Stephanofilariasis in Holstein cows - Diagnostic approach

Background: Stephanofilariasis is a parasitic disease caused by the nematode Stephanofilaria spp., considered a zoonotic disease, that affects several species, mainly dairy cattle. The condition causes chronic ulcerative dermatitis, due to the mechanical action of the parasite on the hair follicles and dermal papillae. The recommended diagnostic method is histopathological examination, but alternative methods have been studied for the detection of the agent since it does not demonstrate good results. There is no specific treatment, however, the use of organophosphates is recommended. The objective of this work is to report an outbreak of stephanofilariasis with unusual lesions in cattle from a dairy cattle farm in the city of Boa Vista do Buricá, RS, Brazil, as well as to demonstrate an alternative method for the diagnosis of the disease. Cases: We prospectively evaluated 15 Holstein cows, aged between 1 and 10-year-old whose data and materials for diagnosis, were provided by the property owner. The outbreak occurred in the summer, in a period of greater rainfall, affecting cows of different ages and totaling 48% of the herd. These showed ulcerative lesions on the skin of the groin, limbs and interdigital regions, and less frequently in the udder. The samples were obtained through tissue biopsy and scraped from the lesions, being packed in 10% formaldehyde for fixation. Afterward, the formaldehyde was centrifuged at 800 g for 5 min, which consisted of analyzing the sediment through optical microscopy and without staining, aiming at the direct search of the agent. The tissues, on the other hand, underwent routine processing and stained with Hematoxylin and Eosin. Histopathological analysis did not reveal Stephanofilaria sp. subjects in morphological analysis and revealed acanthosis, spongiosis, ulcers and fibrosis in the analyzed tissue. The examination of direct research with formaldehyde proved to be efficient, and fast. In the direct examination of the agent, structures similar to filarial and compatible with Stephanofilaria sp. in all samples. Thus, the present study demonstrated that filariasis can present in the form of outbreaks and with unusual injuries. The examination of direct research with formaldehyde, proved to be efficient and fast. Discussion: Semi-confined and confined animals may have a higher occurrence of strephanofilariasis, since the parasite needs humid and warm environments to proliferate and that, the proliferation of stephanophilariasis vectors is intensified in environments with low sunlight, high temperatures, high humidity, and low cleaning frequency. The city of Boa Vista do Buricá, where the outbreak occurred, reached a minimum average temperature of 18.5ºC and an average of 29ºC, with an average rainfall of 120.5 mm. Cutaneous lesions have been seen in animals from 1 to 10 years of age and no racial predisposition, age, or blackout stage is reported. The histological diagnosis, although mentioned as a form of diagnosis, has low efficacy in finding parasites in the tissue, is also used as a diagnosis, direct examination of the agent with saline solution, and impression of the lesion stained by the Romanoswsky method. However, in this report, formaldehyde proved to be a new option, as efficiently as saline.

Hipocalcemia em vacas leiteiras da agricultura familiar / Hypocalcemia in dairy cows of family agriculture

O objetivo deste trabalho foi avaliar a ocorrência de hipocalcemia associada ou não a hipomagnesemia em vacas leiteiras de propriedades de agricultura familiar. Foram avaliadas 22 vacas das raças Jersey, Holandesa e Jersolando, no período de transição, oriundas de pequenas propriedades de agricultura familiar, com produção média 12L/leite/dia. A concentração do cálcio total e do magnésio séricos foi determinada por metodologia colorimétrica e cálculo do cálcio ionizado estimado a partir das concentrações séricas do cálcio total, de proteína e albumina. Foi observada hipocalcemia em 50% dos animais no pré-parto (8,2±1,4mg/dL) e 63,6% no pós-parto (7,7±1,7 mg/dL). A redução do cálcio ionizado foi menos frequente, com grande parte dos animais apresentando cálcio ionizado estimado dentro da normalidade, com média de 4,6±0,83 mg/dL no pré-parto e 4,3±1,0 mg/dL no pós-parto. Todos os animais apresentaram concentrações de magnésio sérico dentro da normalidade, descartando-se hipomagnesemia. A concentração média de magnésio no pré-parto foi 2,4±0,3 mg/dL com elevação significativa (p<0,05) no pós-parto para 2,7±0,5 mg/dL. Conclui-se que as vacas leiteiras da agricultura familiar são susceptíveis à ocorrência de hipocalcemia e a ausência de sinais clínicos pode estar relacionada à manutenção da concentração de cálcio ionizado próxima da normalidade associada à ausência de hipomagnesemia.

The objective of this study was to evaluate the occurrence of hypocalcemia associated or not to hypomagnesemia in dairy cows of family farming properties. Were evaluated 22 cows, Jersey, Dutch and Jersolando breeds, during the transition period, from small family farming properties, with average 12 L/milk/day production. The concentration of serum total calcium and magnesium was determined by colorimetric methodology and calculation of ionized calcium estimated from the serum concentrations of total calcium, protein and albumin. Hipocalcemia was observed in 50% of the animals in prepartum (8.2 ± 1, 4 mg/dL) and 63.6% postpartum (7.7 ± 1.7 mg/dL). The reduction of ionized calcium was less frequent, with many of the animals presenting estimated ionized calcium within the normal range, averaging 4.6 ± 0.83 mg/dL in the prepartum and 4.3 ± 1.0 mg/dL in the postpartum. All animals presented concentrations of serum magnesium within normality, excluding hypomagnesemia. The average concentration of magnesium in the partum was 2.4 ± 0.3 mg/dL with significant elevation (p < 0.05) in postpartum to 2.7 ± 0.5 mg/dL. We concluded that dairy cows in the family agriculture are susceptible to hypocalcemia, and the absence of clinical signs may be relacionated with the maintenance of ionized calcium concentration into normal range, associeted with absence of hypomagnesemia.

Hipocalcemia em vacas leiteiras da agricultura familiar / Hypocalcemia in dairy cows of family agriculture

O objetivo deste trabalho foi avaliar a ocorrência de hipocalcemia associada ou não a hipomagnesemia em vacas leiteiras de propriedades de agricultura familiar. Foram avaliadas 22 vacas das raças Jersey, Holandesa e Jersolando, no período de transição, oriundas de pequenas propriedades de agricultura familiar, com produção média 12L/leite/dia. A concentração do cálcio total e do magnésio séricos foi determinada por metodologia colorimétrica e cálculo do cálcio ionizado estimado a partir das concentrações séricas do cálcio total, de proteína e albumina. Foi observada hipocalcemia em 50% dos animais no pré-parto (8,2±1,4mg/dL) e 63,6% no pós-parto (7,7±1,7 mg/dL). A redução do cálcio ionizado foi menos frequente, com grande parte dos animais apresentando cálcio ionizado estimado dentro da normalidade, com média de 4,6±0,83 mg/dL no pré-parto e 4,3±1,0 mg/dL no pós-parto. Todos os animais apresentaram concentrações de magnésio sérico dentro da normalidade, descartando-se hipomagnesemia. A concentração média de magnésio no pré-parto foi 2,4±0,3 mg/dL com elevação significativa (p<0,05) no pós-parto para 2,7±0,5 mg/dL. Conclui-se que as vacas leiteiras da agricultura familiar são susceptíveis à ocorrência de hipocalcemia e a ausência de sinais clínicos pode estar relacionada à manutenção da concentração de cálcio ionizado próxima da normalidade associada à ausência de hipomagnesemia.(AU)

The objective of this study was to evaluate the occurrence of hypocalcemia associated or not to hypomagnesemia in dairy cows of family farming properties. Were evaluated 22 cows, Jersey, Dutch and Jersolando breeds, during the transition period, from small family farming properties, with average 12 L/milk/day production. The concentration of serum total calcium and magnesium was determined by colorimetric methodology and calculation of ionized calcium estimated from the serum concentrations of total calcium, protein and albumin. Hipocalcemia was observed in 50% of the animals in prepartum (8.2 ± 1, 4 mg/dL) and 63.6% postpartum (7.7 ± 1.7 mg/dL). The reduction of ionized calcium was less frequent, with many of the animals presenting estimated ionized calcium within the normal range, averaging 4.6 ± 0.83 mg/dL in the prepartum and 4.3 ± 1.0 mg/dL in the postpartum. All animals presented concentrations of serum magnesium within normality, excluding hypomagnesemia. The average concentration of magnesium in the partum was 2.4 ± 0.3 mg/dL with significant elevation (p < 0.05) in postpartum to 2.7 ± 0.5 mg/dL. We concluded that dairy cows in the family agriculture are susceptible to hypocalcemia, and the absence of clinical signs may be relacionated with the maintenance of ionized calcium concentration into normal range, associeted with absence of hypomagnesemia.(AU)

Effect of vaquejada exercise on the physiological and biochemical profiles of sporadic competitors and atletic horses

The aim of this study was to analyze the effect of a cycle of three races as a part of the vaquejada test, on the serum biochemistry of horses that compete sporadically and athletic horses. Fourteen Quarter Horses was evaluated: group 1 (G1), with seven horses from the region that competed sporadically and group 2 (G2), with seven horses that competed regulary and were transported for >200 km. The physiological and serum biochemistry parameters were evaluated before exercise (M0) and after a cycle of three races of the vaquejada test: immediately after (M1); 30 min (M2) and 120 min (M3) after. Wilcoxon test was used to assess differences between the groups and Friedman test for moment effects, with a significance level of 5%. There was increase in M1 compared with M0 for heart and respiratory rate in G2, and rectal temperature in G1. The exercise transiently changed most of the biochemical parameters, with significant diferences for uric acid in M1, albumin and lactate in M2, compared with M0, in G2. The metabolic response to exercise was different between the groups for the following variables: uric acid, total protein, albumin, unsaturated iron binding capacity, and transferrin saturation index. It was concluded that physiological and biochemical changes induced by Vaquejada are light, transiente, and within the normal range. The changes were more expressive in athletic horses that compete regularly and are transported, demonstrating the need for studies that establish specific training and management protocols for this category.

Effect of vaquejada exercise on the physiological and biochemical profiles of sporadic competitors and atletic horses

The aim of this study was to analyze the effect of a cycle of three races as a part of the vaquejada test, on the serum biochemistry of horses that compete sporadically and athletic horses. Fourteen Quarter Horses was evaluated: group 1 (G1), with seven horses from the region that competed sporadically and group 2 (G2), with seven horses that competed regulary and were transported for >200 km. The physiological and serum biochemistry parameters were evaluated before exercise (M0) and after a cycle of three races of the vaquejada test: immediately after (M1); 30 min (M2) and 120 min (M3) after. Wilcoxon test was used to assess differences between the groups and Friedman test for moment effects, with a significance level of 5%. There was increase in M1 compared with M0 for heart and respiratory rate in G2, and rectal temperature in G1. The exercise transiently changed most of the biochemical parameters, with significant diferences for uric acid in M1, albumin and lactate in M2, compared with M0, in G2. The metabolic response to exercise was different between the groups for the following variables: uric acid, total protein, albumin, unsaturated iron binding capacity, and transferrin saturation index. It was concluded that physiological and biochemical changes induced by Vaquejada are light, transiente, and within the normal range. The changes were more expressive in athletic horses that compete regularly and are transported, demonstrating the need for studies that establish specific training and management protocols for this category.(AU)

Perfil hematológico de ovelhas santa inês suplementadas a pasto no terço final de gestação e no pós-parto / Hematological profile of Santa Inês ewes supplemented on pasture at the last third of pregnancy and postpartum

The purpose of this study was to evaluate the influence of concentrate supplementation level on hematological profile of Santa Ines ewes, at the last third of pregnancy and postpartum, and of their lambs. We used eleven ewes at the last 75 days of pregnancy and the first 75 days of lactation and eight lambs born from these ewes, allotted in a completely randomized design and split-plot arrangement in time. Blood samples were collected every fourteen days. Neither the erythrocyte nor the leukocyte profile was affected by the different levels of supplementation (P>0.05) and the blood components evaluated were within the reference range for adult sheep. The animal category affected (P 0.05) erythrocytes, and the highest values ??(11.72 x 106/?l) were observed in lambs. Lambs showed lower values ??(3073.3/µL) for neutrophils in comparison with ewes, during pregnancy (4957.6/µL) or postpartum (4067.3/µL); these values did not differ (P>0.05). For the lymphocyte count, the values ??found in lambs (2858.8 /µL) were similar to those in pregnant ewes (2,982.0/µL); lactating ewes (4119.8/µL) showed the highest values. Concentrate supplementation (0.5% BW) at pre and postpartum does not alter the erythrocyte and leukocyte profile of Santa Inês sheep, with the largest differences between the two sheep categories and lambs.

Objetivou-se avaliar a influência do nível de suplementação com concentrado sobre o perfil hematológico de ovelhas Santa Inês no terço final da gestação e puerpério, assim como dos cordeiros. Utilizaram-se onze ovelhas, aos 75 últimos dias de gestação e nos primeiros 75 dias de lactação e oito cordeiros progênie das ovelhas, distribuídas em delineamento inteiramente casualizado em arranjo de parcelas subdivididas no tempo. As amostras de sangue foram colhidas sempre pela manhã a cada 14 dias. A suplementação não afetou (P>0,05) o eritrograma e o leucograma dos animais e os componentes sanguíneos avaliados encontraram-se dentro do intervalo de referência para a espécie. A categoria animal influenciou (P 0,05) os valores de hemácias, observando-se os maiores valores (11,72 x 106/µL) para os cordeiros. Os cordeiros apresentaram valores inferiores (3073,3/µL) de neutrófilos em comparação com as ovelhas, seja na gestação (4957,6/µL) ou no puerpério (4067,3/µL), que não diferiram entre si (P>0,05). Já na contagem de linfócitos, os valores encontrados nos cordeiros (2858,8/µL) foram semelhantes aos das fêmeas gestantes (2982,0/µL); as fêmeas em lactação (4119,8/µL) apresentaram os maiores valores. A suplementação com concentrado (0,5% do PV) no pré e pós-parto não altera o perfil eritrocitário e leucocitário de ovelhas Santa Inês, sendo as maiores diferenças observadas entre as ovelhas das duas categorias e os cordeiros.

Perfil hematológico de ovelhas santa inês suplementadas a pasto no terço final de gestação e no pós-parto / Hematological profile of Santa Inês ewes supplemented on pasture at the last third of pregnancy and postpartum

The purpose of this study was to evaluate the influence of concentrate supplementation level on hematological profile of Santa Ines ewes, at the last third of pregnancy and postpartum, and of their lambs. We used eleven ewes at the last 75 days of pregnancy and the first 75 days of lactation and eight lambs born from these ewes, allotted in a completely randomized design and split-plot arrangement in time. Blood samples were collected every fourteen days. Neither the erythrocyte nor the leukocyte profile was affected by the different levels of supplementation (P>0.05) and the blood components evaluated were within the reference range for adult sheep. The animal category affected (P 0.05) erythrocytes, and the highest values ??(11.72 x 106/?l) were observed in lambs. Lambs showed lower values ??(3073.3/µL) for neutrophils in comparison with ewes, during pregnancy (4957.6/µL) or postpartum (4067.3/µL); these values did not differ (P>0.05). For the lymphocyte count, the values ??found in lambs (2858.8 /µL) were similar to those in pregnant ewes (2,982.0/µL); lactating ewes (4119.8/µL) showed the highest values. Concentrate supplementation (0.5% BW) at pre and postpartum does not alter the erythrocyte and leukocyte profile of Santa Inês sheep, with the largest differences between the two sheep categories and lambs.(AU)

Objetivou-se avaliar a influência do nível de suplementação com concentrado sobre o perfil hematológico de ovelhas Santa Inês no terço final da gestação e puerpério, assim como dos cordeiros. Utilizaram-se onze ovelhas, aos 75 últimos dias de gestação e nos primeiros 75 dias de lactação e oito cordeiros progênie das ovelhas, distribuídas em delineamento inteiramente casualizado em arranjo de parcelas subdivididas no tempo. As amostras de sangue foram colhidas sempre pela manhã a cada 14 dias. A suplementação não afetou (P>0,05) o eritrograma e o leucograma dos animais e os componentes sanguíneos avaliados encontraram-se dentro do intervalo de referência para a espécie. A categoria animal influenciou (P 0,05) os valores de hemácias, observando-se os maiores valores (11,72 x 106/µL) para os cordeiros. Os cordeiros apresentaram valores inferiores (3073,3/µL) de neutrófilos em comparação com as ovelhas, seja na gestação (4957,6/µL) ou no puerpério (4067,3/µL), que não diferiram entre si (P>0,05). Já na contagem de linfócitos, os valores encontrados nos cordeiros (2858,8/µL) foram semelhantes aos das fêmeas gestantes (2982,0/µL); as fêmeas em lactação (4119,8/µL) apresentaram os maiores valores. A suplementação com concentrado (0,5% do PV) no pré e pós-parto não altera o perfil eritrocitário e leucocitário de ovelhas Santa Inês, sendo as maiores diferenças observadas entre as ovelhas das duas categorias e os cordeiros.(AU)

PERFIL HEMATOLÓGICO DE OVELHAS SANTA INÊS SUPLEMENTADAS A PASTO NO TERÇO FINAL DE GESTAÇÃO E NO PÓS-PARTO

title>Abstract /title> p>The purpose of this study was to evaluate the influence of concentrate supplementation level on hematological profile of Santa Ines ewes, at the last third of pregnancy and postpartum, and of their lambs. We used eleven ewes at the last 75 days of pregnancy and the first 75 days of lactation and eight lambs born from these ewes, allotted in a completely randomized design and split-plot arrangement in time. Blood samples were collected every fourteen days. Neither the erythrocyte nor the leukocyte profile was affected by the different levels of supplementation (P>0.05) and the blood components evaluated were within the reference range for adult sheep. The animal category affected (P 0.05) erythrocytes, and the highest values (11.72 x 106/p.l) were observed in lambs. Lambs showed lower values (3073.3/µL) for neutrophils in comparison with ewes, during pregnancy (4957.6/µL) or postpartum (4067.3/µL); these values did not differ (P>0.05). For the lymphocyte count, the values found in lambs (2858.8 /µL) were similar to those in pregnant ewes (2,982.0/µL); lactating ewes (4119.8/µL) showed the highest values. Concentrate supplementation (0.5% BW) at pre and postpartum does not alter the erythrocyte and leukocyte profile of Santa Inês sheep, with the largest differences between the two sheep categories and lambs. /p>

title>Resumo /title> p>Objetivou-se avaliar a influência do nível de suplementação com concentrado sobre o perfil hematológico de ovelhas Santa Inês no terço final da gestação e puerpério, assim como dos cordeiros. Utilizaram-se onze ovelhas, aos 75 últimos dias de gestação e nos primeiros 75 dias de lactação e oito cordeiros progênie das ovelhas, distribuídas em delineamento inteiramente casualizado em arranjo de parcelas subdivididas no tempo. As amostras de sangue foram colhidas sempre pela manhã a cada 14 dias. A suplementação não afetou (P>0,05) o eritrograma e o leucograma dos animais e os componentes sanguíneos avaliados encontraram-se dentro do intervalo de referência para a espécie. A categoria animal influenciou (P 0,05) os valores de hemácias, observando-se os maiores valores (11,72 x 10 sup>6 /sup>/µL) para os cordeiros. Os cordeiros apresentaram valores inferiores (3073,3/µL) de neutrófilos em comparação com as ovelhas, seja na gestação (4957,6/µL) ou no puerpério (4067,3/µL), que não diferiram entre si (P>0,05). Já na contagem de linfócitos, os valores encontrados nos cordeiros (2858,8/µL) foram semelhantes aos das fêmeas gestantes (2982,0/µL); as fêmeas em lactação (4119,8/µL) apresentaram os maiores valores. A suplementação com concentrado (0,5% do PV) no pré e pós-parto não altera o perfil eritrocitário e leucocitário de ovelhas Santa Inês, sendo as maiores diferenças observadas entre as ovelhas das duas categorias e os cordeiros. /p>

Eritrograma glutationa reduzida e superóxido dismutase eritrocitários e metahemoglobina em equinos da raça Árabe submetidos a exercício em esteira : efeito da suplementação com vitamina E (dl-alfa-tocoferol)

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The objective of this study was to evaluate the oxidative erythrocyte metabolism in equines submitted to exercise on high-speed treadmill and the effect of vitamin E supplementation. Eight adults Arabian horses, males and females, were divided: control group (CG) and group supplemented with vitamin E (EG), (1000 UI/animal/day). All the equines were submitted to exercise with two incremental tests (T1 and T2). Exercise protocol for two tests started with 1.8m/s for 5 min, 4m/s for 3 min, 6m/s for 2 min and right after, periods of 1 min, challenging the equines with increasing speeds until the animals had no condition to keep the exercise on a treadmill inclined at 7%. Between the tests, a training protocol was performed for 20 days. Blood samples were taken before, during, and until 120 hours after exercise to determine erytrogram, erythrocyte reduced glutathione (GSH), superoxide dismutase (SOD), methemoglobin, erythrocyte osmotic fragility (EOF), total plasmatic protein (TPP), seric malondialdehyde (MDA) and vitamin E, seric enzymatic activity of aspartate aminotransferase (AST) and creatine kinase (CK), blood lactate and bloodgas. The results were compared by non-parametric Mann-Whitney and Friedman tests. Although differences were detected in only a few variables, it was possible to see increase in erytrogram and TPP by spleenic contraction and/or decrease of the plasma volume, and increase the erythrocyte volume by swelling. Sport anemia was observed between 24h and 120h after the exercise. The methemoglobin was compatible with the physiological levels and the EOF increased during the exercise in both groups. The MDA elevation confirm the liperoxidation by exercise effect, less intense EG by the supplementation with vitamin E. The exercise also induced increase of AST and CK enzymes, increase of blood lactate and metabolic acidosis

Metabolismo do ferro e lipoperoxidação eritrocitária em equinos puro sangue árabe sumetidos ao exercício em esteira e suplementados com vitamina E (dl-alfa-tocoferol)

O objetivo deste trabalho foi avaliar os efeitos do exercício progressivo em esteira de alta velocidade, do treinamento e da suplementação com vitamina E no metabolismo do ferro e na lipoperoxidação eritrocitária em equinos Puro Sangue Árabe. Foram utilizados 16 animais, distribuídos em dois grupos: controle (GC=8) e suplementado com vitamina E (GE=8) (1.000 U/animal/dia). Os equinos não treinados foram submetidos a uma prova de exercício progressivo (P1). Em seguida, foram treinados por 20 dias e submetidos a uma segunda prova de exercício (P2). O protocolo de exercício para as duas provas iniciou-se a 1,8m/s por 5 min, 4m/s por 3min, 6m/s por 2min seguido de fases de 1min em velocidades crescentes até que os animais conseguissem manter-se em exercício, com a esteira inclinada a +7%. Não houve influência da suplementação com vitamina E ou do treinamento no metabolismo do ferro. O ferro sérico aumentou imediatamente após o exercício por hemoconcentração e houve redução por sequestro após 6h, retornando aos valores basais em 24h. O exercício induziu ?pseudoanemia? entre 6 a 120h após o exercício nos animais não treinados. Não houve alteração na concentração de ferritina sérica. O MDA eritrocitário basal foi maior nos animais treinados e houve maior produção in vitro de MDA eritrocitário nos animais suplementados. O MDA sérico aumentou 30min após o exercício e a concentração de vitamina E sérica não se alterou. Conclui-se que este protocolo de exercício promove alteração da dinâmica de distribuição do ferro, mas não altera o conteúdo total de ferro do organismo. A mensuração do MDA sérico é sensível para avaliar o estresse oxidativo induzido pelo exercício, porém o MDA eritrocitário basal é mais eficiente para avaliar o estresse do treinamento. A suplementação com vitamina E não impediu o estresse oxidativo

This study aimed to evaluate the effects of incremental exercise in high-speed treadmill, training and vitamin E supplementation on iron metabolism and erythrocyte lipoperoxidation in Arabian horses. Sixteen animals were distributed into two groups: control (GC=8) and supplemented with vitamin E (GE=8) (1,000 U/animal/day). The untrained horses were submitted to an incremental exercise trial (P1). Then, they were submitted to a 20 day training period and to a second incremental exercise trial (P2). Exercise protocol for two tests was started with 1.8m/s for 5 min, 4m/s for 3 min, 6m/s for 2 min and right after, periods of 1 min and challenging the equines with increasing speeds until the animals hasn?t no condition to prolong the exercise. There was no influence of vitamin E supplementation or training on iron metabolism. Serum iron levels increased immediately after exercise due to hemoconcentration and there was a reduction by sequestration after 6 h, which returned to basal values in 24 h. The exercise induced a ?pseudoanemia? 6 to 120 h after the exercise in untrained animals. There was no alteration in serum ferritin concentration. Basal erythrocyte MDA was higher in trained animals and there was major in vitro erythrocyte MDA production in supplemented animals. Serum MDA elevated 30 min after exercise and vitamin E concentration did not change. In conclusion, this exercise protocol promotes changes in iron distribution dynamic, but do not change the total iron containing in the organism. Serum MDA measurement is sensitive to evaluate the oxidative stress induced by exercise, however basal erythrocyte MDA is more efficient to evaluate training stress. Vitamin E supplementation not avoids oxidative stress