Resumo
No primeiro experimento foram utilizados oócitos de ovários de abatedouro distribuídos em seis grupos: 1) Maturação e Cultivo in vitro sem adição de antioxidantes (Ct-Ct); 2) Maturação com 2 M de quercetina (Qc-Ct); 3) Cultivo com 2 M de quercetina (Ct-Qc); 4) Maturação e Cultivo com 2 M de quercetina (Qc-Qc); 5) Maturação com 100 M de cisteamina e Cultivo com de 2 M de quercetina (Cis-Qc) e 6) Maturação com cisteamina (Cis-Ct). Com base no primeiro experimento, os oócitos provenientes de Ovum pick up foram avaliados nos grupos Cis-Qc e Cis-Ct. Os resultados do experimento 1 e 2 para os grupos Cis-Qc e Cis-Ct foram confrontados. A análise estatística considerou significância de 5% nas avaliações. O percentual médio de desenvolvimento dos embriões foi similar entre os grupos Qc-Ct, Ct-Qc, Cis-Qc e Cis-Ct (49,55±5.1), porém superiores aos grupos Ct-Ct e Qc-Qc (42,4±6.1). A taxa média de blastocistos eclodidos foi similar entre os grupos Ct-Ct, Qc-Ct, CisQc e Cis-Ct (60,9±6.3), e estes superior grupo Qc-Qc (54,7±9.7). Para o número médio de células dos blastocistos o grupo Cis-Ct (145±25) foi superior os grupos Cis-Qc, Ct-Ct e Qc-Ct (128±25) e estes superiores os grupos Ct-Qc e Qc-Qc (109±32). No experimento 2 a taxa de clivagem não diferiu entre Cis-Ct (88,8±3.9) e Cis-Qc (87,7±4.5). Mas o percentual de blastocistos foi superior para Cis-Qc (64,6±9.8) em relação a Cis-Ct (56,4±3.6). Enquanto a taxa de prenhez do grupo 2 Cis-Ct (45,1±2.5) foi superior ao Cis-Qc (38,5±3.5). O percentual de blastocisto do experimento 2 (56,4±3.6 e 64,6±9.8%) foram superiores aos do experimento 1 (48,9±3.6 e 52,2±5.1%) para os grupos Cis-Ct e Cis-Qc, respectivamente. Em conclusão, a produção in vitro de embriões suplementada com antioxidante dependendo do grupo foi positiva, mas este benefício não foi confirmado na taxa de prenhez com o uso da quercetina no cultivo in vitro
In the first experiment we used oocytes from ovaries of slaughterhouse distributed in six groups: 1) Maturation and cultivation without the addition of antioxidants (Ct-Ct); 2) Maturation with 2 M of quercetin (Qc-Ct); 3) Cultivation with 2 M of quercetin (Ct-Qc); 4) Maturation and cultivation with 2 M of quercetin (Qc-Qc); 5) Maturation with 100 M of cysteamine and cultivation with of 2 M of quercetin (CisQc) and 6) Maturation with cysteamine (Cis-Ct). Based in experiment 1, the oocytes of Ovum pick up (OPU) were evaluated in Cis-and Cis-Qc CT. for experiment 3 were assessed the results of experiment 1 and 2 for groups Cis-and Cis-Ct Qc. Statistical analysis significance of 5% was considered in reviews. The percentage of embryos was superior to Qc-Ct, Ct-Qc, Cis-and Cis-Ct Qc (49,55±5.1), about Ct-Ct and QcQc (42.4±6.1). The hatching rate was similar to Ct-Ct, Qc-Ct, Cis-and Cis-Ct Qc (60,9±6.3), however the Group Qc-Qc (54,7±9.7). Had the lowest rate of hatching, followed by the Ct-Qc (57.3 ± 11.0). The number of cells in the embryos of the Group Cis-Ct (145±25) was superior to the Cis-Qc groups, Ct-Ct and Qc-Ct (media 128 ± 25). The embryos of Ct-Qc groups and Qc-Qc (media 109 ± 32) had the fewest cell. The rate of cleavage in the oocytes OPU did not differ between Cis-Ct (88.8±3.9) and Cis-Qc (87.7 ± 4.5). But the percentae of blastocyst was superior to Cis-Qc (64.6 ± 9.8) for the Cis-Ct (56.4 ± 3.6). However, the rate of pregnancy blastocyst Cis group- 4 Ct (45.1±2.5) were higher than those of Cis-Qc (38.5±3.5). The percentage of blastocyst OPU oocytes (56.4 ± 3.6 and 64.6 ± 9.8%) were higher than those of slaughterhouse (48.9 ± 3.6 and 52.2 ± 5.1%) independent of the groups evaluated (Cis-and Cis-Qc Ct), respectively. In conclusion, the in vitro production of embryos supplemented with antioxidant depending on the Group was positive, but this benefit has not been confirmed pregnant rate with the use of quercetin on in vitro cultivation.