Increasing soybean productivity by plant equidistant arrangements and modification of technology levels of cropping systems / Incrementos produtivos na cultura da soja pelo uso de arranjos equidistantes e níveis tecnológicos nos sistemas de cultivo

The arrangement of soybean plants defines their ability to recognize the environment and improve their abiotic and biotic interactions with it. This study aimed to evaluate the effect of planting arrangements associated to two distinct crop systems (high technological level or conventional crop level) for the soybean varieties 8473 RSF and AS 3730, to evaluate the plant performance and productivity in the field. A double factorial scheme was used with two varieties and six spatial arrangements. Two experiments were conducted during the 2017­2018 crop season: the first was based on standard cultivation of the Brazilian Cerrado (without irrigation systems and adequate fertilizer), whereas the second utilized high technology (irrigation systems and increased fertilizer). The morphophysiological parameters, normalized difference vegetation index (NDVI), leaf pigment content and crop yield parameters were evaluated. Our study pointed out the positive responses for both cultivated varieties and two technological levels adopted cultivated under equidistant arrangement and high density. The leaf area index, leaf pigment content, NDVI, and crop production showed substantial responses under different equidistant arrangements. Therefore, this procedure requires adjustments in the level of cultivation technology and identification of the most suitable soybean variety.

O arranjo das plantas de soja define sua habilidade em reconhecer o ambiente e aprimorar suas interações bióticas e abióticas com este. Este estudo objetivou avaliar o efeito de diferentes arranjos de plantio associados com dois distintos sistemas de cultivo (alto nível tecnológico ou nível tecnológico convencional), nas variedades 8473 RSF e AS 3730, para avaliar a performance e produtividades das plantas à campo. O esquema fatorial duplo foi adotado, sendo: duas variedades e seis arranjos espaciais. Dois experimentos foram conduzidos durante o ano agrícola 2017-2018; no primeiro experimento considerou-se o padrão de cultivo da soja no Cerrado Brasileiro (sem sistema de irrigação a nível adequado de adubação), enquanto no segundo experimento foi adotado alto nível tecnológico (com sistema de irrigação e adubação superior aos padrões convencionados para o Cerrado). Foram avaliados parâmetros morfofisiológicos, índice de vegetação por diferença normalizada (IVDN), conteúdo de pigmentos foliares e parâmetros de produção da cultura. Nosso estudo apontou respostas positivas de ambos genótipos cultivados e sob os dois níveis tecnológicos sob os arranjos equidistantes e submetidos a alta densidade de plantio. Significativas respostas foram observadas para índice de área foliar, conteúdo de pigmentos foliares, IVDN e produção de soja, quando submetida aos diferentes arranjos equidistantes. Entretanto, este procedimento requer ajustes dentro dos níveis tecnológicos e identificação das variedades mais adequada a este modelo de cultivo.

Attenuation of salt stress by lycopene on common bean seeds

Salinity is a major abiotic stress that adversely affects several physiological and biochemical aspects of plants. Antioxidants can be used as a potential remediation mechanism to promote plant resilience. Therefore, the objective of the present study was to evaluate increasing doses of the antioxidant lycopene on the germination and initial growth of common bean seedlings under conditions of salt stress. Seeds were treated with the following lycopene concentrations: 0.018; 0.036; 0.072; 0.144; and 0.288 g L-1 and two controls (negative and positive) and evaluated under two growth conditions (without and with salt stress) for physiological quality and pigment content. Data were analyzed by completely randomized design, in 2 x 7 factorial (two growth conditions, with paper towel moistened with water or salt solution x seven treatments, including five lycopene doses and two controls), in four replicates of 50 seeds by concentration. With the results of the analysis of variance, growth conditions (without and with salt stress) were compared by the Tukey test (5%), and the effects of lycopene doses were analyzed by polynomial regression. Lycopene promoted tolerance of bean seeds to salt stress and had a positive influence on the attenuation of salt harmful effects to the initial growth of seedlings, mainly at the doses of 0.072 g L-1 and 0.144 g L-1.(AU)

Exogenous application of salicylic acid to control coffee rust

The exogenous application of salicylic acid can induce plant resistance against pathogens. However, little is known about the potential uses of this bioregulator for controlling coffee diseases. In this study, we assessed the effect of applying salicylic acid (SA – 150 mg L-1) on the management of coffee rust (Hemileia vastatrix) in a 7-year-old coffee plantation with low crop load (651.6 kg ha-1 in 2017). For comparison, plants were sprayed with protectant fungicide (copper hydroxide – CH) and standard fungicides (SF) used by local farmers (boscalid, pyraclostrobin + epoxiconazole, and copper hydroxide). Non-treated plants were included as a negative control. Five monthly applications were performed from November 2016 to March 2017. Rust incidence and severity, defoliation, and growth of plagiotropic branches were evaluated monthly. The activity of catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and total proteins was assessed one day after the first, third, and fifth product applications. Compared to untreated plants, SA reduced the severity and incidence of rust from 36.3 to 54.7%, while CH and SF reduced disease from 31.8 to 54.6% and from 83.8 to 88%, respectively. SA reduced defoliation by 54.1%. SA increased the concentration of CAT, APX, and SOD after the first application. However, this effect was not observed after subsequent applications. Foliar application of SA reduces the severity and incidence of coffee rust and defoliation in plants with a low crop load.

Exogenous application of salicylic acid to control coffee rust

The exogenous application of salicylic acid can induce plant resistance against pathogens. However, little is known about the potential uses of this bioregulator for controlling coffee diseases. In this study, we assessed the effect of applying salicylic acid (SA 150 mg L-1) on the management of coffee rust (Hemileia vastatrix) in a 7-year-old coffee plantation with low crop load (651.6 kg ha-1 in 2017). For comparison, plants were sprayed with protectant fungicide (copper hydroxide CH) and standard fungicides (SF) used by local farmers (boscalid, pyraclostrobin + epoxiconazole, and copper hydroxide). Non-treated plants were included as a negative control. Five monthly applications were performed from November 2016 to March 2017. Rust incidence and severity, defoliation, and growth of plagiotropic branches were evaluated monthly. The activity of catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and total proteins was assessed one day after the first, third, and fifth product applications. Compared to untreated plants, SA reduced the severity and incidence of rust from 36.3 to 54.7%, while CH and SF reduced disease from 31.8 to 54.6% and from 83.8 to 88%, respectively. SA reduced defoliation by 54.1%. SA increased the concentration of CAT, APX, and SOD after the first application. However, this effect was not observed after subsequent applications. Foliar application of SA reduces the severity and incidence of coffee rust and defoliation in plants with a low crop load.(AU)

Attenuation of salt stress by lycopene on common bean seeds / Attenuation of salt stress by lycopene on common bean seeds

Salinity is a major abiotic stress that adversely affects several physiological and biochemical aspects of plants. Antioxidants can be used as a potential remediation mechanism to promote plant resilience. Therefore, the objective of the present study was to evaluate increasing doses of the antioxidant lycopene on the germination and initial growth of common bean seedlings under conditions of salt stress. Seeds were treated with the following lycopene concentrations: 0.018; 0.036; 0.072; 0.144; and 0.288 g L-1 and two controls (negative and positive) and evaluated under two growth conditions (without and with salt stress) for physiological quality and pigment content. Data were analyzed by completely randomized design, in 2 x 7 factorial (two growth conditions, with paper towel moistened with water or salt solution x seven treatments, including five lycopene doses and two controls), in four replicates of 50 seeds by concentration. With the results of the analysis of variance, growth conditions (without and with salt stress) were compared by the Tukey test (5%), and the effects of lycopene doses were analyzed by polynomial regression. Lycopene promoted tolerance of bean seeds to salt stress and had a positive influence on the attenuation of salt harmful effects to the initial growth of seedlings, mainly at the doses of 0.072 g L-1 and 0.144 g L-1.

Salinity is a major abiotic stress that adversely affects several physiological and biochemical aspects of plants. Antioxidants can be used as a potential remediation mechanism to promote plant resilience. Therefore, the objective of the present study was to evaluate increasing doses of the antioxidant lycopene on the germination and initial growth of common bean seedlings under conditions of salt stress. Seeds were treated with the following lycopene concentrations: 0.018; 0.036; 0.072; 0.144; and 0.288 g L-1 and two controls (negative and positive) and evaluated under two growth conditions (without and with salt stress) for physiological quality and pigment content. Data were analyzed by completely randomized design, in 2 x 7 factorial (two growth conditions, with paper towel moistened with water or salt solution x seven treatments, including five lycopene doses and two controls), in four replicates of 50 seeds by concentration. With the results of the analysis of variance, growth conditions (without and with salt stress) were compared by the Tukey test (5%), and the effects of lycopene doses were analyzed by polynomial regression. Lycopene promoted tolerance of bean seeds to salt stress and had a positive influence on the attenuation of salt harmful effects to the initial growth of seedlings, mainly at the doses of 0.072 g L-1 and 0.144 g L-1.

Biorreguladores em feijoeiro cultivado sob dois regimes hídricos / Plant bioregulators on common bean cultivated under two soil moisture

Restrições na disponibilidade hídrica favorecem eventuais danos à cultura do feijoeiro, e o uso de biorreguladores aprimora a sinalização vegetal e potencializa a indução de tolerância ao déficit hídrico. Este estudo objetivou caracterizar alterações fisiológicas, bioquímicas e biométricas em plantas de feijão submetidas à aplicação foliar de biorreguladores sob diferentes regimes hídricos. O ensaio consistiu na aplicação de um controle (água) e três biorreguladores (5 µM de ácido abscísico - ABA; 50 µM de metil jasmonato - MeJA e 5 µM de ABA + 50 µM de MeJA), além de apresentar dois regimes hídricos (60 e 80% da capacidade de campo), num esquema fatorial 4 x 2. Foram analisados: conteúdo de pigmentos fotossintetizantes, trocas gasosas foliares e índices biométricos das plantas de feijoeiro. Com resultado observou-se que o tratamento ABA+MeJA incrementou a concentração de clorofila b, atividades de ascorbato peroxidase (APX) e catalase (CAT), enquanto a taxa fotossintética foi estimulada pela mistura de biorreguladores aos 36 dias após o plantio (DAP), ABA e MeJA, isolados, propiciaram maior taxa fotossintética no feijoeiro aos 34 DAP, enquanto para as variáveis biométricas somente foram influenciadas pelos regimes hídricos, com relação aos mecanismos bioquímicos, constatou-se que o tratamento ABA+MeJA incrementou a atividade das enzimas antioxidantes e melhorou as respostas...(AU)

Restrictions on water available lead to possible damages in the bean crop, and the use of bioregulators can improve the plant signal and boost tolerance against drought stress. This study aimed to characterize physiological, biochemical, and biometric alterations in bean plants subjected to foliar application of bioregulators under different soil water levels. The following treatments were applied: control (water) and three bioregulators (5 M of abscisic acid - ABA, 50 M of methyl jasmonate MeJA, and 5 M of ABA + 50 M of MeJA). Also, the plants were subjected to two soil water levels (60 and 80% of field capacity), in a 4 x 2 factorial scheme. The variables studied were: content of photosynthetic pigments, leaf gas exchange, and biometric indexes in the common bean plants. Our results showed that the ABA + MeJA treatment increased the concentration of chlorophyll b, ascorbate peroxidase (APX), and catalase (CAT) activities. At the same time, the photosynthetic rate was stimulated by the mixture of bioregulators on 36 days of planting (DAP). ABA and MeJA, applied isolated, caused a higher photosynthetic rate in the bean in the 34 DAP, whereas for the biometric variables, they were only influenced by the soil water levels. Regarding the biochemical mechanisms, it was verified that the ABA + MeJA treatment increased the activity of antioxidant enzymes and improved gas...(AU)

Biorreguladores em feijoeiro cultivado sob dois regimes hídricos / Plant bioregulators on common bean cultivated under two soil moisture

Restrições na disponibilidade hídrica favorecem eventuais danos à cultura do feijoeiro, e o uso de biorreguladores aprimora a sinalização vegetal e potencializa a indução de tolerância ao déficit hídrico. Este estudo objetivou caracterizar alterações fisiológicas, bioquímicas e biométricas em plantas de feijão submetidas à aplicação foliar de biorreguladores sob diferentes regimes hídricos. O ensaio consistiu na aplicação de um controle (água) e três biorreguladores (5 µM de ácido abscísico - ABA; 50 µM de metil jasmonato - MeJA e 5 µM de ABA + 50 µM de MeJA), além de apresentar dois regimes hídricos (60 e 80% da capacidade de campo), num esquema fatorial 4 x 2. Foram analisados: conteúdo de pigmentos fotossintetizantes, trocas gasosas foliares e índices biométricos das plantas de feijoeiro. Com resultado observou-se que o tratamento ABA+MeJA incrementou a concentração de clorofila b, atividades de ascorbato peroxidase (APX) e catalase (CAT), enquanto a taxa fotossintética foi estimulada pela mistura de biorreguladores aos 36 dias após o plantio (DAP), ABA e MeJA, isolados, propiciaram maior taxa fotossintética no feijoeiro aos 34 DAP, enquanto para as variáveis biométricas somente foram influenciadas pelos regimes hídricos, com relação aos mecanismos bioquímicos, constatou-se que o tratamento ABA+MeJA incrementou a atividade das enzimas antioxidantes e melhorou as respostas...

Restrictions on water available lead to possible damages in the bean crop, and the use of bioregulators can improve the plant signal and boost tolerance against drought stress. This study aimed to characterize physiological, biochemical, and biometric alterations in bean plants subjected to foliar application of bioregulators under different soil water levels. The following treatments were applied: control (water) and three bioregulators (5 M of abscisic acid - ABA, 50 M of methyl jasmonate MeJA, and 5 M of ABA + 50 M of MeJA). Also, the plants were subjected to two soil water levels (60 and 80% of field capacity), in a 4 x 2 factorial scheme. The variables studied were: content of photosynthetic pigments, leaf gas exchange, and biometric indexes in the common bean plants. Our results showed that the ABA + MeJA treatment increased the concentration of chlorophyll b, ascorbate peroxidase (APX), and catalase (CAT) activities. At the same time, the photosynthetic rate was stimulated by the mixture of bioregulators on 36 days of planting (DAP). ABA and MeJA, applied isolated, caused a higher photosynthetic rate in the bean in the 34 DAP, whereas for the biometric variables, they were only influenced by the soil water levels. Regarding the biochemical mechanisms, it was verified that the ABA + MeJA treatment increased the activity of antioxidant enzymes and improved gas...

Germinação de sementes de araticum: doses de GA3 e períodos de embebição / Araticum seeds germination: GA3 levels and soaking time

Sementes de Annona crassiflora apresentam dormência morfofisiológica, e estudos de mecanismos que permitam a sua germinação, garantirão a sobrevivência da espécie. Objetivou-se verificar ação do ácido giberélico (GA3) em distintos períodos de imersão na superação de dormência das sementes. O experimento foi conduzido em delineamento inteiramente casualizado (DIC), com 630 sementes divididas em 7 tratamentos (seccionamento mecânico + 1000 mg L-¹ de GA3 nos períodos de 24, 48 e 72 horas; seccionamento mecânico + 2000 mg L-¹ + GA3 nos períodos de 24, 48 e 72; e controle). O tratamento onde houve o seccionamento mecânico + 2000 mg L-¹ de GA3 por 72 horas aumentou a germinação das sementes em 10% quando comparado ao controle (semente somente seccionada).

The Annona crassiflora seeds showed morphophysiological dormancy and studies of breaking dormancy were necessary to the survival of the species. We evaluate de gibberellic acid (GA3) effect under different soaking times on dormancy breaking. The experimental design was completely randomized, with 630 seeds distributed in 7 treatments (mechanical scarification + 1000 mg L-¹ de GA3 in periods of 24, 48 e 72 hours; (mechanical scarification + 2000 mg L-¹ de GA3 in periods of 24, 48 e 72 hours; plus control treatment). The treatment with mechanical scarification + 2000 mg L-¹ de GA3 for 72 hours improve de seed germination in 10%, when compared to control.

Germinação de sementes de araticum: doses de GA3 e períodos de embebição / Araticum seeds germination: GA3 levels and soaking time

Sementes de Annona crassiflora apresentam dormência morfofisiológica, e estudos de mecanismos que permitam a sua germinação, garantirão a sobrevivência da espécie. Objetivou-se verificar ação do ácido giberélico (GA3) em distintos períodos de imersão na superação de dormência das sementes. O experimento foi conduzido em delineamento inteiramente casualizado (DIC), com 630 sementes divididas em 7 tratamentos (seccionamento mecânico + 1000 mg L-¹ de GA3 nos períodos de 24, 48 e 72 horas; seccionamento mecânico + 2000 mg L-¹ + GA3 nos períodos de 24, 48 e 72; e controle). O tratamento onde houve o seccionamento mecânico + 2000 mg L-¹ de GA3 por 72 horas aumentou a germinação das sementes em 10% quando comparado ao controle (semente somente seccionada).(AU)

The Annona crassiflora seeds showed morphophysiological dormancy and studies of breaking dormancy were necessary to the survival of the species. We evaluate de gibberellic acid (GA3) effect under different soaking times on dormancy breaking. The experimental design was completely randomized, with 630 seeds distributed in 7 treatments (mechanical scarification + 1000 mg L-¹ de GA3 in periods of 24, 48 e 72 hours; (mechanical scarification + 2000 mg L-¹ de GA3 in periods of 24, 48 e 72 hours; plus control treatment). The treatment with mechanical scarification + 2000 mg L-¹ de GA3 for 72 hours improve de seed germination in 10%, when compared to control.(AU)