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1.
Rev. Bras. Parasitol. Vet. (Online) ; 32(1): e012222, 2023. ilus, tab, mapas
Artigo em Inglês | VETINDEX | ID: biblio-1416495

Resumo

Diagnosis is crucial for controlling giardiasis. We determined the prevalence and genetically characterize isolates of Giardia duodenalis of children and dogs from rural communities in northeastern Brazil. G. duodenalis cysts were concentrated by centrifugal flotation/sedimentation. Molecular characterization was carried out using the loci ssu-rRNA, bg, tpi, and gdh. By parasitological techniques, Giardia spp. infection was detected in 72/192 children (37.5%; 95% CI: 30.6%-44.7%) and 24/139 dogs (17.3%; 95% CI: 11.4%-24.6%). By molecular analysis, infection was detected in 60/141 children (42.5%; 95% CI: 34.3%-51.2%) and 26/92 dogs (28.3%; 95% CI: 19.4%-38.6%). The total prevalence of giardiasis was 54.9% in children (106/193; 95% CI: 47.1%-61.6%) and 32.9% in dogs (47/143; 95% CI: 25.2%-41.2%). Zoonotic assemblages A and B of G. duodenalis were detected in children, and assemblage E of G. duodenalis was detected in one child and two dogs. Parallel use of parasitological and molecular techniques proved to be a more effective strategy for detecting giardiasis in children and dogs from endemic areas.(AU)


O diagnóstico é crucial para o controle da giardíase. Foram determinadas as prevalências e as características genéticas isoladas de Giardia duodenalis, em crianças e cães de comunidades rurais do nordeste brasileiro. Os cistos de G. duodenalis foram concentrados por centrífugo-flutuação/sedimentação. A caracterização molecular foi realizada utilizando-se os loci ssu-rRNA, bg, tpi e gdh. Pelas técnicas parasitológicas, a infecção por Giardia spp. foi detectada em 72/192 crianças (37,5%; IC 95%: 30,6%-44,7%) e 24/139 cães (17,3%; IC 95%: 11,4%-24,6%). Molecularmente, a infecção foi detectada em 60/141 crianças (42,5%; IC 95%: 34,3%-51,2%) e 26/92 cães (28,3%; IC 95%: 19,4%-38,6%). A prevalência total de giardíase foi de 54,9% em crianças (106/193; IC 95%: 47,1%-61,6%) e 32,9% em cães (47/143; IC 95%: 25,2%-41,2%). Os assemblages zoonóticos A e B de G. duodenalis foram detectados em crianças, e o assemblage E de G. duodenalis foi detectado nas duas populações. O uso paralelo de técnicas parasitológicas e moleculares mostra-se uma estratégia mais eficaz para detecção de giardíase em crianças e cães de áreas endêmicas.(AU)


Assuntos
Humanos , Animais , Criança , Cães , Doenças Parasitárias/diagnóstico , Giardíase/diagnóstico , Brasil
2.
Acta sci. vet. (Impr.) ; 45: 01-09, 2017. ilus, map, tab
Artigo em Inglês | VETINDEX | ID: biblio-1457594

Resumo

Background: Tickborne diseases are frequent in tropical countries such as Brazil. Protozoa of the Babesia genus and bacteria of the Ehrlichia genus spread throughout the country with high prevalences in urban and rural areas, causing clinical or subclinical diseases in dogs. This study aimed to investigate the prevalence of infection from Babesia spp. and Ehrlichia chaffeensis in the dog population in the municipality of Ituberá, Bahia, Brazil, and to verify the risk factors associated with the infections. Materials, Methods & Results: A cross-sectional study was conducted, consisting of the following procedures: clinical examination and blood samples collection from 380 dogs and application of a structure questionnaire to dog owners to collect epidemiological data. All dogs were evaluated for the presence of ticks and clinical signs associated with the infections. Blood samples were collected and tested for Babesia spp. through capillary blood smears, indirect immunofluorescence assays (IFAT), and polymerase chain reaction (PCR); all the samples were also tested for E. chaffeensis through nested PCR. Intra-erythrocyte piroplasms were visualized in the blood smears of two animals (2/380; 0.5%) in the cytology exams. Anti-B. canis antibodies were detected in 140/380 (36.8%) dogs, at 1:40 dilution. By PCR, 147/380 (38.7%) dogs tested positive for infection by Babesia sp., but no animal was infected by E. chaffeensis. Only 115/380 dogs (30.3%) were infested by ticks. In total, 223/380 dogs (58.7%) were found infected by Babesia spp. No clinical signs were it found to be significant for the infection. The infected (Ht = 40%) and uninfected dogs’ (Ht = 39%) hematocrit averages were not found to significantly differ (P = 0.47). No hematological changes were found to be significant for the disease. The evaluated variables sex, habitat (urban or rural) […]


Assuntos
Animais , Cães , Babesia/isolamento & purificação , Babesiose/epidemiologia , Ehrlichia chaffeensis/isolamento & purificação , Ehrlichiose/epidemiologia , Ehrlichiose/veterinária , Reação em Cadeia da Polimerase/veterinária , Técnica Indireta de Fluorescência para Anticorpo/veterinária
3.
Acta sci. vet. (Online) ; 45: 01-09, 2017. ilus, mapas, tab
Artigo em Inglês | VETINDEX | ID: vti-16398

Resumo

Background: Tickborne diseases are frequent in tropical countries such as Brazil. Protozoa of the Babesia genus and bacteria of the Ehrlichia genus spread throughout the country with high prevalences in urban and rural areas, causing clinical or subclinical diseases in dogs. This study aimed to investigate the prevalence of infection from Babesia spp. and Ehrlichia chaffeensis in the dog population in the municipality of Ituberá, Bahia, Brazil, and to verify the risk factors associated with the infections. Materials, Methods & Results: A cross-sectional study was conducted, consisting of the following procedures: clinical examination and blood samples collection from 380 dogs and application of a structure questionnaire to dog owners to collect epidemiological data. All dogs were evaluated for the presence of ticks and clinical signs associated with the infections. Blood samples were collected and tested for Babesia spp. through capillary blood smears, indirect immunofluorescence assays (IFAT), and polymerase chain reaction (PCR); all the samples were also tested for E. chaffeensis through nested PCR. Intra-erythrocyte piroplasms were visualized in the blood smears of two animals (2/380; 0.5%) in the cytology exams. Anti-B. canis antibodies were detected in 140/380 (36.8%) dogs, at 1:40 dilution. By PCR, 147/380 (38.7%) dogs tested positive for infection by Babesia sp., but no animal was infected by E. chaffeensis. Only 115/380 dogs (30.3%) were infested by ticks. In total, 223/380 dogs (58.7%) were found infected by Babesia spp. No clinical signs were it found to be significant for the infection. The infected (Ht = 40%) and uninfected dogs (Ht = 39%) hematocrit averages were not found to significantly differ (P = 0.47). No hematological changes were found to be significant for the disease. The evaluated variables sex, habitat (urban or rural) […](AU)


Assuntos
Animais , Cães , Babesia/isolamento & purificação , Ehrlichia chaffeensis/isolamento & purificação , Ehrlichiose/epidemiologia , Ehrlichiose/veterinária , Babesiose/epidemiologia , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Reação em Cadeia da Polimerase/veterinária
4.
Acta sci. vet. (Online) ; 43(supl): 1-6, Aug. 14, 2015. tab
Artigo em Inglês | VETINDEX | ID: vti-716878

Resumo

Background: Primary hypoparathyroidism (PH) is an endocrine disorder characterized by decreased production and/or release of parathyroid hormone (PTH). Dogs and cats are rarely affected, and typical clinical signs include an abrupt onset of neurological and neuromuscular signs. The diagnosis is based on the history, clinical signs, and laboratory findings of hypocalcaemia, as well as the exclusion of other causes of tetany. Treatment involves stabilization of serum calcium with specific therapy. This study aimed to report three cases of PH: two canine cases and the first feline case in Brazil. Cases: 1) A 8-year-old male Yorkshire terrier was brought to the clinic with a history of tetanic crisis. The owner reported that the animal had been previously diagnosed with epilepsy and since treated with phenobarbital. The physical examination revealed hyperthermia, tachypnea, tachycardia, salivation, and ataxia with hyperextension of the anterior and posterior limbs. Emergency treatment included intravenous (IV) administration of calcium gluconate, and the animal showed clinical improvement within an hour. Overall, the complete blood count was within the normal range, but the calcium and PTH levels were below the normal ranges. Therefore, PH was confirmed. Calcium carbonate and vitamin D3 supplementation were prescribed, and the treatment with phenobarbital was suspended. During the...(AU)


Assuntos
Animais , Gatos , Cães , Hipoparatireoidismo/veterinária , Doenças das Paratireoides/veterinária , Distúrbios do Metabolismo do Cálcio/veterinária , Doenças do Sistema Endócrino/veterinária , Tetania/veterinária
5.
Acta sci. vet. (Impr.) ; 43(supl): 1-6, Aug. 14, 2015. tab
Artigo em Inglês | VETINDEX | ID: biblio-1457373

Resumo

Background: Primary hypoparathyroidism (PH) is an endocrine disorder characterized by decreased production and/or release of parathyroid hormone (PTH). Dogs and cats are rarely affected, and typical clinical signs include an abrupt onset of neurological and neuromuscular signs. The diagnosis is based on the history, clinical signs, and laboratory findings of hypocalcaemia, as well as the exclusion of other causes of tetany. Treatment involves stabilization of serum calcium with specific therapy. This study aimed to report three cases of PH: two canine cases and the first feline case in Brazil. Cases: 1) A 8-year-old male Yorkshire terrier was brought to the clinic with a history of tetanic crisis. The owner reported that the animal had been previously diagnosed with epilepsy and since treated with phenobarbital. The physical examination revealed hyperthermia, tachypnea, tachycardia, salivation, and ataxia with hyperextension of the anterior and posterior limbs. Emergency treatment included intravenous (IV) administration of calcium gluconate, and the animal showed clinical improvement within an hour. Overall, the complete blood count was within the normal range, but the calcium and PTH levels were below the normal ranges. Therefore, PH was confirmed. Calcium carbonate and vitamin D3 supplementation were prescribed, and the treatment with phenobarbital was suspended. During the...


Assuntos
Animais , Gatos , Cães , Distúrbios do Metabolismo do Cálcio/veterinária , Doenças das Paratireoides/veterinária , Hipoparatireoidismo/veterinária , Doenças do Sistema Endócrino/veterinária , Tetania/veterinária
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