Cat epididymal semen cryopreserved with and without vitamin E: effect on sperm parameters and lipid peroxidation

The aims of this study were to investigate: 1) if the addition of α-tocopherol (vitamin E) in three concentrations (0.3, 0.6 and 0.9 mM) is able to preserve spermatozoa integrity after thawing and 2) the effect of α-tocopherol supplementation on lipid peroxidation. Fifty four domestic cats were used in this study constituting 18 pools (3 cats per pool). Each pool was submitted at four experimental groups: group 0 (control) – epididymal sperm were frozen with a commercial Botucrio® extender; group 0.3, group 0.6 and group 0.9 – the extender was supplemented with 0.3, 0.6 and 0.9 mM of α-tocopherol, respectively. Each semen sample was evaluated for motility, progressive forward motility, morphology, sperm viability (plasma membrane integrity-PMI), hypo-osmotic swelling test (HOST), before and after thawing. The evaluation of lipid peroxidation reaction by Thiobarbituric Acid Reactive Substances (TBARS) test was performed on thawed semen only. Results demonstrated that there was no significant difference between control and the three α-tocopherol groups with regards to motility and progressive motility after thawing (P > 0.05). As expected, in fresh samples viability was significantly higher than in all the cryopreserved groups in which there was no positive influence of any of the αtocopherol concentration used. Lipid peroxidation was higher in the supplemented groups 0.6 and 0.9 mM of α-tocopherol than in control and in 0.3 mM group. In conclusion, the addition of α-tocopherol to the commercial extender had no positive influence on reduction of lipid peroxidation. This topic deserves further investigations to better understand the effect of cryopreservation procedures on epididymal spermatozoa and to establish adequate strategies to counteract sperm cryodamages.

Cat epididymal semen cryopreserved with and without vitamin E: effect on sperm parameters and lipid peroxidation

The aims of this study were to investigate: 1) if the addition of α-tocopherol (vitamin E) in three concentrations (0.3, 0.6 and 0.9 mM) is able to preserve spermatozoa integrity after thawing and 2) the effect of α-tocopherol supplementation on lipid peroxidation. Fifty four domestic cats were used in this study constituting 18 pools (3 cats per pool). Each pool was submitted at four experimental groups: group 0 (control) epididymal sperm were frozen with a commercial Botucrio® extender; group 0.3, group 0.6 and group 0.9 the extender was supplemented with 0.3, 0.6 and 0.9 mM of α-tocopherol, respectively. Each semen sample was evaluated for motility, progressive forward motility, morphology, sperm viability (plasma membrane integrity-PMI), hypo-osmotic swelling test (HOST), before and after thawing. The evaluation of lipid peroxidation reaction by Thiobarbituric Acid Reactive Substances (TBARS) test was performed on thawed semen only. Results demonstrated that there was no significant difference between control and the three α-tocopherol groups with regards to motility and progressive motility after thawing (P > 0.05). As expected, in fresh samples viability was significantly higher than in all the cryopreserved groups in which there was no positive influence of any of the αtocopherol concentration used. Lipid peroxidation was higher in the supplemented groups 0.6 and 0.9 mM of α-tocopherol than in control and in 0.3 mM group. In conclusion, the addition of α-tocopherol to the commercial extender had no positive influence on reduction of lipid peroxidation. This topic deserves further investigations to better understand the effect of cryopreservation procedures on epididymal spermatozoa and to establish adequate strategies to counteract sperm cryodamages.(AU)

Resection of vaginal neoplasms by video-vaginoscopy in bitches

Background: Vaginal neoplasms usually represent a challenge for veterinary surgeons. Surgical resection often requires episiotomy or even laparotomy and pubic osteotomy, increasing the risk for intra and postoperative complications, such as severe pain, bleeding, wound infection or dehiscence and vaginal stenosis. Endoscopic treatment of neoplastic lesions is routinely used in human patients. However, the information about its use in small animals is sparse. Thus, the aim of the current study was to report two cases of successful vaginoscopic treatment of vaginal neoplasms in bitches. Case: A female Labrador weighing 26 kg (patient 1) and a mongrel bitch weighting 10 kg (patient 2) were attended due to vaginal bleeding. Physical examination revealed a pendunculated hard nodular mass in the caudal third of the vaginal dorsal fl oor in patient 1. In patient 2, two nodular, fi brous, infi ltrated masses of different dimension were touched on the mucosa of the caudal third of the vagina. The vaginal cytology revealed erythrocytes, cellular debris and anestrus in both cases. Given the clinical suspicion of vaginal neoplasia, the endoscopic approach by vaginoscopy was chosen in order for diagnostic investigation and surgical treatment. Under general anesthesia, a rigid 10-mm telescope with 6-mm working channel was employed for initial examination. A 10-mmHg CO2 pneumovagina was...(AU)

Resection of vaginal neoplasms by video-vaginoscopy in bitches

Background: Vaginal neoplasms usually represent a challenge for veterinary surgeons. Surgical resection often requires episiotomy or even laparotomy and pubic osteotomy, increasing the risk for intra and postoperative complications, such as severe pain, bleeding, wound infection or dehiscence and vaginal stenosis. Endoscopic treatment of neoplastic lesions is routinely used in human patients. However, the information about its use in small animals is sparse. Thus, the aim of the current study was to report two cases of successful vaginoscopic treatment of vaginal neoplasms in bitches. Case: A female Labrador weighing 26 kg (patient 1) and a mongrel bitch weighting 10 kg (patient 2) were attended due to vaginal bleeding. Physical examination revealed a pendunculated hard nodular mass in the caudal third of the vaginal dorsal fl oor in patient 1. In patient 2, two nodular, fi brous, infi ltrated masses of different dimension were touched on the mucosa of the caudal third of the vagina. The vaginal cytology revealed erythrocytes, cellular debris and anestrus in both cases. Given the clinical suspicion of vaginal neoplasia, the endoscopic approach by vaginoscopy was chosen in order for diagnostic investigation and surgical treatment. Under general anesthesia, a rigid 10-mm telescope with 6-mm working channel was employed for initial examination. A 10-mmHg CO2 pneumovagina was...

Congelação de espermatozoides epididimários de gatos utilizando o diluidor Botu-crio® após refrigeração por 24h em contêiner de transporte de sêmen Botu-tainer® / Cryopreservation of feline epididymal sperm using Botu-crio®extender after cooling for24h in Botu-tainer®semen transportation container

As possibilidades de uso de espermatozoides obtidos do epidídimo vêm sendo amplamente utilizadas devido à permanência da capacidade espermática fecundante e possibilidade de uso para felídeos selvagens. Porém, no processo de criopreservação, alguns estudos demonstram perdas na qualidade dos espermatozoides quando deixados sob refrigeração antes da congelação por tempos determinados. O presente trabalho teve por objetivo, avaliar a qualidade dos espermatozoides obtidos de epidídimos de gatos domésticos, após a criopreservação utilizando um meio diluente a base de gema de ovo com glicerol (Botu-crio®), comparando as características morfofuncionais, após refrigeração por 24 horas em container de transporte de sêmen (Botu-tainer®). Foram utilizados oito gatos domésticos submetidos à orquiectomia eletiva, com idades a partir de oito meses, sem determinação racial e em bom estado nutricional (2,5 ­5,5kg ou 4kg em média). As características espermáticas avaliadas foram: motilidade, vigor, concentração, integridade de membrana e morfologia espermática. Verificou-se que, após avaliações estatísticas, o container de transporte de sêmen foi capaz de manter a viabilidade espermática, mesmo após as 24h. Observou-se também uma queda significativa de todos os parâmetros pós-congelação, consequentes, provavelmente, ao estresse térmico que ocorre no processamento. No entanto, a porcentagem de integridade de membrana pós-descongelação demonstra boa empregabilidade do diluente Botu-crio®, com possível viabilidade in vitro para realização de fertilizações, necessitando de maiores avaliações.

The possibilities of using the sperm collected from the epididymis have been widely used because the fertilizing capacity sperm preservation and the possibility of using it for wild cats. But in the process of cryopreservation, some studies show a decrease in the quality of the sperm when left under cooling before frozen for some time. This study aimed to assess the quality of the epididymal sperm obtained from domestic cats after cryopreservation using a diluent based on egg yolkand glycerol (Botu-crio®), comparing the morphofunctional characteristics after cooling for 24 hours in a container of semen transport (Botu-tainer®). We use eight cats submitted to elective orchiectomy, aging from eight months, without racial determination, and good nutritional status. These sperm characteristics were: motility, vigor, concentration, membrane integrity and morphology. It has been found, after statistical analysis, that the container of semen was able to maintain sperm viability, even for 24h. We also observed a significant decrease on all parameters after frozen, consequential, probably to thermal stress that occurs in processing. However, the percentage of membrane integrity after thawing shows good employability of the Botu-crio®, which viability is possible to perform in vitro fertilization, requiring higher ratings.

Tratamento de papilomatose oral canina - relato de caso / Treatment for canine oral papillomatosis – a case report / Tratamiento de la papilomatosis oral canina – reporte de un caso

A papilomatose oral canina é uma enfermidade tumoral benigna, caracterizada pelo aparecimento de lesões múltiplas na região dos lábios, palato, faringe, esôfago, língua e eventualmente em outras regiões do corpo. A transmissão ocorre pelo contato direto ou indireto dos cães com secreções ou sangue provenientes dos papilomas. Em animais jovens, costuma apresentar evolução autolimitante, contudo, em alguns casos, pode se tornar crônica. O tratamento é incerto. Vários fármacos e métodos são descritos na terapia, porém não existe nenhum tratamento totalmente eficiente. O objetivo deste relato é documentar um caso de papilomatose oral em um cão da raça dálmata, de dois anos, que já havia passado por algumas tentativas de tratamento sem sucesso, sendo empregada uma alternativa de protocolo terapêutico, com a associação de um imunomodulador (Propionibacterium acnes) e um antineoplásico (Clorobutanol), com a qual se obteve sucesso.

The canine oral papilloma (oral warts) is a benign tumor disease, characterized by the emergence of multiple lesions at the lips, palate, pharynx, esophagus, tongue and eventually to others parts of the body. Transmission occurs by direct or indirect contact with secretions or blood from papillomas. Evolution is predominantly self-limiting in young animals, but can become chronic in some cases. Treatment options are unclear. Although several drugs and methods are described, none is highly efficient. The aim of this article is to report a case of oral papilloma in a two-year-old Dalmatian that had been previously treated with various protocols without success. Remission of symptoms was obtained by associating an immunomodulator (Propionibacterium acnes) with an antineoplastic (Chlorobutanol).

La papilomatosis oral canina es una enfermedad tumoral benigna que se caracteriza por la aparición de lesiones en áreas como labios, paladar, faringe, esófago, lengua y eventualmente, en otras regiones del cuerpo. La transmisión se da por contacto directo o indirecto a partir de perros con secreciones o sangre provenientes de los papilomas. En animales jóvenes suele tener una evolución autolimitante, a pesar de que en algunos casos la enfermedad puede hacerse crónica. El tratamiento comunmente genera dudas. Se han descripto varios fármacos y métodos alternativos para tratar esta enfermedad, pero aún no existe una terapéutica totalmente eficiente. El objetivo del presente trabajo es documentar un caso de papilomatosis oral en un perro Dálmata de dos años, que habiendo recibido varios tratamientos sin éxito, finalmente se le indicó una asociación de un inmunomodulador (Propionibacterium acnes) y de un antineoplásico (Clorobutanol), con los que se obtuvieron buenos resultados terapéuticos.

Tratamento de papilomatose oral canina - relato de caso / Treatment for canine oral papillomatosis a case report / Tratamiento de la papilomatosis oral canina reporte de un caso

A papilomatose oral canina é uma enfermidade tumoral benigna, caracterizada pelo aparecimento de lesões múltiplas na região dos lábios, palato, faringe, esôfago, língua e eventualmente em outras regiões do corpo. A transmissão ocorre pelo contato direto ou indireto dos cães com secreções ou sangue provenientes dos papilomas. Em animais jovens, costuma apresentar evolução autolimitante, contudo, em alguns casos, pode se tornar crônica. O tratamento é incerto. Vários fármacos e métodos são descritos na terapia, porém não existe nenhum tratamento totalmente eficiente. O objetivo deste relato é documentar um caso de papilomatose oral em um cão da raça dálmata, de dois anos, que já havia passado por algumas tentativas de tratamento sem sucesso, sendo empregada uma alternativa de protocolo terapêutico, com a associação de um imunomodulador (Propionibacterium acnes) e um antineoplásico (Clorobutanol), com a qual se obteve sucesso.(AU)

The canine oral papilloma (oral warts) is a benign tumor disease, characterized by the emergence of multiple lesions at the lips, palate, pharynx, esophagus, tongue and eventually to others parts of the body. Transmission occurs by direct or indirect contact with secretions or blood from papillomas. Evolution is predominantly self-limiting in young animals, but can become chronic in some cases. Treatment options are unclear. Although several drugs and methods are described, none is highly efficient. The aim of this article is to report a case of oral papilloma in a two-year-old Dalmatian that had been previously treated with various protocols without success. Remission of symptoms was obtained by associating an immunomodulator (Propionibacterium acnes) with an antineoplastic (Chlorobutanol).(AU)

La papilomatosis oral canina es una enfermedad tumoral benigna que se caracteriza por la aparición de lesiones en áreas como labios, paladar, faringe, esófago, lengua y eventualmente, en otras regiones del cuerpo. La transmisión se da por contacto directo o indirecto a partir de perros con secreciones o sangre provenientes de los papilomas. En animales jóvenes suele tener una evolución autolimitante, a pesar de que en algunos casos la enfermedad puede hacerse crónica. El tratamiento comunmente genera dudas. Se han descripto varios fármacos y métodos alternativos para tratar esta enfermedad, pero aún no existe una terapéutica totalmente eficiente. El objetivo del presente trabajo es documentar un caso de papilomatosis oral en un perro Dálmata de dos años, que habiendo recibido varios tratamientos sin éxito, finalmente se le indicó una asociación de un inmunomodulador (Propionibacterium acnes) y de un antineoplásico (Clorobutanol), con los que se obtuvieron buenos resultados terapéuticos.(AU)