Resumo

Mesotheliomas in cattle are often described as isolated case reports, and investigations of multiple cases within the same bovine herd are lacking. A series of cases of malignant epithelial mesothelioma, tubulopapilary type, is described in five 15 to 21-year-old Red Sindhi cows from the same herd. Clinical signs included three to eight months of progressive emaciation, dehydration, subcutaneous edema of the lower extremities, and abdominal distension. Grossly, severe subcutaneous edema and hydroperitoneum were noted. Multiple organs' parietal and visceral serosal surfaces had multifocal to coalescing yellow, firm, sessile nodules ranging from 0.1 to 29.0cm. Similar free nodules floated in the peritoneal fluid. Histologically, the masses comprised a layer of cubic to columnar neoplastic cells forming papillary or cystic proliferation supported by a dense fibrovascular stroma. Neoplastic cells had strong and diffuse cytoplasmic immunolabeling for pan-cytokeratin but were negative for cytokeratin 7 and vimentin. Ultrastructurally, neoplastic cells had delicate microvilli and tight and anchoring junctions. Within the cytoplasm, a moderate amount of loose aggregate of intermediary filament with small mitochondria was observed. Epidemiological investigation evidenced endogamy in this herd. Asbestos exposure was not detected. The diagnosis was based on clinical, gross, histological, and immunohistochemical findings and confirmed by transmission electron microscopy features. A definitive underlying etiology remains unknown.

Mesotelioma em bovinos são frequentemente relatados como casos isolados, descrições de múltiplos casos no mesmo rebanho bovino não foram encontrados. Descreve-se uma série de casos de mesotelioma epitelial maligno, tipo tubulopapilar, em cinco vacas Red Sindi de 15 a 21 anos de idade do mesmo rebanho. Os sinais clínicos incluíram emagrecimento progressivo, desidratação, edema subcutâneo das extremidades dos membros e distensão abdominal em um curso clínico que variou de três a oito meses. Macroscopicamente, observou-se edema subcutâneo acentuado e hidroperitônio. Nas serosas parietais e viscerais de múltiplos órgãos haviam nódulos multifocais a coalescentes amarelo-claros, firmes e sésseis que variavam de 0,1 a 29,0 centímetros de diâmetro. Nódulos livres semelhantes também flutuavam no líquido peritoneal. Histologicamente, as massas eram compostas por uma camada de células cúbicas a colunares formando proliferação papilar ou cística sustentada por estroma fibrovascular denso. As células neoplásicas apresentavam imunomarcação citoplasmática forte e difusa para pan-citoqueratina, mas eram negativas para citoqueratina 7 e vimentina. Ultraestruturalmente, as células neoplásicas apresentavam delicadas microvilosidades e junções comunicantes e de ancoragem. No citoplasma observou-se moderada quantidade de agregados de filamentos intermediários soltos e pequenas mitocôndrias. A investigação epidemiológica revelou que não houve inserção de bovinos de outros rebanhos por mais de 30 anos e evidenciou endogamia. Não foram encontradas possíveis fontes de amianto para os bovinos deste rebanho. O diagnóstico de mesotelioma foi baseado em características clínicas, macroscópicas, histológicas, imuno-histoquímicas e confirmado pelos achados de microscopia eletrônica. A etiologia permanece desconhecida.

Resumo

A 12-year-old female mixed-breed dog presented with lameness, pain, and an enlarged, non-ulcerated, nodular mass in the region proximal to the tarsal joint of the right pelvic limb. Surgical excision was performed, revealing a 6.5 cm mass adherent to the deep flexor tendon and adjacent tissues. The cut section had cysts filled with blackened clotted material, which exuded reddish serous fluid. Microscopically, the cysts were filled with red blood cells and were either denuded or covered by synoviocytes. In addition, the mass was characterized by marked fibrovascular connective tissue associated with siderophages and multinucleated giant cells. These findings were consistent with those of pigmented villonodular tenosynovitis, a rare condition affecting several animal species and humans.

Uma cadela de 12 anos, sem raça definida, apresentou claudicação, algia e aumento de volume não ulcerado, de aspecto nodular, na região proximal à articulação do tarso do membro pélvico direito. A excisão cirúrgica foi optada e revelou uma massa de 6,5 cm de diâmetro, aderida ao tendão flexor profundo e aos tecidos adjacentes. Ao corte, exsudava líquido seroso avermelhado e cistos preenchidos por material coagulado enegrecido foram observados. Microscopicamente, a massa apresentava formações císticas frequentemente preenchidas por hemácias, que encontravam-se ora revestidas por sinoviócitos, ora desnudas. Havia ainda acentuada quantidade de tecido fibrovascular associado a siderófagos e células gigantes multinucleadas. Esses achados foram consistentes com tenossinovite vilonodular pigmentada, uma rara condição que afeta diversas espécies de animais e humanos.

Resumo

ABSTRACT: A 12-year-old female mixed-breed dog presented with lameness, pain, and an enlarged, non-ulcerated, nodular mass in the region proximal to the tarsal joint of the right pelvic limb. Surgical excision was performed, revealing a 6.5 cm mass adherent to the deep flexor tendon and adjacent tissues. The cut section had cysts filled with blackened clotted material, which exuded reddish serous fluid. Microscopically, the cysts were filled with red blood cells and were either denuded or covered by synoviocytes. In addition, the mass was characterized by marked fibrovascular connective tissue associated with siderophages and multinucleated giant cells. These findings were consistent with those of pigmented villonodular tenosynovitis, a rare condition affecting several animal species and humans.

RESUMO: Uma cadela de 12 anos, sem raça definida, apresentou claudicação, algia e aumento de volume não ulcerado, de aspecto nodular, na região proximal à articulação do tarso do membro pélvico direito. A excisão cirúrgica foi optada e revelou uma massa de 6,5 cm de diâmetro, aderida ao tendão flexor profundo e aos tecidos adjacentes. Ao corte, exsudava líquido seroso avermelhado e cistos preenchidos por material coagulado enegrecido foram observados. Microscopicamente, a massa apresentava formações císticas frequentemente preenchidas por hemácias, que encontravam-se ora revestidas por sinoviócitos, ora desnudas. Havia ainda acentuada quantidade de tecido fibrovascular associado a siderófagos e células gigantes multinucleadas. Esses achados foram consistentes com tenossinovite vilonodular pigmentada, uma rara condição que afeta diversas espécies de animais e humanos.

Resumo

Background: Pituitary pars intermedia (PI) adenoma is a benign adenohypophysis neoplasm, rare in Brazil, which maycompress adjacent structures and lead to dysfunctions of the endocrine organs. The most affected species are equines,particularly aged animals. This neoplastic disease is often associated with Cushing’s syndrome, when the pituitaryadrenocortical axis is affected. However, this neoplasm is seldom associated with clinical blindness. This paper describesa case of pituitary pars intermedia (PI) adenoma which caused blindness in a mare.Case: An emaciated, blind mare that had difficulty finding water and food was referred to “Hospital Veterinário de GrandesAnimais” of “Universidade Federal Rural do Rio de Janeiro”, in Seropédica, state of Rio de Janeiro, Brazil, and later tothe “Setor de Anatomia Patológica” (SAP/UFRRJ) for necropsy. During necropsy it was found that the animal’s pituitarygland consisted of a circumscribed globous mass measuring 3.5x2.5x2.5 cm, symmetrical and well delimited at the sellaturcica. Several organs were collected, fixed in 10% formalin and then processed for histological examination. The brainwas subjected to serial cleavage along the path of the vision organs. All collected tissues were stained with hematoxylinand eosin; the pituitary was stained with Schiff Periodic Acid (PAS) to differentiate neoplastic cells and with Luxol FastBlue to evidence demyelination. Histopathology found that the pars intermedia (PI) was thickened and compressed theneurohypophysis and adenohypophysis. Cells were arranged in a disorganized manner or formed follicles or cysts containing eosinophilic material (colloid), with granules strongly positive after staining with PAS. Proliferated cells werelarge, polyhedral to ovoid, and contained clear basophilic granular material. The nuclei were spherical to ovoid and therewere mild anisocytosis and anisocariasis. Cysts were frequently found in the pars intermedia (PI)...

Resumo

Malignant catarrhal fever (MCF) is an infectious, pansystemic and highly fatal disease with wide geographic distribution. The species that are clinically prone to it include cattle, deer and bison. In Brazil, the disease in ruminants and deer is associated with the contact with sheep, especially during labor, when the fetal remains that are eliminated contain the ovine herpesvirus 2 (OvHV-2). The outbreak took place in a conservationist property in the city of Casimiro de Abreu/RJ, which hosted 23 Sambar deer, and, of these, 19 died, showing neurological signs. The deer lived in a location together with 15 male and female meat sheep. A female specimen of the Sambar deer (Rusa unicolor), aged approximately three years, which had presented with neurological clinical signs was referred to necropsy in the Setor de Anatomia Patológica at Universidade Federal Rural do Rio de Janeiro (SAP/UFRRJ). During necropsy, cerebrospinal fluid was sampled for analysis; fragments of several organs were fixated in 10% buffered formalin and processed for histopathological analysis. Fragments of occipital lobe, cerebellum and bulb were collected to perform the polymerase chain reaction (PCR). The diagnosis of this outbreak was based on epidemiological, clinical and pathological findings, and on the amplification of the OvHV-2 DNA through PCR. The histological changes were the base to confirm the MCF case and were characterized by degeneration of vascular endothelial cells, fibrinoid vasculitis, hyperplasia and necrosis of lymphoid organs. However, PCR was an important tool to confirm the diagnosis. MCF as an important disease with nervous symptomatology in deer.(AU)

A febre catarral maligna (FCM) é uma doença infecciosa, com distribuição geográfica ampla, pansistêmica e altamente fatal. As espécies clinicamente suscetíveis incluem bovino, cervo e bisão. No Brasil, a doença em ruminantes e cervídeos está associada ao contato com ovinos, principalmente durante o parto, no qual os envoltórios fetais eliminados contém, em suas secreções, o Herpesvírus ovino-2 (OvHV-2). O surto ocorreu em uma propriedade conservacionista no município de Casimiro de Abreu/RJ, que abrigava 23 cervos exóticos, onde foram registradas a morte de 19 destes, com sinais neurológicos. Os cervos habitavam em um piquete com 15 ovinos de corte, machos e fêmeas. Um exemplar de cervo sambar (Rusa unicolor), fêmea, com aproximadamente três anos de idade, que havia apresentado sinais clínicos neurológicos foi encaminhado para necropsia no Setor de Anatomia Patológica da Universidade Federal Rural do Rio de Janeiro (SAP/UFRRJ). Durante a necropsia foi realizada a coleta de líquido cefalorraquidiano e de fragmentos de lobo occipital, cerebelo e bulbo, para a realização de reação em cadeia da polimerase (PCR). Fragmentos de diversos órgãos foram fixados em formalina 10% tamponada e processados para a análise histopatológica. O diagnóstico do presente surto foi estabelecido com base nos achados epidemiológicos, clínicos, patológicos e na amplificação do DNA do OvHV-2 através da PCR. As alterações histológicas foram a base para confirmar o caso de FCM e caracterizaram-se por degeneração de células endoteliais vasculares, vasculite fibrinoide, hiperplasia dos órgãos linfoides. Contudo, a PCR foi uma ferramenta importante para a confirmação do diagnóstico. Ressalta-se a importância da FCM na lista dos diagnósticos diferenciais de doenças que cursam com sintomatologia nervosa em cervídeos.(AU)

Resumo

Background: Emphysematous rumenitis is a condition characterized by gas filled bubbles or cysts in the ruminal mucosa. Although a similar pathology is reported from swine and humans, the incidence of this disease in cattle seems to be scarce. The etiology of emphysematous rumenitis is uncertain once many factors can be involved in its pathogeny, a single cause is difficult to ascertain. This lesion usually fails to display specific clinical signs; however, some degree of malabsorption and weight loss is expected. We described a case of emphysematous granulomatous submucosal rumenitis in a 2.5-yearold feedlot Nellore steer that presented reduction on daily weight gain.Case: Rumen fragments of a 2.5-year-old Nellore steer were submitted for histopathological evaluation at the Sector of Pathological Anatomy (SAP) of the Rural Federal University of Rio de Janeiro, Brazil. The specimens were part of an experimental trial to evaluate the effect of phosphorus (P) supplementation on nutrient intake, performance and P balance in fifty feedlot Nellore steers. Following a short adaptation period, in which behavioral observations were made twice daily to assess possible adverse factors, all steers were fed with balanced diet for one hundred and sixteen days. The steers were then slaughtered in order to evaluate macroscopic changes in the gastrointestinal system. Rumen and abomasum of each steer was emptied, washed with tap water and grossly examined. One steer presented ruminal lesion, while the other forty-nine did not show any ruminal injury. Throughout the experiment the affected steer showed reduction of daily weight gain; but during clinical examination no additional clinical sign was seen. At gross inspection, a reduction in the amount of muscular and adipose tissue was seen. Multiple coalescent bullous elevated structures measuring three to ten mm diameter were covering about 40% of the ruminal surface.[...]