Fatal yersiniosis by Yersinia enterocolitica in a brown titi monkey (Plecturocebus brunneus) / Yersiniose fatal por Yersinia enterocolitica em um zogue-zogue (Plecturocebus brunneus)

ABSTRACT: We described a case of fatal septicemic yersiniosis in a young adult brown titi monkey (Plecturocebus brunneus) which presented lethargy and severe anemia. Postmortem external assessment revealed marked dehydration and pale pink mucous membranes. The main gross findings included enlarged liver with yellow pinpoints, enlarged spleen with yellow nodules, mucosal ulcerations in the large intestine, enlarged mesenteric lymph nodes, and pulmonary hemorrhage. Histology revealed necrosuppurative hepatosplenitis with intralesional colonies of rod-shaped gram-negative bacteria, ulcerative colitis, reactive lymphoid hyperplasia, and fibrinous and hemorrhagic pneumonia. Bacterial culture and identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry confirmed the diagnosis of yersiniosis by Yersinia enterocolitica. This study indicated that yersiniosis should be considered as a differential diagnosis of death in brown titi monkeys.

RESUMO: Descrevemos um caso de yersiniose septicêmica fatal em um zogue-zogue (Plecturocebus brunneus) jovem adulto que apresentava um quadro de letargia e anemia severa. Macroscopicamente, havia acentuada desidratação e as mucosas estavam pálidas. Notou-se hepatomegalia com múltiplos pontos amarelos e esplenomegalia com múltiplos nódulos amarelos pelo parênquima. Ainda, ulcerações da mucosa do intestino grosso, linfonodos mesentéricos aumentados e hemorragia pulmonar foram observados. A avaliação histológica revelou hepatite e esplenite necrossupurativas associadas a agregados bacterianos bacilares gram-negativos intralesionais, colite ulcerativa, hiperplasia linfoide reativa e pneumonia fibrino-hemorrágica. A cultura bacteriana e identificação através do método de espectrometria de massa por ionização e dessorção a laser assistida por matriz associada ao tempo de voo confirmou o diagnóstico de yersiniose por Yersinia enterocolitica. Este estudo demonstra que a yersiniose deve ser considerada como um diagnóstico diferencial de causa de morte em zogue-zogues.

Fatal yersiniosis by Yersinia enterocolitica in a brown titi monkey (Plecturocebus brunneus) / Yersiniose fatal por Yersinia enterocolitica em um zogue-zogue (Plecturocebus brunneus)

We described a case of fatal septicemic yersiniosis in a young adult brown titi monkey (Plecturocebus brunneus) which presented lethargy and severe anemia. Postmortem external assessment revealed marked dehydration and pale pink mucous membranes. The main gross findings included enlarged liver with yellow pinpoints, enlarged spleen with yellow nodules, mucosal ulcerations in the large intestine, enlarged mesenteric lymph nodes, and pulmonary hemorrhage. Histology revealed necrosuppurative hepatosplenitis with intralesional colonies of rod-shaped gram-negative bacteria, ulcerative colitis, reactive lymphoid hyperplasia, and fibrinous and hemorrhagic pneumonia. Bacterial culture and identification using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry confirmed the diagnosis of yersiniosis by Yersinia enterocolitica. This study indicated that yersiniosis should be considered as a differential diagnosis of death in brown titi monkeys.

Descrevemos um caso de yersiniose septicêmica fatal em um zogue-zogue (Plecturocebus brunneus) jovem adulto que apresentava um quadro de letargia e anemia severa. Macroscopicamente, havia acentuada desidratação e as mucosas estavam pálidas. Notou-se hepatomegalia com múltiplos pontos amarelos e esplenomegalia com múltiplos nódulos amarelos pelo parênquima. Ainda, ulcerações da mucosa do intestino grosso, linfonodos mesentéricos aumentados e hemorragia pulmonar foram observados. A avaliação histológica revelou hepatite e esplenite necrossupurativas associadas a agregados bacterianos bacilares gram-negativos intralesionais, colite ulcerativa, hiperplasia linfoide reativa e pneumonia fibrino-hemorrágica. A cultura bacteriana e identificação através do método de espectrometria de massa por ionização e dessorção a laser assistida por matriz associada ao tempo de voo confirmou o diagnóstico de yersiniose por Yersinia enterocolitica. Este estudo demonstra que a yersiniose deve ser considerada como um diagnóstico diferencial de causa de morte em zogue-zogues.

Cats with central nervous system cryptococcosis

Background: Cryptococcosis is a serious fungal infection contracted by humans and animals, and the most common systemic mycosis found in cats. This disease is often contracted through inhalation of fungal propagules. The Central Nervous System (CNS) may be infected through local extension (nasal and frontal sinuses) or via hematogenous route. Similarly to CNS bacterial infection, the clinical signs of neurological dysfunction may be attributed to mass effect (gelatinous mass of fungal microorganisms and fungal granuloma formation) or to a more disseminated inflammatory response to invading microorganisms. The objective of this study is to report one case of a patient with cryptococcal granulomas in the central nervous system and one case of a patient with neurological signs associated to a cryptococcosis. Cases: Case 1. A 3-year-old male mixed breed feline was admitted to a veterinary clinic, located in Porto Alegre, RS, Southern Brazil. The patient presented unsourced behavioral changes, vestibular ataxia and dysphagia caused by inability of coordination. The following tests were performed: complete blood count test, biochemical analysis, computed tomography scan (CT scan), fluid analysis, radiography and toxoplasmosis test. The following medicine were administrated for treatment: fluconazole, dexamethasone, mannitol, phenobarbital and levetiracetam. Fluid therapy was also part of the treatment. Immediately after death, the cat was submitted for necropsy, and a fungal granulomatous meningoencephalomyelitis was diagnosed. Cryptococcus sp. was identified as the causal agent through pathological findings, fungal culture and PCR analysis. Case 2. One year later, another feline was admitted to the same clinic (a 2-year-old female mixed breed) presenting hypersalivation, tremors and excessive vocalization. The patient had contact with the deceased feline. The following tests were performed: complete blood count...

Cats with central nervous system cryptococcosis

Background: Cryptococcosis is a serious fungal infection contracted by humans and animals, and the most common systemic mycosis found in cats. This disease is often contracted through inhalation of fungal propagules. The Central Nervous System (CNS) may be infected through local extension (nasal and frontal sinuses) or via hematogenous route. Similarly to CNS bacterial infection, the clinical signs of neurological dysfunction may be attributed to mass effect (gelatinous mass of fungal microorganisms and fungal granuloma formation) or to a more disseminated inflammatory response to invading microorganisms. The objective of this study is to report one case of a patient with cryptococcal granulomas in the central nervous system and one case of a patient with neurological signs associated to a cryptococcosis. Cases: Case 1. A 3-year-old male mixed breed feline was admitted to a veterinary clinic, located in Porto Alegre, RS, Southern Brazil. The patient presented unsourced behavioral changes, vestibular ataxia and dysphagia caused by inability of coordination. The following tests were performed: complete blood count test, biochemical analysis, computed tomography scan (CT scan), fluid analysis, radiography and toxoplasmosis test. The following medicine were administrated for treatment: fluconazole, dexamethasone, mannitol, phenobarbital and levetiracetam. Fluid therapy was also part of the treatment. Immediately after death, the cat was submitted for necropsy, and a fungal granulomatous meningoencephalomyelitis was diagnosed. Cryptococcus sp. was identified as the causal agent through pathological findings, fungal culture and PCR analysis. Case 2. One year later, another feline was admitted to the same clinic (a 2-year-old female mixed breed) presenting hypersalivation, tremors and excessive vocalization. The patient had contact with the deceased feline. The following tests were performed: complete blood count...(AU)

Aspectos anatomopatológicos e imuno-histoquímicos de cinomose em graxains / Anatomopathological and immunohistochemical aspects of distemper virus in crab-eating-foxes and pampa-foxes

Background: Distemper is a contagious disease with worldwide distribution, which is caused by a single-stranded RNAvirus of the genus Morbillivirus. The Crab-eating fox (Cerdocyon thous) and the Pampas fox (Lycalopex gymnocercus), wildcanids commonly found in the state of Rio Grande do Sul, are highly susceptible to the disease. Distemper virus infection oftenshows a multisystemic presentation. Central nervous system lesions are frequently reported, and may lead to death of wild canids.Distemper virus infection affecting these species has been cited by several studies; nonetheless, case descriptions involving theCrab-eating fox and the Pampas fox are infrequent. Therefore, the objective of the present study is to describe the anatomopathological and immunohistochemical aspects of distemper in the referred species, aiming to assist with the diagnosis of the disease.Materials, Methods & Results: A retrospective study was performed, and the necropsy reports of Crab-eating foxes and Pampasfoxes admitted to SPV-UFRGS from 2010 to 2016 were reviewed. Necropsy reports were revised in order to obtain information related to the clinical history and anatomopathological findings, and cases compatible with distemper were selected. Dataregarding the affected species, sex, approximate age, origin and clinical signs were compiled and analyzed. Selected histologicalsections of brain and cerebellum were submitted for immunohistochemistry (IHC) for monoclonal antibody canine distempervirus. In the studied period, 20 animals of the referred species were subjected to necropsy, of which four were diagnosed withdistemper as a cause of death. Of the affected animals, two were female and two were male. Three were juvenile and one wasan adult animal. Clinical signs reported included myoclonia, opisthotonos, forelimb paresis and nystagmus. No significant grosschanges were noted. Histopathological findings were restricted to the central nervous system...

Aspectos epidemiológicos e patológicos do complexo granuloma eosinofílico felino / Epidemiologic and pathologic aspects of feline eosinophilic granuloma complex

Background: The feline eosinophilic granuloma complex (FEGC) includes a group of lesions that affect the skin, mucocutaneous junctions, and oral cavity of cats. It comprises three distinct clinical entities: the eosinophilic ulcer (EU), theeosinophilic plaque (EP) and the eosinophilic granuloma (EG). The EU is usually found in the upper lip. Lesions of EPoccur most commonly on the abdomen and medial thigh. The EG lesions usually appear on the tongue and hard palate.The aim of this study is to describe the localization and pathological findings of FEGC through a retrospective study andtesting by immunohistochemistry if feline herpesvirus type 1 (FHV-1) infection may be associated.Materials, Methods & Results: The records of biopsy specimens from the Department of Veterinary Pathology of theUniversidade Federal do Rio Grande do Sul were recovered and cats diagnosed with FEGC were selected since January2006 to December 2017. General data, such as age, sex, breed and distribution lesions, were analyzed and compiled. Thehistological slides were reviewed by optical microscopy and the microscopic findings were detailed. Sections of tissuewere used in immunohistochemistry to investigate the presence of feline herpesvirus-1 (FHV-1) using anti-FHV-1 antibody. In this study, 30 cats were diagnosed with FEGC. Most part of the cats were mixed breed (27/30), and there wereno sex predisposition. The age of the cats ranged from seven months to 13 years old, and the median was three years. Thelesion distribution of FEGC was 40% in oral cavity, 33.3% in skin and 26.6% in mucocutaneous junction. Histologically,the main lesion of all distributions was characterized by diffuse dermal/submucosa inflammatory infiltrate composed ofeosinophils. Within the inflammation there were large irregular foci of collagen...

Aspectos anatomopatológicos e imuno-histoquímicos de cinomose em graxains / Anatomopathological and immunohistochemical aspects of distemper virus in crab-eating-foxes and pampa-foxes

Background: Distemper is a contagious disease with worldwide distribution, which is caused by a single-stranded RNAvirus of the genus Morbillivirus. The Crab-eating fox (Cerdocyon thous) and the Pampas fox (Lycalopex gymnocercus), wildcanids commonly found in the state of Rio Grande do Sul, are highly susceptible to the disease. Distemper virus infection oftenshows a multisystemic presentation. Central nervous system lesions are frequently reported, and may lead to death of wild canids.Distemper virus infection affecting these species has been cited by several studies; nonetheless, case descriptions involving theCrab-eating fox and the Pampas fox are infrequent. Therefore, the objective of the present study is to describe the anatomopathological and immunohistochemical aspects of distemper in the referred species, aiming to assist with the diagnosis of the disease.Materials, Methods & Results: A retrospective study was performed, and the necropsy reports of Crab-eating foxes and Pampasfoxes admitted to SPV-UFRGS from 2010 to 2016 were reviewed. Necropsy reports were revised in order to obtain information related to the clinical history and anatomopathological findings, and cases compatible with distemper were selected. Dataregarding the affected species, sex, approximate age, origin and clinical signs were compiled and analyzed. Selected histologicalsections of brain and cerebellum were submitted for immunohistochemistry (IHC) for monoclonal antibody canine distempervirus. In the studied period, 20 animals of the referred species were subjected to necropsy, of which four were diagnosed withdistemper as a cause of death. Of the affected animals, two were female and two were male. Three were juvenile and one wasan adult animal. Clinical signs reported included myoclonia, opisthotonos, forelimb paresis and nystagmus. No significant grosschanges were noted. Histopathological findings were restricted to the central nervous system...(AU)

Aspectos epidemiológicos e patológicos do complexo granuloma eosinofílico felino / Epidemiologic and pathologic aspects of feline eosinophilic granuloma complex

Background: The feline eosinophilic granuloma complex (FEGC) includes a group of lesions that affect the skin, mucocutaneous junctions, and oral cavity of cats. It comprises three distinct clinical entities: the eosinophilic ulcer (EU), theeosinophilic plaque (EP) and the eosinophilic granuloma (EG). The EU is usually found in the upper lip. Lesions of EPoccur most commonly on the abdomen and medial thigh. The EG lesions usually appear on the tongue and hard palate.The aim of this study is to describe the localization and pathological findings of FEGC through a retrospective study andtesting by immunohistochemistry if feline herpesvirus type 1 (FHV-1) infection may be associated.Materials, Methods & Results: The records of biopsy specimens from the Department of Veterinary Pathology of theUniversidade Federal do Rio Grande do Sul were recovered and cats diagnosed with FEGC were selected since January2006 to December 2017. General data, such as age, sex, breed and distribution lesions, were analyzed and compiled. Thehistological slides were reviewed by optical microscopy and the microscopic findings were detailed. Sections of tissuewere used in immunohistochemistry to investigate the presence of feline herpesvirus-1 (FHV-1) using anti-FHV-1 antibody. In this study, 30 cats were diagnosed with FEGC. Most part of the cats were mixed breed (27/30), and there wereno sex predisposition. The age of the cats ranged from seven months to 13 years old, and the median was three years. Thelesion distribution of FEGC was 40% in oral cavity, 33.3% in skin and 26.6% in mucocutaneous junction. Histologically,the main lesion of all distributions was characterized by diffuse dermal/submucosa inflammatory infiltrate composed ofeosinophils. Within the inflammation there were large irregular foci of collagen...(AU)