Effects of Lysophospholipid and Lipase Enzyme Supplementation to Low Metabolizable Energy Diets on Growth Performance, Intestinal Morphology and Microbial Population and Some Blood Metabolites in Broiler Chickens

This experiment aimed to evaluate the impacts of dietary lysophospholipid (LPL) and lipase enzyme complementation based on low-energy diets on growth performance, intestinal morphology, blood metabolites, immune response, and carcass traits in broiler chickens. Two hundreds broiler chicks were assigned to a completely randomized design with five treatments and four replications with ten one-day old chicks. The five treatments were: positive control (PC) without LPL supplementation and adequate in all nutrients, negative control (NC) without LPL the reduced 150 kcal/kg of metabolizable energy, NC+ 0.15% LPL (LPL15), NC+ lipase (NCL), NC+ 0.15% LPL+ lipase (NCLL). Feeding LPL improved body weight gain and feed conversion ratio (FCR). In contrast, lipase supplementation showed no significant improvement on weight gain and FCR. Supplementation of LPL and lipase did not have significant effect on immune organ, abdominal fat, and liver and thigh but decreased heart and gizzard and increased breast relative weight (p 0.05). Digestibility of dry matter did not show significant effect but crude protein and ether extract improved digestibility in LPL15 and NCLL group in contrast to NC group (p 0.05). Dietary treatment showed no significant improvement on the metabolic blood factors (p 0.05). The inclusion of LPL to negative diet (LPL15) and LPL+lipase to negative control diet raised villus height, ratio of villi height to crypt depth and increased crypt depth. Overall, LPL inclusion to diet increased weight gain and improved FCR, crude protein and fat digestibility, and improved villus height and ratio of villi height to crypt depth to NC group.(AU)

Effects of Lysophospholipid and Lipase Enzyme Supplementation to Low Metabolizable Energy Diets on Growth Performance, Intestinal Morphology and Microbial Population and Some Blood Metabolites in Broiler Chickens

This experiment aimed to evaluate the impacts of dietary lysophospholipid (LPL) and lipase enzyme complementation based on low-energy diets on growth performance, intestinal morphology, blood metabolites, immune response, and carcass traits in broiler chickens. Two hundreds broiler chicks were assigned to a completely randomized design with five treatments and four replications with ten one-day old chicks. The five treatments were: positive control (PC) without LPL supplementation and adequate in all nutrients, negative control (NC) without LPL the reduced 150 kcal/kg of metabolizable energy, NC+ 0.15% LPL (LPL15), NC+ lipase (NCL), NC+ 0.15% LPL+ lipase (NCLL). Feeding LPL improved body weight gain and feed conversion ratio (FCR). In contrast, lipase supplementation showed no significant improvement on weight gain and FCR. Supplementation of LPL and lipase did not have significant effect on immune organ, abdominal fat, and liver and thigh but decreased heart and gizzard and increased breast relative weight (p 0.05). Digestibility of dry matter did not show significant effect but crude protein and ether extract improved digestibility in LPL15 and NCLL group in contrast to NC group (p 0.05). Dietary treatment showed no significant improvement on the metabolic blood factors (p 0.05). The inclusion of LPL to negative diet (LPL15) and LPL+lipase to negative control diet raised villus height, ratio of villi height to crypt depth and increased crypt depth. Overall, LPL inclusion to diet increased weight gain and improved FCR, crude protein and fat digestibility, and improved villus height and ratio of villi height to crypt depth to NC group.

Effect of different levels of methionine, protein and tallow on the productive performance and egg quality of laying hens in the late-phase production

An experiment was conducted to determine the effects of different levels of methionine, protein and tallow on productive performance and egg quality of laying hens in the late phase of production. A completely randomized design with a 3×2×2 factorial arrangement, with three levels (0.34, 0.31, and 0.27%) of methionine (MET), two levels (12.8 and 14.7%) of protein (PRO) and two levels (1 and 3%) of tallow (TAL) with constant level of linoleic acid (1.55 ± 0.02%), was used. A number of 144 Hi-Line W-36 layers from 70 to 76 wk of age was randomly distributed into 12 treatment groups with 4 replicates of 3 hens each. Egg production and egg weight were daily recorded and feed intake and egg quality traits were recorded every 2 wk. There was a significant interaction between PRO levels and TAL for egg weight. Low levels of TAL and PRO decreased egg weight throughout the experiment. High levels of MET and TAL with concomitant reduced PRO, increased eggshell thickness, and a significant interaction between levels of MET, PRO and TAL was observed during the experiment (70 to 76 wk). Low level of protein (12.8%) significantly decreased albumen weight in the third 2-wk period. Yolk color increased when hens were fed low levels of PRO and TAL. Results of this experiment indicated that the simultaneous reduction of dietary PRO and MET in diets of Hi-Line W-36 laying hens in the late phase of production, reduced egg weight (P<0.05). Productive performance and egg quality were not affected by 12 and 20% reduction of PRO and MET, respectively. It seems that decreasing the levels of MET and PRO to lower than the recommended values can decrease egg weight without negative effects on productive performance and egg quality of laying hens in the late phase of production.(AU)

Effect of different levels of methionine, protein and tallow on the productive performance and egg quality of laying hens in the late-phase production

An experiment was conducted to determine the effects of different levels of methionine, protein and tallow on productive performance and egg quality of laying hens in the late phase of production. A completely randomized design with a 3×2×2 factorial arrangement, with three levels (0.34, 0.31, and 0.27%) of methionine (MET), two levels (12.8 and 14.7%) of protein (PRO) and two levels (1 and 3%) of tallow (TAL) with constant level of linoleic acid (1.55 ± 0.02%), was used. A number of 144 Hi-Line W-36 layers from 70 to 76 wk of age was randomly distributed into 12 treatment groups with 4 replicates of 3 hens each. Egg production and egg weight were daily recorded and feed intake and egg quality traits were recorded every 2 wk. There was a significant interaction between PRO levels and TAL for egg weight. Low levels of TAL and PRO decreased egg weight throughout the experiment. High levels of MET and TAL with concomitant reduced PRO, increased eggshell thickness, and a significant interaction between levels of MET, PRO and TAL was observed during the experiment (70 to 76 wk). Low level of protein (12.8%) significantly decreased albumen weight in the third 2-wk period. Yolk color increased when hens were fed low levels of PRO and TAL. Results of this experiment indicated that the simultaneous reduction of dietary PRO and MET in diets of Hi-Line W-36 laying hens in the late phase of production, reduced egg weight (P 0.05). Productive performance and egg quality were not affected by 12 and 20% reduction of PRO and MET, respectively. It seems that decreasing the levels of MET and PRO to lower than the recommended values can decrease egg weight without negative effects on productive performance and egg quality of laying hens in the late phase of production.