Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros

Base de dados
Ano de publicação
Intervalo de ano de publicação
1.
Anim. Reprod. (Online) ; 15(2): 108-113, Apr.-June.2018. tab
Artigo em Inglês | VETINDEX | ID: biblio-1461346

Resumo

Bull Semen Collection and Processing Centers (SCPC) have satisfactory control of sperm quality, but commonly lack standardized quality control of hygiene procedures. This study assessed the impact of implementing a Hazard Analysis and Critical Control Points (HACCP) system in a bull SCPC, comparing microbial counts on various steps of semen processing, semen quality and costs across two periods (before and after the HACCP implementation). After surveying all routine activities of the SCPC, control points were identified, preventive measures were designed and corrective actions were employed, whenever necessary. Six months after HACCP implementation, the system was audited and production data covering two similar periods of two consecutive years were compared. Counts of colony forming units in samples collected from artificial vaginas, flexible tubes from the straw filling machine and from fresh and frozen semen after HACCP implementation were lower than during the previous period (P < 0.05). Improved post-thawing sperm motility, membrane integrity and acrosome integrity (P < 0.0001) and reduced rejection of semen batches and frozen doses were observed after HACCP implementation (P < 0.01), resulting in reduced opportunity costs. Thus, the implementation of a HACCP system in a bull SCPC allowed low-cost production of high-quality semen doses with reduced microbial contamination.


Assuntos
Masculino , Animais , Bovinos , Análise do Sêmen/efeitos adversos , Análise do Sêmen/veterinária , Bovinos/genética , Controle de Qualidade
2.
Anim. Reprod. ; 15(2): 108-113, Apr.-June.2018. tab
Artigo em Inglês | VETINDEX | ID: vti-16525

Resumo

Bull Semen Collection and Processing Centers (SCPC) have satisfactory control of sperm quality, but commonly lack standardized quality control of hygiene procedures. This study assessed the impact of implementing a Hazard Analysis and Critical Control Points (HACCP) system in a bull SCPC, comparing microbial counts on various steps of semen processing, semen quality and costs across two periods (before and after the HACCP implementation). After surveying all routine activities of the SCPC, control points were identified, preventive measures were designed and corrective actions were employed, whenever necessary. Six months after HACCP implementation, the system was audited and production data covering two similar periods of two consecutive years were compared. Counts of colony forming units in samples collected from artificial vaginas, flexible tubes from the straw filling machine and from fresh and frozen semen after HACCP implementation were lower than during the previous period (P < 0.05). Improved post-thawing sperm motility, membrane integrity and acrosome integrity (P < 0.0001) and reduced rejection of semen batches and frozen doses were observed after HACCP implementation (P < 0.01), resulting in reduced opportunity costs. Thus, the implementation of a HACCP system in a bull SCPC allowed low-cost production of high-quality semen doses with reduced microbial contamination.(AU)


Assuntos
Animais , Masculino , Bovinos , Bovinos/genética , Análise do Sêmen/efeitos adversos , Análise do Sêmen/veterinária , Controle de Qualidade
3.
Semina ciênc. agrar ; 37(3): 1345-1354, maio/jun. 2016. tab, ilus
Artigo em Inglês | VETINDEX | ID: vti-28315

Resumo

Microorganisms that cause human diseases can contaminate fishes in aquatic environments as well as during their capture, handling, and transport. The purpose of this study was to isolate Vibrio parahaemolyticus and Salmonella enterica from fishes captured in the Lagoa dos Patos estuary that were eviscerated and cleaned for trade. Thirteen fish landing were analyzed and 65 entire fishes and 65 cleaned fishes were studied to determine the presence of V. parahaemolyticus and S. enterica. Bacterial isolates were compared using rep-PCR. V. parahaemolyticus was isolated from one entire Micropogonias furnieri and two entire Mugil platanus, as well as from three eviscerated M. platanus. S. enterica was isolated from two eviscerated Paralichthys orbignyanus. Identical rep-PCR bands from V. parahaemolyticus were observed in entire and eviscerated fishes from the same discharge, suggesting processing failures that neither eliminated the microorganism from the raw material nor prevented cross-contamination. S. enterica was not isolated from entire fishes, presumably because contamination occurred due to hygiene and sanitary failures. Our results showed that M. furnieri and M. platanus captured in the Lagoa dos Patos estuary may host V. parahaemolyticus and that this microorganism, as with S. enterica, may also persist even after the fish is cleaned. This is the first record of the...(AU)


Micro-organismos que causam doenças humanas podem contaminar os peixes no ambiente aquático, durante a captura, manuseio e transporte. O objetivo foi isolar Vibrio parahaemolyticus e Salmonella enterica de peixes capturados no estuário da Lagoa dos Patos e artesanalmente eviscerados e limpos para o comércio. Treze desembarques foram acompanhados e 65 peixes inteiros e 65 limpos foram analisados quanto à presença de V. parahaemolyticus e S. enterica. Os isolados foram comparados entre si através de rep-PCR. V. parahaemolyticusfoi isolado em uma corvina (Micropogonias furnieri)e duas tainhas(Mugil platanus) inteiras e em três M. platanus eviscerados. S. enterica foi isolada apenas de dois linguados (Paralichthys orbignyanus) eviscerados. O mesmo perfil de bandas do V. parahaemolyticus pela rep-PCR foram observados nos peixes inteiros e eviscerados do mesmo desembarque, sugerindo falhas de processamento, o qual não eliminou o micro-organismo da matéria-prima ou não preveniu a contaminação cruzada. Como S. enterica não foi isolada de peixes inteiros, presume-se que a contaminação aconteceu por causa de falhas higiênicas e sanitárias. Os resultados mostraram que os peixes da espécie M. furnieri e M. platanus capturados no estuário da Lagoa dos Patos podem hospedar V. parahaemolyticus e que este micro-organismo, assim como S. enterica, também pode estar presente em peixes eviscerados...(AU)


Assuntos
Animais , Vibrio parahaemolyticus/patogenicidade , Salmonella enterica/patogenicidade , Peixes/microbiologia , Estuários
4.
Braz. J. Microbiol. ; 47(3): 675-679, Jul-Set. 2016. graf, tab
Artigo em Inglês | VETINDEX | ID: vti-23446

Resumo

The need for cleaner sources of energy has stirred research into utilising alternate fuel sources with favourable emission and sustainability such as biodiesel. However, there are technical constraints that hinder the widespread use of some of the low cost raw materials such as pork fatty wastes. Currently available technology permits the use of lipolytic microorganisms to sustainably produce energy from fat sources; and several microorganisms and their metabolites are being investigated as potential energy sources. Thus, the aim of this study was to characterise the process of Staphylococcus xylosus mediated fermentation of pork fatty waste. We also wanted to explore the possibility of fermentation effecting a modification in the lipid carbon chain to reduce its melting point and thereby act directly on one of the main technical barriers to obtaining biodiesel from this abundant source of lipids. Pork fatty waste was obtained from slaughterhouses in southern Brazil during evisceration of the carcasses and the kidney casing of slaughtered animals was used as feedstock. Fermentation was performed in BHI broth with different concentrations of fatty waste and for different time periods which enabled evaluation of the effect of fermentation time on the melting point of swine fat. The lowest melting point was observed around 46 °C, indicating that these chemical and biological reactions can occur under milder conditions, and that such pre-treatment may further facilitate production of biodiesel from fatty animal waste.(AU)


Assuntos
Staphylococcus/crescimento & desenvolvimento , Fermentação , Biocombustíveis/análise , Suínos
5.
Semina ciênc. agrar ; 37(3): 1345-1354, maio/jun. 2016. tab, ilus
Artigo em Inglês | VETINDEX | ID: biblio-1500366

Resumo

Microorganisms that cause human diseases can contaminate fishes in aquatic environments as well as during their capture, handling, and transport. The purpose of this study was to isolate Vibrio parahaemolyticus and Salmonella enterica from fishes captured in the Lagoa dos Patos estuary that were eviscerated and cleaned for trade. Thirteen fish landing were analyzed and 65 entire fishes and 65 cleaned fishes were studied to determine the presence of V. parahaemolyticus and S. enterica. Bacterial isolates were compared using rep-PCR. V. parahaemolyticus was isolated from one entire Micropogonias furnieri and two entire Mugil platanus, as well as from three eviscerated M. platanus. S. enterica was isolated from two eviscerated Paralichthys orbignyanus. Identical rep-PCR bands from V. parahaemolyticus were observed in entire and eviscerated fishes from the same discharge, suggesting processing failures that neither eliminated the microorganism from the raw material nor prevented cross-contamination. S. enterica was not isolated from entire fishes, presumably because contamination occurred due to hygiene and sanitary failures. Our results showed that M. furnieri and M. platanus captured in the Lagoa dos Patos estuary may host V. parahaemolyticus and that this microorganism, as with S. enterica, may also persist even after the fish is cleaned. This is the first record of the...


Micro-organismos que causam doenças humanas podem contaminar os peixes no ambiente aquático, durante a captura, manuseio e transporte. O objetivo foi isolar Vibrio parahaemolyticus e Salmonella enterica de peixes capturados no estuário da Lagoa dos Patos e artesanalmente eviscerados e limpos para o comércio. Treze desembarques foram acompanhados e 65 peixes inteiros e 65 limpos foram analisados quanto à presença de V. parahaemolyticus e S. enterica. Os isolados foram comparados entre si através de rep-PCR. V. parahaemolyticusfoi isolado em uma corvina (Micropogonias furnieri)e duas tainhas(Mugil platanus) inteiras e em três M. platanus eviscerados. S. enterica foi isolada apenas de dois linguados (Paralichthys orbignyanus) eviscerados. O mesmo perfil de bandas do V. parahaemolyticus pela rep-PCR foram observados nos peixes inteiros e eviscerados do mesmo desembarque, sugerindo falhas de processamento, o qual não eliminou o micro-organismo da matéria-prima ou não preveniu a contaminação cruzada. Como S. enterica não foi isolada de peixes inteiros, presume-se que a contaminação aconteceu por causa de falhas higiênicas e sanitárias. Os resultados mostraram que os peixes da espécie M. furnieri e M. platanus capturados no estuário da Lagoa dos Patos podem hospedar V. parahaemolyticus e que este micro-organismo, assim como S. enterica, também pode estar presente em peixes eviscerados...


Assuntos
Animais , Estuários , Peixes/microbiologia , Salmonella enterica/patogenicidade , Vibrio parahaemolyticus/patogenicidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA