Avaliação da atividade androgênica e antiandrogênica promovida pelo extrato etanólico da richeria grandis vahl. (phyllanthaceae) em ratos / Richeria grandis Vahl. (Phyllanthaceae) ethanolic extract: androgenic and anti-androgenic activity evaluation in rats

The Richeria grandis known is for its aphrodisiac properties and due to its wide empiric use well as thelack of gender Richeria studies the objective of this study was to evaluate the androgenic and anti-androgenicactivity of this plant. They were used in this study 64 male Wistar rats, castrated, divided into eight groups. Thegroups for the study of androgen activity were treated with boiling water over corn R. grandis ethanolic extractoil at doses of 75, 150 and 300 mg / kg and groups of antiandrogenic activity received corn oil, distilled waterand ethanol extract at doses of 75, 150 and 300 mg / kg associated with testosterone. It was found that theethanol extract of R. grandis in the tested doses did not differ statistically from the negative and positive controlsdid not thus demonstrating androgenic activity or antiandrogenic.(AU)

Avaliação da atividade androgênica e antiandrogênica promovida pelo extrato etanólico da richeria grandis vahl. (phyllanthaceae) em ratos / Richeria grandis Vahl. (Phyllanthaceae) ethanolic extract: androgenic and anti-androgenic activity evaluation in rats

The Richeria grandis known is for its aphrodisiac properties and due to its wide empiric use well as thelack of gender Richeria studies the objective of this study was to evaluate the androgenic and anti-androgenicactivity of this plant. They were used in this study 64 male Wistar rats, castrated, divided into eight groups. Thegroups for the study of androgen activity were treated with boiling water over corn R. grandis ethanolic extractoil at doses of 75, 150 and 300 mg / kg and groups of antiandrogenic activity received corn oil, distilled waterand ethanol extract at doses of 75, 150 and 300 mg / kg associated with testosterone. It was found that theethanol extract of R. grandis in the tested doses did not differ statistically from the negative and positive controlsdid not thus demonstrating androgenic activity or antiandrogenic.

Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos / Angiotensin- (1-7): Effect on in vitro maturation of sheep oocytes

Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro: I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.(AU)

Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos / Angiotensin- (1-7): Effect on in vitro maturation of sheep oocytes

Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro: I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.