Construction and characterization of a recombinant vaccine encoding the nucleocapsid protein gene of avian infectious bronchitis virus / Construção e caracterização de uma vacina recombinante codificando o gene da proteina do nucleocapsideo do virus da bronquite infecciosa das galinhas

A vacinação é a forma mais utilizada para prevenir a bronquite infecciosa causada pelo vírus da bronquite infecciosa das galinhas (IBV). Contudo, as vacinas convencionais são incapazes de diferenciar aves infectadas de vacinadas. No presente trabalho foi construído, caracterizado, e avaliado como candidato vacinal, um adenovírus recombinante expressando o gene N do IBV. O gene N foi clonado em um adenovírus humano tipo 5 defectivo e transfectado para as células HEK-293A para gerar rAd5_N. Após o vetor ser obtido como esperado e a confirmação da expressão da proteína N em HEK-293ª, foi realizada inoculação pela via oculo-nasal na dose de 10 7 TCID 50 /0,1mL para imunização de galinhas livres de patógenos específicos (SPF). A resposta imunológica do Ad5_N e a proteção contra o desafio ao IBV foram avaliadas e comparadas com uma vacina viva comercial. Não foram detectados anticorpos anti-IBV em aves vacinadas com o Ad5_N. A vacina comercial induziu anticorpos detectáveis a partir do 7º dia pós-vacinal. Em aves vacinadas com o Ad5_N não houve aumento na expressão de IFNγ. Neste estudo, o rAd5_N obtido não conferiu proteção contra desafio com IBV-M41. Os resultados indicam a necessidade de avaliar adenovírus recombinantes expressando outros genes do IBV.(AU)

Construction and characterization of a recombinant vaccine encoding the nucleocapsid protein gene of avian infectious bronchitis virus / Construção e caracterização de uma vacina recombinante codificando o gene da proteina do nucleocapsideo do virus da bronquite infecciosa das galinhas

A vacinação é a forma mais utilizada para prevenir a bronquite infecciosa causada pelo vírus da bronquite infecciosa das galinhas (IBV). Contudo, as vacinas convencionais são incapazes de diferenciar aves infectadas de vacinadas. No presente trabalho foi construído, caracterizado, e avaliado como candidato vacinal, um adenovírus recombinante expressando o gene N do IBV. O gene N foi clonado em um adenovírus humano tipo 5 defectivo e transfectado para as células HEK-293A para gerar rAd5_N. Após o vetor ser obtido como esperado e a confirmação da expressão da proteína N em HEK-293ª, foi realizada inoculação pela via oculo-nasal na dose de 10 7 TCID 50 /0,1mL para imunização de galinhas livres de patógenos específicos (SPF). A resposta imunológica do Ad5_N e a proteção contra o desafio ao IBV foram avaliadas e comparadas com uma vacina viva comercial. Não foram detectados anticorpos anti-IBV em aves vacinadas com o Ad5_N. A vacina comercial induziu anticorpos detectáveis a partir do 7º dia pós-vacinal. Em aves vacinadas com o Ad5_N não houve aumento na expressão de IFNγ. Neste estudo, o rAd5_N obtido não conferiu proteção contra desafio com IBV-M41. Os resultados indicam a necessidade de avaliar adenovírus recombinantes expressando outros genes do IBV.(AU)

Effect of floor type (dirt or concrete) on litter quality, house environmental conditions, and performance of broilers

This study was conducted with the objective of evaluating the use of concrete or hard-packed dirt floor in broiler houses. This experiment was carried out in two different phases. The following performance parameters were studied: live weight, feed intake, feed conversion ratio, and mortality. Litter moisture, pH and temperature were measured. Litter residual contamination after cleaning and disinfection was also evaluated. A dry bulb thermometer, a wet bulb thermometer, and a black bulb thermometer were placed inside each broiler house at bird height and outside the broiler house for data collection. Environmental data were collected at 3h intervals from 00:00 to 24:00 hours during weeks 4, 5, and 6 of the grow-out. Based on the collected data, air relative humidity (RH) was determined, after which wet bulb globe temperature (WBGT) and Radiant Heat Load (RHL) were calculated. There were no differences in live performance parameters. However, total mortality and sudden death were higher in birds raised on dirt floor. On days 0, 14 and 35, litter pH was higher in the dirt floor as compared to the concrete floor, but at the end of the grow-out, this difference disappeared. There was a cubic effect of bird age on litter moisture, which increased up to day 28, and then stabilized or decreased. Litter coliform contamination was higher at the end of the grow-out as compared to that found at housing, but it was not influenced by floor type. The general thermal comfort of broiler raised on dirt floor was similar to that of broilers raised on concrete floor.(AU)

Effect of floor type (dirt or concrete) on litter quality, house environmental conditions, and performance of broilers

This study was conducted with the objective of evaluating the use of concrete or hard-packed dirt floor in broiler houses. This experiment was carried out in two different phases. The following performance parameters were studied: live weight, feed intake, feed conversion ratio, and mortality. Litter moisture, pH and temperature were measured. Litter residual contamination after cleaning and disinfection was also evaluated. A dry bulb thermometer, a wet bulb thermometer, and a black bulb thermometer were placed inside each broiler house at bird height and outside the broiler house for data collection. Environmental data were collected at 3h intervals from 00:00 to 24:00 hours during weeks 4, 5, and 6 of the grow-out. Based on the collected data, air relative humidity (RH) was determined, after which wet bulb globe temperature (WBGT) and Radiant Heat Load (RHL) were calculated. There were no differences in live performance parameters. However, total mortality and sudden death were higher in birds raised on dirt floor. On days 0, 14 and 35, litter pH was higher in the dirt floor as compared to the concrete floor, but at the end of the grow-out, this difference disappeared. There was a cubic effect of bird age on litter moisture, which increased up to day 28, and then stabilized or decreased. Litter coliform contamination was higher at the end of the grow-out as compared to that found at housing, but it was not influenced by floor type. The general thermal comfort of broiler raised on dirt floor was similar to that of broilers raised on concrete floor.

Condições térmicas ambientais e desempenho de aves criadas em aviários com e sem o uso de forro / Environmental thermal conditions and performance of broilers raised in aviaries with and without the use of polyethylene lining

O experimento foi realizado em delineamento inteiramente ao acaso, em esquema fatorial 2x2, para avaliar o efeito de época de ano, o tipo de forro de polietileno sobre as condições térmicas, de umidade e o desempenho de aves de corte. Foram utilizados quatro aviários, cada um dividido internamente em quatro boxes para alojar 200 aves cada. As variáveis de desempenho avaliadas foram peso vivo, ganho de peso, consumo de ração, conversão alimentar e mortalidade das aves do nascimento ao 42º dia de idade. O desempenho das aves foi melhor com aviários com forro. As condições térmicas foram melhoradas cm a utilização de forro nos aviários(AU)

This experiment was carried out in a completely randomized experimental design, factorial arrangement 2x2, to evaluate the effect of period of year and type polyethylene liming on thermal and humidity of aviaries and performance of broiler. Four aviaries were divided in four boxes to house 200 chickens each. The recorded performance traits were body weight, weight gain feed intake, feed:weght gain ratio and mortality from hatch to 42 days of age. Better thermal conditions and higher chicken performance were observed in the polyethylene liming aviaries(AU)