The specific leaf area of forest species subjected to different degrees of shading did not differ when detached and full leaflets were compared / Folhas compostas de espécies florestais apresentam a mesma área foliar específica com folíolos destacados ou com folhas completas sob sombreamento

Studies of forest species under different environmental conditions are essential for enhancing our understanding of plant-environment relationships. Different light conditions interfere with the production of seedlings within forests, and this interference can be verified by assessing leaf area. However, several species have leaf compound that often contain numerous leaflets, which makes the direct measurement of leaf area difficult. This leads researchers to question whether these types of leaves should be separated for analysis or measured whole. Thus, the aim of this study was to analyse specific leaf area in whole leaves and detached leaflets of six forest species subjected to different levels of shading. The forest species assessed all produced compound leaves and included Schinus terebinthifolius Raddi (aroeira), Schinopsis brasiliensis Engl. (braúna), Dalbergia nigra (Vell.) Fr. All. ex Benth. (jacaranda-da-bahia), Chorisia speciosa A. St.-Hil (paineira), Caesalpinia peltophoroides Benth (sibipiruna) and Plathymenia reticulata Benth (vinhático). Each species was subjected to six different degrees of shading: 0% (full sun); 20; 40; 60; 80 and 90%. Sombrite netting was used to shade plants. The analysis of the specific leaf area was performed using two different methods, which included the analysis of separate leaflets and whole leaves. To analyse leaflet area,50 leaflets per plant were assessed three times, and six plants per treatment were assessed. To assess whole leaves, data were obtained from the whole leaves of six plants per treatment. This process was repeated for all aforementioned species. Data obtained from both analysis methods indicated that all species assessed were similarly affected by shade, and leaf area varied according to the level of shading. This similarity of data demonstrates that, for these species, itis not necessary to use complex methods to obtain specific leaf area measurements. Thus, determining specific [...].

Estudos com espécies florestais, em diferentes condições ambientais são essenciais para o entendimento da relação planta-ambiente. Diferentes condições de luminosidade podem interferir na produção de mudas florestais e o reflexo dessa interferência pode ser verificado pela análise da área foliar. No entanto, muitas espécies apresentam folhas compostas, sendo muitas vezes com inúmeros folíolos, o que dificulta as análises com mensuração direta da área foliar, proporcionando dúvidas durante a análise, se devem ser separados para análise ou mensurados com a folha inteira. Assim, o objetivo desse estudo foi analisar a área foliar específica em folhas inteiras e folíolos destacados de folhas compostas de seis espécies florestais submetidas à diferentes níveis de sombreamento. As espécies florestais utilizadas foram Schinus terebinthifolius Raddi (aroeira), Schinopsis brasiliensis Engl. (braúna), Dalbergia nigra (Vell.) Fr. All. ex Benth. (jacarandá-da-bahia), Chorisia speciosa A.St.-Hil (paineira), Caesalpinia peltophoroides Benth (sibipiruna) e Plathymenia reticulata Benth (vinhático), todas com folhas compostas. Cada espécie foi submetida a seis tratamentos de sombreamento: 0% (pleno sol); 20;40; 60; 80 e 90%, com a utilização de "sombrites" para os tratamentos sombreados. A análise da área foliar específica foi realizada de duas maneiras: folíolos separados e folha inteira. Para a análise dos folíolos esses foram obtidos de três medidas (repetições) de 50 folíolos por planta, realizada em seis plantas por tratamento. Para a análise da folha inteira os dados foram obtidos de folhas inteiras de seis plantas por tratamento. Esse processo se repetiu em todas as espécies supramencionadas. Para todas as espécies, os dados indicaram um padrão de resposta semelhante em ambos os métodos de análise, [...].

The specific leaf area of forest species subjected to different degrees of shading did not differ when detached and full leaflets were compared / Folhas compostas de espécies florestais apresentam a mesma área foliar específica com folíolos destacados ou com folhas completas sob sombreamento

Studies of forest species under different environmental conditions are essential for enhancing our understanding of plant-environment relationships. Different light conditions interfere with the production of seedlings within forests, and this interference can be verified by assessing leaf area. However, several species have leaf compound that often contain numerous leaflets, which makes the direct measurement of leaf area difficult. This leads researchers to question whether these types of leaves should be separated for analysis or measured whole. Thus, the aim of this study was to analyse specific leaf area in whole leaves and detached leaflets of six forest species subjected to different levels of shading. The forest species assessed all produced compound leaves and included Schinus terebinthifolius Raddi (aroeira), Schinopsis brasiliensis Engl. (braúna), Dalbergia nigra (Vell.) Fr. All. ex Benth. (jacaranda-da-bahia), Chorisia speciosa A. St.-Hil (paineira), Caesalpinia peltophoroides Benth (sibipiruna) and Plathymenia reticulata Benth (vinhático). Each species was subjected to six different degrees of shading: 0% (full sun); 20; 40; 60; 80 and 90%. Sombrite netting was used to shade plants. The analysis of the specific leaf area was performed using two different methods, which included the analysis of separate leaflets and whole leaves. To analyse leaflet area,50 leaflets per plant were assessed three times, and six plants per treatment were assessed. To assess whole leaves, data were obtained from the whole leaves of six plants per treatment. This process was repeated for all aforementioned species. Data obtained from both analysis methods indicated that all species assessed were similarly affected by shade, and leaf area varied according to the level of shading. This similarity of data demonstrates that, for these species, itis not necessary to use complex methods to obtain specific leaf area measurements. Thus, determining specific [...].(AU)

Estudos com espécies florestais, em diferentes condições ambientais são essenciais para o entendimento da relação planta-ambiente. Diferentes condições de luminosidade podem interferir na produção de mudas florestais e o reflexo dessa interferência pode ser verificado pela análise da área foliar. No entanto, muitas espécies apresentam folhas compostas, sendo muitas vezes com inúmeros folíolos, o que dificulta as análises com mensuração direta da área foliar, proporcionando dúvidas durante a análise, se devem ser separados para análise ou mensurados com a folha inteira. Assim, o objetivo desse estudo foi analisar a área foliar específica em folhas inteiras e folíolos destacados de folhas compostas de seis espécies florestais submetidas à diferentes níveis de sombreamento. As espécies florestais utilizadas foram Schinus terebinthifolius Raddi (aroeira), Schinopsis brasiliensis Engl. (braúna), Dalbergia nigra (Vell.) Fr. All. ex Benth. (jacarandá-da-bahia), Chorisia speciosa A.St.-Hil (paineira), Caesalpinia peltophoroides Benth (sibipiruna) e Plathymenia reticulata Benth (vinhático), todas com folhas compostas. Cada espécie foi submetida a seis tratamentos de sombreamento: 0% (pleno sol); 20;40; 60; 80 e 90%, com a utilização de "sombrites" para os tratamentos sombreados. A análise da área foliar específica foi realizada de duas maneiras: folíolos separados e folha inteira. Para a análise dos folíolos esses foram obtidos de três medidas (repetições) de 50 folíolos por planta, realizada em seis plantas por tratamento. Para a análise da folha inteira os dados foram obtidos de folhas inteiras de seis plantas por tratamento. Esse processo se repetiu em todas as espécies supramencionadas. Para todas as espécies, os dados indicaram um padrão de resposta semelhante em ambos os métodos de análise, [...].(AU)

Effects of copaiba oil on dermonecrosis induced by Loxosceles intermedia venom

Accidents caused by spiders of the genus Loxosceles constitute an important public health problem in Brazil. The venom of Loxosceles sp induces dermonecrosis at the bite site and systemic disease in severe cases. Traditional medicine based on plant-derived products has been proven to reduce the local effects of envenomation. The present study verified the healing effects of copaiba oil on lesions induced by the venom of L. intermedia. Methods: Cutaneous lesions were induced on the backs of rabbits by intradermal injection of L. intermedia venom. Copaiba oil was applied topically 6 hours after injection; the treatment was repeated for 30 days, after which animal skins were removed and processed for histopathological analysis. Blood samples were also collected before and 24 hours after venom inoculation to measure the hematological parameters. Results: Compared to the control group, the platelet count was reduced significantly in all groups inoculated with venom, accompanied by a decreased number of heterophils in the blood. The minimum necrotic dose (MND) was defined as 2.4 μg/kg. Topical treatment with copaiba oil demonstrated a differentiated healing profile: large skin lesions were observed 10 days after venom inoculation, whereas formation of a thick crust, without scarring was observed 30 days after venom inoculation. Histopathological analysis showed no significant difference after treatment. Nevertheless, the copaiba oil treatment induced a collagen distribution similar to control skin, in marked contrast to the group that received only the spider venom injection. Conclusions: We conclude that copaiba oil may interfere in the healing process and thus propose it as a possible topical treatment for cutaneous lesions induced by L. intermedia venom.(AU)

Effects of copaiba oil on dermonecrosis induced by Loxosceles intermedia venom

Background:Accidents caused by spiders of the genus Loxosceles constitute an important public health problem in Brazil. The venom of Loxosceles sp induces dermonecrosis at the bite site and systemic disease in severe cases. Traditional medicine based on plant-derived products has been proven to reduce the local effects of envenomation. The present study verified the healing effects of copaiba oil on lesions induced by the venom of L. intermedia.Methods:Cutaneous lesions were induced on the backs of rabbits by intradermal injection of L. intermedia venom. Copaiba oil was applied topically 6 hours after injection; the treatment was repeated for 30 days, after which animal skins were removed and processed for histopathological analysis. Blood samples were also collected before and 24 hours after venom inoculation to measure the hematological parameters.Results:Compared to the control group, the platelet count was reduced significantly in all groups inoculated with venom, accompanied by a decreased number of heterophils in the blood. The minimum necrotic dose (MND) was defined as 2.4 μg/kg. Topical treatment with copaiba oil demonstrated a differentiated healing profile: large skin lesions were observed 10 days after venom inoculation, whereas formation of a thick crust, without scarring was observed 30 days after venom inoculation. Histopathological analysis showed no significant difference after treatment. Nevertheless, the copaiba oil treatment induced a collagen distribution similar to control skin, in marked contrast to the group that received only the spider venom injection.Conclusions:We conclude that copaiba oil may interfere in the healing process and thus propose it as a possible topical treatment for cutaneous lesions induced by L. intermedia venom.(AU)