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1.
Semina ciênc. agrar ; 43(1): 51-60, jan.-fev. 2022. ilus
Artigo em Inglês | VETINDEX | ID: biblio-1368528

Resumo

Felid alphaherpesvirus type 1 (FHV-1) is an important cause of respiratory and ocular diseases in cats worldwide. Mice have been widely used to study the pathogenesis of several human and animal viruses, especially herpesviruses. This study aimed to verify whether BALB/c mice are susceptible to FHV-1 infection. The animals were intranasally inoculated with FHV-1 and their clinical signs were observed from 3 days postinfection (dpi). At 10 dpi, the animals were euthanized and the lungs, liver, spleen, and kidneys were collected for histopathological examination and quantitative polymerase chain reaction. The results showed that mice were infected with FHV-1 and reproduced several features of the disease observed in its natural host. Histological lesions and viral DNA were found in all sampled tissues, with a higher frequency of FHV-1 DNA copies detected in the lungs. All mice were seroconverted to FHV-1 at 7 dpi. To our knowledge, this is the first report of experimental infection of BALB/c mice with FHV-1. Our findings demonstrate that this murine model can contribute to understanding of FHV-1 pathogenesis and may be useful for trials against this virus.(AU)


O herpesvírus felino tipo 1 (FHV-1) é um importante agente causador de doença respiratória e ocular em gatos em todo o mundo. Camundongos têm sido amplamente utilizados para estudar a patogenia de diversos vírus humanos e animais, especialmente os herpesvírus. O objetivo deste estudo foi verificar se camundongos BALB/c poderiam ser suscetíveis a infecção pelo FHV-1. Os animais foram inoculados com FHV-1 intranasalmente e sinais clínicos foram observados a partir do dia 3 após a infecção (dpi). Após dez dias da inoculação, os animais foram eutanasiados e os pulmões, fígado, baço e rins foram coletados para exame histopatológico e PCR quantitativo (qPCR). Os resultados mostraram que os camundongos foram infectados com o FHV-1 e reproduziram várias características da doença que são observadas em seu hospedeiro natural. Lesões histológicas e DNA viral foram detectadas em todos os tecidos amostrados, com maior frequência de DNA do FHV-1 nos pulmões. Todos os camundongos soroconverteram para FHV-1 aos 7 dpi. Para nosso conhecimento, este estudo é o primeiro relato de infecção experimental de camundongos BALB/c com FHV-1. Nossos resultados demonstraram que esse camundongo pode contribuir para o entendimento da patogenia do FHV-1 e pode ser um modelo útil para ensaios contra esse vírus.(AU)


Assuntos
Animais , Reação em Cadeia da Polimerase , Di-Hidrotaquisterol , Herpesviridae , Infecções
2.
Acta sci. vet. (Impr.) ; 48(suppl.1): Pub.517-4 jan. 2020. ilus
Artigo em Inglês | VETINDEX | ID: biblio-1458344

Resumo

Background: Malformations are structural or functional abnormalities in the organs and structures present at birth. Theseconditions are rarely described in the newborns of dogs and can lead to their death. Meroanencephaly is a defect of theneural tube closure malformation, a type of anencephaly and results from a failure of closure of the rostral neuropore(neural crest), and consequently the development of the calvary becomes defective. This study aims to characterize theclinical-pathological aspects of neonatal meroanencephaly since brain malformations are rare in newborn dogs.Case: A 2-day-old English Pointer canine was sent for a necropsy. The newborn belonged to a litter of eight puppies, andonly this one had macroscopic cranial alterations. Another puppy that died as a consequence of being trampled by thebitch was also necropsied. The newborn was alive for 48 h until death and presented apathy, crying, sucking reflex andopisthotonus. Macroscopic examination of the baby revealed flattening of the skull, with a slit at the site of bone symphysis fusion, and a slit in the skin of the parietal region, covered by thin, translucent meningeal tissue. The newborn hadno other macroscopic changes. The heads of the two animals were examined by radiography to identify the features ofanencephaly in one of the animals by visualizing skull bone flattening. Upon removing the skin and exposing the cranialcavity, an irregular reddish mass was revealed, that corresponded microscopically to area cerebrovasculosa, composed ofneurons and rudimentary glial tissue, vascular neoformation and, hemorrhage and congestion. The cranial nerves was notpossible to observe. There was disorganization of the brain areas with no limitation of white and gray matter and scarceneurons and also a region similar to the cerebellum, with a molecular layer but without the Purkinje neurons. In the spinal...


Assuntos
Animais , Cães , Anencefalia/veterinária , Anormalidades Congênitas/veterinária , Crista Neural/anormalidades , Tubo Neural/anormalidades , Animais Recém-Nascidos
3.
Acta sci. vet. (Online) ; 48(suppl.1): Pub. 517, July 5, 2020. ilus
Artigo em Inglês | VETINDEX | ID: vti-33235

Resumo

Background: Malformations are structural or functional abnormalities in the organs and structures present at birth. Theseconditions are rarely described in the newborns of dogs and can lead to their death. Meroanencephaly is a defect of theneural tube closure malformation, a type of anencephaly and results from a failure of closure of the rostral neuropore(neural crest), and consequently the development of the calvary becomes defective. This study aims to characterize theclinical-pathological aspects of neonatal meroanencephaly since brain malformations are rare in newborn dogs.Case: A 2-day-old English Pointer canine was sent for a necropsy. The newborn belonged to a litter of eight puppies, andonly this one had macroscopic cranial alterations. Another puppy that died as a consequence of being trampled by thebitch was also necropsied. The newborn was alive for 48 h until death and presented apathy, crying, sucking reflex andopisthotonus. Macroscopic examination of the baby revealed flattening of the skull, with a slit at the site of bone symphysis fusion, and a slit in the skin of the parietal region, covered by thin, translucent meningeal tissue. The newborn hadno other macroscopic changes. The heads of the two animals were examined by radiography to identify the features ofanencephaly in one of the animals by visualizing skull bone flattening. Upon removing the skin and exposing the cranialcavity, an irregular reddish mass was revealed, that corresponded microscopically to area cerebrovasculosa, composed ofneurons and rudimentary glial tissue, vascular neoformation and, hemorrhage and congestion. The cranial nerves was notpossible to observe. There was disorganization of the brain areas with no limitation of white and gray matter and scarceneurons and also a region similar to the cerebellum, with a molecular layer but without the Purkinje neurons. In the spinal...(AU)


Assuntos
Animais , Cães , Anencefalia/veterinária , Tubo Neural/anormalidades , Crista Neural/anormalidades , Anormalidades Congênitas/veterinária , Animais Recém-Nascidos
4.
R. bras. Parasitol. Vet. ; 29(4): e011820, out. 2020. ilus
Artigo em Inglês | VETINDEX | ID: vti-29891

Resumo

Dioctophymosis is caused by Dioctophyme renale, a nematode that usually affects the right kidney of carnivores. The aim of this study was to report on a case of a dog with progressive weight loss and swollen abdomen that was diagnosed as presenting dioctophymosis. The patient underwent surgical treatment through which 34 nematodes were found, of which 18 were female and 16 were male, with a maximum length of 74 centimeters. The parasites were free in the abdominal cavity and inside the right kidney, and had caused peritonitis, free fluid, severe adherences between the abdominal organs and renal perforation. Parasitic diseases with a high number of specimens of this species are uncommon in dogs. The disease progresses with an inflammatory reaction and consequent formation of adherences and granulomatous tissue. This surrounds the eggs that were eliminated in the abdominal cavity by the free parasites. This disease occurs frequently in the city of Pelotas, Rio Grande do Sul, Brazil, where the patient of this report was living. To the best of authors knowledge, this is the case with the largest number of specimens of D. renale removed from a single dog in vivo.(AU)


A dioctofimose é causada por Dioctophyme renale, um nematódeo que geralmente afeta o rim direito de carnívoros. Este trabalho objetivou relatar o caso de um cão, com perda de peso progressiva e abaulamento abdominal, diagnosticado com dioctofimose. O paciente foi submetido a tratamento cirúrgico, o que permitiu o achado de 34 nematódeos, sendo 18 fêmeas e 16 machos, com tamanho máximo de 74 centímetros. Os parasitos encontravam-se livres na cavidade abdominal e no interior do rim direito, causando peritonite, formação de líquido livre, intensas aderências entre os órgãos abdominais e uma perfuração renal. Parasitoses com alto número de exemplares dessa espécie são incomuns em cães. Cursam com reação inflamatória, com consequente formação de aderências e tecido granulomatoso, envolvendo os ovos eliminados na cavidade abdominal pelos parasitos livres. A doença é frequente na cidade de Pelotas, Rio Grande do Sul, Brasil, área de origem da paciente afetada. De acordo com a pesquisa realizada, acredita-se que esse é o caso com o maior número de exemplares de D. renale removidos de um cão in vivo.(AU)


Assuntos
Animais , Cães , Cães/parasitologia , Infecções por Ascaridida/complicações , Infecções por Ascaridida/diagnóstico , Rim/parasitologia
5.
Acta sci. vet. (Online) ; 47(suppl.1): Pub. 384, 24 abr. 2019. ilus
Artigo em Português | VETINDEX | ID: vti-19572

Resumo

Background: The prevalence of intracranial neoplasms in dogs represents 2.1 to 4.0% of the cases. Brain tumors may beprimary or metastatic. The objective of this study was to describe two cases of intra encephalic neoplasia in elderly dogsreceived for necropsy by the Veterinary Oncology Service in the Federal University of Pelotas.Cases: Case 1: A 12-year-old female canine, without breed and medium size, was received for necropsy. The animal hashad behavioral changes. Macroscopic examination of the encephalus revealed asymmetry and congestion. The organs werecollected and fixed in 10% formalin. In the brain cleavage we noticed an extensive brown-gray mass with reddish areas,expansive, moderately demarcated, soft to cut and discrete hydrocephalus. Serial fragments of the brain and fragmentsof the organs were sent for processing. The slides were stained with the hematoxylin and eosin technique for histopathological analysis. At the microscopic examination, cuboidal cells were observed in the encephalus sometimes in acinar arrangements, of extensive and very limited pattern, diagnosed as ependymoma. Case 2: It is a 15-year-old, female Poodledog, with several tumors. During necropsy multiple subcutaneous nodules, mesentery, intestinal serosa, stomach and liverwere noticed. At the cut these were firm and whitish. No macroscopic changes were observed in the other organs. Fragments of organs and brain were collected and a serial section of the encephalus was performed for further processing andhistological analysis. In the histopathological analysis the masses were constituted by proliferation of sometimes roundedcells, elongated, with rounded nuclei and eosinophilic cytoplasm, allowing the diagnosis of mesothelioma. The samecell pattern was observed in other organs. In the frontal cortex of the encephalus there were small foci of cells similar tothose observed in the mesentery, as well as metastatic...(AU)


Assuntos
Animais , Idoso , Cães , Neoplasias Encefálicas/veterinária , Metástase Neoplásica , Mesotelioma/veterinária , Ependimoma/veterinária
6.
Acta sci. vet. (Impr.) ; 47(suppl.1): Pub.384-2019. ilus
Artigo em Português | VETINDEX | ID: biblio-1458148

Resumo

Background: The prevalence of intracranial neoplasms in dogs represents 2.1 to 4.0% of the cases. Brain tumors may beprimary or metastatic. The objective of this study was to describe two cases of intra encephalic neoplasia in elderly dogsreceived for necropsy by the Veterinary Oncology Service in the Federal University of Pelotas.Cases: Case 1: A 12-year-old female canine, without breed and medium size, was received for necropsy. The animal hashad behavioral changes. Macroscopic examination of the encephalus revealed asymmetry and congestion. The organs werecollected and fixed in 10% formalin. In the brain cleavage we noticed an extensive brown-gray mass with reddish areas,expansive, moderately demarcated, soft to cut and discrete hydrocephalus. Serial fragments of the brain and fragmentsof the organs were sent for processing. The slides were stained with the hematoxylin and eosin technique for histopathological analysis. At the microscopic examination, cuboidal cells were observed in the encephalus sometimes in acinar arrangements, of extensive and very limited pattern, diagnosed as ependymoma. Case 2: It is a 15-year-old, female Poodledog, with several tumors. During necropsy multiple subcutaneous nodules, mesentery, intestinal serosa, stomach and liverwere noticed. At the cut these were firm and whitish. No macroscopic changes were observed in the other organs. Fragments of organs and brain were collected and a serial section of the encephalus was performed for further processing andhistological analysis. In the histopathological analysis the masses were constituted by proliferation of sometimes roundedcells, elongated, with rounded nuclei and eosinophilic cytoplasm, allowing the diagnosis of mesothelioma. The samecell pattern was observed in other organs. In the frontal cortex of the encephalus there were small foci of cells similar tothose observed in the mesentery, as well as metastatic...


Assuntos
Animais , Idoso , Cães , Ependimoma/veterinária , Mesotelioma/veterinária , Metástase Neoplásica , Neoplasias Encefálicas/veterinária
7.
Semina Ci. agr. ; 38(5): 3383-3386, Set.-Out. 2017. ilus
Artigo em Inglês | VETINDEX | ID: vti-24934

Resumo

This paper to describe a case of dysontogenic urethral metaplasia in a one month old mongrel feline who also had bilateral renal dysplasia. Dysontogenic metaplasia in cats are scarce and this change may be associated with renal dysplasia and/or lower urinary tract. The animal had history of abdominal enlargement since birth and dysuria, eliminating urine only dropwise. Due to the poor prognosis we opted for euthanasia. At necropsy was observed enlarged and distended bladder, reduced kidneys and dilated and tortuous ureters. The urethra was thickened, hard to cut, and histologically, was replacing the connective tissue, cartilage and endochondral ossification areas, which features dysontogenic metaplasia. Both kidneys presented primitive appearance featuring dysplasia. Dysontogenic metaplasia in urinary tract feline with renal dysplasia, has not been described.(AU)


Este trabalho tem como objetivo descrever um caso de metaplasia disontogênica uretral em um felino sem raça definida de um mês de idade, que também apresentava displasia renal bilateral. Dados sobre metaplasia disontogênica em felinos são escassos e a alteração pode estar associada a displasia renal e/ou do trato urinário inferior. O animal apresentava histórico de aumento abdominal desde o nascimento e disúria, eliminando a urina apenas por gotejamento. Devido ao prognóstico desfavorável optou-se pela eutanásia. Na necropsia observou-se bexiga aumentada e dilatada, rins diminuídos e ureteres dilatados e tortuosos. A uretra apresentava-se espessada e firme ao corte, e histologicamente, havia substituição ao tecido conjuntivo, áreas de cartilagem e zonas de ossificação endocondral, o que caracteriza metaplasia disontogênica. Ambos os rins apresentavam aspecto primitivo caracterizando a displasia renal. A metaplasia disontogênica no trato urinário de felinos com displasia renal, não tem sido descrita.(AU)


Assuntos
Animais , Gatos , Gatos/anormalidades , Gatos/fisiologia , Metaplasia/veterinária , Nefropatias/diagnóstico , Nefropatias/veterinária
8.
R. bras. Parasitol. Vet. ; 26(1): 119-121, jan.-mar. 2017.
Artigo em Inglês | VETINDEX | ID: vti-732625

Resumo

Dioctophyme renale is a zoonotic nematode that parasites the kidneys of wild and domestic carnivores, and it has been reported frequently in Brazil. The aim here was to register the number of cases of dogs and cats diagnosed with dioctophymosis by necropsy (1981 to 2014) and ultrasound examination (2010 to 2015) in Pelotas-RS. In this context, a survey was conducted on dioctophymosis cases diagnosed at the Veterinary Pathology Laboratory (LPV) and Veterinary Clinical Hospital (HCV) of the Federal University of Pelotas (UFPel), and at a specialist veterinary imaging diagnostics clinic. In total, 95 cases were registered. The high series of the disease in dogs can be related to the presence of a large number of stray and semi-domestic dogs in the city, and also due to the ingestion of intermediate hosts of D. renale parasitized with the infective larvae. Thus, it can be concluded that Pelotas is a city with favorable conditions for the occurrence of dioctophymosis with high rate of disease in recent years.(AU)


Dioctophyme renale é um nematódeo zoonótico que parasita principalmente o rim de carnívoros silvestres e domésticos, e tem sido relatado com frequência no Brasil. Objetivou-se registrar o número de casos de cães e gatos diagnosticados com dioctofimatose por necropsia (1981 a 2014) e exame ultrassonográfico (2010 a 2015) em Pelotas-RS. Nesse contexto, foi realizado um inquérito de casos de dioctofimatose em animais de companhia no Laboratório de Patologia Veterinária (LPV) e no Hospital de Clínicas Veterinária (HCV) da Universidade Federal de Pelotas (UFPel), e em uma clínica veterinária especializada em diagnóstico por imagem, totalizando 95 casos registrados. A elevada casuística da doença em cães pode estar relacionada à presença de um grande número de cães errantes e semi-domiciliados na cidade, e também devido à ingestão dos hospedeiros intermediários de D. renale parasitados com a larva infectante. Assim, conclui-se que Pelotas é uma cidade propícia para a ocorrência de dioctofimatose, com elevada casuística da doença nos últimos anos.(AU)


Assuntos
Animais , Gatos , Cães , Nematoides/parasitologia , Autopsia/veterinária , Infecções por Enoplida/epidemiologia , Dioctophymatoidea , Parasitos/crescimento & desenvolvimento , Parasitos/parasitologia , Brasil , Ultrassonografia/veterinária
9.
Acta sci. vet. (Online) ; 45: 01-07, 2017. ilus
Artigo em Português | VETINDEX | ID: vti-691119

Resumo

Background: Dioctophymiasis is a disease caused by the nematode Dioctophyme renale and is a relatively common condition in dogs. The parasite affects the kidney, especially the right, enters the kidney capsule and causes destruction and atrophy of the parenchyma. The lesion severity depends on the amount of parasites affecting the kidney, the duration of the infection, number of kidneys involved and concurrent occurrence of kidney disease. The diseases clinical presentation may be asymptomatic or with nonspecific clinical signs. The diagnosis is based on ultrasound examination and the detection of eggs in urine, however, diagnosis is often reached only through necropsy or histopathology. This study aimed to analyze the dog kidney anatomical and pathological changes when parasitized by Dioctophyme renale. Materials, Methods & Results: The kidneys of 21 dogs diagnosed with dioctophymiasis were nephrectomized, analyzed by ultrasound and forwarded to macro and microscopic analysis. Macroscopically, the kidney size was measured as well as its renal capsule thicknes. The presences of dilatation of the renal pelvis and ureter, as well as changes of the capsule, were also observed. These fragments were collected and submitted for routine analysis and stained with hematoxylin and eosin. Histopathological examination was performed blindly by three evaluators. The intensity of [...](AU)


Assuntos
Animais , Cães , Rim/parasitologia , Infecções por Enoplida/veterinária , Dioctophymatoidea/parasitologia , Nefropatias/veterinária , Metaplasia
10.
Acta sci. vet. (Impr.) ; 45: 01-07, 2017. ilus
Artigo em Português | VETINDEX | ID: biblio-1457575

Resumo

Background: Dioctophymiasis is a disease caused by the nematode Dioctophyme renale and is a relatively common condition in dogs. The parasite affects the kidney, especially the right, enters the kidney capsule and causes destruction and atrophy of the parenchyma. The lesion severity depends on the amount of parasites affecting the kidney, the duration of the infection, number of kidneys involved and concurrent occurrence of kidney disease. The diseases clinical presentation may be asymptomatic or with nonspecific clinical signs. The diagnosis is based on ultrasound examination and the detection of eggs in urine, however, diagnosis is often reached only through necropsy or histopathology. This study aimed to analyze the dog kidney anatomical and pathological changes when parasitized by Dioctophyme renale. Materials, Methods & Results: The kidneys of 21 dogs diagnosed with dioctophymiasis were nephrectomized, analyzed by ultrasound and forwarded to macro and microscopic analysis. Macroscopically, the kidney size was measured as well as its renal capsule thicknes. The presences of dilatation of the renal pelvis and ureter, as well as changes of the capsule, were also observed. These fragments were collected and submitted for routine analysis and stained with hematoxylin and eosin. Histopathological examination was performed blindly by three evaluators. The intensity of [...]


Assuntos
Animais , Cães , Dioctophymatoidea/parasitologia , Infecções por Enoplida/veterinária , Rim/parasitologia , Metaplasia , Nefropatias/veterinária
11.
Ci. Rural ; 46(8): 1443-1449, ago. 2016. ilus, tab
Artigo em Inglês | VETINDEX | ID: vti-22517

Resumo

In dogs, diseases of the urinary tract are common and can be caused by disorders of varied etiology. The objective of this study was to classify qualitatively and quantitatively urinary tract lesions of 363 dogs, which were classified according to its anatomical distribution and etiology. The data was obtained from the revision of 36 years of protocols from the Regional Laboratory of Diagnosis (LRD/UFPel) and it represents 4.0% of diagnoses from a total of 8980 for that period and species. Renal injury accounted for 93.1% of cases, with 309 being primary kidney lesions; from which the main lesions were the tubulointerstitial nephritis (142 cases) often associated with Leptospirosis (47). Injuries of lower urinary tract accounted for 6.9% of the cases where acute cystitis stands out (19). In this study, renal failure, acute or chronic, represented an important cause of death in dogs.(AU)


Em cães, as doenças do trato urinário são frequentes e podem ser causadas por desordens de etiologia variada. O objetivo deste trabalho foi classificar qualitativa e quantitativamente lesões do trato urinário de 363 cães, as quais foram classificadas de acordo com a distribuição anatômica e etiologia. Os dados foram obtidos em uma revisão de protocolos de 36 anos do LRD/UFPel e corresponderam a 4,0% do total de 8980 diagnósticos realizados no período para a espécie. As lesões renais representaram 93,1%, sendo 309 primárias do rim; dentre as principais lesões, está a nefrite tubulo-intersticial (142 casos), geralmente associada à Leptospirose (47). O trato urinário inferior representou 6,9% dos casos, destacando-se cistite aguda (19). Neste estudo, a insuficiência renal, aguda ou crônica, representou importante causa mortis em cães.(AU)


Assuntos
Animais , Cães , Sistema Urinário/lesões , Sistema Urinário/patologia , Rim/lesões , Nefrite/epidemiologia , Nefrite/etiologia , Nefrite/patologia , Nefrite/veterinária , Métodos Epidemiológicos/veterinária , Cistite/veterinária , Leptospirose/veterinária
12.
Artigo em Português | VETINDEX | ID: biblio-1489719

Resumo

A produção de fitoterápicos exige que em seu desenvolvimento haja testes em relação a sua toxicidade. Assim métodos que promovam a redução de animais em pesquisa devem ser aplicados também a estes compostos. A OECD (Guideline for the Testing of Chemicals) recomenda o teste do olho de frango como uma alternativa de triagem, antes de levar os produtos à ensaios in vivo. Os objetivos deste estudo foram determinar a toxicidade do extrato aquoso de Walteria ouradinha através de ensaio ex vivo em globo ocular de frangos, e demonstrar dados que justifiquem a utilização deste como teste de triagem anterior ao ensaio in vivo. Foram utilizados olhos de frangos ex vivo, distribuídos em grupos conforme o produto instilado em sua superfície: grupo extrato aquoso de W. douradinha (WD), salina 0,9% (CN) e ácido acético 10% (CP). A toxicidade foi avaliada para retenção de fluoresceína, opacidade e inchaço de córnea, e critérios histopatológicos, conforme recomendação da OECD. O grupo WD demonstrou resultados macroscópicos com baixo índice de irritabilidade, quando comparados com os olhos CP (P<0,05). Ainda, os olhos WD demonstraram menores alterações histopatológicas que os olhos CP, estando, em todas ocasiões, com índice não irritante. Nas condições deste estudo concluímos que o extrato aquoso de W. douradinha possui baixo grau de toxicidade perante as análises macroscópicas e histopatológicas. Mais que isso, indicamos o teste do olho da galinha como ensaio de triagem para determinação da toxicidade de fitoterápicos, podendo ser usado antes de levar os extratos à teste em animais, reduzindo assim o uso destes.


Requirements for the toxicological assessment of phytotherapies are increasing, as these become more widespread. Methods that reduce the animals used in these toxicology studies should also be applied for these compounds. The enucleated chicken eye test is recommended by OECD as an early screening method, used prior to the in vivo assays. The objectives of this study were to assess the toxicity of the aqueous extract of Walteria douradinha through the enucleated chicken eye test, as recommended by the OECD, and demonstrate evidence supporting it as a valid screening assay. The eyes of commercially slaughtered chicken were used in this study, these were divided into groups according to the product instilled: aqueous extract of W. douradinha (WD); 0.9% saline (CN); and 10% acetic acid (CP). Toxicity was evaluated by predetermined criteria for fluorescein retention, corneal opacity, and swelling, in five time points. Histopathological criteria for corneal lesions were also assessed. Throughout the study the WD group showed low rates of irritability compared with CP eyes (P <0.05) in the macroscopic criteria. Moreover, WD eyes showed lower histopathological changes than CP eyes, and were characterized as non irritant. In this study we conclude that the aqueous extract of W. douradinha has a low degree of toxicity, as shown by both macroscopic and histopathological parameters. Furthermore, we recommend the enucleated chicken eye test as a screening assay to determine the toxicity of herbal extracts, and should be used before in vivo testing, thus reducing the number of animals used.


Assuntos
Malvaceae/intoxicação , Malvaceae/toxicidade , Medicamento Fitoterápico , Experimentação Animal , Extratos Vegetais/análise
13.
Ci. Rural ; 44(7): 1249-1252, July 2014. ilus
Artigo em Inglês | VETINDEX | ID: vti-26972

Resumo

This study describes the natural poisoning of goats by Rhododendron simsii in Southern Brazil. Five of eight goats died after ingesting branches of plants, which were pruned and placed near the goats. Clinical signs observed were restlessness, shrill bleating, profuse regurgitation, looking and kicking at the flank, a loss of balance, irregular gait, staggering, and opisthotonos. At necropsy or histopathology affected goats had not showed lesions. Two goats were fed with 21.5g kg-1 bw and 30g kg-1 bw of fresh leaves of R. simsii. Both goats developed signs similar to those naturally affected goats. These findings indicated that R. simsii is highly toxic and lethal to goats.(AU)


Descreve-se um surto de intoxicação espontânea por R. simsii em caprinos no sul do Brasil. Cinco de oito cabras morreram após a ingestão da planta que foi podada e colada no local onde estavam os caprinos. Os sinais clínicos caracterizam-se por excitação, balidos estridentes, regurgitação, coicear o flanco, desequilíbrio e opistótono. Não foram observadas lesões macroscópicas e histológicas nos animais necropsiados. Dois animais receberam doses de 21,5 e 30g kg-1 de folhas verdes de R. simsii. Ambos desenvolveram sinais clínicos similares aos animais intoxicados espontaneamente. Esses achados indicam que R. simsii é altamente tóxica e letal para caprinos.(AU)


Assuntos
Animais , Ruminantes , Rhododendron/toxicidade , Plantas Tóxicas , Intoxicação por Plantas/veterinária , Comportamento Alimentar , Brasil
14.
R. Soc. bras. Ci. Anim. Lab. ; 2(4): 264-272, 2014.
Artigo em Português | VETINDEX | ID: vti-11538

Resumo

A produção de fitoterápicos exige que em seu desenvolvimento haja testes em relação a sua toxicidade. Assim métodos que promovam a redução de animais em pesquisa devem ser aplicados também a estes compostos. A OECD (Guideline for the Testing of Chemicals) recomenda o teste do olho de frango como uma alternativa de triagem, antes de levar os produtos à ensaios in vivo. Os objetivos deste estudo foram determinar a toxicidade do extrato aquoso de Walteria ouradinha através de ensaio ex vivo em globo ocular de frangos, e demonstrar dados que justifiquem a utilização deste como teste de triagem anterior ao ensaio in vivo. Foram utilizados olhos de frangos ex vivo, distribuídos em grupos conforme o produto instilado em sua superfície: grupo extrato aquoso de W. douradinha (WD), salina 0,9% (CN) e ácido acético 10% (CP). A toxicidade foi avaliada para retenção de fluoresceína, opacidade e inchaço de córnea, e critérios histopatológicos, conforme recomendação da OECD. O grupo WD demonstrou resultados macroscópicos com baixo índice de irritabilidade, quando comparados com os olhos CP (P<0,05). Ainda, os olhos WD demonstraram menores alterações histopatológicas que os olhos CP, estando, em todas ocasiões, com índice não irritante. Nas condições deste estudo concluímos que o extrato aquoso de W. douradinha possui baixo grau de toxicidade perante as análises macroscópicas e histopatológicas. Mais que isso, indicamos o teste do olho da galinha como ensaio de triagem para determinação da toxicidade de fitoterápicos, podendo ser usado antes de levar os extratos à teste em animais, reduzindo assim o uso destes. (AU)


Requirements for the toxicological assessment of phytotherapies are increasing, as these become more widespread. Methods that reduce the animals used in these toxicology studies should also be applied for these compounds. The enucleated chicken eye test is recommended by OECD as an early screening method, used prior to the in vivo assays. The objectives of this study were to assess the toxicity of the aqueous extract of Walteria douradinha through the enucleated chicken eye test, as recommended by the OECD, and demonstrate evidence supporting it as a valid screening assay. The eyes of commercially slaughtered chicken were used in this study, these were divided into groups according to the product instilled: aqueous extract of W. douradinha (WD); 0.9% saline (CN); and 10% acetic acid (CP). Toxicity was evaluated by predetermined criteria for fluorescein retention, corneal opacity, and swelling, in five time points. Histopathological criteria for corneal lesions were also assessed. Throughout the study the WD group showed low rates of irritability compared with CP eyes (P <0.05) in the macroscopic criteria. Moreover, WD eyes showed lower histopathological changes than CP eyes, and were characterized as non irritant. In this study we conclude that the aqueous extract of W. douradinha has a low degree of toxicity, as shown by both macroscopic and histopathological parameters. Furthermore, we recommend the enucleated chicken eye test as a screening assay to determine the toxicity of herbal extracts, and should be used before in vivo testing, thus reducing the number of animals used. (AU)


Assuntos
Malvaceae/intoxicação , Malvaceae/toxicidade , Medicamento Fitoterápico , Extratos Vegetais/análise , Experimentação Animal
15.
Pesqui. vet. bras ; 33(8): 1004-1008, ago. 2013. ilus, tab
Artigo em Português | VETINDEX | ID: vti-8608

Resumo

Descreve-se um surto de intoxicação por Amaranthus hybridus ocorrido em bovinos em fevereiro/março de 2013 na região sul do Rio Grande do Sul. A morbidade foi de 48,33%, a mortalidade de 41,66% e a letalidade 86,20%. O diagnóstico da intoxicação por A. hybridus foi baseado nos dados epidemiológicos, sinais clínicos, achados de necropsia e lesões histológicas características da intoxicação por plantas nefrotóxicas e pela presença da planta em grande quantidade na área onde estavam os bovinos. A invasão da pastagem de Brachiaria sp. pela planta alerta para a possibilidade da ocorrência da intoxicação não somente em áreas de resteva com carência de forragem, uma vez que no surto relatado neste trabalho havia abundante disponibilidade de forragem nas áreas onde os bovinos se intoxicaram. No presente caso provavelmente as boas condições climáticas e o solo fertilizado para a plantação da pastagem favoreceram o crescimento da invasora.(AU)


An outbreak of Amaranthus hybridus poisoning in cattle was observed in February/March 2013 in southern Rio Grande do Sul, Brazil. The morbidity rate observed was 48.33%, the mortality rate was 41.66% and lethality rate was 86.20%. The diagnosis of A. hybridus poisoning was made based on the epidemiological data, clinical signs, macroscopic and histological lesions characteristic of intoxication by nephrotoxic plants and by the presence of large amounts of the plant in the area where cattle were placed. Invasion of Brachiaria sp. pasture by A. hybridus alerts for the possibility of the occurrence of poisoning not only in stubble areas with lack of forage. In the case reported here there was abundant forage availability in the area where the outbreak occurred. Favorable climatic conditions and fertile soil for planting pasture, probably favored the growth of this invasive species.(AU)


Assuntos
Animais , Bovinos , Amaranthus/toxicidade , Plantas Tóxicas/toxicidade , Bovinos/classificação
16.
Nosso clínico ; 19(114): 44-48, nov./dez. 2016. ilus
Artigo em Português | VETINDEX | ID: biblio-1485962

Resumo

La intoxicación de los felinos puede ser accidental o intencional. Muchas sustâncias pueden causar síntomas similares, sin embargo, la literatura sobre el tema es escassa y el tratamiento para cada cuadro clínico es muy diferente. En este contexto, este estudio tuvo como objetivo determinar las lesiones patológicas de las intoxicaciones en los gatos en el área cubierta por el Laboratorio Regional de Diagnóstico de la Facultad de Veterinaria de la Universidad Federal de Pelotas, mediante la revisión de los archivos de laboratorio y necropsias realizadas. Se estudiaron 24 casos de intoxicación. La historia clínica fue fundamental para establecer el diagnóstico, clínica y signos y lesiones características. Entre las sustancias involucradas estabanlos derivados de lacumarina, la estricnina, etilenglicol, fluoroacetato sódio, goma de moqueta, antipulgas y Lilium sp.


Poisoning in cats can be accidental or intentional. Many substances can cause similar symptoms. And the literature on the subject are scarce. However, the treatment for each clinical features is very different and yet, the literature on the subject are scarce. This study aimed to determine the pathologicallesions of poisoning in cats in the area covered by the Regional Diagnostic Laboratory of the Veterinary School of the Federal University of Pelotas, by reviewing laboratory files and necropsies. 24 cases of poisoning were studied. The clinical history was fundamental to establish the diagnose, clinical signs and characteristic lesions. The substances involved were coumarin derivatives, strychnine, ethylene glycol, sodium fluoroacetate, carpet glue, anti-fleas and Lilium sp.


As intoxicações em felinos podem ser acidentais ou intencionais. Muitas substâncias podem provocar sintomas semelhantes, e os dados da literatura sobre o assunto são escassos. No entanto o tratamento indicado para cada quadro clínico é diferente. Neste contexto este trabalho teve como objetivo determinar as lesões anatomopatológicas das intoxicações em gatos na área de abrangência do Laboratório Regional de Diagnóstico da Faculdade de Veterinária da Universidade Federal Pelotas, através da revisão de arquivos do laboratório e necropsias realizadas. Foram estudados 24 casos de intoxicação. A história clínica foi fundamental para o estabelecimento do diagnóstico, além de sinais clínicos e lesões características. As substâncias implicadas foram derivadoscumarínicos, estricnina, etilenoglicol, fluoracetato, cola de carpete, antipulgas e Lilium sp.


Assuntos
Animais , Felidae , Intoxicação/diagnóstico , Intoxicação/veterinária , Estricnina/intoxicação , Lilium/intoxicação
17.
Pesqui. vet. bras ; 29(3): 211-214, 2009.
Artigo em Português | VETINDEX | ID: vti-453

Resumo

Descreve-se um surto de intoxicação por organofosforados (ORFs) em búfalos (Bubalus bubalis) na região sul do Rio Grande do Sul. Os animais foram submetidos à aplicação de Expertan® pour-on (clorpirifós) na dose de 12mg/kg de peso animal para controle de infestação por piolhos e desenvolveram sinais clínicos de intoxicação em um período variável entre 7-45 dias após a exposição ao produto. Os sinais clínicos caracterizaram-se por anorexia, diarréia, salivação intensa, incoordenação motora, tremores musculares, paresia e paralisia flácida, decúbito lateral e morte. Em conseqüência da intoxicação morreram 61 búfalos de um total de 267 animais sob risco. Foram realizadas três necropsias de búfalos que morreram 24-72 horas após o início dos sinais clínicos. As lesões macroscópicas caracterizaram-se por congestão e hemorragias intestinais, enfisema e edema pulmonares. Não foram observadas alterações microscópicas significativas em nenhum dos animais. Apesar da ausência de alterações histológicas no sistema nervoso central e periférico, os achados epidemiológicos, clínicos, macroscópicos e toxicológicos sugerem o diagnóstico de neurotoxicidade tardia induzida por ORFs.(AU)


This paper describes organophosphate (ORF) poisoning in a herd of water buffaloes (Bubalus bubalis) in southern Brazil, which were treated with a single dose of 12mg/kg body weight of Expertan® pour-on (chlorpyriphos) to control ectoparasites. Clinical signs, observed 7-45 days after exposure, were diarrhea, hypersalivation, ataxia, muscular tremors, weakness of pelvic limbs, paresis and flaccid paralysis and lateral recumbence. Out of 267 buffaloes 61 died. Necropsy of three animals that died 24-72 hours after onset of clinical signs, revealed congestion and serosal hemorrhages scattered along the bowel, emphysema and edema of the lungs. No significant histopathological changes were found. Residues of chlorpyriphos were detected in liver, kidneys and nervous system of the one necropsied buffalo. Despite the absence of histological lesions in the central and peripheral nervous system, the epidemiological, clinical, gross and toxicological findings suggest delayed neurotoxicity induced by organophosphates.(AU)


Assuntos
Animais , Compostos Organofosforados/efeitos adversos , Compostos Organofosforados/toxicidade , Síndromes Neurotóxicas/epidemiologia , Búfalos
18.
Semina ciênc. agrar ; 34(6): 3929-3934, 2013.
Artigo em Português | LILACS-Express | VETINDEX | ID: biblio-1433419

Resumo

The first case in Brazil of vaginal TVT with metastasis for the central nervous system is reported, as well as the presence of cutaneous, eye and lung metastases and describes the resistance of the TVT to treatment with vincristine. The TVT is a contagious neoplasm that occurs mainly in places where there is no dog population control, and currently, resistance of these tumor cells to conventional treatment with vincristine has been demonstrated. A mongrel female dog of about five years old, weighing 9.2 kg, with a history of vaginal bleeding for more than six months was attended. After cytological diagnosis, treatment with chemotherapy was recommended. There was resistance to chemotherapy treatment with vincristine sulfate, advocating the use of doxorubicin. It is suspected that tumor development in the central nervous system possibly occurred because the doxorubicin was not capable of crossing the hematoencephalic barrier, along with resistance to vincristine.


Relata-se o primeiro caso no Brasil de tumor venéreo transmissível (TVT) vaginal com metástase para o sistema nervoso central (SNC), além do envolvimento do tecido cutâneo, ocular e pulmonar e descreve a resistência do TVT ao tratamento com vincristina. O TVT é uma neoplasia contagiosa ocorrendo, principalmente, em locais em que não ocorre um controle populacional de cães, e atualmente tem sido demonstrada resistência destas células tumorais ao tratamento convencional com vincristina. Foi atendida uma fêmea canina, sem raça definida, com cerca de cinco anos de idade, pesando 9,2kg, com histórico de sangramento vaginal há mais de seis meses. Após diagnóstico citológico de TVT foi recomendado tratamento com quimioterápico. Houve resistência a quimioterapia com sulfato de vincristina, preconizando a utilização de quimioterápico doxorrubicina. Suspeita-se que o desenvolvimento do tumor no sistema nervoso central possivelmente tenha ocorrido devido à doxorrubicina não ter capacidade de atravessar a barreira hematoencefálica, aliado à resistência à vincristina.

19.
Semina ciênc. agrar ; 38(5): 3383-3386, 2017.
Artigo em Inglês | LILACS-Express | VETINDEX | ID: biblio-1433478

Resumo

This paper to describe a case of dysontogenic urethral metaplasia in a one month old mongrel feline who also had bilateral renal dysplasia. Dysontogenic metaplasia in cats are scarce and this change may be associated with renal dysplasia and/or lower urinary tract. The animal had history of abdominal enlargement since birth and dysuria, eliminating urine only dropwise. Due to the poor prognosis we opted for euthanasia. At necropsy was observed enlarged and distended bladder, reduced kidneys and dilated and tortuous ureters. The urethra was thickened, hard to cut, and histologically, was replacing the connective tissue, cartilage and endochondral ossification areas, which features dysontogenic metaplasia. Both kidneys presented primitive appearance featuring dysplasia. Dysontogenic metaplasia in urinary tract feline with renal dysplasia, has not been described.


Este trabalho tem como objetivo descrever um caso de metaplasia disontogênica uretral em um felino sem raça definida de um mês de idade, que também apresentava displasia renal bilateral. Dados sobre metaplasia disontogênica em felinos são escassos e a alteração pode estar associada a displasia renal e/ou do trato urinário inferior. O animal apresentava histórico de aumento abdominal desde o nascimento e disúria, eliminando a urina apenas por gotejamento. Devido ao prognóstico desfavorável optou-se pela eutanásia. Na necropsia observou-se bexiga aumentada e dilatada, rins diminuídos e ureteres dilatados e tortuosos. A uretra apresentava-se espessada e firme ao corte, e histologicamente, havia substituição ao tecido conjuntivo, áreas de cartilagem e zonas de ossificação endocondral, o que caracteriza metaplasia disontogênica. Ambos os rins apresentavam aspecto primitivo caracterizando a displasia renal. A metaplasia disontogênica no trato urinário de felinos com displasia renal, não tem sido descrita.

20.
Semina Ci. agr. ; 34(6): 3929-3934, 2013.
Artigo em Português | VETINDEX | ID: vti-763047

Resumo

 The first case in Brazil of vaginal TVT with metastasis for the central nervous system is reported, as well as the presence of cutaneous, eye and lung metastases and describes the resistance of the TVT to treatment with vincristine. The TVT is a contagious neoplasm that occurs mainly in places where there is no dog population control, and currently, resistance of these tumor cells to conventional treatment with vincristine has been demonstrated. A mongrel female dog of about five years old, weighing 9.2 kg, with a history of vaginal bleeding for more than six months was attended. After cytological diagnosis, treatment with chemotherapy was recommended. There was resistance to chemotherapy treatment with vincristine sulfate, advocating the use of doxorubicin. It is suspected that tumor development in the central nervous system possibly occurred because the doxorubicin was not capable of crossing the hematoencephalic barrier, along with resistance to vincristine. 


Relata-se o primeiro caso no Brasil de tumor venéreo transmissível (TVT) vaginal com metástase para o sistema nervoso central (SNC), além do envolvimento do tecido cutâneo, ocular e pulmonar e descreve a resistência do TVT ao tratamento com vincristina. O TVT é uma neoplasia contagiosa ocorrendo, principalmente, em locais em que não ocorre um controle populacional de cães, e atualmente tem sido demonstrada resistência destas células tumorais ao tratamento convencional com vincristina. Foi atendida uma fêmea canina, sem raça definida, com cerca de cinco anos de idade, pesando 9,2kg, com histórico de sangramento vaginal há mais de seis meses. Após diagnóstico citológico de TVT foi recomendado tratamento com quimioterápico. Houve resistência a quimioterapia com sulfato de vincristina, preconizando a utilização de quimioterápico doxorrubicina. Suspeita-se que o desenvolvimento do tumor no sistema nervoso central possivelmente tenha ocorrido devido à doxorrubicina não ter capacidade de atravessar a barreira hematoencefálica, aliado à resistência à vincristina. 

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