Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 14 de 14
Filtrar
Mais filtros

Base de dados
Intervalo de ano de publicação
1.
Anim. Reprod. (Online) ; 19(1): e20210083, 2022. tab, graf
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1360099

Resumo

Abstract The action of substances with non-permeable cryoprotectant potential, besides glucose, has not yet been studied for the species Prochilodus brevis. The objective of this work was to evaluate the action of four non-permeable cryoprotectants on this species sperm cryopreservation. Five pools were cryopreserved in a solution of 5% glucose and 10% dimethyl sulfoxide (Me2SO) associated or not (control) with cryoprotectants egg yolk (5, 10 or 12%), soy lecithin (2.5, 7.5 or 10%), sucrose (5, 10 or 20%) and lactose (5, 8 or 15%). After thawing, samples were evaluated for sperm kinetics (total motility, motility duration, velocities, and wobble - WOB), morphology and membrane and DNA integrity. The treatments containing egg yolk improved significantly (P<0.05) results when compared the control for the membrane integrity parameter. When compared to other treatments, egg yolk, at any concentration, presented higher results (P<0.05) for membrane integrity, total motility, curvilinear velocity (VCL) and average path velocity (VAP) parameters. Egg yolk also showed the best results for WOB, but it did not differ from 5% and 8% lactose and 5% and 20% sucrose. Soy lecithin had the lowest percentages of morphologically normal sperm (P<0.05), while the other treatments did not differ from each other. There was no difference regarding DNA integrity data. Thus, 5% egg yolk is indicated as a non-permeable cryoprotectant for P. brevis, in association with 5% glucose and 10% Me2SO.

2.
Anim. Reprod. ; 19(1): e20210083, 2022. tab, graf
Artigo em Inglês | VETINDEX | ID: vti-765785

Resumo

The action of substances with non-permeable cryoprotectant potential, besides glucose, has not yet been studied for the species Prochilodus brevis. The objective of this work was to evaluate the action of four non-permeable cryoprotectants on this species sperm cryopreservation. Five pools were cryopreserved in a solution of 5% glucose and 10% dimethyl sulfoxide (Me2SO) associated or not (control) with cryoprotectants egg yolk (5, 10 or 12%), soy lecithin (2.5, 7.5 or 10%), sucrose (5, 10 or 20%) and lactose (5, 8 or 15%). After thawing, samples were evaluated for sperm kinetics (total motility, motility duration, velocities, and wobble - WOB), morphology and membrane and DNA integrity. The treatments containing egg yolk improved significantly (P<0.05) results when compared the control for the membrane integrity parameter. When compared to other treatments, egg yolk, at any concentration, presented higher results (P<0.05) for membrane integrity, total motility, curvilinear velocity (VCL) and average path velocity (VAP) parameters. Egg yolk also showed the best results for WOB, but it did not differ from 5% and 8% lactose and 5% and 20% sucrose. Soy lecithin had the lowest percentages of morphologically normal sperm (P<0.05), while the other treatments did not differ from each other. There was no difference regarding DNA integrity data. Thus, 5% egg yolk is indicated as a non-permeable cryoprotectant for P. brevis, in association with 5% glucose and 10% Me2SO.(AU)


Assuntos
Animais , Peixes , Criopreservação , Characidae , Crioprotetores
3.
Ciênc. Anim. (Impr.) ; 30(04, Supl. 2): 138-147, 2020. ilus
Artigo em Português | VETINDEX | ID: biblio-1472551

Resumo

A criopreservação seminal é uma das biotécnica reprodutivas mais promissoras para a aquicultura, pois permite o transporte de gametas, garante melhorias genéticas em espécies desejadas e minimiza a assincronia entre machos e fêmeas reprodutores. Apesar dos benefícios, danos celulares podem ser ocasionados durante o processo de congelação, causando a diminuição da qualidade dos espermatozoides. Sendo assim, estudos que busquem alternativas que minimizem tais danos são de grande importância. As proteínas anticongelantes (AFPs) são importantes compostos originados a partir de processos evolutivos em organismos que vivem em ambientes polares. As AFPs podem ser utilizadas como possíveis agentes crioprotetores, pois são capazes de diminuir os cristais de gelo e proteger a membrana plasmática das células durante o processo de congelação. Dessa forma, esta revisão tem como objetivo reunir informações acerca da utilização de proteínas anticongelantes na conservação do sêmen de peixes e apresentar os principais resultados encontrados na literatura. Observou-se que a utilização dessas substâncias na criopreservação seminal em peixes promoveu melhorias nas taxas de motilidade espermática, velocidades e integridade de membrana plasmática. Tais melhorias foram observadas de maneira interespecífica. Portanto, ainda são necessários estudos mais aprofundados com intuito de verificar o efeito das AFPs na capacidade de fertilização, buscando a melhor opção para suplementação do meio.


Seminal cryopreservation is one of the most promising reproductive biotechniques for aquaculture, as it allows the transport of gametes, ensures genetic improvements in desired species and minimizes asynchrony between breeding males and females. Despite the benefits, cell damage can be caused during the freezing process, causing decreased quality of sperm. Therefore, studies that seek alternatives that minimize such damage are of great importance. Antifreeze proteins (AFPs) are important compounds originated from evolutionary processes in organisms that live in polar environments. AFPs can be used as possible cryoprotective agents, as they are able to decrease ice crystals and protect the plasma membrane of cells during the freezing process. Thus, this review aims to gather information about the use of antifreeze proteins in the conservation of fish semen and present the main results found in the literature. It was observed that the use of these substances in seminal cryopreservation in fish promoted improvements in the rates of sperm motility, velocities and integrity of the plasma membrane. Such improvements were observed in an interspecific manner. Therefore, further studies are needed in order to verify the effect of AFPs on fertilization capacity, seeking the best option for supplementing the medium.


Assuntos
Masculino , Animais , Criopreservação/métodos , Criopreservação/veterinária , Espermatozoides , Peixes/genética , Reprodução/genética , Sêmen
4.
Ci. Anim. ; 30(04, Supl. 2): 138-147, 2020. ilus
Artigo em Português | VETINDEX | ID: vti-32216

Resumo

A criopreservação seminal é uma das biotécnica reprodutivas mais promissoras para a aquicultura, pois permite o transporte de gametas, garante melhorias genéticas em espécies desejadas e minimiza a assincronia entre machos e fêmeas reprodutores. Apesar dos benefícios, danos celulares podem ser ocasionados durante o processo de congelação, causando a diminuição da qualidade dos espermatozoides. Sendo assim, estudos que busquem alternativas que minimizem tais danos são de grande importância. As proteínas anticongelantes (AFPs) são importantes compostos originados a partir de processos evolutivos em organismos que vivem em ambientes polares. As AFPs podem ser utilizadas como possíveis agentes crioprotetores, pois são capazes de diminuir os cristais de gelo e proteger a membrana plasmática das células durante o processo de congelação. Dessa forma, esta revisão tem como objetivo reunir informações acerca da utilização de proteínas anticongelantes na conservação do sêmen de peixes e apresentar os principais resultados encontrados na literatura. Observou-se que a utilização dessas substâncias na criopreservação seminal em peixes promoveu melhorias nas taxas de motilidade espermática, velocidades e integridade de membrana plasmática. Tais melhorias foram observadas de maneira interespecífica. Portanto, ainda são necessários estudos mais aprofundados com intuito de verificar o efeito das AFPs na capacidade de fertilização, buscando a melhor opção para suplementação do meio.(AU)


Seminal cryopreservation is one of the most promising reproductive biotechniques for aquaculture, as it allows the transport of gametes, ensures genetic improvements in desired species and minimizes asynchrony between breeding males and females. Despite the benefits, cell damage can be caused during the freezing process, causing decreased quality of sperm. Therefore, studies that seek alternatives that minimize such damage are of great importance. Antifreeze proteins (AFPs) are important compounds originated from evolutionary processes in organisms that live in polar environments. AFPs can be used as possible cryoprotective agents, as they are able to decrease ice crystals and protect the plasma membrane of cells during the freezing process. Thus, this review aims to gather information about the use of antifreeze proteins in the conservation of fish semen and present the main results found in the literature. It was observed that the use of these substances in seminal cryopreservation in fish promoted improvements in the rates of sperm motility, velocities and integrity of the plasma membrane. Such improvements were observed in an interspecific manner. Therefore, further studies are needed in order to verify the effect of AFPs on fertilization capacity, seeking the best option for supplementing the medium.(AU)


Assuntos
Animais , Masculino , Criopreservação/métodos , Criopreservação/veterinária , Sêmen , Espermatozoides , Reprodução/genética , Peixes/genética
5.
Ciênc. Anim. (Impr.) ; 30(04, Supl. 2): 337-341, 2020. tab
Artigo em Português | VETINDEX | ID: biblio-1472590

Resumo

Prochilodus brevis is a fish species with an important ecological and economic role. Therefore, its captive breeding, together with sperm cryopreservation, come as an alternative to improve its availability. To perform sperm cryopreservation with quality results, it is necessary to develop studies on cryomedia composition, such as non-permeable cryoprotectants. The aim of the study was to test the efficacy of two non-permeable cryoprotectants on post-thawed kinetic parameters of Prochilodus brevis sperm. Twenty reproductive mature P. brevis males were selected and induced to sperm with carp pituitary extract. The sperm was collected, and five pools were made. Pools were diluted in 5% glucose and 10% DMSO added or not with three different concentrations of egg yolk (5; 10 or 12%). The samples were placed on French straws, frozen in dry shipper and stored in liquid nitrogen. After thawing, the kinetic analyses of computer assisted sperm analysis (CASA) were performed. There were no significant differences between the total motility of the control (60.48%) and treatments (49.0; 47.8 and 51.68%, respectively). The other kinetic parameters also showed no statistical difference. Thus, there is no need to use egg yolk in the freezing of P. brevis sperm when using a glucose and DMSO extender.


Assuntos
Masculino , Animais , Criopreservação/métodos , Criopreservação/veterinária , Gema de Ovo/efeitos adversos , Peixes
6.
Ci. Anim. ; 30(04, Supl. 2): 337-341, 2020. tab
Artigo em Português | VETINDEX | ID: vti-32193

Resumo

Prochilodus brevis is a fish species with an important ecological and economic role. Therefore, its captive breeding, together with sperm cryopreservation, come as an alternative to improve its availability. To perform sperm cryopreservation with quality results, it is necessary to develop studies on cryomedia composition, such as non-permeable cryoprotectants. The aim of the study was to test the efficacy of two non-permeable cryoprotectants on post-thawed kinetic parameters of Prochilodus brevis sperm. Twenty reproductive mature P. brevis males were selected and induced to sperm with carp pituitary extract. The sperm was collected, and five pools were made. Pools were diluted in 5% glucose and 10% DMSO added or not with three different concentrations of egg yolk (5; 10 or 12%). The samples were placed on French straws, frozen in dry shipper and stored in liquid nitrogen. After thawing, the kinetic analyses of computer assisted sperm analysis (CASA) were performed. There were no significant differences between the total motility of the control (60.48%) and treatments (49.0; 47.8 and 51.68%, respectively). The other kinetic parameters also showed no statistical difference. Thus, there is no need to use egg yolk in the freezing of P. brevis sperm when using a glucose and DMSO extender.(AU)


Assuntos
Animais , Masculino , Criopreservação/métodos , Criopreservação/veterinária , Gema de Ovo/efeitos adversos , Peixes
7.
Ciênc. Anim. (Impr.) ; 29(1,supl.1): 70-74, 2019. tab
Artigo em Português | VETINDEX | ID: biblio-1472482

Resumo

Os pepinos do mar são equinodermatas muito importantes tanto ecologicamente quanto economicamente. A exploração desse animal, principalmente de espécimes de Holothuria grisea, tem aumentado devido à recente descoberta de moléculas bioativas de interesse para a indústria farmacêutica. O objetivo desse trabalho foi identificar microssatélites que podem ser interessantes para o manejo reprodutivo e populacional dessa espécie. Para isso foi utilizado sequenciamento de nova geração seguido de identificação pelo software SSR pipeline. Foram identificados 3 microssatélites para loci diferentes, sendo um com possível aplicação na diferenciação sexual entre espécimes, além de variabilidade genética populacional bem conservada.


Sea cucumbers are very important echinodermata both ecologically and economically. The exploitation of this animal, mainly of Holothuria grisea specimens, has increased due to the recent discovery of bioactive molecules of interest for the pharmaceutical industry. The objective of this work was to identify microsatellites that may be interesting for the reproductive and population management of this species. For this, new generation sequencing was used followed by identification by the SSR pipeline software. Three microsatellites were identified for different loci, one with possible application in the sexual differentiation between specimens, in addition to well conserved population genetic variability.


Assuntos
Animais , Genética Populacional/métodos , Manejo de Espécimes/veterinária , Pepinos-do-Mar/genética , Repetições de Microssatélites/genética
8.
Ciênc. Anim. (Impr.) ; 29(1,supl.1): 70-74, 2019. tab
Artigo em Português | VETINDEX | ID: vti-21590

Resumo

Os pepinos do mar são equinodermatas muito importantes tanto ecologicamente quanto economicamente. A exploração desse animal, principalmente de espécimes de Holothuria grisea, tem aumentado devido à recente descoberta de moléculas bioativas de interesse para a indústria farmacêutica. O objetivo desse trabalho foi identificar microssatélites que podem ser interessantes para o manejo reprodutivo e populacional dessa espécie. Para isso foi utilizado sequenciamento de nova geração seguido de identificação pelo software SSR pipeline. Foram identificados 3 microssatélites para loci diferentes, sendo um com possível aplicação na diferenciação sexual entre espécimes, além de variabilidade genética populacional bem conservada.(AU)


Sea cucumbers are very important echinodermata both ecologically and economically. The exploitation of this animal, mainly of Holothuria grisea specimens, has increased due to the recent discovery of bioactive molecules of interest for the pharmaceutical industry. The objective of this work was to identify microsatellites that may be interesting for the reproductive and population management of this species. For this, new generation sequencing was used followed by identification by the SSR pipeline software. Three microsatellites were identified for different loci, one with possible application in the sexual differentiation between specimens, in addition to well conserved population genetic variability.(AU)


Assuntos
Animais , Pepinos-do-Mar/genética , Repetições de Microssatélites/genética , Manejo de Espécimes/veterinária , Genética Populacional/métodos
9.
Acta sci. vet. (Impr.) ; 47: Pub.1665-2019. ilus, tab
Artigo em Português | VETINDEX | ID: biblio-1458063

Resumo

Background: Seminal cryopreservation is a technique that optimizes aquacultural production, as it requires less breedingand enables reproduction outside of the breeding season. This technique also helps to preserve species, thus reducing thepressure on the natural stocks. Several studies have sought to develop freezing protocols that result in semen of a goodquality. However, some studies do not evaluate the ability of frozen semen to produce viable larvae. Therefore, the aimof this study was to verify the fertilizing capacity of the frozen semen of Prochilodus brevis.Materials, Methods & Results: Semen from twenty adult males of the Brazilian bocachico was collected and evaluatedto establish the total motility, curvilinear velocity, straight linear velocity, average path velocity, membrane integrity, pH,and concentration. Six pools were formed, each of which was diluted in a freezing medium containing 5% glucose with10% dimethyl sulfoxide (DMSO) or 5% glucose with 10% methyl glycol (MG). The samples were loaded into 0.25 mLFrench straws, frozen in a dry shipper, and stored in a liquid nitrogen canister. The semen was then thawed and evaluatedto establish the total motility, curvilinear velocity, straight linear velocity, average path velocity, and membrane integrity.For the fertilization test, four females were used. The oocytes from each female were divided into three batches and fertilized with either fresh or cryopreserved semen. The rates of fertilization, hatching, and larval survival were then measured.Data were expressed as the mean ± standard deviation and analyzed using SAS (2002). The frozen semen with glucose +DMSO was significantly higher (P < 0.001) than the frozen semen with glucose + MG, in all seminal quality parametersevaluated (63.95 ± 15.88% and 25.36 ± 3.53% for the motility, 36.38 ± 7.02 μm.s-1 and 20.45 ± 2.84 μm.s-1 for the curvilinear velocity, 19.26 ± 2.74 μm.s-1 and...


Assuntos
Animais , Caraciformes , Criopreservação/veterinária , Dimetil Sulfóxido , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária
10.
Acta sci. vet. (Online) ; 47: Pub. 1665, June 16, 2019. ilus, tab
Artigo em Português | VETINDEX | ID: vti-21051

Resumo

Background: Seminal cryopreservation is a technique that optimizes aquacultural production, as it requires less breedingand enables reproduction outside of the breeding season. This technique also helps to preserve species, thus reducing thepressure on the natural stocks. Several studies have sought to develop freezing protocols that result in semen of a goodquality. However, some studies do not evaluate the ability of frozen semen to produce viable larvae. Therefore, the aimof this study was to verify the fertilizing capacity of the frozen semen of Prochilodus brevis.Materials, Methods & Results: Semen from twenty adult males of the Brazilian bocachico was collected and evaluatedto establish the total motility, curvilinear velocity, straight linear velocity, average path velocity, membrane integrity, pH,and concentration. Six pools were formed, each of which was diluted in a freezing medium containing 5% glucose with10% dimethyl sulfoxide (DMSO) or 5% glucose with 10% methyl glycol (MG). The samples were loaded into 0.25 mLFrench straws, frozen in a dry shipper, and stored in a liquid nitrogen canister. The semen was then thawed and evaluatedto establish the total motility, curvilinear velocity, straight linear velocity, average path velocity, and membrane integrity.For the fertilization test, four females were used. The oocytes from each female were divided into three batches and fertilized with either fresh or cryopreserved semen. The rates of fertilization, hatching, and larval survival were then measured.Data were expressed as the mean ± standard deviation and analyzed using SAS (2002). The frozen semen with glucose +DMSO was significantly higher (P < 0.001) than the frozen semen with glucose + MG, in all seminal quality parametersevaluated (63.95 ± 15.88% and 25.36 ± 3.53% for the motility, 36.38 ± 7.02 μm.s-1 and 20.45 ± 2.84 μm.s-1 for the curvilinear velocity, 19.26 ± 2.74 μm.s-1 and...(AU)


Assuntos
Animais , Caraciformes , Criopreservação/veterinária , Preservação do Sêmen/métodos , Preservação do Sêmen/veterinária , Dimetil Sulfóxido
11.
Ciênc. Anim. (Impr.) ; 28(3): 37-44, 2018. tab, ilus
Artigo em Português | VETINDEX | ID: vti-19417

Resumo

A presente pesquisa visou determinar a dose inseminante para a fertilização assistida da carpa comum, e o registro em imagens e o tempo de desenvolvimento de cada fase embrionária desta espécie em latitude equatorial. Verificou-se que a porcentagem de fertilização aumentou de forma linear, atingindo um platô em 45,5% na proporção de 208.295 espermatozoides/oócito. Assim, recomenda-se o uso da dose inseminante de aproximadamente 200.000 espermatozoides/oócito na rotina de fertilização assistida dessa espécie, no nordeste brasileiro. Além disso, o desenvolvimento embrionário da carpa em latitude equatorial segue a cronologia semelhante ao relatado para a espécie em clima temperado.(AU)


The present research aimed at determining insemination dose for artificial fertilization of common carp, and record images and time in the development of each embryonic stage of the species in equatorial latitude. The fertilization rate increased linearly up to the proportion of 208.295 spermatozoa/oocyte, and, from this point, the fertilization rate was maintained at 45.5%. Therefore, we recommend the use of the insemination dose of approximately 200.000 sperm/oocyte in the artificial fertilization routine of common carpin brazilian northeast. Moreover, embryonic development of carp in equatorial latitude follows the chronology similar to that reported for the species in temperate zones.(AU)


Assuntos
Animais , Carpas/embriologia , Técnicas de Reprodução Assistida/veterinária , Substâncias para o Controle da Reprodução/administração & dosagem , Reprodução , Desenvolvimento Embrionário
12.
Ciênc. Anim. (Impr.) ; 28(3): 37-44, 2018. tab, ilus
Artigo em Português | VETINDEX | ID: biblio-1472397

Resumo

A presente pesquisa visou determinar a dose inseminante para a fertilização assistida da carpa comum, e o registro em imagens e o tempo de desenvolvimento de cada fase embrionária desta espécie em latitude equatorial. Verificou-se que a porcentagem de fertilização aumentou de forma linear, atingindo um platô em 45,5% na proporção de 208.295 espermatozoides/oócito. Assim, recomenda-se o uso da dose inseminante de aproximadamente 200.000 espermatozoides/oócito na rotina de fertilização assistida dessa espécie, no nordeste brasileiro. Além disso, o desenvolvimento embrionário da carpa em latitude equatorial segue a cronologia semelhante ao relatado para a espécie em clima temperado.


The present research aimed at determining insemination dose for artificial fertilization of common carp, and record images and time in the development of each embryonic stage of the species in equatorial latitude. The fertilization rate increased linearly up to the proportion of 208.295 spermatozoa/oocyte, and, from this point, the fertilization rate was maintained at 45.5%. Therefore, we recommend the use of the insemination dose of approximately 200.000 sperm/oocyte in the artificial fertilization routine of common carpin brazilian northeast. Moreover, embryonic development of carp in equatorial latitude follows the chronology similar to that reported for the species in temperate zones.


Assuntos
Animais , Carpas/embriologia , Substâncias para o Controle da Reprodução/administração & dosagem , Técnicas de Reprodução Assistida/veterinária , Desenvolvimento Embrionário , Reprodução
13.
Acta sci. vet. (Impr.) ; 45: 1-9, 2017. tab
Artigo em Português | VETINDEX | ID: biblio-1457645

Resumo

Background: Prochilodus brevis is a rheophilic fish of economic and ecological importance. However, anthropic action has made its population vulnerable. Thus, the development of reproductive biotechnologies, such as seminal conservation, is necessary to subsidize their fish farming. However, seminal collections are often performed in places with few laboratory resources, demanding studies to determine the maximum time for which sperm can be cooled, as well as its process until frozen. Thus, the present study aimed to evaluate the influence of cooling time and the presence of dilution solutions on cryopreservation of P. brevis semen.Material, Methods & Results: After seminal collection, nine pools were formed and analyzed for seminal pH, concentration, membrane integrity, morphology and spermatic kinetics - motility, curvilinear velocity (VCL), average path velocity (VAP) and straight line velocity (VSL). After the analysis of the pools in natura (control 1), they were processed as follows: 1)- immediate freezing (control 2); 2)- cooling: undiluted, diluted in coconut water powder (ACP-104) or diluted in 5% glucose, followed by cooling at different times (6, 12, 24 or 48 h); 3)- Post-refrigeration freezing: the pools were diluted in their respective diluents and 10% dimethyl sulfoxide. After 15 days, the samples were thawed and analyzed for the aforementioned parameters. For the cooled and post-thawed semen, a completely randomized design with 2 (diluent × cooling time) and 3 (storage form × cooling time and storage form × diluent) factors, respectively, was utilized. ANOVA and Dunnett tests were applied to compare the means. In case of seminal cooling, there was no difference (P > 0.05) in sperm motility between control 1 and the undiluted and diluted treatments in ACP-104 for up to 24 h. After 48 h, only the VCL of the sample diluted in ACP-104 was similar (P > 0.05) to that of control 1.[...]


Assuntos
Masculino , Animais , Agentes de Resfriamento , Caraciformes , Diluição , Fatores de Tempo , Preservação do Sêmen/veterinária , Criopreservação/veterinária
14.
Acta sci. vet. (Online) ; 45: 1-9, 2017. tab
Artigo em Português | VETINDEX | ID: vti-20249

Resumo

Background: Prochilodus brevis is a rheophilic fish of economic and ecological importance. However, anthropic action has made its population vulnerable. Thus, the development of reproductive biotechnologies, such as seminal conservation, is necessary to subsidize their fish farming. However, seminal collections are often performed in places with few laboratory resources, demanding studies to determine the maximum time for which sperm can be cooled, as well as its process until frozen. Thus, the present study aimed to evaluate the influence of cooling time and the presence of dilution solutions on cryopreservation of P. brevis semen.Material, Methods & Results: After seminal collection, nine pools were formed and analyzed for seminal pH, concentration, membrane integrity, morphology and spermatic kinetics - motility, curvilinear velocity (VCL), average path velocity (VAP) and straight line velocity (VSL). After the analysis of the pools in natura (control 1), they were processed as follows: 1)- immediate freezing (control 2); 2)- cooling: undiluted, diluted in coconut water powder (ACP-104) or diluted in 5% glucose, followed by cooling at different times (6, 12, 24 or 48 h); 3)- Post-refrigeration freezing: the pools were diluted in their respective diluents and 10% dimethyl sulfoxide. After 15 days, the samples were thawed and analyzed for the aforementioned parameters. For the cooled and post-thawed semen, a completely randomized design with 2 (diluent × cooling time) and 3 (storage form × cooling time and storage form × diluent) factors, respectively, was utilized. ANOVA and Dunnett tests were applied to compare the means. In case of seminal cooling, there was no difference (P > 0.05) in sperm motility between control 1 and the undiluted and diluted treatments in ACP-104 for up to 24 h. After 48 h, only the VCL of the sample diluted in ACP-104 was similar (P > 0.05) to that of control 1.[...](AU)


Assuntos
Animais , Masculino , Caraciformes , Preservação do Sêmen/veterinária , Diluição , Agentes de Resfriamento , Fatores de Tempo , Criopreservação/veterinária
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA