Arterial thromboembolism secondary to subaortic stenosis in a persian kitten

Background: Aortic stenosis refers to several types of anatomic and functional obstructions of the left ventricular outflow tract. In small animals, this anomaly is most commonly documented as either a congenital lesion or an obstruction that develops soon after birth. It is a hereditary disease, caused by a dominant autosomal gene and modifying genes that interfere with phenotype expression. Even though aortic thromboembolism may be a potential complication in cats with cardiac diseases, aortic stenosis is deemed rare in that species. In this paper, we report an unusual case of a Persian kitten with aortic stenosis that eventually developed arterial thromboembolism.Case: A 7-month-old Persian kitten (3.1 kg) was admitted to a Veterinary Teaching Hospital with a history of hindlimbs paralysis over the past 48 h, as well as excessive vocalization. Also, the cat presented with inappetence, adipsia, urine incontinence, and hematuria. On physical examination, we observed hypothermia and cold paws. The footpads were cyanotic and there were no palpable femoral pulses. Cardiac auscultation disclosed a regular fast pace (200 bpm) and a grade 2/6 murmur heard best over the left cardiac base, but irradiating to the contralateral hemithorax. Prothrombin time and activated partial thromboplastin time were within the normal reference range, but the complete blood count showed microcytosis, lymphopenia, and hyperproteinemia. Also, elevated alanine aminotransferase and BUN were identified. On the echocardiogram, we observed a subvalvular aortic stenosis, which resulted in concentric remodeling of the left ventricle and a mild left atrial dilation. The stenotic lesion was classified as mild. Also, the thoracic radiography unveiled cardiomegaly. The recommended therapy included atenolol(6.25 mg/cat PO, q24h), clopidogrel (18.7 mg/cat PO, q24h), enoxaparin (1 mg/kg SC, q24 h), methadone (0.2 mg/kg IM, q8h) and amoxicillin + potassium clavulanate (20 mg/kg SC, q12h).[...]

Arterial thromboembolism secondary to subaortic stenosis in a persian kitten

Background: Aortic stenosis refers to several types of anatomic and functional obstructions of the left ventricular outflow tract. In small animals, this anomaly is most commonly documented as either a congenital lesion or an obstruction that develops soon after birth. It is a hereditary disease, caused by a dominant autosomal gene and modifying genes that interfere with phenotype expression. Even though aortic thromboembolism may be a potential complication in cats with cardiac diseases, aortic stenosis is deemed rare in that species. In this paper, we report an unusual case of a Persian kitten with aortic stenosis that eventually developed arterial thromboembolism.Case: A 7-month-old Persian kitten (3.1 kg) was admitted to a Veterinary Teaching Hospital with a history of hindlimbs paralysis over the past 48 h, as well as excessive vocalization. Also, the cat presented with inappetence, adipsia, urine incontinence, and hematuria. On physical examination, we observed hypothermia and cold paws. The footpads were cyanotic and there were no palpable femoral pulses. Cardiac auscultation disclosed a regular fast pace (200 bpm) and a grade 2/6 murmur heard best over the left cardiac base, but irradiating to the contralateral hemithorax. Prothrombin time and activated partial thromboplastin time were within the normal reference range, but the complete blood count showed microcytosis, lymphopenia, and hyperproteinemia. Also, elevated alanine aminotransferase and BUN were identified. On the echocardiogram, we observed a subvalvular aortic stenosis, which resulted in concentric remodeling of the left ventricle and a mild left atrial dilation. The stenotic lesion was classified as mild. Also, the thoracic radiography unveiled cardiomegaly. The recommended therapy included atenolol(6.25 mg/cat PO, q24h), clopidogrel (18.7 mg/cat PO, q24h), enoxaparin (1 mg/kg SC, q24 h), methadone (0.2 mg/kg IM, q8h) and amoxicillin + potassium clavulanate (20 mg/kg SC, q12h).[...](AU)

First occurrence of blaOXA-58 in Acinetobacter baumannii isolated from a clinical sample in Southern Brazil

This is the first report of an Acinetobacter baumannii from clinical origin carrying the blaOXA-58 gene in Brazil. The isolate included in this study was from a patient during an outbreak in Porto Alegre, RS, Southern Brazil, in 2007. It was resistant to most of the beta-lactams tested, it has also the blaOXA-65 gene and the ISAba1 sequence located upstream to both blaOXA genes detected and it has a MIC of imipenem of 64 g/mL.

Spread of Metallo--lactamases: screening reveals the presence of a BLA SPM-1 gene in hospital sewage in southern Brazil

Of 396 Pseudomonas aeruginosa strains isolated from hospital sewage, the blaSPM-1 gene was confirmed in nine. This is the first report of environmental P. aeruginosa strains carrying the blaSPM-1 gene in Brazil. The carbapenem resistance, already disseminated among clinical isolates, has been detected among environmental isolates.

Ao todo, 396 isolados de Pseudomonas aeruginosa foram estudados. O gene blaSPM-1 foi encontrado em nove isolados de efluente hospitalar. Este estudo é o primeiro relato de isolados ambientais de P. aeruginosa com o gene blaSPM-1no Brasil. A resistência aos carbapenêmicos, amplamente disseminada entre isolados clínicos, já é detectada em isolados ambientais.

Emergency thoracotomy for massive hemothorax

A tree year old female Cocker Spaniel was admitted, hitted by a car. The patient presented progressive hypotension, epistaxis, fast and weak pulse, capillary refill time (CRT) of 4 seconds and progressive dispnea. An infusion with lactated ringer (90ml/kg/h IV) and oxygen therapy were started. The thoracocentesis revealed massive hemopneumothorax in both sides of the chest, and one thoracic drain was inserted in each hemithorax. Peripherical pulse became untouchable indicating severe blood pressure fall, and the patient got unconscious. In this way, was decided to perform an emergency thoracotomy by a transesternal resection on the fifth intercostal space, and a pulmonary lobectomy was than executed. The patient had a good recovery and at this moment she is healthy.

Typing of avian pathogenic Escherichia coli strains by REP-PCR / Estratégias imunoprofiláticas contra enterotoxemia causada por Clostridium perfringens tipo D em caprinos

A técnica de REP (Repetitive extragenic palindrome)-PCR foi utilizada para avaliar a variabilidade genética de 49 amostras de Escherichia coli patogênicas para aves (APEC), isoladas de aves de corte (frangos) em diferentes surtos de septicemia (n=24), síndrome da cabeça inchada (n=14) e onfalite (n=11). Trinta amostras comensais, isoladas de frangos sem sinais de doença, foram utilizadas como controle. A análise do perfil eletroforético obtido por reação de REP-PCR utilizando DNA purificado das amostras evidenciou a amplificação de 0 a 15 bandas de DNA com pesos moleculares variando entre 100 pb e 6.1 Kb. A análise deste padrão permitiu a construção de um dendrograma demonstrando o agrupamento das 79 amostras em 49 perfis distintos. Embora a técnica de REP-PCR tenha apresentado grande poder discriminatório, as amostras patogênicas e não patogênicas não foram discriminadas entre si assim como não foi observado o agrupamento de amostras causadoras do mesmo tipo de doença. Por outro lado, demonstramos recentemente que outras técnicas tais como ERIC-PCR e a análise de isoenzimas foram eficientes quando utilizadas para esta mesma finalidade. Concluindo, REP-PCR parece não ser uma técnica eficiente e universal para discriminar entre amostras APEC. Porém, a estrutura clonal populacional obtida com o uso de REP-PCR não deve ser desprezada, particularmente se considerarmos que os mecanismos de patogenicidade de APEC ainda não são completamente conhecidos.(AU)

In the present study the repetitive extragenic palindromic (REP) polymerase chain reaction (PCR) technique was used to establish the clonal variability of 49 avian Escherichia coli (APEC) strains isolated from different outbreak cases of septicemia (n=24), swollen head syndrome (n=14) and omphalitis (n=11). Thirty commensal strains isolated from poultry with no signs of these illnesses were used as control strains. The purified DNA of these strains produced electrophoretic profiles ranging from 0 to 15 bands with molecular sizes varying from 100 bp to 6.1 kb, allowing the grouping of the 79 strains into a dendrogram containing 49 REP-types. Although REP-PCR showed good discriminating power it was not able to group the strains either into specific pathogenic classes or to differentiate between pathogenic and non-pathogenic strains. On the contrary, we recently demonstrated that other techniques such as ERIC-PCR and isoenzyme profiles are appropriate to discriminate between commensal and APEC strains and also to group these strains into specific pathogenic classes. In conclusion, REP-PCR seems to be a technique neither efficient nor universal for APEC strains discrimination. However, the population clonal structure obtained with the use of REP-PCR must not be ignored particularly if one takes into account that the APEC pathogenic mechanisms are not completely understood yet.(AU)

Beating heart partial ventriculectomy in dogs

After valve endocardiosis, dilated cardiomyopathy is the most diagnosed cardiac affection in dogs. Sometimes this disease is unresponsible to medical therapy, in this way surgery becomes necessary to its correction. Unfortunately, dogs do not support cardiopulmonary bypass because its tendency in microtrombus formation in the capillary vessels of the lung circulation. Due to this condition, another surgical alternatives were developed, in order to correct the increase in ventricle size, as the ventricle plication or partial ventriculectomy with the inflow occlusion. However, this kind of technique has its complications, as well the ventricle plication. In plication, a part of necrotic heart tissue still remains, and if the necrosis does not happen, that portion of myocardium keeps its oxygen consumption, leading to heart failure. The partial ventriculectomy with the inflow occlusion is time dependent. For its execution it means that the surgeon gets only four minutes to perform the resection of the dilated ventricle and then suture the remaining defect after the inflow occlusion. The aim of this study is to develop a new technique of heart size reduction, called by the authors Beating Heart Partial Ventriculectomy.

Beating heart partial ventriculectomy in dogs

After valve endocardiosis, dilated cardiomyopathy is the most diagnosed cardiac affection in dogs. Sometimes this disease is unresponsible to medical therapy, in this way surgery becomes necessary to its correction. Unfortunately, dogs do not support cardiopulmonary bypass because its tendency in microtrombus formation in the capillary vessels of the lung circulation. Due to this condition, another surgical alternatives were developed, in order to correct the increase in ventricle size, as the ventricle plication or partial ventriculectomy with the inflow occlusion. However, this kind of technique has its complications, as well the ventricle plication. In plication, a part of necrotic heart tissue still remains, and if the necrosis does not happen, that portion of myocardium keeps its oxygen consumption, leading to heart failure. The partial ventriculectomy with the inflow occlusion is time dependent. For its execution it means that the surgeon gets only four minutes to perform the resection of the dilated ventricle and then suture the remaining defect after the inflow occlusion. The aim of this study is to develop a new technique of heart size reduction, called by the authors Beating Heart Partial Ventriculectomy.

Avaliação moroflógica e estereológica comparativa do implante da tela de polipropileno e peritônio de pacas (Cuniculus paca, L. 1766) na correção de defeitos da parede abdominal de ratos Wistar

Na busca de material biológico alternativo para a realização de implantes, objetivou-se com o presente estudo avaliar comparativamente a implantação do peritônio da paca conservado em glicerina a 98%, uma nova opção de biomaterial e a tela de polipropileno, consagrada prótese sintética na parede abdominal de ratos Wistar. Foram utilizados 60 ratos, machos, da linhagem Wistar pesando entre 150 e 200 gramas organizados nos seguintes grupos experimentais: grupo controle (GI), grupo peritônio conservado em glicerina a 98% (GII) e grupo tela de polipropileno (GIII), cada um com 20 animais. O grupo GII recebeu o enxerto de peritônio da paca conservado em glicerina 98%, o grupo GIII recebeu o enxerto da tela de polipropileno e o grupo GI não recebeu a membrana. Cinco ratos de cada grupo foram submetidos à eutanásia em quatro momentos distintos: sete, 15, 30 e 60 dias de pós-operatórios para avaliações macroscópicas e microscópicas da interface implante-tecido nativo. Em 100% dos animais do grupo GII e GIII houve boa cicatrização da membrana. Na análise histológica, nos animais que receberam o peritônio da paca preservado em glicerina a 98% ficou demonstrada reação inflamatória aguda (M7 e M15), que gradativamente foi desaparecendo e a membrana foi sendo substituída por tecido conjuntivo fibroso (M30 e M60). Nos animais do GIII que receberam a tela de polipropileno verificou-se infiltrado inflamatório com predominância de mononucleares (M7, M15, M30 e M60), que gradativamente foi desaparecendo e a membrana foi sendo substituída por tecido conjuntivo fibroso (M30 e M60). Pode-se concluir que o peritônio da paca conservado em glicerina e a tela de polipropileno, promoveram reforço abdominal satisfatório no reparo de defeito produzido na parede abdominal de ratos

In search of alternative biological material to perform implants, this study aimed to compare the implantation of paca peritoneum preserved in glycerin 98% a new option of biomaterial and polypropylene mesh , consecrated synthetic prosthesis in the abdominal wall of wistar rats. A total of 60 male rats of wistar strain weighting between 150 and 200 grams housed into three diferent experimental groups: control group (GI), peritoneum preserved in glycerin 98% (GII) and polypropylene mesh group, with 20 animals each one. The group GII received the paca peritoneum graft preserved in glycerin 98%, the group GIII received the polypropylene mesh and the group GI did not receive any membrane. Five rats from each group were euthanized at four different times: seven, 15, 30 and 60 days postsurgery to macroscopic and microscopic evaluations in graft-native tissue interface. 100% of the animals of the GII and GIII had good healing of the membrane. On histological examination, the animals that had received the paca peritoneum graft preserved in glycerin 98% , showed inflammatory infiltrates (M7 and M15), wich gradually disappeared and the membrane was replaced by connective tissue (M30 e M60). The animals that received the polypropylene mesh had inflammatory infiltrates with predominance of mononucleares (M7, M15, M30 and M60), wich gradually disappeared and the membrane was being replaced by connective tissue (M30 e M60). It was concluded that the paca peritoneum conserved in glycerin 98% and the polypropylene mesh promote strengthening abdominal quite satisfying in repair induced defect in the abdominal wall of rats

Emergency thoracotomy for massive hemothorax

A tree year old female Cocker Spaniel was admitted, hitted by a car. The patient presented progressive hypotension, epistaxis, fast and weak pulse, capillary refill time (CRT) of 4 seconds and progressive dispnea. An infusion with lactated ringer (90ml/kg/h IV) and oxygen therapy were started. The thoracocentesis revealed massive hemopneumothorax in both sides of the chest, and one thoracic drain was inserted in each hemithorax. Peripherical pulse became untouchable indicating severe blood pressure fall, and the patient got unconscious. In this way, was decided to perform an emergency thoracotomy by a transesternal resection on the fifth intercostal space, and a pulmonary lobectomy was than executed. The patient had a good recovery and at this moment she is healthy.

Emergency thoracotomy for massive hemothorax

A tree year old female Cocker Spaniel was admitted, hitted by a car. The patient presented progressive hypotension, epistaxis, fast and weak pulse, capillary refill time (CRT) of 4 seconds and progressive dispnea. An infusion with lactated ringer (90ml/kg/h IV) and oxygen therapy were started. The thoracocentesis revealed massive hemopneumothorax in both sides of the chest, and one thoracic drain was inserted in each hemithorax. Peripherical pulse became untouchable indicating severe blood pressure fall, and the patient got unconscious. In this way, was decided to perform an emergency thoracotomy by a transesternal resection on the fifth intercostal space, and a pulmonary lobectomy was than executed. The patient had a good recovery and at this moment she is healthy.

Emergency thoracotomy for massive hemothorax

A tree year old female Cocker Spaniel was admitted, hitted by a car. The patient presented progressive hypotension, epistaxis, fast and weak pulse, capillary refill time (CRT) of 4 seconds and progressive dispnea. An infusion with lactated ringer (90ml/kg/h IV) and oxygen therapy were started. The thoracocentesis revealed massive hemopneumothorax in both sides of the chest, and one thoracic drain was inserted in each hemithorax. Peripherical pulse became untouchable indicating severe blood pressure fall, and the patient got unconscious. In this way, was decided to perform an emergency thoracotomy by a transesternal resection on the fifth intercostal space, and a pulmonary lobectomy was than executed. The patient had a good recovery and at this moment she is healthy.

Emergency thoracotomy for massive hemothorax

A tree year old female Cocker Spaniel was admitted, hitted by a car. The patient presented progressive hypotension, epistaxis, fast and weak pulse, capillary refill time (CRT) of 4 seconds and progressive dispnea. An infusion with lactated ringer (90ml/kg/h IV) and oxygen therapy were started. The thoracocentesis revealed massive hemopneumothorax in both sides of the chest, and one thoracic drain was inserted in each hemithorax. Peripherical pulse became untouchable indicating severe blood pressure fall, and the patient got unconscious. In this way, was decided to perform an emergency thoracotomy by a transesternal resection on the fifth intercostal space, and a pulmonary lobectomy was than executed. The patient had a good recovery and at this moment she is healthy.

Emergency thoracotomy for massive hemothorax

A tree year old female Cocker Spaniel was admitted, hitted by a car. The patient presented progressive hypotension, epistaxis, fast and weak pulse, capillary refill time (CRT) of 4 seconds and progressive dispnea. An infusion with lactated ringer (90ml/kg/h IV) and oxygen therapy were started. The thoracocentesis revealed massive hemopneumothorax in both sides of the chest, and one thoracic drain was inserted in each hemithorax. Peripherical pulse became untouchable indicating severe blood pressure fall, and the patient got unconscious. In this way, was decided to perform an emergency thoracotomy by a transesternal resection on the fifth intercostal space, and a pulmonary lobectomy was than executed. The patient had a good recovery and at this moment she is healthy.

Beating heart partial ventriculectomy in dogs

After valve endocardiosis, dilated cardiomyopathy is the most diagnosed cardiac affection in dogs. Sometimes this disease is unresponsible to medical therapy, in this way surgery becomes necessary to its correction. Unfortunately, dogs do not support cardiopulmonary bypass because its tendency in microtrombus formation in the capillary vessels of the lung circulation. Due to this condition, another surgical alternatives were developed, in order to correct the increase in ventricle size, as the ventricle plication or partial ventriculectomy with the inflow occlusion. However, this kind of technique has its complications, as well the ventricle plication. In plication, a part of necrotic heart tissue still remains, and if the necrosis does not happen, that portion of myocardium keeps its oxygen consumption, leading to heart failure. The partial ventriculectomy with the inflow occlusion is time dependent. For its execution it means that the surgeon gets only four minutes to perform the resection of the dilated ventricle and then suture the remaining defect after the inflow occlusion. The aim of this study is to develop a new technique of heart size reduction, called by the authors Beating Heart Partial Ventriculectomy.

Beating heart partial ventriculectomy in dogs

After valve endocardiosis, dilated cardiomyopathy is the most diagnosed cardiac affection in dogs. Sometimes this disease is unresponsible to medical therapy, in this way surgery becomes necessary to its correction. Unfortunately, dogs do not support cardiopulmonary bypass because its tendency in microtrombus formation in the capillary vessels of the lung circulation. Due to this condition, another surgical alternatives were developed, in order to correct the increase in ventricle size, as the ventricle plication or partial ventriculectomy with the inflow occlusion. However, this kind of technique has its complications, as well the ventricle plication. In plication, a part of necrotic heart tissue still remains, and if the necrosis does not happen, that portion of myocardium keeps its oxygen consumption, leading to heart failure. The partial ventriculectomy with the inflow occlusion is time dependent. For its execution it means that the surgeon gets only four minutes to perform the resection of the dilated ventricle and then suture the remaining defect after the inflow occlusion. The aim of this study is to develop a new technique of heart size reduction, called by the authors Beating Heart Partial Ventriculectomy.

Beating heart partial ventriculectomy in dogs

After valve endocardiosis, dilated cardiomyopathy is the most diagnosed cardiac affection in dogs. Sometimes this disease is unresponsible to medical therapy, in this way surgery becomes necessary to its correction. Unfortunately, dogs do not support cardiopulmonary bypass because its tendency in microtrombus formation in the capillary vessels of the lung circulation. Due to this condition, another surgical alternatives were developed, in order to correct the increase in ventricle size, as the ventricle plication or partial ventriculectomy with the inflow occlusion. However, this kind of technique has its complications, as well the ventricle plication. In plication, a part of necrotic heart tissue still remains, and if the necrosis does not happen, that portion of myocardium keeps its oxygen consumption, leading to heart failure. The partial ventriculectomy with the inflow occlusion is time dependent. For its execution it means that the surgeon gets only four minutes to perform the resection of the dilated ventricle and then suture the remaining defect after the inflow occlusion. The aim of this study is to develop a new technique of heart size reduction, called by the authors Beating Heart Partial Ventriculectomy.

Beating heart partial ventriculectomy in dogs

After valve endocardiosis, dilated cardiomyopathy is the most diagnosed cardiac affection in dogs. Sometimes this disease is unresponsible to medical therapy, in this way surgery becomes necessary to its correction. Unfortunately, dogs do not support cardiopulmonary bypass because its tendency in microtrombus formation in the capillary vessels of the lung circulation. Due to this condition, another surgical alternatives were developed, in order to correct the increase in ventricle size, as the ventricle plication or partial ventriculectomy with the inflow occlusion. However, this kind of technique has its complications, as well the ventricle plication. In plication, a part of necrotic heart tissue still remains, and if the necrosis does not happen, that portion of myocardium keeps its oxygen consumption, leading to heart failure. The partial ventriculectomy with the inflow occlusion is time dependent. For its execution it means that the surgeon gets only four minutes to perform the resection of the dilated ventricle and then suture the remaining defect after the inflow occlusion. The aim of this study is to develop a new technique of heart size reduction, called by the authors Beating Heart Partial Ventriculectomy.