Percutaneous endoscopic retrieval of gizzard foreign body in a goose using an operative telescope

Background: Foreign bodies are most commonly diagnosed in stomach (proventriculus and gizzard) of galliformes birds and waterfowl. Endoscopyis routinely used to assess birds respiratory, gastrointestinal and urogenital tracts. Endosurgeryis also used for sex determination and intra-coelomaticorgan biopsy. Benefits of endoscopic approaches are widely reported in birds. Conventional surgical approaches are far more invasive and risky for those patients. Thus, the purpose of this study was to describe a successful case of gizzard foreign body removal in a greylag goose (Anseranser), using a rigid endoscopic approach through a minimally invasive percutaneous access.Case: A 2-year-old female greylag goose, weighting 3,116 g, was referred to a Veterinary Teaching Hospital presenting apathy and anorexia for at least 24 h. The results on hematologic assessment were within normal range for the species. Radiographic examination indicated presence of radiopaque content, resembling gizzard sediment. Thus, the patient undergone to endoscopy for examination and aspiration of the content. The goose was fasted for 6 h. Anesthesia was induced by face mask and maintained by endotracheal tube, using isoflurane vaporized in 100% oxygen. The patient was positioned in the lateral recumbence. The patients neck was longer than the working length of the rigid endoscope. Thus, the telescope was [...]

Percutaneous endoscopic retrieval of gizzard foreign body in a goose using an operative telescope

Background: Foreign bodies are most commonly diagnosed in stomach (proventriculus and gizzard) of galliformes birds and waterfowl. Endoscopyis routinely used to assess birds respiratory, gastrointestinal and urogenital tracts. Endosurgeryis also used for sex determination and intra-coelomaticorgan biopsy. Benefits of endoscopic approaches are widely reported in birds. Conventional surgical approaches are far more invasive and risky for those patients. Thus, the purpose of this study was to describe a successful case of gizzard foreign body removal in a greylag goose (Anseranser), using a rigid endoscopic approach through a minimally invasive percutaneous access.Case: A 2-year-old female greylag goose, weighting 3,116 g, was referred to a Veterinary Teaching Hospital presenting apathy and anorexia for at least 24 h. The results on hematologic assessment were within normal range for the species. Radiographic examination indicated presence of radiopaque content, resembling gizzard sediment. Thus, the patient undergone to endoscopy for examination and aspiration of the content. The goose was fasted for 6 h. Anesthesia was induced by face mask and maintained by endotracheal tube, using isoflurane vaporized in 100% oxygen. The patient was positioned in the lateral recumbence. The patients neck was longer than the working length of the rigid endoscope. Thus, the telescope was [...](AU)

Retinal dysplasia in the crab-eating fox (Cerdocyon thous) / Displasia retiniana em cachorro-do-mato (Cerdocyon thous)

Previously described in humans and domestic animals, retinal dysplasia has three clinical forms: focal/multifocal, geographic and total. A young orphan crab-eating fox (Cerdocyon thous) from wildlife, male, approximately 45 days old referred to the Wildlife Medicine and Ophthalmology Services of the "Governador Laudo Natel" Veterinary Hospital of the Universidade Estadual Paulista, Jaboticabal Campus, SP, Brazil, where it received primary outpatient care. The patient was in good general health condition, without hematological, biochemistry or serological alterations and no signs of visual impairment. Indirect binocular ophthalmoscopy showed retinal changes in the left eye, distributed over the tapetal area in the form of grayish folds and rosettes. In the affected areas, tapetal reflectivity was reduced. No other ophthalmic abnormalities were observed. This is the first report of retinal dysplasia in the crab-eating fox (Cerdocyon thous) from wildlife.(AU)

Descrita no homem e em animais domésticos, a displasia de retina, se apresenta nas formas focal/multifocal, geográfica e completa. Um espécime de cachorro-do-mato (Cerdocyon thous) de vida livre, macho, com 45 dias de vida foi capturado e encaminhado aos Serviços de Medicina de Animais Selvagens e de Oftalmologia do Hospital Veterinário "Governador Laudo Natel" da Universidade Estadual Paulista ­ Unesp, Câmpus Jaboticabal-SP, Brasil, onde recebeu atendimento primário ambulatorial. O paciente apresentava-se em bom estado geral, sem alterações hematológicas e sorológicas, e não havia sinais de déficit visual. A oftalmoscopia binocular indireta mostrou alterações retinianas no olho esquerdo, distribuídas na área tapetal na forma de pregas e de rosetas de coloração acinzentada. Nas áreas acometidas, a reflectividade tapetal estava reduzida. Não foram observadas outras alterações oftálmicas. Trata-se do primeiro relato de literatura sobre displasia retiniana em cachorrodo-mato (Cerdocyon thous).(AU)

Retinal dysplasia in the crab-eating fox (Cerdocyon thous) / Displasia Retiniana em cachorro-do-mato (Cerdocyon thous)

Previously described in humans and domestic animals, retinal dysplasia has three clinical forms: focal/multifocal, geographic and total. A young orphan crab-eating fox (Cerdocyon thous) from wildlife, male, approximately 45 days old referred to the Wildlife Medicine and Ophthalmology Services of the Governador Laudo Natel Veterinary Hospital of the Universidade Estadual Paulista, Jaboticabal Campus, SP, Brazil, where it received primary outpatient care. The patient was in good general health condition, without hematological, biochemistry or serological alterations and no signs of visual impairment. Indirect binocular ophthalmoscopy showed retinal changes in the left eye, distributed over the tapetal area in the form of grayish folds and rosettes. In the affected areas, tapetal reflectivity was reduced. No other ophthalmic abnormalities were observed. This is the first report of retinal dysplasia in the crab-eating fox (Cerdocyon thous) from wildlife.(AU)

Descrita no homem e em animais domésticos, a displasia de retina, se apresenta nas formas focal/multifocal, geográfica e completa. Um espécime de cachorro-do-mato (Cerdocyon thous) de vida livre, macho, com 45 dias de vida foi capturado e encaminhado aos Serviços de Medicina de Animais Selvagens e de Oftalmologia do Hospital Veterinário Governador Laudo Natel da Universidade Estadual Paulista Unesp, Câmpus Jaboticabal-SP, Brasil, onde recebeu atendimento primário ambulatorial. O paciente apresentava-se em bom estado geral, sem alterações hematológicas e sorológicas, e não havia sinais de déficit visual. A oftalmoscopia binocular indireta mostrou alterações retinianas no olho esquerdo, distribuídas na área tapetal na forma de pregas e de rosetas de coloração acinzentada. Nas áreas acometidas, a reflectividade tapetal estava reduzida. Não foram observadas outras alterações oftálmicas. Trata-se do primeiro relato de literatura sobre displasia retiniana em cachorro-do-mato (Cerdocyon thous).(AU)

Vasectomy in spotted paca (Cuniculus paca)

Background: Cuniculus paca is the second largest neotropical rodent. It is not endangered, but your habitat has been destroyed and the specie has been hunted, because of its prized meat. In this context captive breeding is an alternative to reduce the hunt. Then, adult male vasectomy is an interesting alternative for Cuniculus paca since the animal does not lose libido and maintain cyclicity of females into the enclosure. This technique is a method of sterilization which the vas deferens is surgically clamped, cut, or otherwise sealed and thus prevents the release of sperm when a male ejaculates. The aim of this study was to describe the vasectomy technique on a male spotted paca kept in captive. Case: A captive adult male of Cuniculus paca, lived in Brazilian wild fauna breeding for scientific research. It was maintained on precinct with no other animal, ate fruits, vegetables, tubers and rodent chow and water offered ad libitum. It was submitted to bilateral vasectomy to maintain reproductive behavior on bevy, but not impregnate females. The anaesthesia was performed using ketamine hydrochloride (25 mg/kg IM) and midazolam (0.5 mg/kg IM) as premedication, and isoflurane in open system by facemask diluted in 100% O2 for induction and maintenance. Immediately after induction, was performed epidural anesthesia using 4 mg/kg of lidocaine hydrochloride without vasoconstrictor [...]

Vasectomy in spotted paca (Cuniculus paca)

Background: Cuniculus paca is the second largest neotropical rodent. It is not endangered, but your habitat has been destroyed and the specie has been hunted, because of its prized meat. In this context captive breeding is an alternative to reduce the hunt. Then, adult male vasectomy is an interesting alternative for Cuniculus paca since the animal does not lose libido and maintain cyclicity of females into the enclosure. This technique is a method of sterilization which the vas deferens is surgically clamped, cut, or otherwise sealed and thus prevents the release of sperm when a male ejaculates. The aim of this study was to describe the vasectomy technique on a male spotted paca kept in captive. Case: A captive adult male of Cuniculus paca, lived in Brazilian wild fauna breeding for scientific research. It was maintained on precinct with no other animal, ate fruits, vegetables, tubers and rodent chow and water offered ad libitum. It was submitted to bilateral vasectomy to maintain reproductive behavior on bevy, but not impregnate females. The anaesthesia was performed using ketamine hydrochloride (25 mg/kg IM) and midazolam (0.5 mg/kg IM) as premedication, and isoflurane in open system by facemask diluted in 100% O2 for induction and maintenance. Immediately after induction, was performed epidural anesthesia using 4 mg/kg of lidocaine hydrochloride without vasoconstrictor [...](AU)

Estudio de la madurez fetal canina al final del embarazo: aplicabilidad de diagnóstico / Study of canine fetal maturity, emphasizing final stage of gestation, and diagnostic applicability / Estudo da maturidade fetal canina em fase final de gestação: aplicabilidade diagnóstica

En la medicina, las técnicas para la evaluación de la madurez esquelética, pulmón, riñón y corazón ya se usan de forma rutinaria en obstetricia, a diferencia de veterinaria que estas evaluaciones están siendo estudiados recientemente. La evaluación de los fluidos fetales se convierte en obstetricia humana para un parámetro para la determinación de la madurez fetal, embarazo de alto riesgo, muerte fetal, infección fetal y el sexo. Mientras que para los pequeños animales es necesario desarrollar técnicas aplicables para determinar la maduración fetal ción de los fetos, el presente trabajo tiene como objetivo revisar o describir la aplicación de estos métodos y la evaluación de la madurez fetal en animales pequeños.(AU)

In medicine, techniques for assessing skeletal maturity, lung, kidney and heart are already routinely used in obstetrics, unlike veterinary that these assessments are being studied recently. The assessment of fetal fluids becomes for obstetrics human a parameter for determining fetal maturity, high-risk pregnancy, fetal death, fetal infection and sex. Whereas for small animals it is necessary to develop techniques applicable to determine the fetal maturation of fetuses, this paper aims to review or describe the application of these methods and assessment of fetal maturity in small animals.(AU)

Em medicina, técnicas para avaliar maturidade óssea, pulmonar, renal e cardíaca são rotineiramente utilizadas em obstetrícia, diferentemente da veterinária em que essas avaliações estão sendo recentemente estudadas. A avaliação dos fluidos fetais torna-se para a obstetrícia humana um parâmetro de determinação da maturidade fetal, gestação de alto risco, morte fetal, infecção e sexo fetais. Considerando que para pequenos animais faz-se necessário o desenvolvimento de técnicas para determinação da maturação fetal dos conceptos, a presente revisão tem como objetivo descrever a aplicação de métodos diagnósticos para avaliação da maturidade fetal em pequenos animais.(AU)

Single-port laparoscopic ovariectomy using a pre-tied loop ligature in Santa Ines ewes / Ovariectomia por um portal laparoscópico com aplicação de ligaduras pré-montadas em ovelhas Santa Inês

The aim of the study was to develop and assess the feasibility, postoperative pain and inflammatory response of the single-port laparoscopic ovariectomy in ewes, using a simple pre-tied loop ligature technique. Pre-tied Meltzer's knot was employed for prophylactic hemostasis of the ovarian pedicle. Slipknot was inserted within the abdominal cavity through a 14-gauge needle and tied surrounding the ovarian pedicle. Mean surgical time, manipulation, ligature and resection of each ovary and anesthesia time were 63±20, 20±10 and 91±26 minutes, respectively. No bleeding occurred during the surgeries. Ewes showed low scores pain (0.5±0.5) at all time-points. Postsurgical plasma fibrinogen was within the normal range for sheep specie at all time-points. The ewes showed a significant weight gain in comparison to the basal scaling (one day before the surgery). Single-port laparoscopic ovariectomy using a pre-tied loop ligature is feasible in the ovine specie and provided minimal postoperative distress and quick weight gain.(AU)

Objetivou-se com este trabalho desenvolver e descrever uma técnica de ovariectomia por videolaparoscopia utilizando um portal laparoscópico e um sistema de ligadura pré-montada, avaliando a sua viabilidade, o desconforto doloroso e o processo inflamatório provocado em ovelhas. O nó de Meltzer pré-montado foi utilizado para hemostasia profilática do pedículo ovariano. O nó corrediço foi inserido na cavidade abdominal através de uma agulha 14G e atado em torno do pedículo ovariano. O tempo médio de cirurgia foi de 63±20min, o de manipulação, ligadura e ressecção para cada ovário foi de 20±10min, e o de anestesia 91±26min. Não houve hemorragia durante as cirurgias. As ovelhas apresentaram escores de dor considerados baixos (0,5±0,5). Todos os valores do fibrinogênio plasmático estiveram dentro do padrão de normalidade, não havendo diferença estatística entre os momentos avaliados. Houve aumento significativo nas médias de peso das fêmeas, quando comparados ao momento controle (um dia anterior ao experimento). A ovariectomia por um portal laparoscópico com aplicação de ligaduras pré-montadas é factível para a espécie ovina, provocando mínimo estresse, desconforto doloroso e rápido ganho de peso nos animais.(AU)

Conventional and doppler abdominal ultrasonography in pacas (Cuniculus paca)

Background: The first report of ultrasound evaluation in pacas was for gestational diagnosis and monitoring. However,there are no reports of the use of this technique for the evaluation of abdominal structure in pacas. Elucidating the biologyof wild species is important for preservation and potential use as experimental models for human and animal research. Assuch, this study aimed to describe an ultrasonographic view of major abdominal organs and determine the vascular indicesof abdominal aorta and renal arteries in pacas (Cuniculus paca) by Doppler mode.Materials, Methods & Results: Fifteen adult females with a mean weight of nine kilograms were positioned into dorsal orlateral recumbency. A specific gel was applied, and transabdominal ultrasound examination was performed by a single experienced evaluator using MyLab 30 VET ultrasound equipment (ESAOTE, Italy) with 7.5 and 10.0 MHz linear transducers.Anatomy, biometry and ultrasonographic findings, such as ecogenicity, echotexture and wall thickness of main abdominalorgans (bladder, kidneys, adrenals, spleen, liver, digestive system and ovaries), were assessed during the ultrasound scan.Doppler ultrasound was used to determine the peak systolic velocity (PSV), end-diastolic velocity (EDV), resistance index (RI = [PSV - EDV]/PSV) and pulsatility index (PI = [PSV - EDV]/mean velocity) of the abdominal aorta and renalarteries. The animals were healthy based on physical examination, hemogram and biochemical profile. Furthermore, wedetermined that the ultrasound technique for abdominal evaluation of these animals was feasible. Major abdominal organswere accessed using conventional ultrasonography biometric values and sonographic characteristics. Doppler examinationwas used to determine mean vascular indices of the abdominal aorta and the left and right renal arteries...(AU)

Conventional and doppler abdominal ultrasonography in pacas (Cuniculus paca)

Background: The first report of ultrasound evaluation in pacas was for gestational diagnosis and monitoring. However,there are no reports of the use of this technique for the evaluation of abdominal structure in pacas. Elucidating the biologyof wild species is important for preservation and potential use as experimental models for human and animal research. Assuch, this study aimed to describe an ultrasonographic view of major abdominal organs and determine the vascular indicesof abdominal aorta and renal arteries in pacas (Cuniculus paca) by Doppler mode.Materials, Methods & Results: Fifteen adult females with a mean weight of nine kilograms were positioned into dorsal orlateral recumbency. A specific gel was applied, and transabdominal ultrasound examination was performed by a single experienced evaluator using MyLab 30 VET ultrasound equipment (ESAOTE, Italy) with 7.5 and 10.0 MHz linear transducers.Anatomy, biometry and ultrasonographic findings, such as ecogenicity, echotexture and wall thickness of main abdominalorgans (bladder, kidneys, adrenals, spleen, liver, digestive system and ovaries), were assessed during the ultrasound scan.Doppler ultrasound was used to determine the peak systolic velocity (PSV), end-diastolic velocity (EDV), resistance index (RI = [PSV - EDV]/PSV) and pulsatility index (PI = [PSV - EDV]/mean velocity) of the abdominal aorta and renalarteries. The animals were healthy based on physical examination, hemogram and biochemical profile. Furthermore, wedetermined that the ultrasound technique for abdominal evaluation of these animals was feasible. Major abdominal organswere accessed using conventional ultrasonography biometric values and sonographic characteristics. Doppler examinationwas used to determine mean vascular indices of the abdominal aorta and the left and right renal arteries...

Use of a standard operative laparoscope for ventriculus foreign body removal in a Toco Toucan (Ramphastos toco)

Background: Cases of ventricular foreign bodies are common in some species of birds. Moreover, ventricular impactions may develop depending on the type of the material ingested. The diagnosis is based on physical examination, radiography, endoscopy and surgical exploration. The choice of the treatment basically depends on the nature of the foreign body. Endoscopy is a less invasive approach that can be used in some situations, in some species. The benefits of endoscopic approaches are wide, as traditional approaches are more invasive and inflict a higher postoperative morbidity and mortality. However, the correct endoscope technique never been described. This study reports a case of a foreign body in a toucan's ventriculus, removed using a standard operative laparoscope/nephroscope. Case: A young Toco Toucan (Ramphastos toco) was referred to the Veterinary Hospital (São Paulo State University - FCAV/UNESP) by the State Environmental Guard. Survey radiographs indicated a foreign body in the ventriculus. The physical exam revealed apathy, but the bird was responsive to handling. The patient was pinioned. Based on the size of the foreign body, the endoscope retrieval was the first treatment option. The patient was fasted for 6 hours and anesthetized with isoflurane. The rigid endoscope/laparoscope with working channel was carefully passed through the esophagus, into the ventriculus. This view was impaired by a large amount of fibrin and gastroesophageal mucus, which was rinsed with normal saline and aspirated through the operating channel. There was marked hyperemia on the ventriculus mucosa and a large amount of fibrin and gastroesophageal mucus. The foreign body was retrieved using a standard 10-mm 0° rigid laparoscope/nephroscope with working channel, and a 42 cm long, 5-mm atraumatic laparoscopic Babcock forceps by gentile traction and axial rotation. The overall intervention time was 24 min, since mask induction until foreign body retrieve. The patient recovered with no complications in the early postoperative period and convalesced completely and uneventfully following 2 days. Discussion: The operative rigid endoscope allowed for both visualization of the oral cavity, esophagus, proventriculus and ventriculus and foreign body removal, through the working channel, demonstrating the potential benefits and feasibility of such approach in birds. Nevertheless, up to date, endoscopy have been used in toucans only for sex determination. The current report demonstrated the effectiveness of endoscopy in order to avoid the risk of death due to a surgical approach. Gastrointestinal foreign bodies are common indication for endoscopic approach. If the foreign body is present in the crop, it is easily removed with the use of an endoscope and grasper forceps, or by ingluviotomy. Even though the foreign body passes into the proventriculus or ventriculus, rigid endoscopy may still be the best treatment option. The 30º Trendelenburg positioning was crucial for the complete success of the procedure, not only for avoiding the risk of fluid aspiration, but also for improving the access to the ventriculus.

Pesquisa de agentes virais de doenças hemorrágicas em cervídeos brasileiros : Imunodetecção de células-tronco tumorais em neoplasias mamárias caninas

Os cervídeos têm sido acometidos por doenças hemorrágicas virais, tais como, doença epizoótica hemorrágica (DEH), língua azul (LA) e doença hemorrágica por adenovírus (DHA). Como as lesões macroscópicas, entre elas, enterite hemorrágica, edema pulmonar, petéquias e sufusões em diversos órgãos, são observadas nas três doenças, há necessidade de técnicas acuradas para realizar o diagnóstico definitivo. A partir do material de arquivo (blocos de parafina) existente no Departamento de Patologia Veterinária da FCAV ? Unesp, 42 cervídeos brasileiros, tanto de vida livre como de cativeiro, foram selecionados, por apresentarem sinais clínicos e/ou lesões macroscópicas sugestivas de doenças virais hemorrágicas. Das amostras analisadas, utilizando técnica de imunoistoquímica, todas apresentaram resultado negativo para adenovírus. Utilizando técnica de RT-PCR em tempo real para vírus da doença epizoótica hemorrágica, os resultados foram também negativos. A mesma técnica aplicada para vírus da língua azul revelou sete animais positivos (16,66%) confirmados com eletroforese em gel de agarose 4% e sequenciamento. Todos os casos positivos foram de animais provenientes de cativeiro, sendo três fêmeas (duas jovens, uma adulta) e quatro machos jovens. As principais alterações macroscópicas observadas nesses animais foram conteúdo intestinal hemorrágico, mucosas avermelhadas do trato gastrointestinal, úlceras em língua e petéquias em diversos órgãos. Na histologia observou-se principalmente infiltrado inflamatório, hemorragia e congestão em diversos órgãos. Os vírus da DHA e DEH não estão envolvidos nos óbitos dos cervídeos estudados. A relevância deste trabalho está no fato de ser a primeira descrição de material genético do vírus da LA em cervídeos brasileiros

Cervids have been affected by viral hemorrhagic diseases, such as Epizootic hemorrhagic disease (EHD), Bluetongue (BT), and Adenoviral hemorrhagic disease (AHD). Once that gross lesions, among them, hemorrhagic enteritis, pulmonary edema, petechiae and suffusions in several organs, are similar, it is necessary to use accurate techniques to the definitive diagnosis. From the archival material (paraffin blocks) available in the Department of Veterinary Pathology of FCAV ? Unesp, 42 Brazilian deer, both free living and captive, were selected because they had lesions suggestive of hemorrhagic viral disease. The samples analyzed, using Immunohistochemistry, were all negative for adenovirus. Using real time RT-PCR for EHD, the results were also negative. The same technique applied to BT virus revealed seven positive animals (16,66%) confirmed after agarose 4% gel electrophoresis and gene sequencing. The main macroscopic changes observed in these animals were hemorrhagic intestinal contents, reddish mucous membrane of the gastrointestinal tract, ulcers on tongue and petechiae in various organs. Mostly histological changes observed were inflammatory infiltrate, hemorrhage, and congestion in various organs. All positive cases were from captive animals, three females (two young and one adult), and four young males. The AHD and EHD virus are not involved in the deaths of the deers studied in this research. The significance of this study is due to the fact that it was the first time the genome of BT virus was identified in Brazilian cervids

Estudio de la madurez fetal canina al final del embarazo: aplicabilidad de diagnóstico / Study of canine fetal maturity, emphasizing final stage of gestation, and diagnostic applicability / Estudo da maturidade fetal canina em fase final de gestação: aplicabilidade diagnóstica

En la medicina, las técnicas para la evaluación de la madurez esquelética, pulmón, riñón y corazón ya se usan de forma rutinaria en obstetricia, a diferencia de veterinaria que estas evaluaciones están siendo estudiados recientemente. La evaluación de los fluidos fetales se convierte en obstetricia humana para un parámetro para la determinación de la madurez fetal, embarazo de alto riesgo, muerte fetal, infección fetal y el sexo. Mientras que para los pequeños animales es necesario desarrollar técnicas aplicables para determinar la maduración fetal ción de los fetos, el presente trabajo tiene como objetivo revisar o describir la aplicación de estos métodos y la evaluación de la madurez fetal en animales pequeños.

In medicine, techniques for assessing skeletal maturity, lung, kidney and heart are already routinely used in obstetrics, unlike veterinary that these assessments are being studied recently. The assessment of fetal fluids becomes for obstetrics human a parameter for determining fetal maturity, high-risk pregnancy, fetal death, fetal infection and sex. Whereas for small animals it is necessary to develop techniques applicable to determine the fetal maturation of fetuses, this paper aims to review or describe the application of these methods and assessment of fetal maturity in small animals.

Em medicina, técnicas para avaliar maturidade óssea, pulmonar, renal e cardíaca são rotineiramente utilizadas em obstetrícia, diferentemente da veterinária em que essas avaliações estão sendo recentemente estudadas. A avaliação dos fluidos fetais torna-se para a obstetrícia humana um parâmetro de determinação da maturidade fetal, gestação de alto risco, morte fetal, infecção e sexo fetais. Considerando que para pequenos animais faz-se necessário o desenvolvimento de técnicas para determinação da maturação fetal dos conceptos, a presente revisão tem como objetivo descrever a aplicação de métodos diagnósticos para avaliação da maturidade fetal em pequenos animais.