Presumed normal hemodynamic values of the arteries in the final third period of gestation in bitches

Background: Doppler ultrasonography enables the investigation of vascular blood flow indexes in gestational assessment,being able to detect vascular resistances that can affect fetal and maternal circulation, such as cases of placental insufficiency, associated with fetal cerebral oxygenation deficit and fetal distress. The study aims to assume hemodynamicallynormal values in the final third of gestation in bitches, of the umbilical, uteroplacental, middle cerebral and internal carotidarteries, correlating the obtained Doppler velocimetric indexes, for the assessment of the feto-placental circulation, andprediction of fetal viability indexes, fetal centralization and probable date of delivery.Materials, Methods & Results: Thirty healthy bitches were examined in the final third of gestation (40-60 days). Thesewere evaluated by Doppler ultrasonography at 2 times (T): T1: between 40-50 days; T2: between 51-60 of gestation.At each time point, the peak systolic velocities (PSV) and end-diastolic velocities (EDV) used to obtain the pulsatility(IP) and resistivity (IR) indexes of the umbilical, uteroplacental, middle cerebral and internal carotid arteries of fetuseswere evaluated. Also, the systole/diastole (S/D) ratio of these vessels was evaluated. The data obtained were subjected toanalysis of variance and Tukey test, using a P value equal to 5%. A significant difference was observed between velocitiesand Doppler velocimetric indexes between the 2 phases of the gestational final third in all studied vessels. There was anincrease in the values of PSV and EDV and a decrease in the indexes, as the probable date of delivery approached. Theanalysis of the umbilical cord IR showed an increase from P1 to P2 (P < 0.05), while the IP decreased at the same time.For all studied variables there was a statistically significant difference (P < 0.05). In the uteroplacental...(AU)

Presumed normal hemodynamic values of the arteries in the final third period of gestation in bitches

Background: Doppler ultrasonography enables the investigation of vascular blood flow indexes in gestational assessment,being able to detect vascular resistances that can affect fetal and maternal circulation, such as cases of placental insufficiency, associated with fetal cerebral oxygenation deficit and fetal distress. The study aims to assume hemodynamicallynormal values in the final third of gestation in bitches, of the umbilical, uteroplacental, middle cerebral and internal carotidarteries, correlating the obtained Doppler velocimetric indexes, for the assessment of the feto-placental circulation, andprediction of fetal viability indexes, fetal centralization and probable date of delivery.Materials, Methods & Results: Thirty healthy bitches were examined in the final third of gestation (40-60 days). Thesewere evaluated by Doppler ultrasonography at 2 times (T): T1: between 40-50 days; T2: between 51-60 of gestation.At each time point, the peak systolic velocities (PSV) and end-diastolic velocities (EDV) used to obtain the pulsatility(IP) and resistivity (IR) indexes of the umbilical, uteroplacental, middle cerebral and internal carotid arteries of fetuseswere evaluated. Also, the systole/diastole (S/D) ratio of these vessels was evaluated. The data obtained were subjected toanalysis of variance and Tukey test, using a P value equal to 5%. A significant difference was observed between velocitiesand Doppler velocimetric indexes between the 2 phases of the gestational final third in all studied vessels. There was anincrease in the values of PSV and EDV and a decrease in the indexes, as the probable date of delivery approached. Theanalysis of the umbilical cord IR showed an increase from P1 to P2 (P < 0.05), while the IP decreased at the same time.For all studied variables there was a statistically significant difference (P < 0.05). In the uteroplacental...

Immunolocalization of angiotensin-(1-7), angiotensin II and angiotensin- converting enzyme 2 in goat ovary / Imunolocalização de angiotensina-(1-7), angiotensina II e enzima-conversora de angiotensina 2 em ovário de cabras

There is increasing evidence as to the participation of the ovarian renin-angiotensin system in important reproductive processes. The inhibition of the angiotensin-converting enzyme (ACE) caused an increase in the rate of ovulation and pregnancy in the artificial insemination protocol has fixed time (TFIA). This study aimed to investigate the presence and location of Ang II, Ang- (1-7) and ACE2 in goat ovaries and the possibility of the involvement of these peptides in previous results. Ten ovaries from goats were collected in a slaughterhouse, washed in buffered PBS, perfused with protease inhibitor solution and processed for immunohistochemistry protocol. The search for peptides was performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cells of antral follicles and corpus luteum was observed. Antral follicles (theca cells), corpus luteum and oocyte cytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strong immunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. In this study, for the first time, the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, suggesting that there is participation in follicular development, oocyte maturation and corpus luteum development.

Há evidências crescentes quanto à participação do sistema renina-angiotensina ovariano em processos reprodutivos importantes. A inibição da enzima conversora de angiotensina (ECA) ocasionou aumento na taxa de ovulação e gravidez no protocolo de inseminação artificial por tempo fixo (TFIA). Este estudo teve como objetivo investigar a presença e localização de Ang II, Ang-(1-7) e ECA2 em ovários de cabras e a possibilidade do envolvimento desses peptídeos em resultados anterio-res. Dez ovários de cabras foram coletados em abatedouro, lavados em PBS tamponado, perfundidos com solução inibidora de protease e processados para protocolo de imunohistoquímica. A busca por peptídeos foi realizada usando o método avidina-bio-tina-peroxidase. Foi observada uma forte imunorreatividade para Ang II em células da teca de folículos antrais e corpo lúteo. Os folículos antrais (células da teca), corpo lúteo e citoplasma do oócito nos folículos antrais iniciais exibiram forte imunor-reatividade para Ang-(1-7). Houve forte imunorreatividade para ECA2 no citoplasma das células luteais e células da teca dos folículos antrais. Neste estudo, pela primeira vez, a presença e localização de Ang II, Ang- (1-7) e ECA2 são relatadas em ovário caprino, sugerindo que há participação no desenvolvimento folicular, maturação oocitária e desenvolvimento do corpo lúteo.

Immunolocalization of angiotensin-(1-7), angiotensin II and angiotensin- converting enzyme 2 in goat ovary / Imunolocalização de angiotensina-(1-7), angiotensina II e enzima-conversora de angiotensina 2 em ovário de cabras

There is increasing evidence as to the participation of the ovarian renin-angiotensin system in important reproductive processes. The inhibition of the angiotensin-converting enzyme (ACE) caused an increase in the rate of ovulation and pregnancy in the artificial insemination protocol has fixed time (TFIA). This study aimed to investigate the presence and location of Ang II, Ang- (1-7) and ACE2 in goat ovaries and the possibility of the involvement of these peptides in previous results. Ten ovaries from goats were collected in a slaughterhouse, washed in buffered PBS, perfused with protease inhibitor solution and processed for immunohistochemistry protocol. The search for peptides was performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cells of antral follicles and corpus luteum was observed. Antral follicles (theca cells), corpus luteum and oocyte cytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strong immunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. In this study, for the first time, the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, suggesting that there is participation in follicular development, oocyte maturation and corpus luteum development.(AU)

Há evidências crescentes quanto à participação do sistema renina-angiotensina ovariano em processos reprodutivos importantes. A inibição da enzima conversora de angiotensina (ECA) ocasionou aumento na taxa de ovulação e gravidez no protocolo de inseminação artificial por tempo fixo (TFIA). Este estudo teve como objetivo investigar a presença e localização de Ang II, Ang-(1-7) e ECA2 em ovários de cabras e a possibilidade do envolvimento desses peptídeos em resultados anterio-res. Dez ovários de cabras foram coletados em abatedouro, lavados em PBS tamponado, perfundidos com solução inibidora de protease e processados para protocolo de imunohistoquímica. A busca por peptídeos foi realizada usando o método avidina-bio-tina-peroxidase. Foi observada uma forte imunorreatividade para Ang II em células da teca de folículos antrais e corpo lúteo. Os folículos antrais (células da teca), corpo lúteo e citoplasma do oócito nos folículos antrais iniciais exibiram forte imunor-reatividade para Ang-(1-7). Houve forte imunorreatividade para ECA2 no citoplasma das células luteais e células da teca dos folículos antrais. Neste estudo, pela primeira vez, a presença e localização de Ang II, Ang- (1-7) e ECA2 são relatadas em ovário caprino, sugerindo que há participação no desenvolvimento folicular, maturação oocitária e desenvolvimento do corpo lúteo.(AU)

Efeito da adição de angiotensina-(1-7) na taxa de estradiol de cabras submetidas a protocolo de sincronização do estro e ovulação / Effect of angiotensin (1-7) on estradiol rate of goats undergoing synchronization protocol of estrus and ovulation

This study evaluated the effect of angiotensin- (1-7) on the estradiol levels of goats submitted to aprotocol of synchronization of estrus and ovulation when applied close to the ovulatory period. The animalswere divided into two experimental groups: Control (n = 10) and Angiotensin- (1-7) (n = 10). All animals weresubmitted to estrus synchronization protocol and on days 12 and 13 the animals received the treatmentsaccording to the experimental groups and blood samples from the external jugular vein were collected every 12hours for estradiol dosing (E2) by the Elisa test. The results were submitted to analysis of variance followed bythe Dunnett's test (P <0.05). No increase in serum estradiol concentration was observed in any of thetreatments. It was concluded that the application of angiotensin- (1-7) during the preovulatory period, with onlytwo applications, was not sufficient to significantly increase estradiol levels.(AU)

Efeito do extrato etanólico das folhas de Myracrodruon urundeuva sobre a contratilidade uterina de ratas Wistar / Effect of ethanol extract of Myracrodruon urundeuva leaves on contractility uterine of Wistar rats

Were used 30 virgin female Wistar rats that were mated with adult male rats and vaginal smears were made to detect pregnancy. The pregnant rats were subdivided into pre-treated group with ethanol extract of M. urundeuva (EEMU) at doses of 125, 250 and 500mg/kg PO for 13 days and the untreated group that receiving distilled water being the extract at concentrations of 125, 250 and 500µg/mL added directly to the bath tub for isolated organ. Rats were euthanized by anesthetic overdose and the uterine strips were placed in the bath tub for isolated organ and subjected to contraction with 80mM KCl, oxytocin (16.8IU/mg) and Carbachol (0.3µM). Treatment with EEMU and its direct application did not interfere with uterine contractility of pregnant rats.

Efeito do extrato etanólico das folhas de Myracrodruon urundeuva sobre a contratilidade uterina de ratas Wistar / Effect of ethanol extract of Myracrodruon urundeuva leaves on contractility uterine of Wistar rats

Were used 30 virgin female Wistar rats that were mated with adult male rats and vaginal smears were made to detect pregnancy. The pregnant rats were subdivided into pre-treated group with ethanol extract of M. urundeuva (EEMU) at doses of 125, 250 and 500mg/kg PO for 13 days and the untreated group that receiving distilled water being the extract at concentrations of 125, 250 and 500µg/mL added directly to the bath tub for isolated organ. Rats were euthanized by anesthetic overdose and the uterine strips were placed in the bath tub for isolated organ and subjected to contraction with 80mM KCl, oxytocin (16.8IU/mg) and Carbachol (0.3µM). Treatment with EEMU and its direct application did not interfere with uterine contractility of pregnant rats.(AU)

Imunolocalização de angiotensina-(1-7), angiotensina II e Enzima Conversora de Angiotensina tipo II em ovário de cabra / Immunolocalization of angiotensin-(1-7), angiotensin II and Angiotensin Converting Enzyme II in goat ovary

There is increasing evidence the participation of ovarian renin-angiotensin system in importantreproductive processes. This study aimed to investigate the presence and location of Ang II, Ang-(1-7) and ACE2in goat ovaries. Ten ovaries from goats killed in slaughterhouse were collected, washed in buffered PBS,perfused with protease inhibitor solution and processed for histology standard protocol. The search of peptideswas performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cellsof antral follicles and corpora lutea was observed. Antral follicles (theca cells), corpora lutea and oocytecytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strongimmunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. For the first time,the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, can regulate folliculardevelopment, oocyte maturation and corpus luteum development.(AU)

Efeito da adição de angiotensina-(1-7) na taxa de estradiol de cabras submetidas a protocolo de sincronização do estro e ovulação / Effect of angiotensin (1-7) on estradiol rate of goats undergoing synchronization protocol of estrus and ovulation

This study evaluated the effect of angiotensin- (1-7) on the estradiol levels of goats submitted to aprotocol of synchronization of estrus and ovulation when applied close to the ovulatory period. The animalswere divided into two experimental groups: Control (n = 10) and Angiotensin- (1-7) (n = 10). All animals weresubmitted to estrus synchronization protocol and on days 12 and 13 the animals received the treatmentsaccording to the experimental groups and blood samples from the external jugular vein were collected every 12hours for estradiol dosing (E2) by the Elisa test. The results were submitted to analysis of variance followed bythe Dunnett's test (P <0.05). No increase in serum estradiol concentration was observed in any of thetreatments. It was concluded that the application of angiotensin- (1-7) during the preovulatory period, with onlytwo applications, was not sufficient to significantly increase estradiol levels.

Imunolocalização de angiotensina-(1-7), angiotensina II e Enzima Conversora de Angiotensina tipo II em ovário de cabra / Immunolocalization of angiotensin-(1-7), angiotensin II and Angiotensin Converting Enzyme II in goat ovary

There is increasing evidence the participation of ovarian renin-angiotensin system in importantreproductive processes. This study aimed to investigate the presence and location of Ang II, Ang-(1-7) and ACE2in goat ovaries. Ten ovaries from goats killed in slaughterhouse were collected, washed in buffered PBS,perfused with protease inhibitor solution and processed for histology standard protocol. The search of peptideswas performed using the avidin–biotin–peroxidase method. A strong immunoreactivity for Ang II in theca cellsof antral follicles and corpora lutea was observed. Antral follicles (theca cells), corpora lutea and oocytecytoplasm in early antral follicles exhibited strong immunoreactivity for Ang-(1-7). There was strongimmunoreactivity for ACE2 in the cytoplasm of luteal cells and theca cells of antral follicles. For the first time,the presence and location of Ang II, Ang-(1-7) and ACE2 are reported in goat ovary, can regulate folliculardevelopment, oocyte maturation and corpus luteum development.

Efeito de um inibidor da Enzima Conversora de Angiotensina sobre a esteroidogênese, e Angiotensina II e Angiotensina-(1-7) em cabras superovuladas / Effect of an angiotensin-converting enzyme inhibitor on steroidogenesis, angiotensin II and angiotensin-(1-7) in superovulated goats

Local renin angiotensin (RAS) system has been described in the ovary, which has been implicated invarious reproductive functions. To evaluate the ovarian RAS in, 13 goats were randomly divided into two groups:Enalapril (n = 7) and control (n = 6). Then, they received superovulation protocol. Enalapril further groupreceived subcutaneously (2mg/kg) enalapril maleate (0.4 mg/kg/day). Blood samples were collected on days 3, 6, 9and 11, and follicular fluid samples from pre-ovulatory ovarian follicles after exposure by celiotomy on D11. AngII in serum and follicular fluid were evaluated on days 9 and 12, Ang-(1-7) on day 12 by HPLC and RIA. E2 andP4 concentrations in serum were determined by ELISA. Ang-(1-7) concentrations in plasma was greater on day9 (P < 0.05), important period for the recruitment and follicular selection. This may indicate that these peptidescan an important function in follicular development and oocyte maturation in superovulated goats.(AU)

Avaliação do efeito do extrato etanólico das vagens de Senna spectabilis no consumo de água e ração de ratos wistar / Evaluation of the effect of ethanol extract of pods of Senna spectabilis on consumption of water and food by Wistar rats

This study aims to elucidate the possible effects of Senna spectabilis on the center of hunger and satiety, analyzing their influence on the inhibitory or stimulatory effect of hunger and thirst in rats. The ethanol extract of Senna spectabilis was administered to Wistar rats at three different doses (250, 500 e 1000 mg/kg) for 32 consecutive days and, at the end of each day, the consumption of food and water was measured. There was no observed any changes of consumption pattern of observed animals.

Avaliação do efeito do extrato etanólico das vagens de Senna spectabilis no consumo de água e ração de ratos wistar / Evaluation of the effect of ethanol extract of pods of Senna spectabilis on consumption of water and food by Wistar rats

This study aims to elucidate the possible effects of Senna spectabilis on the center of hunger and satiety, analyzing their influence on the inhibitory or stimulatory effect of hunger and thirst in rats. The ethanol extract of Senna spectabilis was administered to Wistar rats at three different doses (250, 500 e 1000 mg/kg) for 32 consecutive days and, at the end of each day, the consumption of food and water was measured. There was no observed any changes of consumption pattern of observed animals.(AU)

Efeito de um inibidor da Enzima Conversora de Angiotensina sobre a esteroidogênese, e Angiotensina II e Angiotensina-(1-7) em cabras superovuladas / Effect of an angiotensin-converting enzyme inhibitor on steroidogenesis, angiotensin II and angiotensin-(1-7) in superovulated goats

Local renin angiotensin (RAS) system has been described in the ovary, which has been implicated invarious reproductive functions. To evaluate the ovarian RAS in, 13 goats were randomly divided into two groups:Enalapril (n = 7) and control (n = 6). Then, they received superovulation protocol. Enalapril further groupreceived subcutaneously (2mg/kg) enalapril maleate (0.4 mg/kg/day). Blood samples were collected on days 3, 6, 9and 11, and follicular fluid samples from pre-ovulatory ovarian follicles after exposure by celiotomy on D11. AngII in serum and follicular fluid were evaluated on days 9 and 12, Ang-(1-7) on day 12 by HPLC and RIA. E2 andP4 concentrations in serum were determined by ELISA. Ang-(1-7) concentrations in plasma was greater on day9 (P < 0.05), important period for the recruitment and follicular selection. This may indicate that these peptidescan an important function in follicular development and oocyte maturation in superovulated goats.

Avaliação do estresse hipertérmico de ovelhas gestantes das raças Dorper, Santa Inês e suas mestiças em clima tropical com base nos níveis sorológicos de Triiodotironina (T3) e Tiroxina (T4 Total) / Evaluation of the stress head stress sheep pregnant of the breeds Dorper, Santa Inês and their mixed in a tropical climate based on the serological levels of Triiodotironina (T3) and Thyroxine (T4 Total)

The study objectify to evaluate the adaptability of pregnant sheep of the Dorper, Santa Ines and mixed(Dorper ½ ½ Santa Ines) to the tropical climate through serologic possible modifications of triiodothyronine(T3) and thyroxine (T4). The experiment was conducted at Malhada Vermelha farm in Lagoa Alegre - PI, 21sheep were used in gestational middle third, 7 of Dorper, 7 Santa Ines and 7 mixed (Dorper x Santa Ines), all insatisfactory clinical conditions and under the same management conditions. These were evaluated respiratoryrate, rectal temperature and collected blood samples for hormone dosage four times in each climatic periodMarch, July and October. Later they were measured Thyroxine (Total T4) and triiodothyronine (T3) by ELISA.T3 dosages remained within the normal range for the species, while T4 was below. It was concluded that themost adapted breed is Santa Ines.(AU)

Angiotensina-(1-7): Efeito na maturação in vitro de oócitos ovinos / Angiotensin- (1-7): Effect on in vitro maturation of sheep oocytes

Aimed to evaluate the effect of addition of angiotensin-(1-7) in the middle of in vitro maturation ofsheep oocytes. Seventy-four oocytes of sheep ovaries slaughtered in fridg were matured in vitro: I - control(n=22), II - Ang-(1-7) at 1 uM (n=24), III - Ang-(1-7) at 1 uM+A-779 at 1uM (n=28) in microdropletscontaining 100 µL of maturation medium plus 10% fetal bovine serum under mineral oil in the incubatoratmosphere 5% CO2 in air at 38.5°C for a period of 18 hours. After this period, the oocytes were denuded andobserved the extrusion of the first polar body. Statistical analysis was performed using the t test at 5%probability. The addition of angiotensin-(1-7) at 1 uM, as well as the addition of angiotensin-(1-7) at 1 uMtogether with its specific antagonist A-779 at the same concentration, decrease the in vitro maturation rate ovine oocytes.(AU)

Captopril na produção in vitro de embriões bovinos? / Captopril in production in vitro of embryos bovine?

Objective to evaluate the effect of captopril in vitro production of bovine embryos. 472 COCs were usedfrom abattoir ovaries, which were screened, sorted and selected in grades I and II, and submitted to the PIVprocess. The IVM treatments were added in the following concentrations: T1 - control; T2 - 5 mM of captopril;T3 - 10 uM of captopril; and T4 - 15 captopril uM, the COCs were subjected to IVM, IVF CIV. The results showthat there was no statistically significant difference (P > 0.05) between treatments during maturation. Thevariables studied were cleavage and blastocyst rates. The captopril supplementation did not improve (P > 0.05)G1 cleavage rate - 61.84%; G2 - 71.00%; G3 - 68.87%; and G4 - 56.90%. Supplementation of 20μM ofcaptopril influenced the amount of viable embryos.(AU)

Efeito do captopril na maturação in vitro de oócitos bovinos / Effect of captopril in maturation in vitro of oocyte bovine

This study aimed to evaluate the effect of captopril on the in vitro maturation of oocytes. 1627 COCscattle slaughter houses were used, were subsequently screened, classified into grades I and II, according to themorphological quality, and submitted to the IVM process, distributed in four treatments: T1 - control; T2 - 5μMof captopril; T3 - 10μM of captopril; and T4 - 15μM of captopril, incubated in an incubator at 38.5 ° C with anatmosphere of 5% CO2. After 22 hours, the COCs were stripped to evaluate the rate of maturation, which wereas follows: T1 - 56.60% (120/212); T2 - 60.60% (240/396); T3 - 52.88% (174/329) and T4 - 58.30% (207/355).The results show that there was no statistically significant difference ( P> 0.05) between treatments duringmaturation, concluding that captopril supplementation did not affect the maturation of COCs.(AU)

Eficiência da inseminação artificial em tempo fixo (IATF) associada à detecção de estro por marcação da cauda e presença de muco vaginal no dia da inseminação em vacas Nelore / Efficiency of artificial inseminatio n in fixed time (FTAI) associated with estrus detection by marking the tail and the presence ofvaginal mucus on the day of insemination in Nelore cows

The objective of this study was to evaluate the efficiency of artificial insemination in fixed timeassociated with estrus detection in Nellore cows. They used 917 females, all submitted to estrus synchronizationprotocol and formed two groups: G1 (n = 489), marked on the back with fluorescent dye and then checked if thecroup was blurred or not, and had vaginal mucus or not and G2 (n = 428) were formed by females inseminatedonly synchronized. Pregnancy diagnosis was performed by ultrasound 30 days after insemination. In G1 and G2,pregnancy rates were, respectively, 46.01% and 41.12%. In G1, blurred females (52.19%), not blurred(21.78%), presence of mucus (62.57%) and no mucus (35.97%). Pregnancy rates did not differ between thegroups G1 and G2. In G1, blurred females or mucus had higher rates. It is concluded that the detection ofestrus, insemination favored the pregnancy rate.(AU)

Eficiência da inseminação artificial em tempo fixo (IATF) associada à determinação do diâmetro folicular e concetração de progesterona no dia da inseminação em vacas Nelore / Efficiency of artificial insemination in fixed time (FTAI) associated with determination of follicular diameter and progesterone concentration on the day of insemination in Nellore cows

The objective of this study was to evaluate the association of IATF with FD on the reproductiveefficiency of Nellore cows. It was used 917 females submitted to estrus synchronization protocol, with P4 deviceplaced and applied 2mL BE in D0; In D9 removed the device, applied 2.5mL of PG, 0.3mL of CE and 300uI ofeCG. The inseminations made on D11, 48-54 hours after withdrawal from the device. Blood collections weremade on D0 and D11. G1 (n = 489) were formed, with synchronized females and, prior to insemination,measured FD, classified as large, medium or small; The G2 (n = 428), by females only synchronized andinseminated. Gestation rates in G1 and G2 were 46.01% and 41.12%, and did not differ between groups. In theG1, females with large and medium follicles rates did not differ, since females with small follicles had lowerrates. P4 in the D0 indicated 47.19% of females in the follicular phase and 46.67% in the progesterone phase;In D11, the means of P4, considered low, medium or high, had rates of 82.14, 57.14 and 8.57%, respectively. Itis concluded that females with small follicles or with P4 raised to insemination have low pregnancy rates.(AU)