Topical application of gentamicin or cephalothin in the healing of surgical wounds in dogs / Aplicação tópica de gentamicina ou cefalotina na cicatrização de feridas cirúrgicas em cães

The present study aimed to evaluate the influence of local administration of gentamicin or cephalothin on the healing of surgical wounds in dogs. Thirty healthy dogs were submitted to surgical castration. They were divided into 2 groups of 15 animals. After the surgical procedure, two 0.6 cm circular cutaneous wounds were made in the abdominal region. Group 1 received 0.1 ml of 0.9% saline in one wound and 0.1 ml of gentamicin (40 mg / ml) in the other wound; and group 2 received 0.1 ml of 0.9% saline and 0.1 ml of cephalothin (200 mg / ml); respectively. All wounds were sutured and assessed macroscopically 1, 3 and 10 days after the operation. On day 10, an incisional biopsy was performed for histopathological evaluation. All analyzes of macroscopic variables did not show significant differences between groups (P <0.05). There were no significant differences between groups for microscopic evaluation of collagenization, vascularization, edema and inflammatory cells (P <0.05). Thus, the topical use of the tested antibiotics does not influence skin healing in dogs.

O presente estudo teve como objetivo avaliar a influência da administração local de gentamicina ou cefalotina na cicatrização de feridas cirúrgicas em cães. Trinta cães saudáveis foram submetidos à cirurgia de castração. Eles foram divididos em 2 grupos de 15 animais. Após o procedimento cirúrgico, foram feitas duas feridas cutâneas circulares de 0,6 cm na região abdominal. O grupo 1 recebeu 0,1 ml de soro fisiológico 0,9% em uma ferida e 0,1 ml de gentamicina (40 mg/ml) na outra ferida; e o grupo 2 recebeu 0,1 ml de soro fisiológico 0,9% e 0,1 ml de cefalotina (200 mg/ml); respectivamente. Todas as feridas foram suturadas e avaliadas macroscopicamente 1, 3 e 10 dias após a operação. No dia 10, foi realizada biópsia incisional para avaliação histopatológica. Todas as análises das variáveis macroscópicas não mostraram diferenças significativas entre os grupos (P<0,05). Não houve diferenças significativas entre os grupos para avaliação microscópica da colagenização, vascularização, edema e células inflamatórias (P<0,05). Assim, o uso tópico dos antibióticos testados não influencia na cicatrização da pele em cães.

Sinfisiodese púbica juvenil associada à miectomia do pectíneo para tratamento de displasia coxofemoral em cão / Juvenile pubic symphysiodesis associated with pectineus myectomy for treatment of hip dysplasia in dog

Background: Hip dysplasia (HD) is the most common non-traumatic orthopedic disease in large breed dogs. Treatment is chosen according to the severity of the condition, the age of the dog and the involvement of osteoarthritis (OA). For puppies up to 20-weeks-old, surgical treatment by the juvenile pubic symphysiodesis (JPS) technique can be performed. This procedure promotes the ventrolateral rotation of the acetabulum over the femoral head, which improves hip joint congruence and stability. The objective of this study is to report the case of a young dog with HD treated with JPS and pectineus myectomy. Clinical and radiographic outcomes were assessed for up to one year after surgery. Case: A 17-week-old Saint Bernard puppy was attended at the University Veterinary Hospital showing abnormal weight bearing and lameness in pelvic limbs, without history of previous trauma. On orthopedic examination, there was a marked hypotrophy of the pelvic limb muscles, severe pain and laxity in the hip joints, and positive feature in the Ortolani test. The hip joint subluxation angle (SA) and reduction angle (RA) were measured. A radiographic study was carried out to evaluate the hip joints and measure the Norberg angle (NA), distraction index (DI), acetabular angle (AA) and dorsal acetabular rim angle (DARA). The 20-week-old dog underwent surgical treatment by JPS technique. A ventral surgical approach to the pubis was performed and the pubic symphysis was cauterized with electrocautery. Partial pectineus myectomy technique was also performed. Drug therapy and restriction of physical activities were indicated in the early postoperative. The dog was evaluated by clinical and radiographic examinations at 4 months and 1 year after the surgical procedure. In the reassessment after 4 months of surgical treatment, the patient showed improvement in clinical signs, with mild lameness and absence of painful sensitivity in the movement of the hip joints. Radiographically, hip joint incongruity was observed, but with progressive improvement in the values of the measured variables. In the clinical examination 1 year after the procedure, the dog showed satisfactory weight bearing with slight lameness in pelvic limbs in the running gait. There was improvement in the thigh muscles and no painful signs were observed in the hip joints, but a positive result was detected in the Ortolani test. In the radiographic examination, bilateral articular incongruity was still observed, however, there was a mild improvement in the coverage of the acetabulum over the femoral head. The measured angles and indices showed favorable results regarding the recovery of hip joint stability and proper development. Discussion: Studies evaluating the JPS technique for the treatment of HD have shown to be a relatively simple and effective procedure, which allows altering the acetabular coverage, reducing the development of HD and the progression of OA. The need for an early diagnosis of this condition is essential, so that the JPS technique can be used in young dogs and offer effective results. In the present report, in the late postoperative period, the dog showed improvement in clinical signs, with favorable weight bearing and ambulation in pelvic limbs, recovery of limb muscles, absence of pain in the hip joints and decrease in RA and SA. Radiographically, the reduction in joint subluxation, improvement in acetabular coverage over femoral heads, increase in NA and AA, reduction in DI and DARA are evidence of the favorable outcome of ventrolateral rotation of the acetabulum after surgical treatment. The surgical technique used was effective, which enabled the dog to recover the functional use of the pelvic limbs and improve the quality of life.

Biomechanical evaluation of a new femoral stem design for total hip replacement in a canine model

ABSTRACT Purpose To evaluate the biomechanical properties of a novel total hip replacement femoral stem. Methods Eight pairs of femurs from dog cadavers were used. The femurs were separated into different groups. A novel femoral stem with a convex proximal portion (Stem B) was biomechanically evaluated and compared to awell-known veterinary collared stem (Stem A). Femoral stems were inserted into the contralateral femurs from the same dog, forming 16 constructs. A flexo-compression load was applied on the axial axis of each sample. Maximum strength, deflection, stiffness, and energy absorption were analysed. Results Group B constructs showed significantly higher values (p ? 0.05) for the variables, except stiffness. The mean maximum strength was 1,347 ± 357 N for Group A and 1,805 ± 123 N for Group B (p ? 0.0069). The mean deflection was5.54 ± 2.63 mm for Group A and 10.03 ± 3.99 mm for Group B (p ? 0.0056). For the energy variable, the force was 6,203 ± 3,488 N/mm for Group A and 12,885 ± 5,056 N/mm for Group B (p ? 0.0054). Stem B had greater maximum strength, deflection, and energy. Conclusions The new stem was effective in neutralizing the impact of axial flexion-compression stresses during biomechanical tests in cadaveric models.

Topical application of lidocaine or bupivacaine in the healing of surgical wounds in dogs

Purpose: To analyze the anesthetic drugs interference with wound healing when used in the surgical bed. Methods: Macro and microscopic aspects of healing of surgical wounds were evaluated after instillation of topical anesthetics without vasoconstrictor or saline solution 0.9% as control in the transsurgical period. Thirty dogs, males and females were divided into two experimental groups. In both groups, two circular punch lesions of 6 mm diameter were performed in the abdomen. In group 1, lidocaine was instilled in one of the lesions and saline solution in the contralateral lesion. In group 2 the procedure was repeated with the use of bupivacaine. The macroscopic assessment of the lesions was performed on the first, third and tenth postoperative day. The excisional biopsy was performed on the tenth day and the samples were submitted for histopathological examination. Results: The macroscopic analysis had a significant difference between groups. Microscopic analysis was not significant between groups. Conclusions: The topical application of lidocaine and bupivacaine in the surgical wound is feasible and it does not influence skin healing. The benefit of such a practice, which has been the subject of other studies, seems to outweigh the risks.(AU)

Association of rush pin and intramedular pin techniques for the stabilization of salter harris type I fracture in rabbit (Oryctolagus cuniculus)

Background: In domestic rabbits, fractures are usually the result of household accidents. Fractures of the distal femoral physis are frequently observed in animals with immature skeletons and may cause future orthopedic problems. With this type of fracture, early reduction and stabilization are necessary to prevent additional damage to the physis and to preserve the growth potential of the bone. This report aims to describe the clinical and radiographic findings, as well as the surgical method used that combined Rush pins with an intramedullary pin, associated with a Salter Harris type I fracture of the right distal femur in a rabbit.Case: A 4-month male domestic rabbit (Oryctolagus cuniculus) weighing 1.2 kg, was referred to the Veterinary Hospital with a right pelvic limb lameness after an episode of trauma. Orthopedic examination revealed swelling, instability, crepitation, and increased pain sensitivity in the distal aspect of the right femur. Following physical and radiographic examination, a Salter Harris type I fracture of the right distal femur was diagnosed. The patient was premedicated with a combination of xylazine (2 mg/kg IM) and ketamine (10 mg/kg IM), which allowed placement of an IV catheter for the administration of fluids and intubation using an endotracheal tube with an internal diameter of 2 mm. Anesthesia was maintained with isoflurane, and an epidural was performed with a combination of lidocaine (2 mg/kg) and bupivacaine (0.75 mg/kg). Osteosynthesis was performed with two Rush pins and an intramedullary pin. Radiographic examination 110 and 330 days after the surgical procedure showed good alignment of the bone and adequate healing of the fracture. The combination of techniques used in this case report proved to be effective, resulting in functional recovery of the limb and rapid bone healing.[...](AU)

Association of rush pin and intramedular pin techniques for the stabilization of salter harris type I fracture in rabbit (Oryctolagus cuniculus)

Background: In domestic rabbits, fractures are usually the result of household accidents. Fractures of the distal femoral physis are frequently observed in animals with immature skeletons and may cause future orthopedic problems. With this type of fracture, early reduction and stabilization are necessary to prevent additional damage to the physis and to preserve the growth potential of the bone. This report aims to describe the clinical and radiographic findings, as well as the surgical method used that combined Rush pins with an intramedullary pin, associated with a Salter Harris type I fracture of the right distal femur in a rabbit.Case: A 4-month male domestic rabbit (Oryctolagus cuniculus) weighing 1.2 kg, was referred to the Veterinary Hospital with a right pelvic limb lameness after an episode of trauma. Orthopedic examination revealed swelling, instability, crepitation, and increased pain sensitivity in the distal aspect of the right femur. Following physical and radiographic examination, a Salter Harris type I fracture of the right distal femur was diagnosed. The patient was premedicated with a combination of xylazine (2 mg/kg IM) and ketamine (10 mg/kg IM), which allowed placement of an IV catheter for the administration of fluids and intubation using an endotracheal tube with an internal diameter of 2 mm. Anesthesia was maintained with isoflurane, and an epidural was performed with a combination of lidocaine (2 mg/kg) and bupivacaine (0.75 mg/kg). Osteosynthesis was performed with two Rush pins and an intramedullary pin. Radiographic examination 110 and 330 days after the surgical procedure showed good alignment of the bone and adequate healing of the fracture. The combination of techniques used in this case report proved to be effective, resulting in functional recovery of the limb and rapid bone healing.[...]

Pantarsal arthrodesis with a customized titanium medial plate in a dog

Background: Tibiotarsal joint luxations are mostly traumatic and due to automobile accidents. The arthrodesis of this joint is considered the final solution for trauma with ligament lesions without repair, luxations without possibility of joint recovery and chronic joint diseases. The direction to which the joint is dislocated is associated with the forces of trauma, and bilateral ligament injuries are not uncommon. However, the medial ligaments are more frequently injured. The aim of this study is to describe the performance of pantarsal arthrodesis using a customized titanium plate for application to the medial surface of the joint.Case: An undefined breed dog, weighing 21.5 kg, was attended at the Veterinary Hospital of the Federal University of Lavras (UFLA) with history of trauma and injury in the right pelvic limb. After physical and radiographic examinations it was diagnosed with an exposed luxation of the tibiotarsal joint and tissue loss in the region. The tibiotarsal region was cleaned and a bandage was made daily until the first surgical procedure, which occurred five days later. Initially, it was treated without success with the use of a transarticular external fixator1 . Subsequently, pantarsal arthrodesis was performed using a customized titanium angled plate (Made by a private manufacturer based on the functional angulation of the tibiotarsal joint of the contralateral limb). The joint was accessed for exposure of the distal portion of the tibia, tarsal and metatarsals bones. Wear of the articular surfaces was performed and then the customized angulation plate for pantarsal arthrodesis was placed on the medial side of the joint. Bicortical drillings were performed, starting with the proximal orifice. According to the holes of the plate, five 3.5 mm diameter screws1 (four at the distal tibia and one at the tarsus) and four 2.7 mm diameter screws were used in the metatarsal bones.[...](AU)

Enxerto osteocondral autógeno associado ao IGF-1 emlesão induzida na cartilagem articular de coelhos / Autogenous osteochondral graft associated with IGF-1 in induced articular cartilage lesion in rabbits

Background: Articular cartilage has a limited capacity for regeneration and of the various treatments proposed, none have reached appropriate therapeutic effectiveness. This study aimed to evaluate autogenous osteochondral grafts in intact or macerated format, in association with or without insulin-like growth factor type-1 (IGF-1) in the repair of osteochondral defects induced in the femoral trochlear groove of rabbits.Materials, Methods & Results: Seventeen healthy White New Zealand rabbits were selected for this study. The rabbits were female, six months old, and had an average body weight of 4.5 kg. All 34 stifle joints were subjected to autogenous osteochondral grafting in the femoral trochlear groove. The joints were divided into four groups designated as intact osteochondral graft with IGF-1 (INT + IGF), intact osteochondral graft with physiological solution (INT + FIS), macerated osteochondral graft with IGF-1 (MAC + IGF), and macerated osteochondral graft with physiological solution (MAC + FIS). Serial evaluations were performed by orthopedic and radiographic examination. After 6 and 12 weeks postoperatively, the grafted area was subjected to macroscopic, histological, and immunohistochemical analyses. Although no statistically significant differences were found between the groups in relation to clinical, macroscopic, histological, and immunohistochemical aspects, a tendency of IGF-1 to promote tissue repair was evident. In the radiographic evaluation, the articular surface and the recipient site in both groups with IGF-1 showed significantly more effective filling (P ≤ 0.05). Regardless of the group, collagen type 2 production, as assessed by immunohistochemistry, was found to be appropriate on the grafted articular surface.[...]

Pantarsal arthrodesis with a customized titanium medial plate in a dog

Background: Tibiotarsal joint luxations are mostly traumatic and due to automobile accidents. The arthrodesis of this joint is considered the final solution for trauma with ligament lesions without repair, luxations without possibility of joint recovery and chronic joint diseases. The direction to which the joint is dislocated is associated with the forces of trauma, and bilateral ligament injuries are not uncommon. However, the medial ligaments are more frequently injured. The aim of this study is to describe the performance of pantarsal arthrodesis using a customized titanium plate for application to the medial surface of the joint.Case: An undefined breed dog, weighing 21.5 kg, was attended at the Veterinary Hospital of the Federal University of Lavras (UFLA) with history of trauma and injury in the right pelvic limb. After physical and radiographic examinations it was diagnosed with an exposed luxation of the tibiotarsal joint and tissue loss in the region. The tibiotarsal region was cleaned and a bandage was made daily until the first surgical procedure, which occurred five days later. Initially, it was treated without success with the use of a transarticular external fixator1 . Subsequently, pantarsal arthrodesis was performed using a customized titanium angled plate (Made by a private manufacturer based on the functional angulation of the tibiotarsal joint of the contralateral limb). The joint was accessed for exposure of the distal portion of the tibia, tarsal and metatarsals bones. Wear of the articular surfaces was performed and then the customized angulation plate for pantarsal arthrodesis was placed on the medial side of the joint. Bicortical drillings were performed, starting with the proximal orifice. According to the holes of the plate, five 3.5 mm diameter screws1 (four at the distal tibia and one at the tarsus) and four 2.7 mm diameter screws were used in the metatarsal bones.[...]

Enxerto osteocondral autógeno associado ao IGF-1 emlesão induzida na cartilagem articular de coelhos / Autogenous osteochondral graft associated with IGF-1 in induced articular cartilage lesion in rabbits

Background: Articular cartilage has a limited capacity for regeneration and of the various treatments proposed, none have reached appropriate therapeutic effectiveness. This study aimed to evaluate autogenous osteochondral grafts in intact or macerated format, in association with or without insulin-like growth factor type-1 (IGF-1) in the repair of osteochondral defects induced in the femoral trochlear groove of rabbits.Materials, Methods & Results: Seventeen healthy White New Zealand rabbits were selected for this study. The rabbits were female, six months old, and had an average body weight of 4.5 kg. All 34 stifle joints were subjected to autogenous osteochondral grafting in the femoral trochlear groove. The joints were divided into four groups designated as intact osteochondral graft with IGF-1 (INT + IGF), intact osteochondral graft with physiological solution (INT + FIS), macerated osteochondral graft with IGF-1 (MAC + IGF), and macerated osteochondral graft with physiological solution (MAC + FIS). Serial evaluations were performed by orthopedic and radiographic examination. After 6 and 12 weeks postoperatively, the grafted area was subjected to macroscopic, histological, and immunohistochemical analyses. Although no statistically significant differences were found between the groups in relation to clinical, macroscopic, histological, and immunohistochemical aspects, a tendency of IGF-1 to promote tissue repair was evident. In the radiographic evaluation, the articular surface and the recipient site in both groups with IGF-1 showed significantly more effective filling (P ≤ 0.05). Regardless of the group, collagen type 2 production, as assessed by immunohistochemistry, was found to be appropriate on the grafted articular surface.[...](AU)

Limb-sparing using total hip arthroplasty in a dog with femoral head osteosarcoma

Background: Osteosarcoma (OSA) is the most common primary bone tumor of the appendicular skeleton of dogs. Itmainly affects the metaphyseal region of long bones in large and giant breed dogs.The markedly aggressive and metastaticcharacter of the disease leads to an invariably poor to unfavorable prognosis.Although amputation is commonly performed,different surgical techniques may be used to preserve the limb. The most common methods of limb preserving surgeriesinvolve the use of endoprosthesis and allogeneic or autologous grafts. This report describes the successful use of total hipreplacement to treat a 3-year-old male dog, with OSA in the femoral head and neck.Case: This report describes the successful use of total hip replacement to treat a 3-year-old male dog, with OSA in thefemoral head and neck. The OSA stage IA located in the femoral head and neck was resected and treated through thelimb-sparing. The bone defect and joint function was reconstructed with total hip arthroplasty technique using a cementlesship prosthesis. The result of the histopathological analysis of the excised bone tissue showed a minimally productiveosteoblastic osteosarcoma. To date, the patient shows satisfactory movement rate and motion range, with no pain to palpationand without lameness in that limb. The radiographic follow-up after 24 months showed no local recurrence, metastasispulmonary or complications related to the implant. Total hip arthroplasty resulted in safe recovery of orthopedic signsassociated with osteosarcoma of the femoral head and neck [...]

Limb-sparing using total hip arthroplasty in a dog with femoral head osteosarcoma

Background: Osteosarcoma (OSA) is the most common primary bone tumor of the appendicular skeleton of dogs. Itmainly affects the metaphyseal region of long bones in large and giant breed dogs.The markedly aggressive and metastaticcharacter of the disease leads to an invariably poor to unfavorable prognosis.Although amputation is commonly performed,different surgical techniques may be used to preserve the limb. The most common methods of limb preserving surgeriesinvolve the use of endoprosthesis and allogeneic or autologous grafts. This report describes the successful use of total hipreplacement to treat a 3-year-old male dog, with OSA in the femoral head and neck.Case: This report describes the successful use of total hip replacement to treat a 3-year-old male dog, with OSA in thefemoral head and neck. The OSA stage IA located in the femoral head and neck was resected and treated through thelimb-sparing. The bone defect and joint function was reconstructed with total hip arthroplasty technique using a cementlesship prosthesis. The result of the histopathological analysis of the excised bone tissue showed a minimally productiveosteoblastic osteosarcoma. To date, the patient shows satisfactory movement rate and motion range, with no pain to palpationand without lameness in that limb. The radiographic follow-up after 24 months showed no local recurrence, metastasispulmonary or complications related to the implant. Total hip arthroplasty resulted in safe recovery of orthopedic signsassociated with osteosarcoma of the femoral head and neck [...](AU)

Femoral fracture repair in a ferret (Mustela putorius furo) / Tratamento de fratura femoral em ferret (Mustela putorius furo)

Background: Ferrets (Mustela putorius furo) are increasingly popular as pets. They require similar medical and surgical care as small animals, and orthopedic lesions are more and more common. Fracture diagnosis is based on history, orthopedic exam, and diagnostic imaging. The preferred treatment for femur fractures is surgery, which has a favorable outcome in the majority of cases. However, surgical fracture repair in ferrets can be challenging due to the small size of the bones. The objective of this paper is to report the surgical repair of a femoral fracture in a ferret using an external skeletal fixation system type IA connected to an intramedullary pin (tie-in configuration). Case: An adult ferret was referred to the veterinary hospital after being stepped on. The ferret was diagnosed with an oblique diaphyseal fracture of the left femur and surgery was recommended. Open fracture reduction was performed with a 1 mm intramedullary pin (Kirschner wire) placed in a retrograde direction into the proximal bone segment. After fracture reduction, the intramedullary pin was inserted into the distal bone segment. The external skeletal fixator type IA was created by the insertion of a 1.5 mm Schanz pin into the distal diaphysis and another, also 1.5 mm, inserted into the proximal metaphysis, both percutaneously. The three pins were connected externally to an aluminum bar with the [...]

Femoral fracture repair in a ferret (Mustela putorius furo) / Tratamento de fratura femoral em ferret (Mustela putorius furo)

Background: Ferrets (Mustela putorius furo) are increasingly popular as pets. They require similar medical and surgical care as small animals, and orthopedic lesions are more and more common. Fracture diagnosis is based on history, orthopedic exam, and diagnostic imaging. The preferred treatment for femur fractures is surgery, which has a favorable outcome in the majority of cases. However, surgical fracture repair in ferrets can be challenging due to the small size of the bones. The objective of this paper is to report the surgical repair of a femoral fracture in a ferret using an external skeletal fixation system type IA connected to an intramedullary pin (tie-in configuration). Case: An adult ferret was referred to the veterinary hospital after being stepped on. The ferret was diagnosed with an oblique diaphyseal fracture of the left femur and surgery was recommended. Open fracture reduction was performed with a 1 mm intramedullary pin (Kirschner wire) placed in a retrograde direction into the proximal bone segment. After fracture reduction, the intramedullary pin was inserted into the distal bone segment. The external skeletal fixator type IA was created by the insertion of a 1.5 mm Schanz pin into the distal diaphysis and another, also 1.5 mm, inserted into the proximal metaphysis, both percutaneously. The three pins were connected externally to an aluminum bar with the [...](AU)