedwere: (MT-%) (MOP-um / s), (VCL - um / s), (VSL - um / s), (VAP-um / s), (LIN -%) ( Str-%) (ALH - uM)Wobble (WOB -%) and (BCF-Hz). The cryopreserved sperm in the presence of the inhibitor of plasminogenactivator 1 in a concentration of 210 ƞg decreased velocity parameters in a straight line (VSL - um / s) andlinearity (LIN -%). In conclusion, supplementation of the Inhibitor of plasminogen activator 1 (PAI-1),cryopreservation of bovine semen does not improve the kinetic parameters as compared to the control.
Assuntos
Masculino , Animais , Bovinos , Criopreservação , Imobilizantes dos Espermatozoides/análise , Inibidor 1 de Ativador de Plasminogênio/análise , Inibidor 1 de Ativador de Plasminogênio/efeitos adversosResumo
The aim of this research was to evaluate the effects of supplementation of maturation medium withcaptopril in oocyte in vitro maturation (IVM). 470 CCOs were recovered and classified into grades I and II,being divided into four groups: G1 (n = 56), that was control group; G2 (n = 152) 20 mM of captopril; G3 (n =126) 40 uM of captopril; and G4 (n = 136) 80 uM of captopril, then these were subsequently submitted to IVMprocess for 24 hours at 38.5°C in 5% CO2. After 24 hours of maturation, oocytes were denuded and evaluatedfor the first polar body extrusion and were, therefore, considered matured. The addition of the Captopril inmedium of oocytes maturation has not improved the IVM rate.
Assuntos
Feminino , Animais , Bovinos , Angiotensinas , Captopril/química , Técnicas de Maturação in Vitro de Oócitos/instrumentação , Técnicas de Maturação in Vitro de Oócitos/veterináriaResumo
This study was conducted to evaluate the effect of different concentrations of inhibitor antipain serineprotease (10 g, 50 ug and 100 ug) in supplementing the dilutive of bovine semen freezing. Thirty-six ejaculatedfour Curraleiro Pé-Duro cattle were used for cryopreservation. The epifluorescence microscopy was used todetermine the acrosomal integrity and acrosome reaction rate was calculated after sperm were incubated for sixhours heparin. Spermatozoa cryopreserved in the presence of antipain significantly increased acrosomalintegrity, as they were able to complete the acrosome reaction in vitro. In conclusion, the inhibitor is antipainserine protease is able to preserve the integrity of cryopreserved sperm acrosomal cattle.
Assuntos
Masculino , Animais , Bovinos , Antipaína , Bovinos , Criopreservação , Inibidores de Serina Proteinase/análise , EspermatozoidesResumo
The aim of this study was to evaluate the effects of supplementation of maturation medium withcaptopril, and its consequences in the bovine maturation, fertilization and embryo development in vitro. 326bovine ovaries from a local slaughterhouse were used. 1101 CCOs were recovered and distributed in fourgroups: G1 (n = 112), that was control group; G2 (n = 322) 20μM of captopril; G3 (n = 367) 40μM ofcaptopril; and G4 (n = 300) captopril 80μM and later submitted to IVM. The matured CCOs were fertilized, andco-incubated. After fertilization, 676 presumptive zygotes were cultured and maintained in the greenhouse for 7days. The total number of viable embryos was 12; 39; 32 and 31, respectively in the experimental groups.Considering the experimental conditions adopted, it was concluded that the addition of the Captopril in mediumof oocytes IVM positively doesnt influence embryonic development, as evidenced by the similar percentages ofembryo production.