Nutrient digestibility in horses of tropical grasses found in semi-arid areas of the Brazilian Northeast region assessed using mobile bags

The present study used mobile bags to estimate horse nutrient digestibility of tropical grasses found in semi-arid areas of the Brazilian Northeast region. Five female mixed-breed horses with a mean weight of 400±23 kg were assigned to a 5×5 Latin square design with five periods of seven days and five grasses: Tifton 85 hay (Cynodon spp.), sixweeks threeawn (Aristida adscensionis Linn.), Alexandergrass (Brachiaria plantaginea (Link) Hitchc), capim-de-raiz (Chloris orthonoton Doell), and Sabi grass (Urochloa mosambicensis). The nutrient content of forages was determined prior to inoculation in horses and after recovery of mobile bags from feces. The digestibility coefficients were determined from the difference between the inoculated and recovered material. The dry matter, organic matter, mineral matter, crude protein, neutral detergent fiber, and acid detergent fiber contents of the grass species were analyzed. Digestibility data were subjected to analysis of variance using the Statistical Analysis System (SAS, version 9.0) software. Higher dry matter digestibility coefficients were observed in Tifton 85 (74.61%), Alexandergrass (74.30%), and capim-de-raiz (68.88%) than in sixweeks threeawn (48.40%) and Sabi grass (52.89%). The highest crude protein digestibility coefficients were found for Alexandergrass (95.70%), Tifton 85 (93.50%), and sixweeks threeawn (93.35%). Sixweeks threeawn had lower apparent mineral matter digestibility than the other grasses. The digestibility coefficients of Alexandergrass and capim-de-raiz indicate that those grasses have potential to be used in equine feed.

Uso de hCG e deslorelina na indução de ovulação de éguas cíclicas no Agreste de Pernambuco / Use of hCG and deslorelin in mares ovulation induction in Agreste of Pernambuco

The experiment aimed to compare the efficiency of deslorelin and hCG to induce ovulation Quarter Horsemares. This experiment was conducted at the Center for Equine Pernambuco - CEPE, in the town of Gravatá-PE, in February 2013. The animals were divided in Group 1 (n=10) received, intramuscular, Deslorelin 1000mg while Group 2 (n = 10) received, Intravenous, 5000 IU of hCG. 24 hours after administration of theovulation inducing were inseminated with semen from a stallion. After 15 days of AI, the pregnancy diagnosiswas made. The data from this study were analyzed using the statistical package SAS, (P < 0.05). The fertilityrate found was 80.0% in group 1 and 70% in group 2 (P > 0.05). The drugs tested successfully induce ovulation.(AU)

Uso de hCG e deslorelina na indução de ovulação de éguas cíclicas no Agreste de Pernambuco / Use of hCG and deslorelin in mares ovulation induction in Agreste of Pernambuco

The experiment aimed to compare the efficiency of deslorelin and hCG to induce ovulation Quarter Horsemares. This experiment was conducted at the Center for Equine Pernambuco - CEPE, in the town of Gravatá-PE, in February 2013. The animals were divided in Group 1 (n=10) received, intramuscular, Deslorelin 1000mg while Group 2 (n = 10) received, Intravenous, 5000 IU of hCG. 24 hours after administration of theovulation inducing were inseminated with semen from a stallion. After 15 days of AI, the pregnancy diagnosiswas made. The data from this study were analyzed using the statistical package SAS, (P 0.05). The drugs tested successfully induce ovulation.

Use of glutathione peroxidase and cysteine in Botu-Sêmen® and ACP-105® diluents on integrity of chilled equine spermatozoa / Uso de glutationa peroxidase e cisteína aos diluentes botusêmen® e ACP-105® na integridade de espermatozoides equinos refrigerados

Aiming to study the effect of glutathione peroxidase (GPx) and cysteine (Cis) addition in Botu-Sêmen® and ACP-105® diluents on integrity of equine spermatozoa, semen samples were cooling (14 C) and analyzed for total motility (TM), vigor, membrane integrity (iM) and acrosome (iAC), and mitochondrial membrane potential (MMP), after 0 (T0), 12 (T12) and 24 (T24) hours of cooling. Analysis of TM in T12 demonstrated that semen samples diluted in Botu-sêmen® (G1) and Botu-sêmen®+GPx+ Cis (G7) had higher (P 0.05) percentage than those diluted in ACP-105®+ Cis (G6). At T24, samples diluted in G7 had higher MT (P 0.05) than those in G2 and G6. Sperm vigor in T12 was higher (P<0.05) in samples diluted at G7 than in G2 and G6. At T24, the vigor of samples diluted G4 and G7 was higher (P<0.05) than inG2 and G6. No significant difference were observed to iM and MMP among groups, except to MMP at T0 was higher (P<0.05) in G1 than G7 and G8. At T24 the iAC parameter was higher (P<0.05) in the G6 than in G1. It can be concluded that GPx (5U) and Cis (5mM) in association preserves equine spermatozoa diluted in Botu-sêmen® at 14C during 24 hours.(AU)

Visando estudar o efeito da adição de glutationa peroxidase (GPx) e cisteína (Cis) aos diluentes Botu-Sêmen® e ACP-105® na viabilidade de espermatozoides equinos, amostras de sêmen foram refrigeradas (14 ºC) e analisadas quanto à motilidade total (MT), vigor, integridade de membrana (iM) e de acrossoma (iAc), e potencial da membrana mitocondrial (PMM), após0 (T0), 12 (T12) e 24 (T24) horas de refrigeração. A análise da MT evidenciou no T12 que as amostras de sêmen dos grupos Botu-sêmen® (G1) e Botu-sêmen®+GPx+Cist (G7) apresentaram maiores (P<0,05) percentuais do que no grupo ACP-105®+Cist (G6). No T24, as amostras do G7 apresentaram maior (P 0,05) MT do que as diluídas em ACP-105® (G2) e ACP-105®+- Cist (G6). O vigor espermático no T12 foi maior (P<0,05) no G7 do que no G2 e G6. No T24, o vigor das amostras do G4 e G7 foi maior (P<0,05) do que nas do G2 e G6. A iM e o PMM não diferiram (P>0,05) entre grupos, com exceção do PMM que no T0 foi maior (P<0,05) no G1 do que no G7 e no G8. A iAc no T24 foi maior (P<0,05) no G6 do que no G1. Conclui-se que a associação de GPx (5 U) e Cist (5 mM) preserva espermatozoides equinos diluídos em Botu-sêmen® refrigerados (14 oC) durante 24 horas.(AU)

Use of glutathione peroxidase and cysteine in Botu-Sêmen® and ACP-105® diluents on integrity of chilled equine spermatozoa / Uso de glutationa peroxidase e cisteína aos diluentes botusêmen® e ACP-105® na integridade de espermatozoides equinos refrigerados

Aiming to study the effect of glutathione peroxidase (GPx) and cysteine (Cis) addition in Botu-Sêmen® and ACP-105® diluents on integrity of equine spermatozoa, semen samples were cooling (14 C) and analyzed for total motility (TM), vigor, membrane integrity (iM) and acrosome (iAC), and mitochondrial membrane potential (MMP), after 0 (T0), 12 (T12) and 24 (T24) hours of cooling. Analysis of TM in T12 demonstrated that semen samples diluted in Botu-sêmen® (G1) and Botu-sêmen®+GPx+ Cis (G7) had higher (P 0.05) percentage than those diluted in ACP-105®+ Cis (G6). At T24, samples diluted in G7 had higher MT (P 0.05) than those in G2 and G6. Sperm vigor in T12 was higher (P<0.05) in samples diluted at G7 than in G2 and G6. At T24, the vigor of samples diluted G4 and G7 was higher (P<0.05) than inG2 and G6. No significant difference were observed to iM and MMP among groups, except to MMP at T0 was higher (P<0.05) in G1 than G7 and G8. At T24 the iAC parameter was higher (P<0.05) in the G6 than in G1. It can be concluded that GPx (5U) and Cis (5mM) in association preserves equine spermatozoa diluted in Botu-sêmen® at 14C during 24 hours.

Visando estudar o efeito da adição de glutationa peroxidase (GPx) e cisteína (Cis) aos diluentes Botu-Sêmen® e ACP-105® na viabilidade de espermatozoides equinos, amostras de sêmen foram refrigeradas (14 ºC) e analisadas quanto à motilidade total (MT), vigor, integridade de membrana (iM) e de acrossoma (iAc), e potencial da membrana mitocondrial (PMM), após0 (T0), 12 (T12) e 24 (T24) horas de refrigeração. A análise da MT evidenciou no T12 que as amostras de sêmen dos grupos Botu-sêmen® (G1) e Botu-sêmen®+GPx+Cist (G7) apresentaram maiores (P0,05) entre grupos, com exceção do PMM que no T0 foi maior (P<0,05) no G1 do que no G7 e no G8. A iAc no T24 foi maior (P<0,05) no G6 do que no G1. Conclui-se que a associação de GPx (5 U) e Cist (5 mM) preserva espermatozoides equinos diluídos em Botu-sêmen® refrigerados (14 oC) durante 24 horas.