Resumo
The status of Babesia spp. infection in dogs from rural areas of São Paulo State, Brazil was studied. For this, 150 animals were examined by blood smears and by PCR; the presence of tick infestation was also investigated. By the blood smear examination, 3 animals (2%) were detected positive and by PCR for Babesia spp. 12 (8%) were positive, with bands visualized in 450 bp. Rhipicephalus sanguineus or Amblyomma spp. were found on 36 (24%) of the 150 dogs. Amblyomma species found were A. cajennense (9/36-25%) and A. ovale (9/36-25%). It was not possible to correlate the presence of R. sanguineus and the infection with Babesia spp. The sequencing of four positive samples demonstrated close identity with B. canis vogeli already characterized in Brazil.
A presença de infecção por Babesia spp. em cães de áreas rurais do Estado de São Paulo, Brasil foi investigada. Para tanto, 150 cães foram examinados por técnicas parasitológicas de esfregaços sanguíneos e moleculares (PCR), e também, foi verificada a presença de carrapatos nestes animais. Pela análise de esfregaços sanguíneos, 3 (2%) dos cães estavam infectados, enquanto pela PCR, 12 (8%) dos animais foram positivos com bandas aproximadas de 450 pares de base (pb). Foram observados 36 (24%) cães infestados com Rhipicephalus sanguineus ou com Amblyomma spp. As espécies de Amblyomma observadas foram A. cajennense (25%) e A. ovale (25%). Não foi possível correlacionar a presença de R. sanguineus com a infecção por Babesia spp. O seqüenciamento de quatro amostras positivas demonstrou alta identidade com B. canis vogeli, já caracterizada no Brasil.
Resumo
Streptococcus suis represents a potential zoonosis risk in personnel working in closecontact with swines. The purpose of this study was to evidence the humancarrier status of S. suis in farmworkers belonging to three different pig farms located in three differentcities of the state of São Paulo, Brazil. Cotton swab samples were taken fromtonsils of 28 workers. The S. suis-like strains were identified bypolymerase chain reaction, confirmed by sequencing and serotyped bycoagglutination technique. For the first time in Brazil, one farm worker wasidentified as a human carrier of S. suis.
Streptococcus suis represents apotential zoonosis risk in personnel working in close contact with swines. Thepurpose of this study was to evidence the human carrier status of S. suis in farm workers belonging tothree different pig farms located in three different cities of the state of SãoPaulo, Brazil. Cotton swab samples were taken from tonsils of 28 workers. The S. suis-like strains were identified bypolymerase chain reaction, confirmed by sequencing and serotyped bycoagglutination technique. For the first time in Brazil, one farm worker was identified as a human carrier of S. suis.
Resumo
Streptococcus suis represents a potential zoonosis risk in personnel working in closecontact with swines. The purpose of this study was to evidence the humancarrier status of S. suis in farmworkers belonging to three different pig farms located in three differentcities of the state of São Paulo, Brazil. Cotton swab samples were taken fromtonsils of 28 workers. The S. suis-like strains were identified bypolymerase chain reaction, confirmed by sequencing and serotyped bycoagglutination technique. For the first time in Brazil, one farm worker wasidentified as a human carrier of S. suis.
Streptococcus suis represents apotential zoonosis risk in personnel working in close contact with swines. Thepurpose of this study was to evidence the human carrier status of S. suis in farm workers belonging tothree different pig farms located in three different cities of the state of SãoPaulo, Brazil. Cotton swab samples were taken from tonsils of 28 workers. The S. suis-like strains were identified bypolymerase chain reaction, confirmed by sequencing and serotyped bycoagglutination technique. For the first time in Brazil, one farm worker was identified as a human carrier of S. suis.