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1.
Braz. J. Microbiol. ; 45(4): 1357-1361, Oct.-Dec. 2014. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-28456

Resumo

Several reports showed outbreaks of histoplasmosis acquired while bat-inhabited caves were visited by tourists, miners or researchers. We evaluated the performance of double immunodifusion (DI) and immunoblotting (IB) assays, employed for the histoplasmosis outbreak elucidation occurred in Vale do Paraíba, São Paulo. The existence of epidemiologic link, four patients with clinical signs suggestive of histoplasmosis and mycological confirmation has made that all 35 individuals involved to the cave visit were subjected to serological evaluation. By DI, we observed reactivity against H. capsulatum antigen in a single serum examined nearly 20 days after exposure to fungal propagules. On the other hand, IB showed reactivity against H and M fractions in 50% of samples evaluated. The analysis of the second sample batch, collected two months after the exposure showed that 96.7% were reactive by DI with antibodies titers ranging from 1 to 16 and 100% of reactivity against H and M fractions, by IB, suggesting an acute infection. The analysis of the overall agreement between the methods showed to be reasonable (κ = 0.37). This study confirms the importance and efficacy of more sensitive methodologies, such as IB assay, to early elucidation of disease, especially in cases of patients without mycological information.


Assuntos
Humanos , Masculino , Feminino , Adolescente , Adulto , Anticorpos Antifúngicos/sangue , Surtos de Doenças , Testes Diagnósticos de Rotina/métodos , Histoplasma/imunologia , Histoplasmose/diagnóstico , Histoplasmose/epidemiologia , Técnicas Microbiológicas/métodos , Brasil/epidemiologia , Imunoensaio/métodos , Sensibilidade e Especificidade
2.
J. venom. anim. toxins incl. trop. dis ; 20: 36, 04/02/2014. ilus
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-954717

Resumo

This case reports an immunocompetent 29-year-old woman with suspected pneumonia, suggestive of fungal infection. Immunoblotting analysis reactivity againstHistoplasma capsulatum and Paracoccidioides brasiliensis were observed. Nested-PCR in blood employing species-specific primers was positive for H. capsulatum andCryptococcus neoformans. The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci. In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.(AU)


Assuntos
Testes Imunológicos , Immunoblotting , Reação em Cadeia da Polimerase , Técnicas de Diagnóstico Molecular , Histoplasmose/diagnóstico , Criptococose/diagnóstico , Cryptococcus neoformans , Relatório de Pesquisa , Histoplasma
3.
Artigo em Inglês | VETINDEX | ID: vti-10986

Resumo

This case reports an immunocompetent 29-year-old woman with suspected pneumonia, suggestive of fungal infection. Immunoblotting analysis reactivity against Histoplasma capsulatum and Paracoccidioides brasiliensis were observed. Nested-PCR in blood employing species-specific primers was positive for H. capsulatum and Cryptococcus neoformans. The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci. In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.(AU)


Assuntos
Animais , Diagnóstico , Alergia e Imunologia , Histoplasma , Infecções , Reação em Cadeia da Polimerase/instrumentação
4.
Artigo em Inglês | LILACS, VETINDEX | ID: biblio-1484590

Resumo

This case reports an immunocompetent 29-year-old woman with suspected pneumonia, suggestive of fungal infection. Immunoblotting analysis reactivity against Histoplasma capsulatum and Paracoccidioides brasiliensis were observed. Nested-PCR in blood employing species-specific primers was positive for H. capsulatum and Cryptococcus neoformans. The evaluation of paucisymptomatic patients with positive results for H. capsulatum and C. neoformans could be relevant for the prevention as well as the possible evaluation of the reactivated quiescent foci. In conclusion, the associated methodology may have contributed to the monitoring endogenous reactivation of these diseases.


Assuntos
Animais , Alergia e Imunologia , Diagnóstico , Histoplasma , Infecções , Reação em Cadeia da Polimerase/instrumentação
5.
R. Inst. Adolfo Lutz ; 71(4): 747-752, 2012. tab, graf
Artigo em Português | VETINDEX | ID: vti-9192

Resumo

A histoplasmose é micose sistêmica causada por Histoplasma capsulatum. A infecção humana ocorre pela inalação de microconídios encontrados em solo contendo excretas de aves e/ou morcegos. Neste estudo foi enfatizado o potencial das atividades profissionais praticadas em cavernas em possibilitar risco de infecção por Histoplasma. Utilizando-se questionário semiestruturado, foram obtidas informações sobre o trabalho de campo executado por um grupo de 15 biólogos. A presença de anticorpos séricos anti-H. capsulatum foi avaliada em amostras de sangue coletadas desses profissionais. Na entrevista, 87% dos indivíduos relataram frequentes visitas a cavernas, 53% acampamento no interior das grutas; 33% capturade morcegos e 53% de coleta de amostras de solo. A maioria das cavernas situa-se nas regiões sudeste e centro-oeste do Brasil. A detecção de anticorpos por imunodifusão dupla demonstrou que 20% dos soros reagiram com o antígeno de H. capsulatum. Por immunoblotting, houve 94,1% de reconhecimento específico da fração de 94 kDa, sugerindo-se que os indivíduos entraram em contato com H. capsulatum. Este estudo sugere fortemente que a prática da espeleologia propicia a exposição dos indivíduos a espécies fúngicas que habitam saprofiticamente o meio ambiente, especialmente H. capsulatum, configurando-se como potencial fator de risco para infecções.(AU)


Histoplasmosis is a systemic mycosis caused by Histoplasma capsulatum. Human infection occurs by inhaling the microconidia found in soil contaminated with birds and/or bats excrements. In this study, the potential risk of infection with Histoplasma from practicing professional activities in caves was emphasized. Information on the fieldwork conducted by a group of 15 biologists were obtained through a semi-structuredquestionnaire; and the presence of antibodies anti-H. capsulatum was evaluated in their serum samples. The data analysis showed that 87% of subjects reported the frequent cave exploration, 53% camping inside the caves, 33% bats capturing activities, and 53% soil samples collection. The majority of the caves are located in the Southeast and Midwest regions of Brazil. By using double immunodiffusion assay, 20% of sera reacted with H. capsulatum antigen. By immunoblotting, it was found 94.1% of specific recognition of 94 kDa fraction, suggesting that the individuals had contact with the fungus. This study strongly suggests that the individuals practicing speleology were prone to fungal species exposure, which inhabit the environment as saprophytes, especially H. capsulatum, and it might characterize a potential risk factor for infections.(AU)


Assuntos
Humanos , Animais , Histoplasmose/patologia , Pesquisadores , Quirópteros/classificação , Aves/classificação , Doenças do Sistema Imunitário/complicações , Epidemiologia/tendências
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