Reproductive parameters and weight gain of roosters fed with waste oil from olive culture

Waste oil from olive oil extraction industry was used, instead of soybean oil, in heavy roosters' diet in order to evaluate birds' reproductive parameters. A total of forty roosters were housed individually in boxes with 1.2 m². Two experimental diets were used: control diet, based on corn, soybean meal, and soybean oil; and test diet, where soybean oil was totally replaced by waste oil. In order to verify weight gain and feed intake, animals were individually weighed weekly. Seven semen collections were performed with fifteen-day interval. Reproductive variables analyzed sperm volume, motility, concentration, and morphology. No statistical difference (p > 0.05) was observed between treatments at the different collection periods for the variables sperm volume, motility, and concentration. There was a statistically significant difference between treatments for body weight in periods three (p = 0.04), and seven (p = 0.04). Statistical differences (p = 0.01) were also observed between treatments for abnormal sperm morphology. Among collection periods, statistical difference was observed for motility (p = 0.00), and sperm concentration (p = 0.01). Total replacement of soybean oil by waste oil from olive oil extraction in young heavy roosters' diets does not affect sperm volume, motility, and concentration; reduces defects in sperm tail, and promotes better weight gain control.(AU)

Reproductive parameters and weight gain of roosters fed with waste oil from olive culture

Waste oil from olive oil extraction industry was used, instead of soybean oil, in heavy roosters diet in order to evaluate birds reproductive parameters. Atotal of forty roosters were housed individually in boxes with 1.2 m². Two experimental diets were used: control diet, based on corn, soybean meal, and soybean oil; and test diet, where soybean oil was totally replaced by waste oil. In order to verify weight gain and feed intake, animals were individually weighed weekly. Seven semen collections were performed with fifteen-day interval. Reproductive variables analyzed sperm volume, motility, concentration, and morphology. No statistical difference (p >0.05) was observed between treatments at the different collection periods for the variables sperm volume, motility, and concentration. There was a statistically significant difference between treatments for body weight in periods three (p =0.04), and seven (p=0.04). Statistical differences (p =0.01) were also observed between treatments for abnormal sperm morphology. Among collection periods, statistical difference was observed for motility (p =0.00), and sperm concentration (p =0.01). Total replacement of soybean oil by waste oil from olive oil extraction in young heavy roosters diets does not affect sperm volume, motility, and concentration; reduces defects in sperm tail, and promotes better weight gain control.(AU)

Efeito da adição de taurina sobre espermatozoides ovinos refrigerados a 5°C / Effect of taurine addiction on ram sperm cooled at 5ºC

O objetivo foi avaliar o efeito da adição de diferentes concentrações de taurina ao diluente de resfriamento de sêmen ovino. Foram utilizados neste experimento 40 ejaculados de oito carneiros, diluídos nostratamentos: controle Tris-gema (T1) adicionado de taurina nas concentrações de 1 µM (T2), 2 µM (T3) e 3 µM (T4), sob refrigeração a 5°C por 48 h e avaliados através da análise de parâmetros de qualidade espermática de integridade de membrana, DNA e acrossoma, funcionalidade de mitocôndria e motilidade espermática. Observou-se que a motilidade espermática nas 48 h foi inferior no T2 (29,3%) em relação ao T4 (37,6%) (P < 0,05). Quanto aos parâmetros de integridade de membrana e DNA não se verificou diferença estatística entre os tratamentos.Para integridade de acrossoma, em amostras de sêmen fresco, encontrou-se 59.0%, e após 48 h de refrigeração, foram observadas taxas integridade nos grupos adicionados de taurina de 50.7% (T2), 51,3% (T3) e 51.6% (T4),que não diferiram do sêmen fresco. Conclui-se que a taurina nas concentrações testadas foi eficiente para manter a integridade de acrossoma no sêmen ovino refrigerado a 5°C.(AU)

The objective of this work was to assess the effect of different concentrations of taurine added to ramsperm cooling extender. We used in this experiment 40 ejaculates from eight rams, diluted according to the following treatments: Tris-yolk control (T1) taurine-added at the concentrations of 1 µM (T2), 2 µM (T3) and 3 µM (T4), under 5º C refrigeration for 48 hours and assessed through sperm quality parameters of membrane integrity, DNA and acrosome, mitochondrial functionality and sperm motility. We observed that sperm motilitywithin 48 hours was lower in T2 (29.3%) when compared to T4 (37.6%) (P < 0.05). As for the parameters membrane integrity and DNA we did not observe statistical differences among the treatments. As for acrosome integrity, in fresh semen samples, we obtained 59.0%, and after 48 hours refrigeration we observed integrity rates in the taurine-added groups of 50.7% (T2), 51,3% (T3) and 51.6% (T4), that did not differ from fresh semen. In conclusion, the concentrations of taurine we tested was efficient to keep acrosome integrity within cooled ram sperm at 5ºC.(AU)

Efeito da adição de taurina sobre espermatozoides ovinos refrigerados a 5°C / Effect of taurine addiction on ram sperm cooled at 5ºC

O objetivo foi avaliar o efeito da adição de diferentes concentrações de taurina ao diluente de resfriamento de sêmen ovino. Foram utilizados neste experimento 40 ejaculados de oito carneiros, diluídos nostratamentos: controle Tris-gema (T1) adicionado de taurina nas concentrações de 1 µM (T2), 2 µM (T3) e 3 µM (T4), sob refrigeração a 5°C por 48 h e avaliados através da análise de parâmetros de qualidade espermática de integridade de membrana, DNA e acrossoma, funcionalidade de mitocôndria e motilidade espermática. Observou-se que a motilidade espermática nas 48 h foi inferior no T2 (29,3%) em relação ao T4 (37,6%) (P < 0,05). Quanto aos parâmetros de integridade de membrana e DNA não se verificou diferença estatística entre os tratamentos.Para integridade de acrossoma, em amostras de sêmen fresco, encontrou-se 59.0%, e após 48 h de refrigeração, foram observadas taxas integridade nos grupos adicionados de taurina de 50.7% (T2), 51,3% (T3) e 51.6% (T4),que não diferiram do sêmen fresco. Conclui-se que a taurina nas concentrações testadas foi eficiente para manter a integridade de acrossoma no sêmen ovino refrigerado a 5°C.

The objective of this work was to assess the effect of different concentrations of taurine added to ramsperm cooling extender. We used in this experiment 40 ejaculates from eight rams, diluted according to the following treatments: Tris-yolk control (T1) taurine-added at the concentrations of 1 µM (T2), 2 µM (T3) and 3 µM (T4), under 5º C refrigeration for 48 hours and assessed through sperm quality parameters of membrane integrity, DNA and acrosome, mitochondrial functionality and sperm motility. We observed that sperm motilitywithin 48 hours was lower in T2 (29.3%) when compared to T4 (37.6%) (P < 0.05). As for the parameters membrane integrity and DNA we did not observe statistical differences among the treatments. As for acrosome integrity, in fresh semen samples, we obtained 59.0%, and after 48 hours refrigeration we observed integrity rates in the taurine-added groups of 50.7% (T2), 51,3% (T3) and 51.6% (T4), that did not differ from fresh semen. In conclusion, the concentrations of taurine we tested was efficient to keep acrosome integrity within cooled ram sperm at 5ºC.