Trypanosoma vivax em bovino leiteiro / Trypanosoma vivax in dairy cattle

Background: Trypanosoma vivax is a protozoan that causes reproductive disorders and decreased production in domestic and wild ungulate animals. The bovine are the main hosts of the disease and the transmission occurs by the bite of hematophagous insects, mainly tabanids. Several diagnostic techniques can be used to detect the parasite, both in parasitological form and by serological kits. In Brazil, the disease has been reported in bovines, goats and sheep of some states, with high morbidity and mortality and due to the scarcity of results on the epidemiology of the disease, this work had the objective to report the presence of T. vivax in a female bovine of a dairy herd in Parnaíba county, Piauí.Case: The animal naturally infected by Trypanosoma vivax, was a three-year-old cow from a dairy farm in the Parnaíba county, located in the north of Piauí state. The farm had a herd whith 62.20% of young Girolando breed cows and the breeding system used was semi-confinement, with two mechanical milking per day. At the time of a Veterinarian’s technical visit to the property, it was observed the occurrence of abortions, mastitis, estrus repetitions and cows with hematuria, leading to the suspicion of the bovine leptospirosis occurrence. Blood samples were collected from 78 cows from the herd for hematological, biochemical and serological tests, and 72 (92.30%) were reactive to some Leptospira serovars. All the exams were carried out at the Federal University of Piauí (UFPI). In the group of animals negative for leptospirosis, a female was diagnosed positive for bovine trypanosomiasis, confirming the result in the blood smear. This animal had no clinical signs characteristic of the disease at the time of the evaluation.[...]

Trypanosoma vivax em bovino leiteiro / Trypanosoma vivax in dairy cattle

Background: Trypanosoma vivax is a protozoan that causes reproductive disorders and decreased production in domestic and wild ungulate animals. The bovine are the main hosts of the disease and the transmission occurs by the bite of hematophagous insects, mainly tabanids. Several diagnostic techniques can be used to detect the parasite, both in parasitological form and by serological kits. In Brazil, the disease has been reported in bovines, goats and sheep of some states, with high morbidity and mortality and due to the scarcity of results on the epidemiology of the disease, this work had the objective to report the presence of T. vivax in a female bovine of a dairy herd in Parnaíba county, Piauí.Case: The animal naturally infected by Trypanosoma vivax, was a three-year-old cow from a dairy farm in the Parnaíba county, located in the north of Piauí state. The farm had a herd whith 62.20% of young Girolando breed cows and the breeding system used was semi-confinement, with two mechanical milking per day. At the time of a Veterinarians technical visit to the property, it was observed the occurrence of abortions, mastitis, estrus repetitions and cows with hematuria, leading to the suspicion of the bovine leptospirosis occurrence. Blood samples were collected from 78 cows from the herd for hematological, biochemical and serological tests, and 72 (92.30%) were reactive to some Leptospira serovars. All the exams were carried out at the Federal University of Piauí (UFPI). In the group of animals negative for leptospirosis, a female was diagnosed positive for bovine trypanosomiasis, confirming the result in the blood smear. This animal had no clinical signs characteristic of the disease at the time of the evaluation.[...](AU)

Detecção de Brucella ovis por reação em cadeia da polimerase (PCR) em amostras de urina de ovinos naturalmente infectados na microrregião de Teresina, PI / Brucella ovis detection by polymerase chain reaction (PCR) in naturally infected sheep urine samples in the micro region of Teresina, PI

Ovine Brucellosis is an infectious disease responsible for the production of reproductive disorders,triggering economic losses to sheep production. The researchs objective was detect B. ovis DNA in sheepsbelonging to cities in the micro region of Teresina-PI. Thus, were collected 100 urine and blood samples ofsheeps older than or equal to six months. Urine samples were subjected to conventional PCR and samples ofblood AGID technique. From 100 blood samples, 17 (17%) were reactive to AGID. In conventional PCR, from100 urine samples, six (6%) were positive. There was agreement between the tests, however, was consideredweak (18.9%), based on the Kappa coefficient ranking. Thus, it was observed that both techniques are valid and can be regarded as tools of choice for the diagnosis of ovine brucellosis.(AU)

Soroprevalência de anticorpos Anti-Brucella abortus em rebanhos bovinos da microrregião de Teresina, Piauí / Seroprevalence Anti-Brucella abortus antibodies in cattle herds in the microregion of Teresina, Piauí

The objective of this study is to estimate the prevalence in cattle herds of the micro-region of Teresina,state of Piauí. It was collected 406 serum samples from 14 herds, using as a screening technique buffered plateagglutination test (BPAT). Of the 406 samples, 15 (3.69%) responded to the BPAT, from the 14 herds studied,five showed at least one positive animal, given the prevalence of outbreaks of 35.7% (5/14). The results suggestthat the disease is widespread in herds and could trigger a spreading agent in the region, requiring thatprophylactic measures should be instituted by related official agencies, in maintaining the health of livestock.(AU)

Avaliação das técnicas, imunodifusão em gel de agar (IDGA), ensaio imunoenzimático (ELISAi) e reação em cadeia da polimerase (PCR), para o diagnostico da brucelose ovina / Evaluation of technical, immunodiffusion agar gel (AGID), enzyme-linked immunosorbent assay (ELISAi) and polymerase chain reaction (PCR) for the diagnosis of ovine brucellosis

Brucellosis in sheep has received a major focus, since it is a disease that affects the reproductive systemof animals, causing serious impairment in the productive sector. Thus, three methods for the diagnosis of ovinebrucellosis were evaluated as goal, the indirect ELISA test, the AGID technique and the PCR. Therefore, weused 211 sheep blood samples arising from properties of nine municipalities of the homogeneous micro-regionof Teresina, Piaui. The 211 blood samples were subjected to the serologic testing and PCR to detect anti-B. ovisantibodies, and Brucella ovis DNA, respectively. Positive results in serological tests were obtained, 36 (17.06%)positive in the AGID test and seven (3.31%) positive to the ELISA test, however, there were no positive results inthe PCR technique. The use of the techniques for the diagnosis of B. ovis infection is satisfactory, but the choiceof blood as the biological sample for performing the direct diagnosis of ovine brucellosis is not recommended.(AU)

Taxa de fertilidade de ovelhas submetidas à inseminação artificial por via cervical e laparoscopia, no semi-árido do Piauí / Fertility rate of ewes submitted to artificial insemination by laparoscopy and cervical, on semi-arid in Piauí

There is various techniques of artificial insemination, but are not yet applied routinely in sheep. Thecervical insemination is quick and simple. Laparoscopy requires specialized technical and more work. artificialinsemination using simultaneous estrus synchronization provides higher productivity. This study aimed toobserve the pregnancy rate using frozen semen and techniques of different insemination after synchronization ofestrus. The experiment was conducted with thirty-seven animals. All subject to estrus synchronization protocol.Sixty days after insemination all underwent ultrasound for diagnosis of pregnancy. Twenty-five of the thirtysevenof the experiment showed pregnancy. The results demonstrated that cervical AI with frozen semen is agood alternative to using it routinely in sheep.(AU)

Fertilidade do sêmen congelado/descongelado e, posteriormente, rediluído em acp-102 em ovinos de corte exploradas em sistema de criação semi-intensivo / Fertility of frozen / thawed semen and subsequently rediluted in ACP-102® sheep sectional operated in semiintensive farming system

The objective of this study was the evaluation of the frozen and thawed semen fertility in ACP-102®.For this, 37 ewes were used, previously evaluated and considered clinically and reproducibly fit according toCBRA standards. The semen used was also evaluated according to CBRA standards (2013). After beingcollected, the semen was frozen in a TK 3000® seminal freezing machine. After thawing, half of the sampleswere re-diluted in ACP-102®, each vial in a 1 ml aliquot of ACP, and then the animals were inseminated indifferent ways, laparoscopic and transcervical. The group of animals submitted to insemination with semen redilutedin ACP-102® demonstrated a fertility rate on the 60-day ultrasound examination greater than the groupinseminated with Tris-Gem alone. No significant differences were found between insemination techniquesregarding fertility.(AU)

Influencia da concentração espermática sobre a Taxa de Fertilidade em Ovelhas Inseminadas com Sêmen congelado / Influences of sperm concentration on Fertility Rate in Sheep inseminated with frozen semen

The experiment compared the pregnancy rates in sheep submitted to IA, to evaluate the influence ofsperm concentration on fertility rate in sheep inseminated with frozen semen. In experiment run 37 females wereused, subject to synchronization of estrus and divided into two groups: G1 (n = 19), using artificial inseminationwith a concentration of 200 million viable spermatozoa and G2 (n = 18), using artificial insemination is at aconcentration of 100 million sperm. Pregnancy rates were 66.66% and 77.77% in sheep that were inseminatedwith the concentration 200 and 100 million sperm respectively transcervical and the sheep that have beeninseminated by laparoscopy at the same concentrations as pregnancy rates were 50% and 77.77% respectively.The concentrations used have shown - is quite effective when compared pregnancy rates in each group. Butneeds more in vivo studies.(AU)

Soroprevalência de anticorpos Anti-Brucella abortus em rebanhos bovinos da microrregião de Teresina, Piauí / Seroprevalence Anti-Brucella abortus antibodies in cattle herds in the microregion of Teresina, Piauí

The objective of this study is to estimate the prevalence in cattle herds of the micro-region of Teresina,state of Piauí. It was collected 406 serum samples from 14 herds, using as a screening technique buffered plateagglutination test (BPAT). Of the 406 samples, 15 (3.69%) responded to the BPAT, from the 14 herds studied,five showed at least one positive animal, given the prevalence of outbreaks of 35.7% (5/14). The results suggestthat the disease is widespread in herds and could trigger a spreading agent in the region, requiring thatprophylactic measures should be instituted by related official agencies, in maintaining the health of livestock.

Avaliação das técnicas, imunodifusão em gel de agar (IDGA), ensaio imunoenzimático (ELISAi) e reação em cadeia da polimerase (PCR), para o diagnostico da brucelose ovina / Evaluation of technical, immunodiffusion agar gel (AGID), enzyme-linked immunosorbent assay (ELISAi) and polymerase chain reaction (PCR) for the diagnosis of ovine brucellosis

Brucellosis in sheep has received a major focus, since it is a disease that affects the reproductive systemof animals, causing serious impairment in the productive sector. Thus, three methods for the diagnosis of ovinebrucellosis were evaluated as goal, the indirect ELISA test, the AGID technique and the PCR. Therefore, weused 211 sheep blood samples arising from properties of nine municipalities of the homogeneous micro-regionof Teresina, Piaui. The 211 blood samples were subjected to the serologic testing and PCR to detect anti-B. ovisantibodies, and Brucella ovis DNA, respectively. Positive results in serological tests were obtained, 36 (17.06%)positive in the AGID test and seven (3.31%) positive to the ELISA test, however, there were no positive results inthe PCR technique. The use of the techniques for the diagnosis of B. ovis infection is satisfactory, but the choiceof blood as the biological sample for performing the direct diagnosis of ovine brucellosis is not recommended.

Detecção de Brucella ovis por reação em cadeia da polimerase (PCR) em amostras de urina de ovinos naturalmente infectados na microrregião de Teresina, PI / Brucella ovis detection by polymerase chain reaction (PCR) in naturally infected sheep urine samples in the micro region of Teresina, PI

Ovine Brucellosis is an infectious disease responsible for the production of reproductive disorders,triggering economic losses to sheep production. The research’s objective was detect B. ovis DNA in sheepsbelonging to cities in the micro region of Teresina-PI. Thus, were collected 100 urine and blood samples ofsheeps older than or equal to six months. Urine samples were subjected to conventional PCR and samples ofblood AGID technique. From 100 blood samples, 17 (17%) were reactive to AGID. In conventional PCR, from100 urine samples, six (6%) were positive. There was agreement between the tests, however, was consideredweak (18.9%), based on the Kappa coefficient ranking. Thus, it was observed that both techniques are valid and can be regarded as tools of choice for the diagnosis of ovine brucellosis.

Fertilidade do sêmen congelado/descongelado e, posteriormente, rediluído em acp-102 em ovinos de corte exploradas em sistema de criação semi-intensivo / Fertility of frozen / thawed semen and subsequently rediluted in ACP-102® sheep sectional operated in semiintensive farming system

The objective of this study was the evaluation of the frozen and thawed semen fertility in ACP-102®.For this, 37 ewes were used, previously evaluated and considered clinically and reproducibly fit according toCBRA standards. The semen used was also evaluated according to CBRA standards (2013). After beingcollected, the semen was frozen in a TK 3000® seminal freezing machine. After thawing, half of the sampleswere re-diluted in ACP-102®, each vial in a 1 ml aliquot of ACP, and then the animals were inseminated indifferent ways, laparoscopic and transcervical. The group of animals submitted to insemination with semen redilutedin ACP-102® demonstrated a fertility rate on the 60-day ultrasound examination greater than the groupinseminated with Tris-Gem alone. No significant differences were found between insemination techniquesregarding fertility.

Influencia da concentração espermática sobre a Taxa de Fertilidade em Ovelhas Inseminadas com Sêmen congelado / Influences of sperm concentration on Fertility Rate in Sheep inseminated with frozen semen

The experiment compared the pregnancy rates in sheep submitted to IA, to evaluate the influence ofsperm concentration on fertility rate in sheep inseminated with frozen semen. In experiment run 37 females wereused, subject to synchronization of estrus and divided into two groups: G1 (n = 19), using artificial inseminationwith a concentration of 200 million viable spermatozoa and G2 (n = 18), using artificial insemination is at aconcentration of 100 million sperm. Pregnancy rates were 66.66% and 77.77% in sheep that were inseminatedwith the concentration 200 and 100 million sperm respectively transcervical and the sheep that have beeninseminated by laparoscopy at the same concentrations as pregnancy rates were 50% and 77.77% respectively.The concentrations used have shown - is quite effective when compared pregnancy rates in each group. Butneeds more in vivo studies.

Taxa de fertilidade de ovelhas submetidas à inseminação artificial por via cervical e laparoscopia, no semi-árido do Piauí / Fertility rate of ewes submitted to artificial insemination by laparoscopy and cervical, on semi-arid in Piauí

There is various techniques of artificial insemination, but are not yet applied routinely in sheep. Thecervical insemination is quick and simple. Laparoscopy requires specialized technical and more work. artificialinsemination using simultaneous estrus synchronization provides higher productivity. This study aimed toobserve the pregnancy rate using frozen semen and techniques of different insemination after synchronization ofestrus. The experiment was conducted with thirty-seven animals. All subject to estrus synchronization protocol.Sixty days after insemination all underwent ultrasound for diagnosis of pregnancy. Twenty-five of the thirtysevenof the experiment showed pregnancy. The results demonstrated that cervical AI with frozen semen is agood alternative to using it routinely in sheep.