Isolation of Staphylococcus from minas frescal type cheese and detection of enterotoxin genes

This work aimed at isolating Staphylococcus spp. from minas frescal type cheese, and for this purpose a Multiplex PCR (M-PCR) was standardized for detecting the classical Staphylococcus aureus enterotoxingenes (sea, seb, sec, sed and see), using the femA gene as a positive control for S. aureus strains. Bacterial detection directly from the minas frescal type cheese and from artificially contaminated food was tested.One hundred eleven colonies (104 coagulase-positive and seven coagulase-negative) were selected for performing M-PCR assay. Thirty-four colonies (30.62%) were positive for at least one of the fiveente rotoxin genes analyzed; enterotoxins sea and seb were the most frequently detected. The study on Staphylococci coagulase-positive samples revealed that 40% of the samples showed bacterial counts above the limit established by Brazilian legislation. (AU)

Detecção de patógenos presentes nos alimentos: a falta de padronização e validação de métodos moleculares no Brasil / Detention of pathogen in foods: the lack of standardization and validation of molecular methods in Brazil

Segundo a Organização Mundial de Saúde (OMS), as doenças de origem alimentar são definidas como doenças usualmente infecciosas ou tóxicas, causadas por agentes que penetram no hospedeiro através da ingestão de alimentos, sendo que todas as pessoas estão sujeitas às doenças de origem alimentar. Entretanto, as crianças menores de 10 anos e os idosos, acima dos 60 anos, são os mais afetados pelos patógenos, assim como as pessoas imunodeprimidas. Com este artigo, espera-se incentivar não só uma maior utilização da técnica de PCR para a detecção de microrganismos patogênicos ao homem presentes nos alimentos, mais também prosseguir com padronização e validação do método e complementar a RDC 12 com a detecção molecular de outros microrganismos de grande importância para a saúde pública.(AU)

According to World Health Organization (WHO) foodborne illnesses are defined as diseases, usually either infectiousor toxic in nature, caused by agents that enter the body through the ingestion of food and all people are subject offoodborne illness. However, children under 10 years of age and the ederly, above of the 60 years, are the most affected by the pathogens, as well as the immunocompromised people. With this paper, be expect to stimulate a bigger use of the PCR to the molecular detection of pathogenic microorganims and to continue with the standardization and validation of the method and to complement RDC 12 with the molecular detection of other microorganisms of great importance for the public health. (AU)